1. Antibacterial impact: Usnic acid can properly inhibit the development of Gram-positive microorganisms such as Staphylococcus aureus, MRSA, acne bacilli, and coryneform bacteria, as well as can prevent the development of Pityrosporum furfur, directly killing Staphylococcus aureus cells, impeding the transduction path of Pseudomonas aeruginosa signal, recommending that Usnea grandis essence as well as Usnea acid can be made use of to deal with acne, rosacea, seborrheic dermatitis and also various other relevant skin diseases; on top of that, Usnea acid can remove sa drug-resistant plasmid, the function of 24h its removal price of 52%, to prolong the action time to 48h, the removal price of approximately 14.6%, might be utilized for clinical treatment of drug-resistant sa infection.
2. Antiviral impact: Research studies have shown that usnic acid has the possible to hinder the spreading of mouse polyomavirus, primarily by hindering the formation of carrier RNA, thus indirectly hindering the duplication of DNA. Usnenic acid in Usnea longus has the task of hindering Epstein-barrvirus (Epstein-barrvirus, EBV), amongst which (+)-usnicic acid showed the highest inhibitory activity (ED501.0 μg/ mL), while (-)-Usnic acid has weak repressive task (ED505.0 μg/ mL).
3. Insecticidal effect: (+)-usnic acid has the activity of killing the promastigotes of Leishmania donovani (Kala-azar), and the artificial insemination active focus of (+)-usnic acid is 25 μg/ mL. The minimum reliable concentration of S-(-) usnic acid to kill trichomoniasis in vitro is 0.4 mg/mL, and there is no significant distinction in the in vitro trichomoniasis killing impact between usnic acid at a focus of 0.4 mg/mL as well as over as well as the same focus of metronidazole. Usnic acid has a development repressive result on trypanosome larvae in 5-30μg/ mL, as well as usnic acid can likewise substantially minimize the quantity of trypanosomes in the blood. Use 40 or 80μg/ mL usnic acid to act as a gigantic infection. Phagocytes can bring about the disintegration of trypanosome mitochondria and Golgi apparatus, as well as the cytoplasm ends up being vacuolated, but has no impact on host cells.
4. Anti-tumor impact: Usnic acid has anti-wild-type P53 breast cancer cells cell line MCF7, non-functional P53 breast cancer cell line MDA-MB-231, lung cancer cells cell line H1299 as well as different cancer cells cell lines L1210, 3LL, DU145, The activity of MCF7, K-562 as well as U251 does not trigger damages to the DNA of typical cells, so it is anticipated to be turned into a new generation of anticancer medicines. Study the inhibitory result of usnic acid drawn out from the fungus Usnea on the spreading of prostate cancer cells PC-3M cells cultured artificial insemination. The results of cell growth restraint experiments reveal that the maximum inhibition price can reach more than 80%, as well as the biological effect is dose-dependent inhibition Partnership, showing that usnic acid has a repressive effect on PC-3M cells cultured in vitro, decreases the proliferation of growth cells, as well as has evident anti-tumor impacts. Yet usnic acid has no repressive impact on 12(S)-LOX cultured with human platelets in vitro. Usnic acid has poor water solubility, and it makes use of 10%HP-β-CD as a solubilizer to examine the effect on 3 type of human deadly cell lines under the problem of pH7.4. -70-fold boost in solubility in CD, with EC50 of 2.9 μg/ mL, 4.3 μg/ mL as well as 8.2 μg/ mL for T-47D, Panc-1 and also PC-3 cells, respectively.
5. Anti-inflammatory task: Usnic acid was studied on the anti-inflammatory activity of rat paw swelling test (intense swelling) as well as rat cotton sphere granuloma test (persistent swelling), and also advil was made use of as a favorable control drug. The outcomes showed that For intense inflammation, at the same dosage as advil (oral 100mg/kg), the anti-inflammatory effect of ibuprofen is far better than that of usnic acid; for persistent inflammation, the anti-inflammatory result of the two is equivalent, and there is a significant difference compared with the control group, It is guessed that the anti-inflammatory system of pinic acid is associated with the restraint of prostaglandin synthesis.
6. Other activities: research the result of sodium usnelate on promoting skin wound healing. The overall experiment reveals that outside application of sodium usnelate can increase the repair service of speculative large skin damages, however the impact of 0.5-8 μg/ mL on the spreading of L929 fibroblasts No assistance. Additionally, the result of salt usnenate on fibroblasts and also its system of promoting injury healing were examined. The outcomes showed that salt usnenate might promote the secretion of TGF-1 and EGF by fibroblasts, and with the boost of medicine focus As well as enhanced, it generates chemotactic effect on repair cells and also inflammatory cells, promotes repair service cell migration as well as deposition of extracellular matrix, as well as promotes injury healing. Furthermore, experimental studies have actually shown that usnic acid has a better stomach protection effect on the stomach ulcer model of rats induced by indomethacin. Compared with the model team, it can substantially boost the degrees of SOD, GSH as well as GPx in rats, reduce the level of LPO, and boost cNOS, decrease iNOS. In regards to anti-radiation, the research study on the absorption of lichen secondary metabolites in the UV region reveals that usnic acid generally absorbs in the UV-B region as well as is fairly steady to light. And the photolysis item of usnic acid still has absorption effect on UV, recommending that usnic acid is a very possible natural sun block agent, which can be used in sun block and also various other cosmetics.
