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Zhishang Chemical

Trypsin Sigma CAS 9002-07-7

Molecular Formula: C35H47N7O10

Formula Weight: 725.78858

ZSpharmac: Trypsin Sigma Supplement

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Product Name: Trypsin Sigma
CAS No: 9002-07-7
Purity: 99%

Basic Info

Product Name:Trypsin Sigma
Other Names:Trypsin
CAS:9002-07-7
Place of Origin:Shandong, China
Brand Name:ZSpharmac
Type:Pharmaceutical Raw Materials
Appearance:White Powder Lyophilized Powder
EINECS No.:232-650-8
Storage:-20°C
Provide:Trypsin Sigma MSDS;
Trypsin Sigma COA

What is Trypsin Sigma?

The molecular weight of Trypsin Sigma is about 24,000, and its isoelectric point is about pH 8.9. The molecular weight of trypsin is close to its zymogen (23,300), its isoelectric point is about pH 10.8, and its optimum pH is 7.6 to 8.0. It is the most stable at pH=3. When the pH is lower than this pH, Trypsin Sigma is volatile, and it is easy to dissolve when pH>5; Ca2+ ion has a stabilizing effect on trypsin.
Trypsin Sigma can catalyze the hydrolysis of proteins, and has a high degree of specificity for the bonds formed by the carboxyl groups of basic amino acids (arginine, lysine) and the amino groups of other amino acids. In addition, it can also catalyze the amide bond or ester bond formed by the basic amino acid and the carboxyl group, and its high specificity is still manifested in the selection of one end of the basic amino acid.
As a type of protease, Trypsin Sigma not just functions as a digestive system enzyme, yet additionally restricts as well as turns on the precursors of various other enzymes such as chymotrypsinogen, carboxypeptidase, as well as phospholipase.
Additionally, it has anti-inflammatory results. It is medically utilized for neighborhood edema, hematoma and abscess caused by empyema, hemothorax, medical swelling, abscess, distressing injury, fistula, etc.

Trypsin Sigma Properties:

Melting point 115°C
storage temp. -20°C
solubility Reconstitute in aqueous buffer
pkapK1:6.25 (25°C,μ=0.1)
form lyophilized powder
color White powder
OdorOdorless
Water Solubility Soluble in water (10 mg/ml), phosphate buffers (10 mg/ml), and balanced salt solutions (1 mg/ml).

 

Trypsin Sigma Uses

  1. Trypsin Sigma is utilized for biochemical study, protein framework analysis as well as sequence study, healthy protein digestion as well as decomposition. Diseases such as emphysema and bronchitis.
  2. Trypsin Sigma is utilized for numerous surgical ulcers and edema and parasites brought on by swollen gangrene, terrible azole injury, fistula holes, and so on.
  3. Trypsin Sigma works as a proteolytic enzyme. Inner medication is primarily utilized for empyema, emphysema, bronchitis, bronchial asthma blood clot. However, it may cause unfavorable responses such as chills, fever, headache, chest discomfort, abdominal pain, dyspnea, breakout, angioedema, raised intraocular pressure, and leukopenia, as well as seldom anaphylactic shock. The intramuscular shot website is prone to discomfort and also induration. It can not be made use of for hemorrhaging tooth cavity, severe inflammation, and clients with pulmonary hemorrhage within one week. Usage with care in people with tuberculous empyema and also tracheopleural fistula. It is contraindicated in patients with liver as well as kidney damage, abnormal blood coagulation function as well as bleeding. Before use, include an ideal quantity of sodium chloride shot to liquify. Intramuscular injection: regional injection or spray breathing, the dose depends upon the condition.
  4. Trypsin Sigma is readily available as an enzyme prep work. Generally utilized for toasting, meat tenderization, healthy protein hydrolysis, etc.
  5. Trypsin Sigma has the exact same chemical residential properties as animal-derived trypsin. It can be used as opposed to trypsin, as well as has several benefits, such as no miscellaneous enzyme task, high stability, no self-cleavage, high activity; in operation, no non-specific enzyme bosom pieces as well as self-cleavage fragments show up. It is utilized for particular protein food digestion, healthy protein sequencing, making peptide maps, proteomics research study, trypsin food digestion of peptides on Z-D gel, and so on.

Trypsin Sigma Preparation

1. Trypsin crude production operation:
1. Extraction process:
1.1 For fresh pancreas, use forceps and scissors to remove the fatty connective tissue, etc., mince it in a mincer, and weigh 15Kg;
1.2 Put the minced material in a plastic bucket, soak it with 30L of 0.125mol/L sulfuric acid solution for 24 hours, and stir it once per hour during the soaking period;
1.3 Put the impregnated material into a filter bag for filtration, and then soak the filter residue with 15 L of 0.125mol/L sulfuric acid solution for 1 hour, and then discard the filter residue after filtration;
2. Salt dissolving and salting out process:
2.1 Combine the two dipping liquids with a volume of 44.9L, put them in a plastic bucket, add 242g of solid ammonium sulfate to every 1000ml of the solution to reach 40% saturation, add a total of 10.866Kg of solid ammonium sulfate, and let stand for 12 hours;
2.2 Filter, keep the filtrate, the filtrate volume is 44L, and the solid is discarded;
2.3 Add 205g of solid ammonium sulfate to every 1000ml of clear liquid to reach 70% saturation, share 9.020kg of ammonium sulfate, and let stand for 12 hours;
2.4 Filter, keep the filter cake, weigh 269.2g, and discard the filtrate;
2.5 Dissolve with 807.6ml of water, and then add solid ammonium sulfate to precipitate at nearly 40% and 70% saturation according to steps 2.1, 2.2, 2.3, and 2.4;
2.6 Take 70% saturation precipitate, weigh 178.5g, dissolve it in 267.8ml water, add 89.3ml saturated ammonium sulfate solution, and adjust the pH to 5.00 with 5mol/L sodium hydroxide solution;
2.7 The solution was left to stand in an incubator at 25°C for 48 hours, and a needle-shaped crude chymotrypsin product crystallized out;
2.8 Filtration, the mother liquor was adjusted to pH 3.0 with 0.25mol/L sulfuric acid, its volume was 328.4ml, 100.8g of ammonium sulfate was added, and it was allowed to stand for 12 hours;
3. Drying process:
The precipitate was taken and dried in a vacuum drying oven to obtain the crude trypsin product, weighing 100.0 g, that is, 6.67 g of trypsin per kilogram of pancreas.
2. Trypsin refining production operation:
1. Feeding
1.1 Weigh 100.0 g of crude trypsin, dissolve it with 300 ml of purified water, add 2.5 mol/L sulfuric acid solution to adjust the pH to 3.0 to help it dissolve, and stir for 12 hours;
1.2 Measure the volume of the solution to 298.4ml, add 140.9g of solid ammonium sulfate, and after it dissolves, let it stand for precipitation for 12 hours;
1.3 The solution was vacuum filtered, the filter cake weight was weighed 96.6g, 289.9ml of purified water was added, after stirring and dissolving, 2.5mol/L sulfuric acid solution was added to adjust pH to 3.0, 193.2ml of saturated ammonium sulfate solution was added, and static precipitation was performed for 12 hours;
1.4 Filter the solution, measure 479.3 ml of the filtrate volume, add 479.3 ml of saturated ammonium sulfate solution, and let it stand for precipitation for 12 hours;
1.5 Washing magnesium: discard the supernatant, filter the precipitate in vacuum, add saturated magnesium sulfate solution to the filter cake, let stand for 1 minute, and filter with suction. When the filtrate begins to flow out, pour the remaining magnesium sulfate solution on the funnel. Go, take out the filter cake and weigh 93.3g, to be activated;
2. Activation
Dissolve the filter cake in 373.2ml of 0.005mol/L hydrochloric acid solution, add 186.6ml of 1mol/L calcium chloride solution and 466.5ml of pH8.0 boric acid buffer, then add 746.4ml of purified water, adjust the pH to 7.4, and finally add 933mg Crystallized trypsin with high activity was activated, and the solution was left standing at 5-10°C for 72 hours for activation, and the pH value was measured at 7.4 the next day;
3. Calcium removal
The activated solution was adjusted to pH 3.1 with 2.5mol/L sulfuric acid solution, the solution volume was 1873ml, then 455.2g of solid ammonium sulfate was added, stirred to dissolve, stored at 0-10°C for 48 hours to precipitate calcium sulfate, filtered, and added to the filtrate. Solid ammonium sulfate 369.0g, left standing at 0~10 ℃ for 12 hours, filtered to obtain a filter cake weight of 45.6g;
4. Crystallization
Add 68.4 ml of boric acid buffer at pH 9.0 to the filter cake, adjust the pH to 8.0 with 2.5 mol/L sulfuric acid solution, stir evenly, then put the solution in a dialysis bag, immerse it in the external permeate at 5-10 °C, and crystallize for 5 days. Shake once every 2 hours;
5. Dialysis
Take out the crystal solution and filter to obtain 28.7g of crystals, add 43.1ml of purified water to dissolve, and adjust the pH to 3.0 with 2.5mol/L sulfuric acid; put the solution in a dialysis bag, and then hang it in a dialysis tank for 3 days to remove salt impurities. Change the water in the dialysis tank every 12 hours, and control the temperature at 5-10°C;
6. Lyophilization
Take out the dialysate, add a small amount of diatomaceous earth, and filter with a Buchner funnel to obtain the filtrate, adjust the pH to 6.0 with 5mol/L sodium hydroxide solution, and freeze-dry it in a freeze dryer to obtain 6.2g of crystalline trypsin finished product. Its active titer is 2600iu/mg;

About Us

The production base is located in Zhangqiu chemical industry park and Tai’an high-tech chemical industry park. laboratory and workshop in strict accordance with the GMP standard and the product fit national ISO9001 and ISO2000 standards.

“Zhishang” products are exported to Europe, North and South America, the Middle East, Asia Pacific and Africa area, so as to establish a long-term and stable cooperation relationship with customer in the world.

Company Info
  • Business Type: Manufacturer
  • Product Range: Additive , Chemical Auxiliary & Catalyst , Organic Chemicals
  • Products/Service: Organic Intermediate,Inorganic Chemistry, APIs, Dyestuffs And Pigments, Fragrance And Spices, Food Additives
  • Total Employees: 51~100
  • Capital (Million US $): 10000000RMB
  • Year Established: 2016
Production Capacity
  • No. of Production Lines : 8
  • No. of QC Staff : 5 -10 People
  • OEM Services Provided : yes
  • Factory Size (Sq.meters) : 3,000-5,000 square meters
  • Certificate: ISO9001 , CE , GMP , API , MSDS
  • Factory Location : Diao Town Industry Park, Zhangqiu City, Jinan City, Shandong Province, China.

Service

Pre-Sales Service

* Prompt reply and 24 hours online, professional team to provide best price and high quality product.

* Sample testing support.

* Every batch of products will be tested to ensureits quality.

*The packing also can be according the customers` requirment.

*Any inquiries will be replied within 24 hours.

*we provide Commerical Invoice, Packing List, Bill of loading, COA , Health certificate and Origin certificate. If your markets have any special requirements, let us know.

 

After-Sales Service

*The fact of logistics information monitoring.

* Any questions about the product can be consulted at any time.

*Product has any problem can return.

FAQ

Do you accept sample order?

We will make samples before mass production, and after sample approved, we’ll begin mass production. Doing 100% inspection during production, then do random inspection before packing.

 

HOW TO CONFIRM THE PRODUCT QUALITY BEFORE PLACING ORDERS?

You can get free samples for some products,you only need to pay the shipping cost or arrange a courier to us and take the samples. You can send us your product specifications and requests,we will manufacture the products according to your requests.

What’s your MOQ?

Our MOQ is 1kg. But usually we accept less quantity such as 100g on the condition that sample charge is 100% paid.

Do you supply product report?

Yes. We’ll give you product analysis report before shipping.

  Is there a discount?

Different quantity has different discount.

Shipping

1. ≤50kg, Express delivery recommended, usually called as DDU service;

2. ≤500kg, Air shipping recommended, usually called as FOB, CFR, or CIF service;

3. >500kg, sea shipping recommended, usually called as FOB, CFR, or CIF service;

4. For high value products, please select air shipping and express delivery for safe.

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