Light belongs to visible light, next to the ultraviolet band. Although it is not as good as ultraviolet rays, blue light has a wavelength band between 400 and 500nm, which is the highest energy band in visible light.
Blue light can penetrate deeper into the skin causing oxidative damage to cellular DNA.
Paris famous dermatologist Marie-Estler Lu said: Blue light can cause melanin pigmentation for up to 3 months, especially in the epidermal granular layer and deeper skin layer.
Troxerutin is extracted from Sophora japonica, which is a natural plant-derived component. It is one of the flavonoid rutin derivatives. Troxerutin has better water solubility and better efficacy than rutin. Troxerutin has a strong absorption effect on blue light, and at the same time has a good anti-oxidation effect, reducing various damages to the skin caused by free radicals, and can achieve the effect of anti-blue light.
Troxerutin Safety – HaCaT Cytotoxicity HaCaT cells (4×104 cells) were seeded into 96-well plates and pre-incubated with indicated concentrations of Troxerutin for 4, 8 and 12 hours. After pretreatment, HaCaT cells were exposed to 50 mJ/cm², and cell viability was measured by the water-soluble tetrazolium salt (WST-1) assay. Results are presented in triplicate and expressed as mean ± SE of percent optical density (OD) of the control. Asterisks indicate significant differences compared to HaCaT cells treated with UVB alone (*p<0.05)
1. Troxerutin scavenges free radicals and protects cells from oxidative stress-induced damage
Studies have found that after cells are irradiated by blue light, active oxygen free radicals are generated in mitochondria, and these highly active oxygen free radicals can damage mitochondria and DNA. Troxerutin absorbs blue light, reduces the damage of blue light to cells, and achieves the effect of preventing blue light.
2. Troxerutin reduces the damage of blue light to the skin by absorbing blue light
3. Troxerutin anti-blue light-reduces the damage of blue light to the skin by scavenging free radicals
Studies have found that after cells are irradiated by blue light, active oxygen free radicals are generated in the mitochondria, and these highly active oxygen free radicals can
Damage mitochondria and DNA. Troxerutin reduces the generation of oxygen free radicals through anti-oxidation, and achieves the effect of preventing blue light.
Effect of troxerutin on scavenging oxygen free radicalsComparison of troxerutin and VC on scavenging oxygen free radicals
Troxerutin UV protection – protecting cells from UVB-mediated growth arrest
Troxerutin prevented UVB-mediated growth arrest in a dose-dependent manner.
Troxerutin (5 μM) effectively shields cells from UVB-mediated growth apprehension. Especially, pretreatment with 5 μM troxerutin for 8 h enhanced cell feasibility by 20.27% compared to unattended HaCaT cells exposed to UVB (Fig. 2).
Overall, pretreatment with troxerutin safeguarded cells from UVB-mediated growth apprehension.
Troxerutin safeguards versus UV rays—- changes miRNA expression in UVB HaCaT cells
Troxerutin alters miRNA expression accounts in UVB-exposed HaCaT cells As explained in the Introduction, miRNAs play key functions in UVB responses with details binding to target genes.
We utilized a miRNA genetics microarray consisting of 1,368 probes representing 1,205 human miRNAs. Overall RNA labeled with Cy3 (an environment-friendly fluorescent dye) was removed from HaCaT cells treated with or without troxerutin and afterwards revealed to UVB radiation. Each Cy3-labeled example was hybridized to the miRNA microarray. Utilizing evaluation software (GeneSpring GX version 11.5; Agilent), we initially stabilized the data for every sample making use of worldwide normalization.
In previous researches, troxerutin-induced DNA repair work improved its protection versus gamma rays. Troxerutin enhanced DNA repair work activity in HaCaT cells.
Moreover, using miRNA microarray evaluation and also bioinformatics tools, we determined teroxetine-induced miRNAs and also found web links between teroxetine-induced miRNAs, along with anti-apoptosis, improved migration, and DNA repair work effects. In HaCaT cells exposed to UVB, troxerutin changed the expression of 68 miRNAs, which were upregulated or downregulated ≥ 2-fold.
