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Tris(2-carboxyethyl)Phosphine Hydrochloride CAS 51805-45-9

Molecular Formula: C9H16ClO6P

Formula Weight: 286.65

ZSpharmac: Tris(2-carboxyethyl)Phosphine Hydrochloride Supplement

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Product Name: Tris(2-carboxyethyl)Phosphine Hydrochloride
CAS No: 51805-45-9
Purity: 99%

Basic Info

Product Name:Tris(2-carboxyethyl)Phosphine Hydrochloride
Other Names:Tris(2-Carboxyethyl)phosphine Hydrochloride
CAS:51805-45-9
Place of Origin:Shandong, China
Brand Name:ZSpharmac
Type:Organic Intermediate
Appearance:White Powder
EINECS No.:629-759-1
Storage:2-8°C
Provide:Tris(2-carboxyethyl)Phosphine Hydrochloride MSDS;
Tris(2-carboxyethyl)Phosphine Hydrochloride COA

What is Tris(2-carboxyethyl)Phosphine Hydrochloride?

Tris(2-carboxyethyl)phosphine hydrochloride (TCEP hydrochloride) is an unique decreasing and deprotecting representative for thiolated DNA. Thiolated DNA incurable sulfur atoms often tend to create dimers in solution, specifically in the presence of oxygen. This dimerization greatly reduces the performance of some combining reaction experiments (such as DNA immobilization in biosensors); while tris(2-carboxyethyl)phosphine hydrochloride (TCEP hydrochloride) is included in the DNA solution ), which can be eliminated after a duration of response to reduce DNA dimerization. Tris (2-carboxyethyl) phosphine hydrochloride (TCEP hydrochloride) is also commonly made use of for the reduction of disulfide bonds in healthy proteins, which can be made use of to prevent intramolecular or intramolecular healthy protein formation between cysteines in healthy proteins. Intermolecular disulfide bonds. Tris(2-carboxyethyl)phosphine hydrochloride (TCEP hydrochloride) is more stable than the standard minimizing as well as deprotecting agents mercaptoethanol or dithiothreitol (DTT) for thiolated DNA, Difficult to be oxidized by air, with no irritating odor.

Tris(2-carboxyethyl)Phosphine Hydrochloride Properties:

Melting point 177 °C
density 1.041 g/mL at 25 °C
refractive index n20/D 1.367
storage temp. 2-8°C
solubility H2O: 50 mg/mL
pka7.66(at 25℃)
form powder
color white
PH7.0(aqueous solution; pH was adjusted with ammonium hydroxide)
Water Solubility Soluble in water and methanol.

 

Tris (2-carbonylethyl) Phosphate Hydrochloride application

Application 1.
CN201910350963.7 discloses a proteome sample processing kit, processing method and application. The kit includes: tris(2-carbonylethyl)phosphonium hydrochloride, 2,2-dichloroacetamide, sodium deoxycholate, and buffer. Applying the technical scheme of the present invention, before the proteome sample is processed, the tris(2-carbonylethyl) phosphate hydrochloride, 2,2-dichloroacetamide, sodium deoxycholate and buffer in the kit of the present invention are mixed together. into a treatment solution in which cell/tissue samples are lysed and intracellular total protein denatured by sodium deoxycholate, tris(2-carbonylethyl) phosphate hydrochloride as reducing agent, 2,2-dichloroacetamide As an alkylating reagent, proteomic samples can be lysed and reductively alkylated within minutes in the processing solution. That is to say, in the present invention, the cleavage, reduction and alkylation are completed in one step, which makes the proteome sample processing process simple and saves time.
Application 2.
CN201811195644.5 reports a low eutectic solvent monolithic column enzyme reactor and a preparation method thereof. Preparation steps: add free radical initiator azobisisobutyronitrile into deep eutectic solvent/ionic liquid, after ultrasonic dispersion, add functional monomer methacrylic acid, comonomer butyl methacrylate, crosslinking agent dimethyl methacrylate Ethylene glycol methacrylate, dissolved by ultrasonic, injected into the capillary, thermally induced to synthesize monolithic column, and tris(2-carbonylethyl) phosphate hydrochloride (TCEP-HCl) reagent was used to reduce the disulfide bond in trypsin to sulfhydryl ,
adding ammonium persulfate and N,N’-methylenebisacrylamide to prepare an enzyme derivative solution, reacting with the monolithic column at room temperature and immobilizing it on the surface of the monolithic column for enzymatic protein hydrolysis.
The monolithic column has a stable structure and has a low impact on enzyme activity, and can be used as a new type of reusable enzymatic reactor for enzymolysis of protein solution.

Preparation of Tris(2-carboxyethyl)Phosphine Hydrochloride

1. Preparation of compound trimethyl 3,3′,3″- phosphine triyl tripropionate Under the problem of mechanical stirring, 80% tetrahydroxymethylphosphorus chloride liquid solution (71.2 g, 0.30 mol) and ethanol (75ml) were contributed to a 250ml response flask, cooled with ice water as well as protected by nitrogen, and after that included with hydroxide Potassium (16.8 g, 0.30 mol) was dissolved in a service of 23ml of ice water, as well as the temperature rose slightly; then ethyl acrylate (90g, 0.9 mol) was included dropwise, and also the temperature level was managed at 35-40 ° C, a big quantity of strong was created, as well as the temperature level was kept stirring after going down. 4 hrs. Put 300 ml of ice water into the reaction option, filter with suction after mixing, wash the filter cake with ice water, as well as completely dry with suction to acquire 88 g of white solid (that is, compound trimethyl 3,3′,3″- phosphinetriyltripropylidene) acid ester), the return was 86.6%, which was made use of for the next reaction. The nuclear magnetic data of the gotten item were as complies with: 1HNMR (CDCl3, 400MHz): δ= 1.27( t, 9H), 1.75( t, 6H), 2.45( dt, 6H), 4.15( q, 6H). 2. Preparation of substance tris( 2-carboxyethyl) phosphine hydrochloride (TCEP hydrochloride). Under the problem of mechanical stirring, the trimethyl 3,3′,3″- phosphine triyl tripropionate (88 g, 0.26 mol) prepared by the above response and the focused 12 mol/L molar concentration were contributed to the 250 ml response flask. Hydrochloric acid (120ml, 1.44 mol) was warmed to 80 ° C with mixing, the solid was progressively liquified, the reaction was proceeded for 3 hours, mixed and cooled down with ice water to separate out a white strong, suction filtered, as well as the filter cake was recrystallized with distilled water to get white crystals, particularly 3. (2-carboxyethyl) phosphine hydrochloride, stove dried out, considered 57.2 g, yield 76.9%. mp174-176 ℃. Nuclear magnetic data are as follows: 1HNMR (D2O, 400MHz): δ= 2.57( dt,6 H), 2.88( dt, 6H).

Tris(2-carbonylethyl)Phosphonium Hydrochloride Properties

1. Solubility
The molecular weight of TCEP hydrochloride is 286.65, the solubility in water is 310g/L, and the corresponding molar concentration is 1.08mol/L. Due to its hydrochloride structure, it has good solubility in aqueous solutions of various pH values, and it is very convenient to prepare aqueous solutions of various concentrations. The solubility of TCEP hydrochloride in conventional organic solvents such as methanol and ethanol is very small.
2. Solution stability
TCEP hydrochloride is relatively stable in acidic and alkaline aqueous solutions. After TCEP hydrochloride is directly dissolved in water, the pH value of the aqueous solution is about 2.5. Studies have shown that: when TCEP hydrochloric acid is prepared into 100mMol/L hydrochloric acid, sodium hydroxide or other types of buffer solutions, after the solution is stored at room temperature for 24 hours (see Table 1 for the list of buffer solutions), the active ingredients are almost not reduced. Tested after a few weeks, only about 20% of the TCEP hydrochloride was oxidized to failure.
3. Effectively restore the PH value range
Compared with DTT, TCEP hydrochloride can reduce disulfide bonds in a wider pH range [1]. Experiments show that it only takes 30 seconds to completely reduce dithiodipyridine in the range of PH=1.5-9.0. If pH > 9.0, only 50% of the dithiodipyridine is reduced. Compared with DTT, TCEP hydrochloride is more reducible when the solution pH is less than 8.0, and it can even reduce DTT in its oxidized state.
4. Effective reducing concentration
In general, using a relative excess of TCEP hydrochloride at a concentration of 5-50 mMol/L can completely reduce the disulfide bonds in polypeptides or proteins at room temperature in a few minutes. The Han test showed that using 30uMol/L TCEP hydrochloride, in the range of PH=1.5-8.5, dithiodipyridine can be completely reduced in about 40 minutes. If only an equivalent amount of TCEP hydrochloride is used, complete reduction will take longer. Mery reports that the approximate time for complete reduction of disulfide bonds using a 1:1 equivalent of TCEP hydrochloride is 1 hour.
5. Scope of use
(1) Because TCEP hydrochloric acid does not contain a sulfur structure, its reduction reaction solution does not require additional purification, and can be directly used in solutions containing maleimide or cross-linking reagents. In general, the concentration of TCEP hydrochloride is less than 10-20mMol/L, which can be directly used in chemical reactions containing maleimide.
(2) 50mMol/L TCEP hydrochloride solution can be used as a substitute for DTT or 2-mercaptoethanol, but TCEP hydrochloride needs to be in solution to function, so it is not suitable for isoelectric focusing electrophoresis (IEF).
(3) When it comes to antibody labeling by reducing disulfide bonds in the immunoglobulin hinge region, it is necessary to reduce the heavy chain and light chain at the same time. 2-mercaptoethylamine is generally used for this particular reduction operation. Blauenstein confirmed that TCEP hydrochloride can also be used for the above special reduction operation. Add 3.8-4.0mMol/L TCEP hydrochloride solution to 10mg/ml immunoglobulin in 0.1mol/L phosphate buffer solution, the pH of the buffer solution is about 4.6-7.5, at room temperature for 20-30 minutes. Sulfur bond reduction, by-products of TCEP hydrochloride reduction can be removed by passing through a desalting column.

Company Profile and Corporate Culture

Company Profile:

ZhiShang Chemical is owned by ZhiShang Group is a professional new-type chemicals enterprise combined into research and development, production and sales .

The company’s competitive product is pharmaceutical raw materials and intermediates (especially carbohydrate derivatives Series), In recent years, the company has made a major breakthrough in food and feed additives, plant extraction, industrial chemicals industry .

The company insists on the spirit of “sincere management, strict quality control, customer as god” , get consistent high praise from customers at home and abroad.

Corporate Culture:

Our Mission
Help China Chemicals to benefit the happiness of human life
Our Vission
Become the most trusted chemical supplier in the world
Our Business Philosophy
Striver – oriented, enrich employees, customer first, deep service, seek development
Our Values
Be prepared for danger in times of peace, forge ahead actively, unity and cooperation, and be brave to fight

About Us

The production base is located in Zhangqiu chemical industry park and Tai’an high-tech chemical industry park. laboratory and workshop in strict accordance with the GMP standard and the product fit national ISO9001 and ISO2000 standards.

“Zhishang” products are exported to Europe, North and South America, the Middle East, Asia Pacific and Africa area, so as to establish a long-term and stable cooperation relationship with customer in the world.

Company Info
  • Business Type: Manufacturer
  • Product Range: Additive , Chemical Auxiliary & Catalyst , Organic Chemicals
  • Products/Service: Organic Intermediate,Inorganic Chemistry, APIs, Dyestuffs And Pigments, Fragrance And Spices, Food Additives
  • Total Employees: 51~100
  • Capital (Million US $): 10000000RMB
  • Year Established: 2016
Production Capacity
  • No. of Production Lines : 8
  • No. of QC Staff : 5 -10 People
  • OEM Services Provided : yes
  • Factory Size (Sq.meters) : 3,000-5,000 square meters
  • Certificate: ISO9001 , CE , GMP , API , MSDS
  • Factory Location : Diao Town Industry Park, Zhangqiu City, Jinan City, Shandong Province, China.

Service

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*we provide Commerical Invoice, Packing List, Bill of loading, COA , Health certificate and Origin certificate. If your markets have any special requirements, let us know.

 

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FAQ

Do you accept sample order?

We will make samples before mass production, and after sample approved, we’ll begin mass production. Doing 100% inspection during production, then do random inspection before packing.

 

HOW TO CONFIRM THE PRODUCT QUALITY BEFORE PLACING ORDERS?

You can get free samples for some products,you only need to pay the shipping cost or arrange a courier to us and take the samples. You can send us your product specifications and requests,we will manufacture the products according to your requests.

What’s your MOQ?

Our MOQ is 1kg. But usually we accept less quantity such as 100g on the condition that sample charge is 100% paid.

Do you supply product report?

Yes. We’ll give you product analysis report before shipping.

  Is there a discount?

Different quantity has different discount.

Shipping

1. ≤50kg, Express delivery recommended, usually called as DDU service;

2. ≤500kg, Air shipping recommended, usually called as FOB, CFR, or CIF service;

3. >500kg, sea shipping recommended, usually called as FOB, CFR, or CIF service;

4. For high value products, please select air shipping and express delivery for safe.

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