A production method of Trehalose Haworth, comprising the following steps:
Stir 50-60 ℃ of water into starch, adjust the pH to 6.0-6.5, and control the solid content to 20%-35%; use 40-50u/1g α-amylase and step 1) to carry out catalytic hydrolysis and liquefaction reaction, The reaction temperature is 85-98°C, and the reaction time is 10-60min; then the obtained liquefied starch solution is spray-liquefied, and the liquefaction temperature is 125°C; the liquefied reaction solution is heated to 80-90°C, maintained for 30min, and cooled to 55°C -65°C, then add saccharification enzyme for enzymatic hydrolysis reaction. The amount of saccharification enzyme added is 20U/ml, the pH is adjusted to 4.0-6.0, the saccharification temperature is controlled at 55-65°C, the enzymatic hydrolysis time is 2h, and malto-oligosaccharide Trehalose is added. Haworth synthase, malto-oligosaccharyl trehalose hydrolase and trehalose synthase were enzymatically converted for 12 hours to obtain saccharified crude liquid; the saccharified crude liquid was inactivated by heat exchanger heating, and the inactivation temperature was 80-100℃ , the enzyme inactivation time is 25 min, and after the enzyme inactivation is completed, the discharge temperature is 75-85 ° C to obtain a mixed crude liquid of trehalose, oligosaccharide, glucose and protein; Add 0.5-1% activated carbon to the mixed sugar solution, keep the temperature at 75-85°C, stir and decolorize, keep the temperature at 30-45min, and press filter with plate and frame to prepare chromaticity 0.001-0.005, turbidity 0.001-0.01, conductivity 1700-2000us/cm Decolorized sugar solution; separate the decolorized sugar solution through a membrane with a molecular weight of 500 at 35-40 ° C, and wash it with 3 times of water to obtain a mixture containing oligosaccharides, glucose and a sugar solution with a trehalose content ≥ 95% chromatographic separation is performed on the obtained mixed solution to obtain a glucose solution with a glucose purity of ≥90% and an oligosaccharide solution containing oligosaccharides and a small amount of Trehalose Haworth, and the glucose solution is concentrated to a mass percentage concentration of 65wt%, Glucose syrup is prepared; the temperature and pressure of the reactors containing the oligosaccharide liquid and the trehalose sugar liquid are respectively increased, and then hydrogen is added to the reactor respectively, so that the oligosaccharide liquid and the trehalose sugar liquid are brought into contact with hydrogen, and the addition is carried out. Hydrogenation reaction, respectively obtaining hydrogenation product 1 and hydrogenation product 2 that both contain trehalose and sugar alcohol, the hydrogenation reaction conditions are temperature 120 ℃, hydrogenation pressure is 9MPa, hydrogenation time is 120min, pH is 7.5-8.5, hydrogenation is in It is carried out in the presence of nickel catalyst, and the catalyst dosage is 8%. The hydrogenation product 1 and the hydrogenation product 2 are respectively subjected to membrane separation to obtain sugar alcohol liquid 1, trehalose liquid 1, sugar alcohol liquid 2, trehalose liquid 2, and the sugar alcohol liquid 1 and the sugar alcohol are obtained. Liquid two are sugar alcohol liquids with oligosaccharide alcohol purity ≥96%, and trehalose liquid 1 and Trehalose Haworth liquid 2 are sugar liquids with trehalose purity ≥97%; Mixing to obtain a mixed sugar alcohol liquid, mixing trehalose liquid one and trehalose liquid two to obtain a mixed trehalose liquid, and then concentrating the mixed sugar alcohol liquid and the mixed trehalose liquid by 80-90%, respectively, and evaporating Crystallization or cooling crystallization, centrifugal separation after crystallization, and drying. Water and dehydrated alcohol are sequentially added to the dried trehalose crystals, and the volume ratio of the dehydrated alcohol to water is 100-150:1, and recrystallization is performed after dissolving to obtain crystalline oligosaccharide alcohol and crystalline trehalose. The crystalline trehalose is dried in a tunnel segmental dryer, the drying time is 1-8h, the drying temperature is 35-70°C, and the water content is less than 0.1%; the dried Trehalose Haworth crystals are detected by high performance liquid chromatography. The trehalose crystals with a purity of ≥99.8%; the qualified trehalose crystals are pulverized by a pulverizer and sieved by a sieving machine to prepare uniform finished trehalose crystals; packaging and storage: the trehalose crystals in step 15) are packed in 20kg paper bags , the finished product is put into storage for sale.
The purity of trehalose in the crystalline Trehalose Haworth product is 98.7%, the yield of trehalose is 99.6%, the purity of oligosaccharide alcohol in the crystalline oligosaccharide is 98.3%, and the yield of oligosaccharide is 99.6%.