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Tca Trichloroacetic Acid CAS 76-03-9

Molecular Formula: C2HCl3O2

Formula Weight: 163.39

ZSpharmac: Tca Trichloroacetic Acid Supplement

Product Name: Tca Trichloroacetic Acid
CAS No: 76-03-9
Purity: 99%

Basic Info

Product Name:Tca Trichloroacetic Acid
Other Names:Trichloroacetic Acid, 99%, ACS Reagent
Place of Origin:Shandong, China
Brand Name:ZSpharmac
Type:Pharmaceutical Intermediates
Appearance:White Solid
EINECS No.:200-927-2
Storage:Store at +15°C to +25°C.
Provide:Tca Trichloroacetic Acid MSDS;
Tca Trichloroacetic Acid COA

What is Tca Trichloroacetic Acid?

Tca Trichloroacetic Acid is referred to as TCA. Colorless or white orthorhombic crystal. A strong deliquescence. There is a slight special pungent odor. There is strong corrosiveness. The relative molecular mass is 163.40. The relative density is 1.6298 (61°C). Melting point 57 ~ 58 ℃. Boiling point 197.5°C, 141~142°C (3.333×103Pa). Combustion heat 3.0kJ. Refractive index 1.4603 (61°C). Vapor pressure 0.133×103Pa (51℃). Easily soluble in water, the aqueous solution is acidic (0.1mol aqueous solution pH 1.2), soluble in ethanol, ether, solubility at 25°C: water 1306, methanol 2143, ether 617; the aqueous solution below 30% is slowly decomposed into chloroform, hydrogen chloride, Carbon dioxide, carbon monoxide, etc.; hydrolyzed to chloroform and carbon dioxide in dilute alkaline solution; hydrolyzed to formic acid in concentrated alkaline solution. It is decomposed into chloroform and carbonate with strong alkali. Its aqueous solution is strongly acidic and is mainly used in organic synthesis (such as drugs), and can be used as a decalcifying agent and a fixative when using a microscope. This product is strongly corrosive. Trichloroacetic acid is classified as a highly toxic drug, so be careful when using it. The oral toxicity of mice is 3300mg/kg, and the oral LD503320mg/kg of rats can strongly stimulate the skin and tissue. Burns when attached to skin. Trichloroacetic acid is a strong organic acid with a dissociation constant K=3×10-2. Active chemical properties, its sodium salt is easily decarboxylated into chloroform, and when LiAlH4 is encountered, it will be reduced to alcohol, and it will undergo a halogen replacement reaction with KBr:

三氯乙酸 化学反应式
Trichloroacetic acid can be obtained by direct chlorination of acetic acid or by oxidation of trichloroacetaldehyde by nitric acid: CCl3CHO+2HNO3→CCl3COOH+2NO2+H2O.

Tca Trichloroacetic Acid Properties:

Melting point 54-58 °C (lit.)
Boiling point 196 °C (lit.)
density 1.62 g/mL at 25 °C (lit.)
vapor density <1 (vs air)
vapor pressure 1 mm Hg ( 51 °C)
refractive index n20/D 1.62(lit.)
Fp 196°C
storage temp. Store at +15°C to +25°C.
solubility H2O: 0.5 M at 20 °C, clear, colorless
pka0.7(at 25℃)
form Solid
color White
PH<1.0 (25℃, 0.5M in H2O)
Water Solubility 120 g/100 mL (20 ºC)


Tca Trichloroacetic Acid Uses

  1. Tca Trichloroacetic Acid is used for the determination of fluoride, protein precipitant, and ferric chloride as a reagent for bile pigments and a fixative for microscopic analysis. This product is a wart remover and astringent in medicine. Sodium trichloroacetate is a selective herbicide. It is mainly used as an extractant for biochemical drugs, such as adenosine triphosphate, cytochrome C and placental ester polysaccharide. Raw materials for pesticides. Traditionally used to precipitate proteins. Used as a decalcifier and as a fixative for microscopy samples. When trichloroacetic acid is heated with alkali solution, it is decomposed into chloroform and alkali carbonate.
  2. Tca Trichloroacetic Acid is used as adenosine triphosphate, cytochrome C and placental lipopolysaccharide extractant and protein precipitant, as well as pesticide herbicide and organic synthesis raw material.
  3. Tca Trichloroacetic Acid is used in medicine as a wart remover and astringent.

Principle of Tca Trichloroacetic Acid Precipitation of Proteins

① Form insoluble salts with proteins under acidic conditions;
② As a protein denaturant, it changes the protein conformation, exposes more hydrophobic groups, and causes it to aggregate and precipitate;
③ As the molecular weight of the protein increases, its structure becomes more complex and dense. TCA may penetrate into the molecule and make it difficult to be completely removed. When the sample is heated before electrophoresis, the protein structure may undergo acid hydrolysis and form fragments. , and this effect becomes more and more obvious with the extension of time;
④ The electrophoresis spectrum shows that the monomer spectrum of BSA and HSA has obvious broadening phenomenon, which may be due to the binding of TCA, which makes the binding amount of SDS and protein deviate, resulting in the inhomogeneity of the charge on the protein, resulting in migration. rate inconsistency.

Tca Trichloroacetic Acid Precipitation Method

1) Add 1/4 volume of TCA+DOC to the protein fraction (in a 1.5-ml polypropylene microcentrifuge tube) to a final concentration of TCA of 20% (w/v). Shake to mix.
2) Incubate on ice for 20-30 min.
3) Microcentrifuge, centrifuge at room temperature for 15 min. The precipitate, if visible, was a viscous, yellowish-brown gum. Aspirate the supernatant with a fine Pasteur pipette. Strive to remove as much supernatant as possible. If a 100ul sample is taken for precipitation, the precipitate will be visible.
4) Add 3 times the volume (original sample volume of acetone (room temperature). The sample is allowed to stand at room temperature for about 10 min, so that TCA+DOC is dissolved in acetone.
5) Centrifuge at room temperature for 15 min. At the time, the size and physical properties of the sediment resembled that of a bit of dust. About 10ug or more of protein is visible. Sometimes, white precipitates of salts (such as KCl, etc.) are obtained. Remove the supernatant with a very fine Pasteur pipette. The pellet was dried on ice for 10 min (the lid of the 1.5-ml centrifuge tube was opened). Dried pellets can be stored at -20°C for extended periods (>1 month).
6) Dissolve the pellet with 2-mercaptoethanol-containing sample buffer (lx) (6ul for a standard mini-plate gel apparatus). If there is still a small amount of TCA residue, the bromophenol blue will turn yellow. This does not matter because there is enough buffer capacity to neutralize traces of TCA during electrophoresis. If there is residual KCl in the precipitate, a flocculent white precipitate (potassium dodecyl sulfate) will form when sample buffer is added to the precipitated protein. If potassium dodecyl sulfate precipitates, it is difficult to apply the sample to an SDS gel, but once the sample is applied to the gel, electrophoresis will run well.
7) If a molecular weight standard reference is required, the protein molecular weight (MW) standard can be prepared as a stock solution of 0.5 mg total protein per ml in lxSDS sample buffer without 2-mercaptoethanol. Divide into small aliquots (50ul) and store at -20°C. For silver staining, take 2ul molecular weight standard + 4ul sample buffer containing 2-mercaptoethanol to a final volume of 6ul.

Production Method of Tca Trichloroacetic Acid

1. After chlorination of acetic acid, the mother liquor of chloroacetic acid is used as the raw material, the sulfur powder is used as the catalyst, and the chlorination is continued under the condition of 90-100 ℃, and then recrystallized. 2. Trichloroacetaldehyde method is obtained by co-melting oxidation of trichloroacetaldehyde and fuming nitric acid. Trichloroacetic acid can be obtained by oxidizing trichloroacetaldehyde with nitric acid or potassium permanganate, and directly chlorinating acetic acid under the catalysis of iodine or phosphorus trichloride and light irradiation.
The preparation methods are mainly as follows. (1) Use trichloroacetaldehyde to oxidize with dilute nitric acid, and the reaction temperature is 80~100℃. CCl3CHO+2HNO3→Cl3CCOOH+NO2↑+H2O(2) is obtained by further deep chlorination of monochloroacetic acid mother liquor with sulfur powder as a catalyst and crystallization. ClCH2COOH+Cl2[S]→Cl3CCOOH+2HChemicalbookCl(3) Using tetrachloroethylene as raw material, it is oxidized with oxygen at 120 ℃ under ultraviolet irradiation or using tetrachloroethylene as raw material, titanium as catalyst, and hydrolyzed at 40 ~ 120 ℃, Trichloroacetic acid can be obtained. Cl2CH=CHCl2+O2→Cl3CCOOH+HClCl2CH=CHCl2+2H2O→Cl3CCOOH+HCl↑+2H2↑

The Dangers and First Aid Measures of Tca Trichloroacetic Acid

Inhalation of the dust of this product has an irritating effect on the respiratory tract and can cause cough, chest pain and central nervous system depression. Direct eye contact can cause serious damage, including blindness. Skin contact can cause chemical burns. In the past, TCA burns were more common because they were often used for gum burns. It is a very corrosive drug and can cause severe burns if used incorrectly. Clinically, the surface may be whitish, followed by tissue necrosis, and inflammation, rot or ulcer may appear under the white necrotic tissue. Oral burns to the mouth and digestive tract, severe abdominal pain, vomiting and collapse.
When a leak occurs, do not directly touch the leak, sprinkle soda ash on the ground, then rinse with plenty of water, and put the diluted washing water into the waste water system. If there is a large amount of leakage, it should be discarded after collection and recycling or harmless disposal.

Protective measures: Respiratory protection: When the concentration in the air exceeds the standard, you should wear a gas mask. Wear self-contained breathing apparatus when necessary.
Eye Protection: Wear chemical safety goggles.
Protective clothing: Wear work clothes (made of anti-corrosion materials).
Hand Protection: Wear rubber gloves.
Others: After work, shower and change. Pay attention to personal hygiene.
Skin Contact: Remove contaminated clothing and flush immediately with water for at least 15 minutes. Rinse with plenty of water, or with 2% soda water. If burns occur, seek medical attention.
Eye Call: Immediately lift eyelids and flush with running water or saline for at least 15 mins. seek medical attention.
Inhalation: Quickly leave the scene to fresh air. Keep the airway open. Provide artificial respiration if necessary. seek medical attention.
Ingestion: If swallowed by mistake, rinse mouth immediately and drink milk or egg white. seek medical attention.

Tca Trichloroacetic Acid Curing

(1) Warehousing acceptance: check whether the outer packaging is exposed to rain, water immersion, pollution or rupture. Whether the packaging seal is tight. Whether the item deliquescent deepens.
(2) Stacking thatch pads: The bottom of the cargo should be padded with a height of 15-30cm, and the height of the stack should not exceed 3m.
(3) In-store inspection: During the storage period, the cargo stack and the environment should be inspected once every day after going to get off work and before work, and a comprehensive inspection should be carried out on a regular basis every quarter.
(4) Temperature and humidity management: The warehouse should be ventilated frequently to keep it dry. In the rainy season, when the relative humidity in the warehouse reaches 80%, a hygroscopic machine or lime blocks can be used to absorb moisture.
(5) Safe operation: Wear work clothes and gloves when handling, and handle with care when loading and unloading stacks.
(6) Storage period: 1 year.

Company Profile and Corporate Culture

Company Profile:

ZhiShang Chemical is owned by ZhiShang Group is a professional new-type chemicals enterprise combined into research and development, production and sales .

The company’s competitive product is pharmaceutical raw materials and intermediates (especially carbohydrate derivatives Series), In recent years, the company has made a major breakthrough in food and feed additives, plant extraction, industrial chemicals industry .

The company insists on the spirit of “sincere management, strict quality control, customer as god” , get consistent high praise from customers at home and abroad.

Corporate Culture:

our mission
Help China Chemicals to benefit the happiness of human life
our vision
Become the most trusted chemical supplier in the world
our business philosophy
Striver – oriented, enrich employees, customer first, deep service, seek development
our values
Be prepared for danger in times of peace, forge ahead actively, unity and cooperation, and be brave to fight

About Us

The production base is located in Zhangqiu chemical industry park and Tai’an high-tech chemical industry park. laboratory and workshop in strict accordance with the GMP standard and the product fit national ISO9001 and ISO2000 standards.

“Zhishang” products are exported to Europe, North and South America, the Middle East, Asia Pacific and Africa area, so as to establish a long-term and stable cooperation relationship with customer in the world.

Company Info
  • Business Type: Manufacturer
  • Product Range: Additive , Chemical Auxiliary & Catalyst , Organic Chemicals
  • Products/Service: Organic Intermediate,Inorganic Chemistry, APIs, Dyestuffs And Pigments, Fragrance And Spices, Food Additives
  • Total Employees: 51~100
  • Capital (Million US $): 10000000RMB
  • Year Established: 2016
Production Capacity
  • No. of Production Lines : 8
  • No. of QC Staff : 5 -10 People
  • OEM Services Provided : yes
  • Factory Size (Sq.meters) : 3,000-5,000 square meters
  • Certificate: ISO9001 , CE , GMP , API , MSDS
  • Factory Location : Diao Town Industry Park, Zhangqiu City, Jinan City, Shandong Province, China.


Pre-Sales Service

* Prompt reply and 24 hours online, professional team to provide best price and high quality product.

* Sample testing support.

* Every batch of products will be tested to ensureits quality.

*The packing also can be according the customers` requirment.

*Any inquiries will be replied within 24 hours.

*we provide Commerical Invoice, Packing List, Bill of loading, COA , Health certificate and Origin certificate. If your markets have any special requirements, let us know.


After-Sales Service

*The fact of logistics information monitoring.

* Any questions about the product can be consulted at any time.

*Product has any problem can return.


Do you accept sample order?

We will make samples before mass production, and after sample approved, we’ll begin mass production. Doing 100% inspection during production, then do random inspection before packing.



You can get free samples for some products,you only need to pay the shipping cost or arrange a courier to us and take the samples. You can send us your product specifications and requests,we will manufacture the products according to your requests.

What’s your MOQ?

Our MOQ is 1kg. But usually we accept less quantity such as 100g on the condition that sample charge is 100% paid.

Do you supply product report?

Yes. We’ll give you product analysis report before shipping.

  Is there a discount?

Different quantity has different discount.


1. ≤50kg, Express delivery recommended, usually called as DDU service;

2. ≤500kg, Air shipping recommended, usually called as FOB, CFR, or CIF service;

3. >500kg, sea shipping recommended, usually called as FOB, CFR, or CIF service;

4. For high value products, please select air shipping and express delivery for safe.

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