Fang Ningtao and others tried to use Salt Lauroylsarcosinate as a decellularization reagent to prepare decellularized tissue engineering scaffold materials, and analyzed their biological properties. Methods: The porcine valved pipeline was decellularized with 0.25% sodium lauryl sarcosine solution and nuclease, and its DNA and soluble protein content were determined, HE, Movat staining, electron microscope observation and biomechanical test ; Rat subcutaneous embedding test to analyze biocompatibility. Results After decellularization, the DNA content in the leaflets and wall of the valved duct was only 1.35% and 2.81% of the normal level, the soluble protein content was only 8.46% and 12.65% of the normal level, and the cellular components were almost completely removed. HE and Movat staining and electron microscope observation showed that the tissue structure was complete, the cell structure completely disappeared, and the main matrix components such as collagen, elastic fiber, and proteoglycan were fully preserved; the biomechanical properties of the valve were not significantly different from those of the normal valve; the biocompatibility of the scaffold Good, with low immunogenicity and degradability. Conclusion: Salt Lauroylsarcosinate is an ideal new decellularization reagent, by which a set of decellularization methods with low toxicity, high efficiency and no damage to tissue structure can be established, and decellularized scaffold materials with excellent biological properties can be prepared.
CN201710937850.8 discloses a descaling agent for drainage pipes. The present invention consists of the following raw materials in parts by weight: 8-16 parts of Salt Lauroylsarcosinate, 5-11 parts of sodium hydroxide, 7-15 parts of polyacrylamide, 4-10 parts of polymaleic anhydride, and 3-7 parts of hydroxyethylene acid , gallic acid 3-9 parts, hydroquinone 7-11 parts, hypochlorite 2-5 parts, citric acid 2-4 parts. The raw material cost of the invention is low, the preparation method is simple, the dirt formed in the drainage pipeline can be effectively removed, the dirt removal ability is strong, no secondary damage is caused to the pipeline, and the bacterial growth of the drainage pipeline is reduced.
CN201210162646.0 discloses a method for extracting Gram-positive bacterial nucleic acid. The invention provides a reagent for breaking the cell wall of Gram-positive bacteria, which consists of extract A and extract B; consists of extract A and extract B; the extract A is prepared according to the following method: Tris, EDTA , SDS, Tween-80, Triton-X-100, Brij-58, 3-[(3-cholesterylaminopropyl)dimethylamino]-1-propanesulfonic acid, Salt Lauroylsarcosinate, β-mercaptoethanol and water mixed , to obtain extract A; the extract B is prepared according to the following method: water-saturated phenol, ethanol and pyrrolidone are mixed to obtain extract B. Experiments of the present invention have proved that the present invention provides a method for efficiently extracting Gram-positive bacterial nucleic acids, which is simple to operate, very low in cost, and has good extraction effects, especially in extracting RNA, which is better than that currently widely used commercially available kits.
