Fang Ningtao and others tried to use Salt Lauroylsarcosinate as a decellularization reagent to prepare acellular tissue engineering scaffolds and analyze their biological properties. Methods: The porcine valved conduits were decellularized with 0.25% Salt Lauroylsarcosinate and nuclease, and the contents of DNA and soluble protein were determined, HE and Movat staining, electron microscope observation and biomechanical test were carried out. ; Biocompatibility analysis of rat subcutaneous embedding assay. Results After decellularization, the DNA content in the valve leaflets and the wall of the valve conduit was only 1.35% and 2.81% of the normal, the soluble protein content was only 8.46% and 12.65% of the normal, and the cellular components were almost completely removed. HE and Movat staining and electron microscope observation showed that the tissue structure was complete, the cell structure completely disappeared, and the main matrix components such as collagen, elastic fibers and proteoglycans were fully retained; the biomechanical properties of the valve were not statistically different from those of the normal valve; the scaffold biocompatibility good, with low immunogenicity and degradability. Conclusion: Salt Lauroylsarcosinate is an ideal new decellularization reagent, which can establish a low-toxicity, high-efficiency decellularization method without damage to tissue structure, and prepare decellularized products with excellent biological properties. Cell scaffold material.
CN201710937850.8 discloses a descaling agent for drainage pipes. The present invention is composed of the following raw materials in parts by weight: 8-16 parts of Salt Lauroylsarcosinate, 5-11 parts of sodium hydroxide, 7-15 parts of polyacrylamide, 4-10 parts of polymaleic anhydride, hydroxy 3-7 parts of ethyl acid, 3-9 parts of gallic acid, 7-11 parts of hydroquinone, 2-5 parts of hypochlorite, 2-4 parts of citric acid. The raw material cost of the invention is low, the preparation method is simple, the dirt formed in the drainage pipeline can be effectively removed, the scale removal ability is strong, the secondary damage to the pipeline is not caused, and the bacterial growth in the drainage pipeline is reduced.
CN201210162646.0 discloses a gram-positive bacterial nucleic acid extraction method. The invention provides a reagent for breaking the cell wall of Gram-positive bacteria, which is composed of extracting solution A and extracting solution B; it is composed of extracting solution A and extracting solution B; the extracting solution A is prepared according to the following method: Tris, EDTA , SDS, Tween-80, Triton-X-100, Brij-58, 3-[(3-cholesterylaminopropyl)dimethylamino]-1-propanesulfonic acid, sodium lauryl sarcosinate, β -Mercaptoethanol and water are mixed to obtain extract A; the extract B is prepared according to the following method: water-saturated phenol, ethanol and pyrrolidone are mixed to obtain extract B. Experiments of the present invention prove that the present invention provides a method for efficiently extracting gram-positive bacterial nucleic acid, which is simple to operate, very low in cost, and has good extraction effect, especially in extracting RNA, which is better than currently widely used commercially available kits.
