Shikonin CAS 517-89-5

Molecular Formula: C16H16O5

Formula Weight: 288.3

ZSpharmac: Shikonin Supplier

Product Name: Shikonin

CAS No: 517-89-5

Purity: 99%

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Basic Info

Product Name:	Shikonin
Other Names:	Shikonin Sigma
CAS:	517-89-5
Place of Origin:	Shandong, China
Brand Name:	ZSpharmac
Type:	Cosmetic Raw Materials
Appearance:	Purple Crystalline Powder
EINECS No.:	NULL
Storage:	-20°C
Provide:	Shikonin MSDS； Shikonin COA

What is Shikonin?

Comfrey is the dry origin of Arnebiaeuchroma (Royle) Johnst. or Arnebiaguttata Bunge., a seasonal herb of the household Boraginaceae. It mostly has naphthoquinones and various other components, and also has chemical components such as shikonin, shikonin, shikonane, shikonin, as well as shikonin polysaccharides. It has a lengthy background of medicinal use in my nation. It is mostly utilized in the therapy of damp macule, purpura, hematuria, stranguria and bloody dysentery, warmth knot bowel irregularity, burns, eczema, erysipelas and more. Modern medical research study has found that comfrey has complicated chemical components and also has a wide variety of medicinal impacts such as anti-tumor, anti-bacterial, anti-virus, anti-HIV, anti-fertility, antipyretic, hemostatic, and hypoglycemic.
Today, the comfrey made use of as medical products is primarily: “soft comfrey”, “difficult comfrey”, as well as “Inner Mongolia comfrey”.
1. Soft comfrey: Uneven long round form, commonly twisted, 7-20cm long, 1-2.5 centimeters in size. The surface is purple-red or purple-brown, and also the skin is loose, in the form of strips and flakes, commonly with more than 10 overlapping layers, which are easy to peel. Residues of stems with noticeable branches at the top. Light-weight, soft and also very easy to damage. The section is uneven, the xylem is tiny, yellow-white or yellow. Gas certain, taste slightly bitter, astringent.
2. Hard comfrey: cone-shaped, twisted, branched, 7-14cm long, 1-2cm in size. The surface area is purplish red or purplish black, harsh with longitudinal lines, and also the skin is slim and also very easy to remove. Hard as well as fragile, very easy to damage. The skin part of the section is dark purple, the wood part is larger, grayish yellow. Minor scent, a little bitter taste.
3. Inner Mongolia comfrey: conical or round, twisted, 6-20cm long, 0.5-4cm in diameter. The origin head is a little thick, with 1 or even more deposits at the top, covered with short bristles. The surface area is purplish red or dark purple, the cortex is slightly thin, as well as the continuous layers are piled, very easy to peel. Difficult and weak, simple to damage. The section is reasonably neat, the skin part is purple red, the wood part is smaller sized, yellowish white. Details gas, astringent preference.

Shikonin Property

Melting point	148℃
Boiling point	567.4±50.0 °C(Predicted)
density	1.373±0.06 g/cm3(Predicted)
storage temp.	-20°C
pka	7.34±0.20(Predicted)
form	Red crystalline solid.

Pharmacological effects of Shikonin

(1) Hypoglycemic result
Comfrey fallen leave remove and comfrey glycan (A, B, C) have obvious hypoglycemic result
( 2) Antibacterial effect.
Comfrey has inhibitory result on Jingke 68-1 virus in examination tube, as well as has repressive impact on Staphylococcus aureus. The anti-parainfluenza infection effect of levoshikonin was studied by hemagglutination response and cytopathic technique, and also the outcomes revealed that it had reduced poisoning within the focus variety made use of in the experiment, as well as had certain in vitro anti-influenza virus activity as well as the capacity to straight eliminate parainfluenza infection. result.
( 3) Effect on blood coagulation
Intraperitoneal shot of shikonin components (shikonin, acetylshikonin) does not impact blood clotting time, however can stop the anticoagulant effect of heparin.
( 4) Anti-tumor impact
Comfrey essence has a particular repressive result on the late phase of DNA synthesis (G2 phase) in Hela cells.
( 5) Anticancer impact
The impact of shikonin on inhibiting proliferation, advertising apoptosis as well as causing cell cycle apprehension of human choriocarcinoma drug-resistant cell line (JAR/MTX) cultured in vitro. Long term, the development inhibition price of choriocarcinoma drug-resistant cells likewise enhanced dramatically.
( 6) Results on hormonal agent secretion
Experiments on the effect of shikonin on the hypothalamus-pituitary-gonadal axis function of teenage female rats showed that the serum hormone level of the shikonin group was significantly lower than that of the negative control group, as well as there was no considerable difference compared with the positive control team. It reveals that shikonin can prevent the feature of hypothalamus-pituitary-gonadal axis in rats.
( 7) Antioxidant effect
Some scientists identified the scavenging capability of shikonin on superoxide radical (O2-) and 1,1-diphenyl-2-picrylhydrazine radical (DPPH), and also its effect on β-carotene/ linolein Inhibition of acid autoxidation systems. The outcomes showed that shikonin had strong scavenging capability on DPPH as well as O2-, as well as had noticeable repressive effect on β-carotene/ linoleic acid autoxidation system. Solid antioxidant capacity.

Production of Shikonin

1. Induction of callus from comfrey

In 1974, scientists prospered in inducing callus from Sclera sclerophylla. This job began making use of plant cell design to produce shikonin. The research study work with medicinal comfrey in my nation only started in the mid-1980s. Follower Hongxia as well as Zhu Ruxing specifically caused the callus of Comfrey yunnanensis and located that its shikonin material was greater than that of its origins. Li Guofeng et al. generated the callus of Xinjiang Zicao with the highest possible material of energetic ingredients.

2. Suspension and also immobilization society of comfrey cells

( 1) Suspension society

Fujita et al. first recommended a two-step technique for suspension society of comfrey cells. Ning Wen et al. performed speculative study and also showed that the cell development curve was level and also S-shaped. After the cells quit growing, shikonin and also its derivatives were formed in huge quantities, and also the dynamic modifications of the two were negatively correlated.

( 2) Synchronous extraction

Utilizing some organic solvents to draw out shikonin synchronously throughout the cultivation procedure not only streamlines the splitting up and also filtration of additional metabolites, however additionally decreases the feedback inhibition of the products.

( 3) Activator large-scale society of comfrey cells

The Institute of Chemistry and Metallurgy of the Chinese Academy of Sciences has efficiently created a new sort of plant cell bioreactor of “air-lift inner flow semi-cross-flow kind”, and also recognized the reactor cultivation of 100 litres of Xinjiang Zikaia cells. Large-scale growing in reactors is an essential action towards industrial manufacturing of comfrey.

( 4) Incapacitated culture innovation

Currently, immobile society has actually become an essential direction in the research study on the manufacturing of additional substances by plant cell culture. Lv Hua et al. incapacitated and also cultured comfrey cells embedded in calcium alginate. After thirty day, the pigment exudation reached 70, while the suspension cells just had 30. If n-hexadecane is utilized for removal at the same time, the pigment secretion can reach 95%. Research studies have actually shown that in the process of debilitated culture, if the tool is changed every 45 days, the cells still have the capacity to generate pigment after 180 days.

( 5) Quick proliferation of comfrey seed startings

Utilizing bioengineering ways to directly generate aseptic vaccines and then plant them in the manufacturing areas, this method has low cost and quick results, which is one of the most direct embodiment of the modernization of traditional Chinese medication. The above two methods are commonly accomplished all at once and also complement each other.

Content Determination of Shikonin

(1) Chromatographic problems Silica gel high-efficiency thin-layer plate, the empty plate is first pressed to the front with ethanol-glacial acetic acid (5:0.2) for pretreatment; n-hexane-acetone-chloroform-glacial acetic acid (8:1:0.1:0.1) is Developing representative; placement under natural light.
( 2) Preparation of recommendation material option Precisely evaluate shikonin, deoxyshikonin, acetylshikonin, β, β-dimethylacryloylarkanin in suitable quantities, and also include chloroform to make each ml consisting of 0.1 mg service, as the reference remedy.
( 3) Prep work of example solution Precisely weigh 0.2 g of comfrey root powder, put it in a 50 ml Erlenmeyer flask with a stopper, include 10.0 ml of chloroform, remove by ultrasonic oscillation for 10 min, centrifuge, and the supernatant is made use of as the sample solution.
( 4) Action 1-3 μl of the sample option and the referral substance solution, respectively, and place on the same high-efficiency thin-layer plate (pretreatment), create according to the above chromatographic problems, take it out, dry it, and also scan it according to the thin-layer scanning method, λS =280nm, λR= 400nm, reflective linear scanning; slit 5mm × 0.4 mm; scanning speed as well as paper speed are both 20mm/min.

( 5) Measurement results

About That Product:
If you need Shikonin, a cosmetic raw material, please contact our service staff directly, and we will provide you with high-quality products at the best price.
As an experienced Shikonin manufacturer and supplier, ZSpharmac will provide Shikonin raw materials to customers all over the world for a long time.
Over the years, the company has been adhering to the spirit of “integrity management, strict quality control, customer first”, and has won unanimous praise from domestic and foreign customers.

Company Profile and Corporate Culture

Company Profile：

ZhiShang Chemical is owned by ZhiShang Group is a professional new-type chemicals enterprise combined into research and development, production and sales .

The company’s competitive product is pharmaceutical raw materials and intermediates (especially carbohydrate derivatives Series), In recent years, the company has made a major breakthrough in food and feed additives, plant extraction, industrial chemicals industry .

The company insists on the spirit of “sincere management, strict quality control, customer as god” , get consistent high praise from customers at home and abroad.

Corporate Culture：

OUR MISSION
Help China Chemicals to benefit the happiness of human life
OUR VISSION
Become the most trusted chemical supplier in the world
OUR BUSINESS PHILOSOPHY
Striver – oriented, enrich employees, customer first, deep service, seek development
OUR VALUES
Be prepared for danger in times of peace, forge ahead actively, unity and cooperation, and be brave to fight

About Us

The production base is located in Zhangqiu chemical industry park and Tai’an high-tech chemical industry park. laboratory and workshop in strict accordance with the GMP standard and the product fit national ISO9001 and ISO2000 standards.

“Zhishang” products are exported to Europe, North and South America, the Middle East, Asia Pacific and Africa area, so as to establish a long-term and stable cooperation relationship with customer in the world.

Company Info

Business Type: Manufacturer
Product Range: Cosmetic raw materials
Products/Service:Cosmetic raw materials (flavors and fragrances,antioxidant products, moisturizing and hydrating, whitening agents, cosmetic additives)
Total Employees: 51~100
Capital (Million US $): 10000000RMB
Year Established: 2016

Production Capacity

No. of Production Lines : 8
No. of QC Staff : 5 -10 People
OEM Services Provided : yes
Factory Size (Sq.meters) : 3,000-5,000 square meters
Certificate: ISO9001 , CE , GMP , API , MSDS
Factory Location : Diao Town Industry Park, Zhangqiu City, Jinan City, Shandong Province, China.

Service

Pre-Sales Service

* Prompt reply and 24 hours online, professional team to provide best price and high quality product.

* Sample testing support.

* Every batch of products will be tested to ensureits quality.

*The packing also can be according the customers` requirment.

*Any inquiries will be replied within 24 hours.

*we provide Commerical Invoice, Packing List, Bill of loading, COA , Health certificate and Origin certificate. If your markets have any special requirements, let us know.

After-Sales Service

*The fact of logistics information monitoring.

* Any questions about the product can be consulted at any time.

*Product has any problem can return.

FAQ

Do you accept sample order?

We will make samples before mass production, and after sample approved, we’ll begin mass production. Doing 100% inspection during production, then do random inspection before packing.

HOW TO CONFIRM THE PRODUCT QUALITY BEFORE PLACING ORDERS?

You can get free samples for some products,you only need to pay the shipping cost or arrange a courier to us and take the samples. You can send us your product specifications and requests,we will manufacture the products according to your requests.

What’s your MOQ?

Our MOQ is 1kg. But usually we accept less quantity such as 100g on the condition that sample charge is 100% paid.

Do you supply product report?

Yes. We’ll give you product analysis report before shipping.

Is there a discount?

Different quantity has different discount.

Shipping

1. ≤50kg, Express delivery recommended, usually called as DDU service;

2. ≤500kg, Air shipping recommended, usually called as FOB, CFR, or CIF service;

3. >500kg, sea shipping recommended, usually called as FOB, CFR, or CIF service;

4. For high value products, please select air shipping and express delivery for safe.