1500kg star anise dried fruit (the shikimic acid content is 3.69%) is pulverized to a particle size of over 20～30 meshes and placed in a percolation tank. According to the mass ratio of material: water=1:3, water is passed into the material and heated under normal pressure. The water temperature reaches 75°C to soak the material for 1 hour, turn on the motor for 15 minutes, and then soak for 1 hour. Repeat this for 5 times. After soaking and extracting for 7 hours, after concentrating the leachate in the inner circulation concentration tank, the specific gravity is 1.15, and the weight is 1.15. It is 45.7kg of crude shikimic acid extract.
2 Mix the crude extract of Shikimic Acid according to the ratio of material: silica gel = 1:1 while it is still hot, stir evenly, cool to dryness, and use it to load the sample and pass it through the chromatographic column.
3. Put the cooled, dried and stirred silica gel samples on the upper ends of 6 chromatographic columns with an inner diameter of 40 cm, and the sample thickness is about 15 cm. The upper Shikimic Acid Sigma sample was rinsed at a flow rate of about 800ml/min.
4. Detect the eluent that flows out with a high-performance liquid phase detector, and start collecting when it is detected that the eluent contains shikimic acid. After 5 hours of collecting, it is specially noted that the ethyl acetate pipeline is realized in this process, Loop operation. The detection conditions of Shikimic Acid Sigma are mobile phase: double distilled water adjusted to pH 2 with phosphoric acid; flow rate: 0.6ml/min; wavelength: 213nm; column temperature 25℃; column C18; Concentration 20 μg/ml.
5. Concentrate the collected liquid to dryness, add 15L of methanol to dissolve, then add 45L of water, stand for refrigerated crystallisation, filter the crystals the next day to obtain 18kg of dry pure shikimic acid, and the detected content is 92%.
6. Recrystallize once by the same crystallization method in step 5 to obtain 16kg of fine shikimic acid with a content of 98%, and the final extraction rate of Shikimic Acid is 85%.