A method for measuring sesamolin content in sesame, comprising the following steps:
(1) To make a standard working curve: use methanol solutions of sesamolin standard substances with gradient concentrations of 200, 100, 50, 25, 12.5, and 6.25 μg/mL, and use anhydrous methanol as a blank control to measure the absorbance at a wavelength of 287 nm , with the concentration as the abscissa and the absorbance as the ordinate, draw a standard working curve, and the corresponding fitting equation is y=457.729323x-3.3962015, where y is the peak area, and x is the sesamolin content;
(2) Sample treatment: crush the sesame sample, pass it through a 0.35 μm sieve, weigh 0.2000 g of the obtained powder, add 7 mL of absolute ethanol, and use ultrasonic waves (ultrasonic power: 80 W) and oscillator for 25 min intermittently at 60 ° C (Sonication for 5 minutes, shaking for 1 minute, and so on cycle), then, centrifuge at 5000rpm for 2min, take the supernatant in a syringe, and filter it with a 0.45μm nylon membrane filter head; this step uses a digitally controlled ultrasonic cleaner for ultrasonic treatment. Maximum frequency: 40KHZ, maximum power: 150W;
(3) HPLC determination, determination conditions: chromatographic column: Agilent L1260, Eclipse XDB-C18-USP L1 analytical column, specification: 4.6 mm×250 mm, particle size: 5 μm, original product number: 990967-202, column temperature: 30 ℃; mobile phase is methanol: water = 80:20 (V/V), flow rate: 1mL/min, detection wavelength: 287nm, injection volume: 9μL, retention time: 15min; search or calculate according to the prepared standard working curve, That is, the content of sesamolin in the sesame sample was obtained.