Salvianolic Acid B CAS 115939-25-8

Molecular Formula: C36H30O16

Formula Weight: 718.61

ZSpharmac: Salvianolic Acid B Supplement

Product Name: Salvianolic Acid B

CAS No: 115939-25-8

Purity: 99%

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Basic Info

Product Name:	Salvianolic Acid B
Other Names:	Lithoric Acid B
CAS:	115939-25-8
Place of Origin:	Shandong, China
Brand Name:	ZSpharmac
Type:	Cosmetic Raw Materials
Appearance:	Pale Yellow to Yellow Solid
EINECS No.:	0
Storage:	-20°C Freezer
Provide:	Salvianolic Acid B MSDS； Salvianolic Acid B COA

What is Salvianolic Acid B?

Cardiovascular disease is an illness that seriously threatens human wellness. It has the characteristics of “high morbidity, high disability rate, as well as high death price”. It has actually become the primary reason for human death. Consequently, locating drugs that can successfully deal with cardiovascular disease has actually become the key professional trouble. The traditional Chinese medication for advertising blood flow as well as getting rid of blood tension, Salvia miltiorrhiza, has actually been commonly made use of in the treatment of heart diseases as a result of its functions of getting rid of blood tension and also eliminating discomfort, promoting blood flow and also removing the meridians. Researches have found that salvianolic acids are the most bountiful water-soluble compounds in Salvia miltiorrhizabunge (Latin name: salviamiltiorrhizabunge), while salvianolic acid B is the most bountiful and biologically energetic substance in salvianolic acid. Studies have actually shown that salvianolic acid not only has a safety effect on myocardial ischemia/reperfusion injury, yet additionally has a safety effect on heart attack as well as myocardial cell hypertrophy.

Salvianolic Acid B Properties:

Melting point	>149oC (dec.)
storage temp.	-20°C Freezer
solubility	DMSO (Slightly), Ethanol (Slightly), Methanol (Slightly)
form	Solid
color	Pale Yellow to Yellow

Pharmacological Action and Application of Salvianolic Acid B

1. The role of salvianolic acid B in cardiovascular disease

1) The protective effect of on myocardial infarction: The myocardial infarction (MI) model in rats was constructed by coronary ligation, and the protective effect of salvianolic acid B on MI was investigated. The results showed that compared with the control group, salvianolic acid B could exert a significant cardioprotective effect on MI. Mechanistic studies have shown that salvianolic acid B mainly inhibits the up-regulation of leptin and endothelin-reactive oxygen species (ET-ROS) and restores myocardial sarcoplasmic reticulum calcium ATPase 2a (sarco/endoplasmicreticulum ATPase 2a, SERCA2a) and phosphorus receptors in the myocardium. The normal expression of the protein, and then play a role in protecting the heart.

2) The protective effect of on MI/R injury: a large number of free radicals will be generated during MI/R, which will enhance the lipid peroxidation of cell membranes, change membrane fluidity and permeability, and lead to abnormal electrophysiological activities. , induce and promote arrhythmia. Due to the enhanced lipid peroxidation in myocardial cells, the content of peroxidation products such as malondialdehyde (MDA) in the myocardial ischemia area increases; lactate dehydrogenase (LDH) and creatine phosphokinase in coronary effluent (creatinephosphokinase, CPK) increased and myocardial tissue superoxide dismutase (superoxidedismutase, SOD) decreased. The study found that salvianolic acid B can significantly increase the SOD activity in myocardial tissue of MI/R-injured rats, and can effectively resist the toxic effect caused by oxygen free radicals, thereby reducing myocardial cell damage. The protective effect of salvianolic acid B on MI/R injury was investigated by constructing a rat MI/R model, and it was found that compared with the control group, administration of salvianolic acid B could significantly increase the levels of troponins (CTn) and creatine in plasma. The content of kinase isoenzyme (creatinekinase-MB, CK-MB), while significantly reducing the levels of nitric oxide (nitricoxide, NO) and total superoxide dismutase (total superoxide dismutase, TSOD) in plasma, indicating that Rat MI/R injury has obvious protective effect. Further research found that salvianolic acid B can alleviate oxidative stress, reduce calcium overload and improve endothelial function.

3) Has the effect of inhibiting cardiomyocyte hypertrophy: The effect of salvianolic acid B on angiotensinⅡ (AngⅡ)-induced cardiomyocyte hypertrophy in neonatal rats was investigated. The results showed that salvianolic acid B could inhibit AngⅡ Induced cardiomyocyte hypertrophy, decreased atrial natriuretic peptide and brain natriuretic peptide mRNA expression and decreased cell surface area. Mechanistic studies found that salvianolic acid B could inhibit the activity of poly(ADP-ribose)polymerase-1 (PARP-1); at the same time, it was found that overexpression of PARP-1 could attenuate the activity of salvianolic acid B Anti-cardiomyocyte hypertrophy effect, these studies show that salvianolic acid B has a good inhibitory effect on cardiomyocyte hypertrophy.

4)Can inhibit the formation of atherosclerosis: The effect of salvianolic acid B on atherosclerosis was investigated through experiments, and the results showed that the treatment of vascular smooth muscle cells with salvianolic acid B can effectively inhibit platelet-derived growth factor ( platelet-derived growth factor, PDGF)-induced cell proliferation and migration, and can significantly increase the expression of heme oxygenase-1 (hemeoxygenase-1, HO-1); at the same time, salvianolic acid can significantly reduce reactive oxygen species (reactive oxygen species) , ROS) production and reduction of nicotinamide adenine dinuclear

The ratio of nucleotide phosphate/reduced nicotinamide adenine dinucleotide phosphate (NADP/NADPH). Using human umbilical vein endothelial cells, it was found that salvianolic acid B can significantly induce the production of HO-1, and at the same time effectively inhibit the activation of nuclearfactor-κB (NF-κB) induced by tumor necrosis factor-α (TNF-α). Inhibition of HO-1 expression by siRNA abolished the protective effect of salvianolic acid on vascular endothelial cells and blocked the proliferation, migration and inflammation of HO-1-deficient cells. Further mechanism studies have shown that salvianolic acid B induces the expression of HO-1 by activating nuclear factor-E2-related factor2 (Nrf2), thereby inhibiting the proliferation, migration and inflammation of vascular cells, thereby exerting anti-inflammatory effects. The role of atherosclerosis.

5) Can effectively reduce portal hypertension: Endothelin-1 (ET-1) was used to induce portal hypertension in mice, and the effect of salvianolic acid B on blood microcirculation in the liver of mice with portal hypertension was investigated. , the results showed thatcompared with the control group,could significantly reduce the average blood flow and microcirculation flow rate in the liver of portal hypertension mice, and then play a role in reducing portal hypertension. Studies have shown that salvianolic acid B can reduce portal hypertension in rats, and studies have found that salvianolic acid B mainly acts through macrophages in the rat liver.

2. The mechanism of salvianolic acid B regulating cardiovascular effects

1) Akt-eNOS signaling mediates the protective effect of salvianolic acid B on MI/R injury: studies have found that Akt-eNOS is one of the components of the reperfusion injury salvagekinase (RISK) signal transduction pathway. The expression of related proteins has a protective effect on the heart. In this pathway, Akt is the central link and the main target enzyme. Only phosphorylated Akt can exert a series of biological activities. The rat MI/R injury model was prepared by ligating the coronary artery, and the protective effect of salvianolic acid B on MI/R injured rats was investigated. Studies have shown that can increase the phosphorylation level of Akt and lead to its activation. Akt further activates its downstream target eNOS to phosphorylate it, and then exerts a protective effect on MI/R injury through a series of downstream biological effects.

2) TLR4-NFκB-TNF-α inflammatory pathway mediates salvianolic acid B to protect cardiomyocyte injury: lipopolysaccharide (LPS) induces inflammatory injury of neonatal rat cardiomyocytes, and explores the effect of salvianolic acid B on cardiomyocyte injury protective effect. The results showed that the concentrations of lactate dehydrogenase and tumor necrosis factor-α in this group were significantly higher than those in other groups after LPS treatment, indicating that the modeling was successful; while after treatment with salvianolic acid B, the concentrations of LDH and TNF-α decreased significantly. . At the same time, immunohistochemical results showed that the expressions of Toll-like receptor 4 (TLR4) and NF-κB were significantly increased after LPS administration; expression decreased significantly. This indicates that in the early stage of cardiomyocyte injury induced by LPS, the TLR4-NF-κB-TNF-α pathway was rapidly activated, and played a role in protecting cardiomyocyte injury, probably by inhibiting this pathway. realized.

3) Autophagy-mediated salvianolic acid B to protect cardiomyocyte injury: The effect of autophagy on starved cardiomyocytes was investigated experimentally, and the effect of salvianolic acid B on autophagy in starved cardiomyocytes was also observed. The results showed that autophagy was induced in cardiomyocytes after 3 h of starvation, which led to cell damage, and the expression of LC3-II in starved cells was found to increase in a time-dependent manner. After administration of salvianolic acid B, LC3-II was significantly inhibited. At the same time, the cell viability and the level of ATP in cardiomyocytes also increased significantly. This study shows that salvianolic acid B can play a protective role by blocking excessive autophagy.

4) Mitochondrial apoptosis pathway mediates salvianolic acid B to inhibit myocardial cell apoptosis after myocardial infarction: The myocardial infarction model in rats was constructed by ligating the left anterior descending coronary artery, and then the effect of on myocardial cell apoptosis in myocardial infarction rats was investigated. impact of death. The results showed that salvianolic acid B could significantly reduce the apoptosis rate of cardiomyocytes. The authors further investigated the effect of salvianolic acid B on the expression of apoptosis-related genes, and found that could significantly reduce the expression of Bax, and also found that also significantly reduced the expression of STAT-3 and IL-6. This indicated that the mitochondrial apoptosis pathway was involved in regulating the apoptosis of cardiomyocytes after myocardial infarction by salvianolic acid B.

Study on Pharmacological Effects of Salvianolic Acid B

Tang Zhongjie et al. verified the effect of the SOX2OT/miRNA (miR)-34a/SOX2 signaling axis on the invasion and metastasis of human colorectal cancer LOVO cells and that salvianolic acid B (SalB) inhibited the invasion and metastasis of LOVO cells in a concentration-dependent manner through this signaling axis. METHODS: Dual-luciferase reporter gene was used to detect the targeted binding of SOX2OT to miR-34a, miR-34a and SOX2; SOX2OT plasmid and miR-34a mimic were transiently transfected into LOVO cells, and the transfection efficiency was verified by RT-qPCR; RT-qPCR , Western blot to detect the correlation of the expressions of SOX2OT, miR-34a and SOX2; scratch test and Transwell test to detect the effect of SOX2OT/miR-34a/SOX2 axis on the invasion and migration of LOVO cells; MTT method to detect the effect of salvianolic acid B on LOVO Growth inhibition; RT-qPCR detection of the expression of SOX2OT under the treatment of low, medium and high concentrations of salvianolic acid B; scratch assay and Transwell assay to detect the invasion and migration of cells under the treatment of low, medium and high doses of salvianolic acid B. Results: There was targeted binding between SOX2OT and miR-34a, miR-34a and SOX2 in LOVO cells; SOX2OT inhibited miR-34a and up-regulated SOX2; miR-34a could down-regulate the expressions of SOX2OT and SOX2; miR-34a reversed the effect of SOX2OT on cell invasion In a certain concentration range, inhibited the growth of LOVO cells in a dose-effect relationship; salvianolic acid B inhibited the expression of SOX2OT in LOVO cells in a concentration-dependent manner; inhibited the invasion and metastasis of LOVO cells, and the As the concentration increased, the inhibitory effect was enhanced. Conclusion: The SOX2OT/miR-34a/SOX2 signaling axis is closely related to the invasion and metastasis of LOVO cells, and salvianolic acid B inhibits the invasion and metastasis of LOVO cells by inhibiting this signaling axis in a dose-effect relationship.

Guo Piaoting et al. verified that salvianolic acid B (SalB) inhibits the proliferation and promotes apoptosis of human colorectal cancer cell HCT-116, and further elaborated its possible mechanism through reactive oxygen species (ROS). Methods: Colorectal cancer cells HCF-116 were cultured in vitro and divided into HCT-116 group, HCT-116+H2O2 group, HCT-116+SalB group, HCT-116+SalB+N-acetyl-L-cysteine (N- acetylcysteine, NAC) group. After group intervention, MTT assay was used to detect cell viability, plate cloning assay to detect cell proliferation, flow cytometry to detect ROS content, cell cycle and cell apoptosis rate. Results: SalB had inhibitory effect on HCT-116 cells, and was positively correlated within a certain concentration range (P<0.01); SalB and H2O2 promoted the generation of ROS in HCT-116 cells (P<0.01), and pretreatment with ROS scavenger NAC Cleared the ROS generated by SalB (P<0.01); SalB inhibited the proliferation of HCT-116 cells (P<0.01) and promoted its apoptosis (P<0.01), which could be partially reversed by NAC (P<0.05); SalB induced G0/G1 cycle arrest of HCT-116 cells (P<0.01), NAC pretreatment completely reversed the cycle arrest caused by SalB (P<0.05). Conclusion: SalB can induce cell cycle arrest by increasing the level of ROS in HCT-116 cells, thereby inhibiting cell proliferation and promoting apoptosis.

Preparation of Salvianolic Acid B

Method 1: a preparation method of high-purity salvianolic acid B:

a. First, pulverize the Chinese medicinal material Salvia miltiorrhiza. For the first time, add the crushed salvia miltiorrhiza under the condition of slight boiling of deionized water at 100 degrees for 1 hour, and use water 5 times the weight of Salvia miltiorrhiza; then under the condition of less than 50 degrees PH1-6.5 Extract 2-3 times, each time for 40 minutes, use 3 times the weight of Salvia miltiorrhiza water;

b. The extract is filtered and then chromatographed by macroporous adsorption resin, eluted with water, desorbed with 20—90% C1—C5 lower alcohol, and concentrated under reduced pressure to obtain salvianolic acid B the crude product;

c. The crude product of is further subjected to cellulose chromatography, eluted with lower alcohol or acetone, desorbed with water or lower alcohol of C1—C5, combined with the components containing salvianolic acid B, concentrated under reduced pressure, Freeze-drying to obtain high-purity salvianolic acid B.

d. In steps a, b and c, adjust the pH to 1-6.5 with a pH adjusting substance.

Method 2: a method for preparing high-purity salvianolic acid B by salting out, comprising the following steps:

(1) After crushing Salvia miltiorrhiza, extract it with alcohol solvent, water or alcohol-water mixture, filter and concentrate under reduced pressure to obtain a paste-like crude salvia miltiorrhiza extract;

(2) Simple purification treatment of the crude Salvia miltiorrhiza crude extract to obtain a crude crude extract; the purification treatment adopts precipitation, extraction, macroporous resin chromatography, forward chromatography or reverse chromatography; precipitation The method includes acid precipitation, water precipitation and alcohol precipitation. Water precipitation is to precipitate out water-insoluble low-polarity impurities, alcohol precipitation is to precipitate out alcohol-insoluble impurities, and acid precipitation is to precipitate out insoluble impurities under acidic conditions. Macroporous resins include weak polar and non-polar resins such as D101, AB-8, HPD-100, etc. Reversed-phase chromatography packings include reversed-phase silica gel and reversed-phase resins such as C18 silica gel, CG161, Sephadex LH-20, and the like.

(3) Dissolve the crude Salvia miltiorrhiza crude extract or the crude Salvianolic acid B crude extract in an organic solvent to obtain a crude solution of Salvianolic acid B, and add a salt-forming agent dropwise under slow stirring, and the pH of the salt-forming pH is 3-4 , the salt-forming temperature is 25-45 ° C, and the precipitated salvianolic acid B salt is precipitated; The weight percentage content of salvianolic acid B is 5～80%, and the molar weight of the salt-forming agent is 1-2.5 times of the molar weight of salvianolic acid B, and the salt-forming agent is a weak base solution and potassium, sodium, calcium, magnesium, ammonia, etc. , amine organic salt solution or potassium, sodium, calcium, magnesium, ammonia, amine carbonate solution; the organic solvent is selected from methanol, ethanol, propanol, acetone, acetonitrile, tetrahydrofuran, dioxane, dimethyl One or both of sulfoxide, formamide or ethyl acetate, preferably a mixed solution of ethyl acetate and absolute ethanol;

(4) Desalting salvianolic acid B salt by acid precipitation desalting method, extraction desalting method or macroporous resin chromatography desalting method, and drying to obtain high-purity .

Company Profile and Corporate Culture

Company Profile：

ZhiShang Chemical is owned by ZhiShang Group is a professional new-type chemicals enterprise combined into research and development, production and sales .

The company’s competitive product is pharmaceutical raw materials and intermediates (especially carbohydrate derivatives Series), In recent years, the company has made a major breakthrough in food and feed additives, plant extraction, industrial chemicals industry .

The company insists on the spirit of “sincere management, strict quality control, customer as god” , get consistent high praise from customers at home and abroad.

Corporate Culture：

OUR MISSION
Help China Chemicals to benefit the happiness of human life
OUR VISSION
Become the most trusted chemical supplier in the world
OUR BUSINESS PHILOSOPHY
Striver – oriented, enrich employees, customer first, deep service, seek development
OUR VALUES
Be prepared for danger in times of peace, forge ahead actively, unity and cooperation, and be brave to fight

About Us

The production base is located in Zhangqiu chemical industry park and Tai’an high-tech chemical industry park. laboratory and workshop in strict accordance with the GMP standard and the product fit national ISO9001 and ISO2000 standards.

“Zhishang” products are exported to Europe, North and South America, the Middle East, Asia Pacific and Africa area, so as to establish a long-term and stable cooperation relationship with customer in the world.

Company Info

Business Type: Manufacturer
Product Range: Additive , Chemical Auxiliary & Catalyst , Organic Chemicals
Products/Service: Organic Intermediate,Inorganic Chemistry, APIs, Dyestuffs And Pigments, Fragrance And Spices, Food Additives
Total Employees: 51~100
Capital (Million US $): 10000000RMB
Year Established: 2016

Production Capacity

No. of Production Lines : 8
No. of QC Staff : 5 -10 People
OEM Services Provided : yes
Factory Size (Sq.meters) : 3,000-5,000 square meters
Certificate: ISO9001 , CE , GMP , API , MSDS
Factory Location : Diao Town Industry Park, Zhangqiu City, Jinan City, Shandong Province, China.

Service

Pre-Sales Service

* Prompt reply and 24 hours online, professional team to provide best price and high quality product.

* Sample testing support.

* Every batch of products will be tested to ensureits quality.

*The packing also can be according the customers` requirment.

*Any inquiries will be replied within 24 hours.

*we provide Commerical Invoice, Packing List, Bill of loading, COA , Health certificate and Origin certificate. If your markets have any special requirements, let us know.

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*The fact of logistics information monitoring.

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*Product has any problem can return.

FAQ

Do you accept sample order?

We will make samples before mass production, and after sample approved, we’ll begin mass production. Doing 100% inspection during production, then do random inspection before packing.

HOW TO CONFIRM THE PRODUCT QUALITY BEFORE PLACING ORDERS?

You can get free samples for some products,you only need to pay the shipping cost or arrange a courier to us and take the samples. You can send us your product specifications and requests,we will manufacture the products according to your requests.

What’s your MOQ?

Our MOQ is 1kg. But usually we accept less quantity such as 100g on the condition that sample charge is 100% paid.

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Yes. We’ll give you product analysis report before shipping.

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Different quantity has different discount.

Shipping

1. ≤50kg, Express delivery recommended, usually called as DDU service;

2. ≤500kg, Air shipping recommended, usually called as FOB, CFR, or CIF service;

3. >500kg, sea shipping recommended, usually called as FOB, CFR, or CIF service;

4. For high value products, please select air shipping and express delivery for safe.