1) Anti-aging: Using the combination of in vivo and in vitro research methods, the effect of the medicinal plant Rhodiola rosea extract – rhodiola on in vitro cultured human embryonic lung diploid fibroblasts (2BS) and rat liver was discussed. , observed the effect of Rhodiolin on the growth and proliferation of 2BS cells, as well as on the morphological and histochemical changes of rat liver cells and the changes of lipid peroxide (LPO) and liver serum superoxide dismutase (SOD) in liver tissue. The experimental results are that Rhodiolin can promote the growth and proliferation of 2BS cells, and reduce the content of LPO and lipofuscin in liver tissue and the activity of acid phosphatase (ACPase) in rat liver tissue. The results show that Rhodiolin has the effect of delaying cell aging and anti-degeneration.
2) Anti-senile dementia: In experiments, D-galactose was injected intraperitoneally for 6 consecutive weeks, and Aβ140 was injected into the bilateral hippocampus to create AD rat model. The learning and memory behaviors of rats were detected by Y-shaped maze and one-time passive avoidance test. The content of lipofuscin in cerebral cortex, the fluidity of hippocampal mitochondrial membrane, SOD activity and MDA content were measured, and the ultrastructure of hippocampus was observed by electron microscope. Results Rhodiolin could significantly improve the learning and memory impairment of dementia rats, significantly reduce SOD activity, MDA content and cortical lipofuscin content in the hippocampus of dementia rats, and inhibit the degeneration and apoptosis of hippocampal neurons. Therefore, Rhodiolin may have a certain preventive effect on senile dementia rats by affecting the level of free radicals
3) The effect of Rhodiolin on biochemical indicators such as blood sugar in diabetic rats. Of 25 SD rats, 5 were randomly selected as blank control group, and the remaining 20 rats were treated with alloxan-induced diabetes. After successful modeling, they were randomly divided into experimental group (10 rats) and control group (10 rats). The experimental group was treated with Rhodiola angustifolia extract – rhodiola, which was injected into the experimental group by tail vein injection every 3 days, and the blood was taken by decapitation on the 32nd day, and the blood glucose, transaminase, urea nitrogen and blood routine indexes of each rat were measured. . Results Rhodiolin had no significant effect (P>0.05) on blood glucose, BUN, MCV, MCH, MCHC, and PLT in diabetic rats; however, GPT, RBC, HCT, HGB, RDW, and WBC had significant effects compared with the control group. sex (P<0.05) increased. Therefore, Rhodiolin can change the normal physiological function of the liver.
4) Anti-tumor: To explore the effect of Rhodiolin on liver cancer by combining in vitro cultured liver cancer cells (7721) and mouse transplanted liver cancer (H22). The experimental results show that salidroside can inhibit the growth and proliferation of 7721 and H22 hepatoma cells, reduce DNA synthesis, increase glycogen content, improve the quality of life and prolong the survival period of tumor-bearing mice. The results of flow cytometry showed that Rhodiolin could inhibit the S phase (DNA synthesis phase) of hepatoma cells cultured in vitro. It is suggested that salidroside has a certain inhibitory effect on 7721 and H22 hepatoma cells.