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Quest Lipase CAS 9001-62-1

Molecular Formula: C11H9N3NaO2+

Formula Weight: 238.19783

ZSpharmac: Quest Lipase Supplier

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Product Name: Quest Lipase
CAS No: 9001-62-1
Purity: 99%

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Basic Info

Product Name:Quest Lipase
Other Names:Lipase
CAS:9001-62-1
Place of Origin:Shandong, China
Brand Name:ZSpharmac
Type:Cosmetic Raw Materials
Appearance:Yellow-Brown Solution
EINECS No.:232-619-9
Storage:2-8°C
Provide:Quest Lipase MSDS;
Quest Lipase COA

What is Quest Lipase?

Lipase (Lipase, glycerol ester hydrolase) comes from the training course of carboxyl ester hydrolase, which can considerably hydrolyze triglycerides right into glycerol in addition to fats. Lipase exists in fat-containing pet, plant along with microbial (such as mold and mildew and also mold, microbes, and so on) tissues. Consisting of phosphatases, sterolases in addition to carboxylesterases. Fats are thoroughly utilized in food, medicine, all-natural leather, everyday chemical market, and so on. Lipase generally exists in pets, plants as well as additionally microbes.
Mission Lipase is a course of enzymes with countless catalytic capacities, which can catalyze the hydrolysis, alcoholysis, esterification, transesterification as well as additionally turn around synthesis of triacylglycerides as well as additionally various other water-insoluble esters. On top of that, it likewise shows a couple of other enzyme jobs, such as phospholipase, lysophospholipase, cholesterol esterase, acyl peptide hydrolase job, and so forth (Hara; Schmid). The different activities of Pursuit Lipase rely upon the attributes of the action system, such as advertising ester hydrolysis at the oil-water interface, and also chemical synthesis along with transesterification in the all-natural stage.

Quest Lipase Property

density 1.2
vapor pressure 0.004Pa at 25℃
storage temp. 2-8°C
solubility H2O: 2 mg/mL, hazy with insoluble particles, faintly yellow
form solution
color yellow-brown
Water Solubility It is soluble in water.

 

Application of Quest Lipase

Application of Quest Lipase: Lipase has a great contribution in food, medicine and washing. Lipase produces cocoa butter substitute, the raw material for making chocolate, and modifies low-priced palm oil into cocoa butter substitute with high price, which has great practical significance for the food industry. Lipase acts on milk esters and produces fatty acids, which impart a unique flavor to dairy products. Lipase plays a key role in the production of enzyme-modified cheese products. The free fatty acid contained in enzyme-modified cheese is more than 10 times higher than that of ordinary-treated cheese, which is very beneficial for it to be used as a flavor enhancer. Lipase can also improve the baking quality of flour products, improve the texture of bread, and prolong the shelf life of products. In the field of medicine, with the increasing awareness of environmental protection, adding lipase to detergent can greatly improve its decontamination effect, and lipase is a biological product, which is easy to be degraded and does not pollute the environment; in the process of leather processing It is necessary to remove the fat from the fur.

After the second revision of the “Food Safety Law of the People’s Republic of China” in 2021, the importance of scientific and technological investment in the food industry has risen sharply, and the importance of lipase research to the food industry is also self-evident, but Quest Lipase is used in other industrial production And research also plays a very important role, lipase is widely used in food processing and quality improvement. Flavoring dairy products and butter as mentioned above. Utilize the site hydrolysis feature to catalyze the ester bond in the oil, so as to improve the nutritional value of edible oil. In fish processing, lipase is used to decompose the lipids in fish meat, and lipase is used to catalyze the transesterification reaction to convert palm oil into cocoa butter. The addition of lipase during the fermentation of alcoholic beverages can alter the taste of the product. Adding lipase to the dough to partially hydrolyze triglycerides and increasing the content of monoglycerides can delay deterioration, and the form of monoglycerides and diglycerides can improve the properties of protein bubbles.

In medicine Quest Lipase is used as a diagnostic tool for diseases such as acute pancreatitis and pancreatic injury. Malnutrition in the treatment of malignant tumors and gastrointestinal disorders. Lipase is used to produce polyunsaturated fatty acids, and the health care effect played by polyunsaturated fatty acids is more and more used by people.

The function of fat in feed in livestock and poultry is mainly to oxidize and supply energy, and the energy it contains is 2.25 times that of carbohydrates. The digestive function of young poultry and young animals is not yet fully developed, and the secretion of endogenous digestive enzymes is insufficient, resulting in malnutrition and affecting production. Lipase can effectively degrade fat, so that young poultry and young animals can digest better.

In recent years, with the rise of cell engineering, genetic engineering, immobilization technology, and the popularization of biological knowledge to the public, products with natural ingredients added to food, milk, perfume, cosmetics and medicine are becoming more and more popular among consumers. . Compounds biosynthesized from natural substrates are considered natural products, while products produced chemically from the same raw materials are not. Therefore, natural ingredients will have a great demand in the future, which makes biocatalysts very attractive. Therefore, Quest Lipase has bright application prospects in the fields of oil, food, medicine, and cosmetics.

Preparation of Quest Lipase

A method for extracting and purifying lipase by using an immobilized horse antibody, comprising the steps of:
S1: Place a single colony of lipase-producing Pseudomonas ATCC21808 in a 250mL shake flask containing 25mL shake flask culture medium, culture it at 28°C and 200rpm for 20h, and then transfer it to fermentation at a 10% inoculum size In the culture medium, cultured at 28°C and 200rpm for 68 hours to obtain a fermentation broth; wherein, each liter of shake flask culture fluid included: 5g of yeast extract, 5g of peptone, 10g of vegetable oil, and the balance was water; the pH value was 7.0; Each liter of fermentation broth includes: 10g of yeast extract, 5g of peptone, 0.5g of NaCl, 10g of vegetable oil, and the balance is water; the pH value is 7.0;
S2: Centrifuge the fermentation broth, collect the supernatant, and obtain a crude lipase enzyme liquid; wherein, the centrifugation is performed by using a high-speed refrigerated centrifuge at 4°C and 10800g for 15 minutes;
S3: Put the lipase crude enzyme solution on the immobilized horse antibody column. During the loading period, detect the lipase activity of the effluent until the lipase activity flows out, stop the solution; then use 0.1M hydrogen phosphate with a pH value of 7.2 Wash the column with disodium-potassium dihydrogen phosphate buffer, then elute with 0.1M disodium hydrogen phosphate-citric acid buffer with a pH value of 3, collect the eluate, and obtain purified lipase; and after the enzyme flows out , Immediately wash the column with a 1M disodium hydrogen phosphate-potassium dihydrogen phosphate buffer solution with a pH value of 8 to neutrality, and also use this solution to adjust the eluent to a pH value of 7; wherein, the preparation of the immobilized horse antibody column The method includes the steps of: selecting 4 Mongolian horses (the weight and the corresponding age are respectively: the weight is 240 kg and the age is 3 years old, the weight is 290 kg and the age is 5 years old, the weight is 320 kg and the age is 7 years old, the weight is 360 kg and the age is 10 years old), immunization once every two weeks, a total of 12 weeks of immunization, 2 mL each time, the dose is 15 mg lipase plus the same amount of Freund’s complete adjuvant, multi-point subcutaneous immunization, ELISA detection antibody The antiserum was collected after the titer was greater than 1:10000; the antiserum was centrifuged to remove cell components and other precipitates, and the supernatant was collected and the polyclonal antibody was purified by proteinA or proteinG affinity chromatography column; the polyclonal antibody was mixed with activated Sepharose2B was connected to obtain an immobilized horse antibody column;
S4: Add polyethylene glycol to the purified lipase to precipitate the lipase; then centrifuge to collect the precipitate; dialyze the precipitate and freeze-dry to obtain lipase, the enzyme activity of which is determined to be 150,000-250,000 units/g ; Wherein, centrifugation is centrifugation at 4°C, 000g for 8min.

Experimental Principle of Quest Lipase

Quest Lipase from different sources has different catalytic characteristics and catalytic activities. In the project of our team, the experimenters mainly used plant-based lipase (plant-based lipase can cooperate with other enzymes to catalyze and decompose oily substances to produce sugars, which provide the nutrients and energy necessary for seeds to take root and germinate). Compared with animal and microbial fats, vegetable fats are more environmentally friendly and simpler in structure, so they are also the best choice for our team’s project research.

The activity of an enzyme interacting with a substrate is affected by many factors such as temperature, pH value, enzyme solution concentration, substrate concentration, and enzyme activator or inhibitor. The research on the properties of Quest Lipase is also similar, mainly including several aspects such as optimum temperature and pH, temperature and pH stability, substrate specificity, etc. These are also the parts that our team needs to use crude liquid enzyme test in the follow-up experiments .

About Products:
If you need the cosmetic raw material Quest Lipase, please contact our service staff directly, we will provide you with high-quality products at the best price.
As an experienced manufacturer and supplier of Quest Lipase, ZSpharmac will provide Quest Lipase raw materials to customers all over the world for a long time.
Over the years, the company has been adhering to the spirit of “integrity management, strict quality control, customer first”, and has won unanimous praise from domestic and foreign customers.

Company Profile and Corporate Culture

Company Profile:

ZhiShang Chemical is owned by ZhiShang Group is a professional new-type chemicals enterprise combined into research and development, production and sales .

The company’s competitive product is pharmaceutical raw materials and intermediates (especially carbohydrate derivatives Series), In recent years, the company has made a major breakthrough in food and feed additives, plant extraction, industrial chemicals industry .

The company insists on the spirit of “sincere management, strict quality control, customer as god” , get consistent high praise from customers at home and abroad.

Corporate Culture:

OUR MISSION
Help China Chemicals to benefit the happiness of human life
OUR VISSION
Become the most trusted chemical supplier in the world
OUR BUSINESS PHILOSOPHY
Striver – oriented, enrich employees, customer first, deep service, seek development
OUR VALUES
Be prepared for danger in times of peace, forge ahead actively, unity and cooperation, and be brave to fight

About Us

The production base is located in Zhangqiu chemical industry park and Tai’an high-tech chemical industry park. laboratory and workshop in strict accordance with the GMP standard and the product fit national ISO9001 and ISO2000 standards.

“Zhishang” products are exported to Europe, North and South America, the Middle East, Asia Pacific and Africa area, so as to establish a long-term and stable cooperation relationship with customer in the world.

Company Info
  • Business Type: Manufacturer
  • Product Range: Cosmetic raw materials
  • Products/Service:Cosmetic raw materials (flavors and fragrances,antioxidant products, moisturizing and hydrating, whitening agents, cosmetic additives)
  • Total Employees: 51~100
  • Capital (Million US $): 10000000RMB
  • Year Established: 2016
Production Capacity
  • No. of Production Lines : 8
  • No. of QC Staff : 5 -10 People
  • OEM Services Provided : yes
  • Factory Size (Sq.meters) : 3,000-5,000 square meters
  • Certificate: ISO9001 , CE , GMP , API , MSDS
  • Factory Location : Diao Town Industry Park, Zhangqiu City, Jinan City, Shandong Province, China.

Service

Pre-Sales Service

* Prompt reply and 24 hours online, professional team to provide best price and high quality product.

* Sample testing support.

* Every batch of products will be tested to ensureits quality.

*The packing also can be according the customers` requirment.

*Any inquiries will be replied within 24 hours.

*we provide Commerical Invoice, Packing List, Bill of loading, COA , Health certificate and Origin certificate. If your markets have any special requirements, let us know.

 

After-Sales Service

*The fact of logistics information monitoring.

* Any questions about the product can be consulted at any time.

*Product has any problem can return.

FAQ

Do you accept sample order?

We will make samples before mass production, and after sample approved, we’ll begin mass production. Doing 100% inspection during production, then do random inspection before packing.

 

HOW TO CONFIRM THE PRODUCT QUALITY BEFORE PLACING ORDERS?

You can get free samples for some products,you only need to pay the shipping cost or arrange a courier to us and take the samples. You can send us your product specifications and requests,we will manufacture the products according to your requests.

What’s your MOQ?

Our MOQ is 1kg. But usually we accept less quantity such as 100g on the condition that sample charge is 100% paid.

Do you supply product report?

Yes. We’ll give you product analysis report before shipping.

  Is there a discount?

Different quantity has different discount.

Shipping

1. ≤50kg, Express delivery recommended, usually called as DDU service;

2. ≤500kg, Air shipping recommended, usually called as FOB, CFR, or CIF service;

3. >500kg, sea shipping recommended, usually called as FOB, CFR, or CIF service;

4. For high value products, please select air shipping and express delivery for safe.

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