Pure L-Tyrosine CAS 60-18-4

Molecular Formula: C9H11NO3

Formula Weight: 181.19

ZSpharmac: Pure L-Tyrosine Supplier

Product Name: Pure L-Tyrosine

CAS No: 60-18-4

Purity: 99%

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Basic Info

Product Name:	Pure L-Tyrosine
Other Names:	L-Tyrosine
CAS:	60-18-4
Place of Origin:	Shandong, China
Brand Name:	ZSpharmac
Type:	Cosmetic Raw Materials
Appearance:	White to Pale-Brown Powder
EINECS No.:	200-460-4
Storage:	Store Below +30°C.
Provide:	Pure L-Tyrosine MSDS； Pure L-Tyrosine COA

What is Pure L-Tyrosine?

Tyrosine is a non-essential amino acid, which is the raw product of various products in the body. Tyrosine can be traded numerous physiological substances through different metabolic paths in the body, such as dopamine, epinephrine, thyroxine, melanin in addition to papaverine of poppy (opium). These substances are very closely related to the control of nerve transmission as well as also metabolic policy. Looking into tyrosine metabolic rate aids to identify the pathological procedure of particular illness. As an example, alkapsis is connected to tyrosine metabolic procedure problem. The absence of alkaptic acid oxidase in the person’s body produces the failure of the metabolite of tyrosine, alkaptic acid, to continue to be decomposed, and additionally it is removed from the urine as well as additionally oxidized best into a black product when exposed to air. The baby diapers of children with this disease will slowly transform black when revealed to the air, and also the pee will in addition transform black when left for a long period of time. Albinism is also linked to the metabolic rate of tyrosine. Individuals lack tyrosinase to ensure that the tyrosine metabolite 3,4-dihydroxyphenylalanine can not establish melanin, so the hair in addition to skin are white.

Pure L-Tyrosine Property

Melting point	>300 °C (dec.) (lit.)
alpha	-11.65 º (c=5,DIL HCL/H2O 50/50)
Boiling point	314.29°C (rough estimate)
density	1.34
refractive index	-12 ° (C=5, 1mol/L HCl)
Fp	176 °C
storage temp.	Store below +30°C.
solubility	1 M HCl: 25 mg/mL
form	powder
pka	2.2(at 25℃)
color	White to Pale-brown
Odor	odorless
PH	6.5 (0.1g/l, H2O)
Odor Type	odorless
optical activity	[α]20/D 11.5±1.0°, c = 4% in 1 M HCl
Water Solubility	0.45 g/L (25 ºC)

Application of Pure L-Tyrosine

Pure L-Tyrosine is an urine testing agent used to find very early cancer, generally to detect the content of p-hydroxyphenylalanine in pee. Amongst the lump pens, p-hydroxyphenylalanine and also its metabolites are discovered in irregular tumor microorganisms in the urine, so they are made use of as screening markers by public evaluations with incredibly high precision. L-tyrosine is likewise an amino acid made use of in cosmetics to improve the tanning process. When UV rays exist, L-Tyrosine assists enhance melanin production for faster as well as much deeper tanning.

Research studies have revealed that the metabolic problem of Pure L-Tyrosine not only results in the event of various amino acid metabolic illness, but additionally has a specific correlation with deadly tumors. As a result, the study of the web content changes of tyrosine and its metabolites in body liquids is of wonderful importance for the study of supporting medical diagnosis of numerous diseases, effectiveness surveillance and etiology.

According to the existing literary works, the focus of Pure L-Tyrosine in the urine of cancer cells patients will certainly be dramatically more than that of normal individuals, and p-hydroxyphenylalanine as a new tumor pen has actually progressively begun to obtain widespread attention. Today, most of the reported discovery techniques for p-hydroxyphenylalanine are that the discovery reagent is combined with p-hydroxyphenylalanine to develop a particular shade, and compared to the common shade card, a favorable or negative final thought is attracted. This approach can just be made use of for easy qualitative yet not quantitative, and there are lots of disturbance factors in this approach, which is simple to develop incorrect positives or incorrect downsides.

Detection of Pure L-Tyrosine

1. Instruments and medicines

High performance liquid chromatography: Shimadzu NexeraXR, pump: LC-20ADXR, autosampler: SIL-20ACXR, column oven: CTO-20A. Mass spectrometer: ABSCIEXAPI4000, centrifuge: ThermoSORVALLST8R.

Pure L-Tyrosine was purchased from China National Institute of Inspection and Quarantine, and the isotope internal standard solution L-p-hydroxyphenylalanine-D2 was purchased from TLC Company.

2. Chromatographic detection conditions

Chromatographic column: ThermoC183um (2.1×100mm) chromatographic column, the mobile phase A is 0.1% (v/v) formic acid, the B phase is acetonitrile, the flow rate is 0.2-0.3ml/min, the column temperature is 40°C, and the injection volume is 5ul.

3. Preparation of Stock Solution

Preparation of Pure L-Tyrosine stock solution: Weigh 10 mg of p-hydroxyphenylalanine, add ultrapure water with an electric pipette to make the effective concentration 1 mg/ml, and mark it as stock1.

Preparation of L-p-hydroxyphenylalanine-D2 stock solution: Weigh 10 mg of L-p-hydroxyphenylalanine-D2, add ultrapure water with an electric pipette to make the effective concentration 1 mg/ml, and mark it as stock2.

4. Preparation of Standard Curve Solution

Dilute the stock solution stock1 with ultrapure water step by step to 2000ng/ml, 1000ng/ml, 500ng/ml, 100ng/ml, 50ng/ml, 10ng/ml, 5ng/ml, 1ng/ml, respectively take 190ul of the above-mentioned solutions of each concentration, add stock solution Stock210ul, mix well, and obtain the standard curve solution of p-hydroxyphenylalanine.

5. Preparation of urine samples to be tested

Take 1ml of human urine sample, centrifuge at 1500×g/min for 10-15min, take the supernatant, dilute it 50 times, pass through a 0.22um microporous membrane, take 190ul filtrate and add 10ul stock solution Stock2, mix well.

6. Linear analysis

Take the standard curve solution of p-hydroxyphenylalanine obtained in step 4 for liquid phase mass spectrometry detection, and use liquid chromatography-mass spectrometer for detection. The chromatographic detection conditions of Pure L-Tyrosine are the conditions described in step 2. Linear regression analysis was performed with the theoretical value of the corrected concentration in the standard curve as the abscissa and the area ratio of p-hydroxyphenylalanine to the isotope internal standard as the ordinate. The regression curve equation is y=4.31e+003x+1.53e+004 (r=0.9991), and the weight coefficient is 1/x2.

7. Testing of urine samples to be tested

Select 200 urine samples prepared in step 5 for liquid chromatography-mass spectrometry detection, and use liquid chromatography-mass spectrometry for detection. The detection results are brought into the standard curve equation in step 6 to obtain the content of p-hydroxyphenylalanine in the urine sample. Among them, the maximum value is 39407ng/ml; the minimum value is 1260ng/ml; the average value is 11088ng/ml, and the positive rate is 6%.

Production Method of Pure L-Tyrosine

The prep work of L-tyrosine mostly embraces the protein hydrolysis extraction method. Basic material such as pig blood dish, horned unguis, and also silk can be used, hydrolyzed with acid, and afterwards separated and detoxified.
Pig blood meal [HCl (hydrolysis)] → [110 ℃, 24h] hydrolyzate [acid elimination] → [evaporative concentration] acid removal solution [turned on carbon] → decolorization remedy [decolorization, cooling and crystallization] → L-tyrosine unrefined item [activated carbon (fine-tuned)] → [90 ℃,30 min] filtrate [condensation] → L-tyrosine.
Hydrolysis and also acid elimination Put pig blood meal, water, and also industrial hydrochloric acid right into the hydrolysis tank at a weight ratio of 1:1.3:1, warm to 112-114 ° C, quit mixing and also reflux for 24 hr, amazing and also filter to eliminate, as well as obtain the filtrate, which is the hydrolyzate. Vaporize as well as focus the hydrolyzate to a syrupy state, include water to dissolve and vaporize to concentrate, as well as repeat this three times to catch acid.
Add pure water to water down the decolorized as well as taken shape focused solution till it is completely liquified, then add heavy ammonia water to readjust the pH value of the solution to 3.5, include 1% triggered carbon, stir as well as steam for 10 mins, mix and warm in a water bath at 90 ° C for 30 minutes, filter while hot, clean the turned on carbon layer with distilled water for 3 times, as well as combine the filtrate and also cream. According to this technique, continue to decolorize with triggered carbon till the solution is light yellow. The filtrate was put listed below 10 ° C for 1 day, and also crystals were precipitated, as well as the unrefined product of L-tyrosine was gotten by purification.
Recrystallization and also refining Include the crude tyrosine to distilled water at a ratio of 1:20, let it dissolve completely, include 1% activated carbon, maintain mixing at 90 ° C for half an hour, filter while it is hot, and awesome and also refine the filtrate to precipitate crystals. The crystals were collected by filtering, cleaned two times with outright ethanol, and also dried at 60 ° C to acquire the finished item of L-tyrosine.

About Products:
If you need Pure L-Tyrosine as a cosmetic raw material, please contact our service staff directly, we will provide you with high-quality products at the best price.
As an experienced Pure L-Tyrosine manufacturer and supplier, ZSpharmac will provide Pure L-Tyrosine raw materials for customers all over the world for a long time.
Over the years, the company has been adhering to the spirit of “integrity management, strict quality control, customer first”, and has won unanimous praise from domestic and foreign customers.

Company Profile and Corporate Culture

Company Profile：

ZhiShang Chemical is owned by ZhiShang Group is a professional new-type chemicals enterprise combined into research and development, production and sales .

The company’s competitive product is pharmaceutical raw materials and intermediates (especially carbohydrate derivatives Series), In recent years, the company has made a major breakthrough in food and feed additives, plant extraction, industrial chemicals industry .

The company insists on the spirit of “sincere management, strict quality control, customer as god” , get consistent high praise from customers at home and abroad.

Corporate Culture：

OUR MISSION
Help China Chemicals to benefit the happiness of human life
OUR VISSION
Become the most trusted chemical supplier in the world
OUR BUSINESS PHILOSOPHY
Striver – oriented, enrich employees, customer first, deep service, seek development
OUR VALUES
Be prepared for danger in times of peace, forge ahead actively, unity and cooperation, and be brave to fight

About Us

The production base is located in Zhangqiu chemical industry park and Tai’an high-tech chemical industry park. laboratory and workshop in strict accordance with the GMP standard and the product fit national ISO9001 and ISO2000 standards.

“Zhishang” products are exported to Europe, North and South America, the Middle East, Asia Pacific and Africa area, so as to establish a long-term and stable cooperation relationship with customer in the world.

Company Info

Business Type: Manufacturer
Product Range: Cosmetic raw materials
Products/Service:Cosmetic raw materials (flavors and fragrances,antioxidant products, moisturizing and hydrating, whitening agents, cosmetic additives)
Total Employees: 51~100
Capital (Million US $): 10000000RMB
Year Established: 2016

Production Capacity

No. of Production Lines : 8
No. of QC Staff : 5 -10 People
OEM Services Provided : yes
Factory Size (Sq.meters) : 3,000-5,000 square meters
Certificate: ISO9001 , CE , GMP , API , MSDS
Factory Location : Diao Town Industry Park, Zhangqiu City, Jinan City, Shandong Province, China.

Service

Pre-Sales Service

* Prompt reply and 24 hours online, professional team to provide best price and high quality product.

* Sample testing support.

* Every batch of products will be tested to ensureits quality.

*The packing also can be according the customers` requirment.

*Any inquiries will be replied within 24 hours.

*we provide Commerical Invoice, Packing List, Bill of loading, COA , Health certificate and Origin certificate. If your markets have any special requirements, let us know.

After-Sales Service

*The fact of logistics information monitoring.

* Any questions about the product can be consulted at any time.

*Product has any problem can return.

FAQ

Do you accept sample order?

We will make samples before mass production, and after sample approved, we’ll begin mass production. Doing 100% inspection during production, then do random inspection before packing.

HOW TO CONFIRM THE PRODUCT QUALITY BEFORE PLACING ORDERS?

You can get free samples for some products,you only need to pay the shipping cost or arrange a courier to us and take the samples. You can send us your product specifications and requests,we will manufacture the products according to your requests.

What’s your MOQ?

Our MOQ is 1kg. But usually we accept less quantity such as 100g on the condition that sample charge is 100% paid.

Do you supply product report?

Yes. We’ll give you product analysis report before shipping.

Is there a discount?

Different quantity has different discount.

Shipping

1. ≤50kg, Express delivery recommended, usually called as DDU service;

2. ≤500kg, Air shipping recommended, usually called as FOB, CFR, or CIF service;

3. >500kg, sea shipping recommended, usually called as FOB, CFR, or CIF service;

4. For high value products, please select air shipping and express delivery for safe.