Inhibitory effect on lung cancer
Experimental studies have shown that Pure Honokiol can inhibit the growth of non-small cell lung cancer cells in a concentration-dependent manner in vitro and induce their apoptosis; Singh et alshowed that HNK can also target PGE2-activated β- Catenin signaling inhibits the invasion of non-small cell lung cancer; another study reported that HNK can inhibit the proliferation of lung squamous cell carcinoma cells, induce cell cycle arrest in G1-S phase, and induce cell apoptosis, and can significantly inhibit NSCLC. -nitroso-trischloroethylurea-induced generation of mouse broncho-lung squamous cell carcinoma, the inhibitory effect was statistically significant (P = 0.004).
Inhibitory effect on gastrointestinal tumors
Pure Honokiol can inhibit the proliferation of colorectal cancer cells RKO, SW480 and LS180, and induce the appearance of DNA fragment ladders in RKO cells. Flow cytometry detection shows that HNK can induce apoptosis in RKO cells, and HNK-induced apoptosis in this colon cancer cell Does not have p53-dependent properties. HNK can also inhibit the growth of RKO tumors transplanted in nude mice and prolong the survival of nude mice bearing RKO tumors . Therefore, HNK can inhibit the growth of colon cancer both in vivo and in vitro.
Inhibitory effect on breast cancer
Pure Honokiol can inhibit the proliferation of various breast cancer cells such as MCF-7, SK-BR3 and BT-474 in vitro, and its half effective inhibitory concentration (IC50) value is between 10 and 70 μmol∙L-1 If breast cancer cells SK-BR3 were inoculated into nude mice, HNK was administered intraperitoneally at a dose of 100 mg&# 8729; kg-1 for a total of 4 weeks, HNK could basically inhibit the growth of tumors, and the administration for 2 weeks There was a significant difference (P < 0.02) in the tumor size when compared with the control group. HNK induced apoptosis of MCF-7 cells in vitro, leading to cut bands of PARP and caspase-8, arresting cells in G1-S phase, inhibiting the expression of cyclin D1, and up-regulating the expression of p27 and p21 . HNK inhibits the invasion and metastasis of breast cancer cells MCF-7 and MDA-MB-231 by inhibiting epithelial-mesenchymal transition, thereby producing anti-breast cancer effects .
Inhibitory effect on ovarian cancer
Ovarian cancer is one of the most common female genital tumors, and its 5-year survival rate is 20% to 30%. It is of practical significance to study drugs for the treatment of ovarian cancer. Pure Honokiol can inhibit the proliferation of ovarian cancer cells such as SKOV3, Coc1, Angelen and A2780 in vitro, and induce SKOV3 and Coc1 cells to produce DNA fragment ladders and cell cycle arrest in G0/G1 phase. HNK was administered intraperitoneally at 50 mg∙kg-1, which could significantly inhibit the growth of transplanted tumors in SKOV3 nude mice, and at the same time reduce the expression of vascular endothelial growth factor (VEGF) and microvessel density in tumor tissues. HNK may produce anti-ovarian cancer effects by inducing tumor cell apoptosis, cell cycle arrest and inhibiting tumor angiogenesis.
Inhibitory effect on prostate cancer
Pure Honokiol can inhibit the proliferation of prostate cancer cells PC-3 and LNCaP in a time- and dose-dependent manner, and cause LNCaP cell cycle arrest in G0-G1 phase, reduce cell cycle-dependent proteins cyclin D1 and cyclin-dependent kinase 4 (cyclin-dependent kinase 4, CDK4), CDK6 and cyclin E expression, and inhibit the formation of cyclin D1 and CDK4 complex, can reduce the protein level and phosphorylation level of Rb (retinoblastoma protein), thus causing cell cycle arrest . HNK can also induce autophagy in PC-3, LNCaP and Myc-CaP prostate cancer cells, and the autophagy inhibitor 3-methyladenine (3-MA) can promote the inhibitory effect of HNK on the proliferation of prostate cancer cells Autophagy may play a protective role in the anti-tumor effect of HNK on prostate cancer, so inhibition of autophagy can promote the anti-tumor effect of HNK.
Inhibitory effect on head and neck squamous cell carcinoma
Head and neck squamous cell carcinoma is one of the most common tumors in the world. Pure Honokiol inhibits the proliferation of head and neck tumor cells such as SCC-1, SCC-5, OSC-19 and FaDu in vitro, and this proliferation inhibition is time- and concentration-dependent; at the same time, HNK can also induce SCC-1 and FaDu Apoptosis, decreased expression of Bcl-2 and increased expression of Bax. When HNK was orally administered at 100 mg∙kg-1 body weight to nude mice inoculated with SCC-1 and FaDu, HNK significantly inhibited the growth of the two transplanted tumors. HNK has growth inhibitory effect on head and neck squamous cell carcinoma both in vivo and in vitro.
Inhibitory effect on other tumors
Pure Honokiol can also inhibit the growth and proliferation of brain tumors, leukemia and skin cancer cells, so it has a wide range of anti-tumor effects.