Pure Honokiol CAS 35354-74-6

Molecular Formula: C18H18O2

Formula Weight: 266.33

ZSpharmac: Pure Honokiol Supplier

Product Name: Pure Honokiol

CAS No: 35354-74-6

Purity: 99%

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Basic Info

Product Name:	Pure Honokiol
Other Names:	Honokiol
CAS:	35354-74-6
Place of Origin:	Shandong, China
Brand Name:	ZSpharmac
Type:	Cosmetic Raw Materials
Appearance:	White to Off-White Powder
EINECS No.:	609-119-8
Storage:	2-8°C
Provide:	Pure Honokiol MSDS； Pure Honokiol COA

What is Pure Honokiol?

Magnolia officinalis, also referred to as Xiangpu, Chipu, Ziyou Magnolia, etc, is a generally made use of standard Chinese medicine. Very first videotaped in “Shen Nong’s Materia Medica”, provided as middle quality.
Pure Honokiol is an item removed from the dried origin bark, stem bark or branch bark of Magnolia officinalis, etc, and the business essences are generally standard for the content of magnolol and also honokiol.
[Distribution] There are about 90 species of Magnolia plants on the planet, and concerning 30 species in my nation, of which about 20 varieties have medicinal value. Magnolia officinalis grows in bright hills as well as woodland margins with productive soil and also deep dirt at an elevation of 300-1700m.
[Origin] Magnolia officinalis is generally dispersed in western Hubei, southern Sichuan, southerly Shaanxi as well as southerly Gansu; Magnolia officinale is primarily dispersed in Jiangxi, Anhui, Zhejiang, Fujian, Hunan, Guangxi as well as northern Guangdong. Magnolia officinalis and Magnolia officinale are primarily distributed in a cross, and also both have massive man-made farming.
[Main ingredients] Magnolia bark consists of magnolol, honokiol, isomagolol and also other components. As well as isolated trihydroxy magnolol, deoxy trihydroxy magnolol, trihydroxy magnoloaldehyde, poly magnolol An as well as C from the ethyl acetate essence of Magnolia officinalis. The bark contains concerning 1% unpredictable oil, as well as the oil mainly contains β-oleyl alcohol; it likewise has α-pinene, β-pinene, limonene, and bornyl acetate. Magnolia curine is additionally had in the bark. Leaves also consist of magnolol and also honokiol.
[Collecting and also processing] From April to June, the completely dry bark of trees that have grown for more than 15 years is removed, boiled in boiling water, piled up in an earth pit, covered with yard to make it “sweat”, and also after the water seeps out from the within, When the internal surface area transforms purple-brown or tan, heavy steam once more till soft, take it out, roll it into a tube, as well as completely dry it in the sunlight or on a kang. After the root bark and also branch bark are removed, they can be dried out straight in the color.

Pure Honokiol Property

Melting point	86℃
Boiling point	400.1±40.0 °C(Predicted)
density	1.107±0.06 g/cm3(Predicted)
storage temp.	2-8°C
solubility	DMSO: 36 mg/mL
form	powder
pka	9.89±0.48(Predicted)
color	White to Off-White

How Pure Honokiol Works

Inhibitory effect on lung cancer
Experimental studies have shown that Pure Honokiol can inhibit the growth of non-small cell lung cancer cells in a concentration-dependent manner in vitro and induce their apoptosis; Singh et alshowed that HNK can also target PGE2-activated β- Catenin signaling inhibits the invasion of non-small cell lung cancer; another study reported that HNK can inhibit the proliferation of lung squamous cell carcinoma cells, induce cell cycle arrest in G1-S phase, and induce cell apoptosis, and can significantly inhibit NSCLC. -nitroso-trischloroethylurea-induced generation of mouse broncho-lung squamous cell carcinoma, the inhibitory effect was statistically significant (P = 0.004).

Inhibitory effect on gastrointestinal tumors
Pure Honokiol can inhibit the proliferation of colorectal cancer cells RKO, SW480 and LS180, and induce the appearance of DNA fragment ladders in RKO cells. Flow cytometry detection shows that HNK can induce apoptosis in RKO cells, and HNK-induced apoptosis in this colon cancer cell Does not have p53-dependent properties. HNK can also inhibit the growth of RKO tumors transplanted in nude mice and prolong the survival of nude mice bearing RKO tumors . Therefore, HNK can inhibit the growth of colon cancer both in vivo and in vitro.

Inhibitory effect on breast cancer
Pure Honokiol can inhibit the proliferation of various breast cancer cells such as MCF-7, SK-BR3 and BT-474 in vitro, and its half effective inhibitory concentration (IC50) value is between 10 and 70 μmol∙L-1 If breast cancer cells SK-BR3 were inoculated into nude mice, HNK was administered intraperitoneally at a dose of 100 mg&# 8729; kg-1 for a total of 4 weeks, HNK could basically inhibit the growth of tumors, and the administration for 2 weeks There was a significant difference (P < 0.02) in the tumor size when compared with the control group. HNK induced apoptosis of MCF-7 cells in vitro, leading to cut bands of PARP and caspase-8, arresting cells in G1-S phase, inhibiting the expression of cyclin D1, and up-regulating the expression of p27 and p21 . HNK inhibits the invasion and metastasis of breast cancer cells MCF-7 and MDA-MB-231 by inhibiting epithelial-mesenchymal transition, thereby producing anti-breast cancer effects .

Inhibitory effect on ovarian cancer
Ovarian cancer is one of the most common female genital tumors, and its 5-year survival rate is 20% to 30%. It is of practical significance to study drugs for the treatment of ovarian cancer. Pure Honokiol can inhibit the proliferation of ovarian cancer cells such as SKOV3, Coc1, Angelen and A2780 in vitro, and induce SKOV3 and Coc1 cells to produce DNA fragment ladders and cell cycle arrest in G0/G1 phase. HNK was administered intraperitoneally at 50 mg∙kg-1, which could significantly inhibit the growth of transplanted tumors in SKOV3 nude mice, and at the same time reduce the expression of vascular endothelial growth factor (VEGF) and microvessel density in tumor tissues. HNK may produce anti-ovarian cancer effects by inducing tumor cell apoptosis, cell cycle arrest and inhibiting tumor angiogenesis.

Inhibitory effect on prostate cancer
Pure Honokiol can inhibit the proliferation of prostate cancer cells PC-3 and LNCaP in a time- and dose-dependent manner, and cause LNCaP cell cycle arrest in G0-G1 phase, reduce cell cycle-dependent proteins cyclin D1 and cyclin-dependent kinase 4 (cyclin-dependent kinase 4, CDK4), CDK6 and cyclin E expression, and inhibit the formation of cyclin D1 and CDK4 complex, can reduce the protein level and phosphorylation level of Rb (retinoblastoma protein), thus causing cell cycle arrest . HNK can also induce autophagy in PC-3, LNCaP and Myc-CaP prostate cancer cells, and the autophagy inhibitor 3-methyladenine (3-MA) can promote the inhibitory effect of HNK on the proliferation of prostate cancer cells Autophagy may play a protective role in the anti-tumor effect of HNK on prostate cancer, so inhibition of autophagy can promote the anti-tumor effect of HNK.

Inhibitory effect on head and neck squamous cell carcinoma
Head and neck squamous cell carcinoma is one of the most common tumors in the world. Pure Honokiol inhibits the proliferation of head and neck tumor cells such as SCC-1, SCC-5, OSC-19 and FaDu in vitro, and this proliferation inhibition is time- and concentration-dependent; at the same time, HNK can also induce SCC-1 and FaDu Apoptosis, decreased expression of Bcl-2 and increased expression of Bax. When HNK was orally administered at 100 mg∙kg-1 body weight to nude mice inoculated with SCC-1 and FaDu, HNK significantly inhibited the growth of the two transplanted tumors. HNK has growth inhibitory effect on head and neck squamous cell carcinoma both in vivo and in vitro.

Inhibitory effect on other tumors
Pure Honokiol can also inhibit the growth and proliferation of brain tumors, leukemia and skin cancer cells, so it has a wide range of anti-tumor effects.

Biological Activity of Pure Honokiol

Honokiol (HNK) is just one of the major energetic components of the typical Chinese medicine Magnolia officinalis, which has a wide range of biological impacts, such as moderate antitumor task. Honokiol can hinder the development of lung cancer, gastrointestinal tumors, head and neck squamous cell cancer, bust cancer, prostate cancer and also ovarian cancer cells artificial insemination as well as in vivo. It has a vast array of targets, can manage apoptosis-related signaling paths, and hinder development Factor-mediated signaling pathways, obstructing NF-κB signaling paths, decreasing the expression of androgen receptor, preventing mTOR as well as STAT3 signaling paths, and so on. HNK can raise the anti-tumor result of standard anti-tumor medicines or targeted anti-tumor drugs, and turn around the drug resistance of tumor cells to cisplatin, doxorubicin and paclitaxel. It can be seen that the combination of honokiol as well as antineoplastic medicines has the impact of synergizing and also reversing medicine resistance, as well as it is made use of scientifically as a biochemical regulator.

Preparation Method of Pure Honokiol

The first step: Take 14.80 g of 4-allyl anisole in a 250 ml flask, slowly add an appropriate amount of pyridine perbromide as a bromination agent, and add an appropriate amount of Zn as a catalyst, and react for 3 h. The fraction at 152°C was distilled under reduced pressure, which was 2-bromo-4-allyl anisole.

Step 2: Take 18.70 g of 4-bromo-anisole, add ethylmagnesium chloride and 2,5-dimethylphenylmagnesium bromide, react at 40°C for 2 hours, then slowly add 12.10 g of allyl bromide, After reacting for 5 hours, the fraction at 150°C was distilled under reduced pressure, which was 4-bromo-2-allyl anisole.

Step 3: Dissolve 2.38 g of nickel disilicide hexahydrate in 2 ml of water, add 5.25 g of triphenylphosphine, dissolve in a solution of 75 ml of glacial acetic acid, react at room temperature for 24 h, and olive green microcrystals are precipitated in the reaction solution. Filter, wash with glacial acetic acid, and place in a sulfuric acid or potassium hydroxide desiccator to dry in vacuum to obtain dark blue crystals.

Step 4: Take 150 ml of anhydrous ether in a 250 ml flask, add 3.00 g of magnesium strips, soak for 1 h, then slowly add the prepared 4-bromo-2-allyl anisole, and react 3 h, make 4-bromo-2-allyl anisole into Grignard reagent, then add the prepared 2-bromo-4-allyl anisole to it, react for 12 h, distill out under reduced pressure Fractions below 150°C were used to obtain honokiol methyl ether, then hydrolyzed, finally extracted with ethyl acetate and water, concentrated to dryness under reduced pressure, and purified by silica gel column chromatography (ether: hexane = 1:2) to obtain The white crystalline product is Pure Honokiol.

About Products:
If you need Pure Honokiol, the cosmetic raw material, please contact our service staff directly, we will provide you with high-quality products at the best price.
As an experienced Pure Honokiol manufacturer and supplier, ZSpharmac will provide Pure Honokiol raw materials to customers all over the world for a long time.
Over the years, the company has been adhering to the spirit of “integrity management, strict quality control, customer first”, and has won unanimous praise from domestic and foreign customers.

Company Profile and Corporate Culture

Company Profile：

ZhiShang Chemical is owned by ZhiShang Group is a professional new-type chemicals enterprise combined into research and development, production and sales .

The company’s competitive product is pharmaceutical raw materials and intermediates (especially carbohydrate derivatives Series), In recent years, the company has made a major breakthrough in food and feed additives, plant extraction, industrial chemicals industry .

The company insists on the spirit of “sincere management, strict quality control, customer as god” , get consistent high praise from customers at home and abroad.

Corporate Culture：

OUR MISSION
Help China Chemicals to benefit the happiness of human life
OUR VISSION
Become the most trusted chemical supplier in the world
OUR BUSINESS PHILOSOPHY
Striver – oriented, enrich employees, customer first, deep service, seek development
OUR VALUES
Be prepared for danger in times of peace, forge ahead actively, unity and cooperation, and be brave to fight

About Us

The production base is located in Zhangqiu chemical industry park and Tai’an high-tech chemical industry park. laboratory and workshop in strict accordance with the GMP standard and the product fit national ISO9001 and ISO2000 standards.

“Zhishang” products are exported to Europe, North and South America, the Middle East, Asia Pacific and Africa area, so as to establish a long-term and stable cooperation relationship with customer in the world.

Company Info

Business Type: Manufacturer
Product Range: Cosmetic raw materials
Products/Service:Cosmetic raw materials (flavors and fragrances,antioxidant products, moisturizing and hydrating, whitening agents, cosmetic additives)
Total Employees: 51~100
Capital (Million US $): 10000000RMB
Year Established: 2016

Production Capacity

No. of Production Lines : 8
No. of QC Staff : 5 -10 People
OEM Services Provided : yes
Factory Size (Sq.meters) : 3,000-5,000 square meters
Certificate: ISO9001 , CE , GMP , API , MSDS
Factory Location : Diao Town Industry Park, Zhangqiu City, Jinan City, Shandong Province, China.

Service

Pre-Sales Service

* Prompt reply and 24 hours online, professional team to provide best price and high quality product.

* Sample testing support.

* Every batch of products will be tested to ensureits quality.

*The packing also can be according the customers` requirment.

*Any inquiries will be replied within 24 hours.

*we provide Commerical Invoice, Packing List, Bill of loading, COA , Health certificate and Origin certificate. If your markets have any special requirements, let us know.

After-Sales Service

*The fact of logistics information monitoring.

* Any questions about the product can be consulted at any time.

*Product has any problem can return.

FAQ

Do you accept sample order?

We will make samples before mass production, and after sample approved, we’ll begin mass production. Doing 100% inspection during production, then do random inspection before packing.

HOW TO CONFIRM THE PRODUCT QUALITY BEFORE PLACING ORDERS?

You can get free samples for some products,you only need to pay the shipping cost or arrange a courier to us and take the samples. You can send us your product specifications and requests,we will manufacture the products according to your requests.

What’s your MOQ?

Our MOQ is 1kg. But usually we accept less quantity such as 100g on the condition that sample charge is 100% paid.

Do you supply product report?

Yes. We’ll give you product analysis report before shipping.

Is there a discount?

Different quantity has different discount.

Shipping

1. ≤50kg, Express delivery recommended, usually called as DDU service;

2. ≤500kg, Air shipping recommended, usually called as FOB, CFR, or CIF service;

3. >500kg, sea shipping recommended, usually called as FOB, CFR, or CIF service;

4. For high value products, please select air shipping and express delivery for safe.