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Protocatechuic Acid CAS 99-50-3

Molecular Formula: C7H6O4

Formula Weight: 154.12

ZSpharmac: Protocatechuic Acid Supplier

Product Name: Protocatechuic Acid
CAS No: 99-50-3
Purity: 99%

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Basic Info

Product Name:Protocatechuic Acid
Other Names:3,4-Dihydroxybenzoic Acid
Place of Origin:Shandong, China
Brand Name:ZSpharmac
Type:Cosmetic Raw Materials
Appearance:Beige to Brown Fine Crystalline Powder
EINECS No.:202-760-0
Storage:Store Below +30°C.
Provide:Protocatechuic Acid MSDS;
Protocatechuic Acid COA

What is Protocatechuic Acid?

Protocatechuic acid (PCA) is 3,4-dihydroxybenzoic acid. is an easy phenolic acid that is a forerunner for the synthesis of various other complicated particles such as anthocyanin 3-O-β-D-glucoside and also vanillin. It is uncovered in edible veggies, fruits, nuts, brown rice, pecans, tea, hibiscus blossoms, as well as additionally some organic medications.Protocatechuic acid has a range of biological activities versus various molecular targets. It has anti-bacterial, anti-oxidant, anti-inflammatory, anti-hyperglycemic as well as neuroprotective results. Additionally, protocatechuic acid has potential chemical defense. It can prevent chemical health hazards artificial insemination and produce pro-apoptotic and also anti-proliferative effects in different facets. As a result, it is currently utilized medically for the therapy of burns, childish pneumonia, bacillary dysentery, acute pyelonephritis, intense pancreatitis as well as certain abscess illness.

Protocatechuic Acid Property

Melting point 

197-200 °C (dec.) (lit.)

Boiling point 

237.46°C (rough estimate)



refractive index 

1.6400 (estimate)

storage temp. 

Store below +30°C.




4.48(at 25℃)


Fine Crystalline Powder


Beige to brown


3.78(1 mM solution);3.25(10 mM solution);2.74(100 mM solution)

Water Solubility 

Soluble in hot alcohol and ether. Slightly soluble in water.



Protocatechuic Acid Pharmacological Action

Protocatechuic acid is a metabolite of intricate polyphenols, such as anthocyanins as well as proanthocyanidins, which are abundant in plants as well as fruits and also can be taken in by pets as well as human beings. It is estimated that individuals take in more anthocyanins than various other Polyphenols must be high, so the dietary value of protocatechuic acid is an increasing number of recognized. A a great deal of experiments have actually proved that protocatechuic acid has a range of biological tasks versus different molecular targets, and it has antibacterial, anti-oxidant, anti-inflammatory, anti-hyperglycemic and also neuroprotective impacts. It can prevent chemical health hazards artificial insemination and generate pro-apoptotic as well as anti-proliferative effects in various aspects.

1. Anti-bacterial impact of protocatechuic acid

Protocatechuic acid has a large range of antibacterial impacts. In vitro experiments, protocatechuic acid separated from the plant roselle has a solid antibacterial impact on methicillin-resistant Staphylococcus aureus, Klebsiella pneumoniae, and also Pseudomonas aeruginosa. And Acinetobacter baumannii has repressive impact, and also the anti-bacterial capability of protocatechuic acid is much better than that of roselle, and the heat-treated protocatechuic acid does not impact its antibacterial activity. Protocatechuic acid can be contributed to muscle mass foods to avoid Campylobacter contamination and to delay lipid oxidation. When the minimum repressive focus of protocatechuic acid is 24-44microg/ mL, it can properly prevent food putridity germs, Salmonella typhimurium DT104, Escherichia coli O157: H7, Listeria monocytogenes, Staphylococcus aureus, as well as Bacillus cereus bacilli, and revealed a dose-dependent manner, which indicated that protocatechuic acid might be used as an artificial additive to stop bacterial contamination.

2. Anticancer effect of protocatechuic acid

Protocatechuic acid has a particular role in cancer cells avoidance, and also its device of activity might be related to its antioxidant task, consisting of hindering development, scavenging oxygen complimentary radicals, and also up-regulating enzymes involved in neutralization, and so on, or it affects the first phase and also stage of some health hazards. The second stage of metabolic process might also straight obstruct the binding site of the final carcinogen via the DNA molecule, thereby protecting against the development of compounds that create mutation and also growth improvement, and also play a particular role in avoiding cancer cells. Protocatechuic acid drawn out from honeysuckle can successfully eliminate HepG2 liver cancer cells by causing JNK-dependent apoptosis of liver cancer cells at 100 micromol/L. Protocatechuic acid can inhibit the metabolism of cancer cells as well as down-regulate the Ras/Akt/NF- κB path by triggering RhoB, which in turn brings about a decline in the activity of MMP-induced cancer cells as well as minimizes the invasiveness of cancer cells. It has certain worth in the growth of anti-cancer. Protocatechuic acid has a pro-apoptotic result on human breast, lung, liver, cervix, and prostate cancer cells, and also reduces the stability of these cancer cells in a concentration-dependent manner. It is a possible anticancer representative by preventing the apoptosis of lump cells or postponing the seepage as well as metabolic process of lump cells to exert its anticancer result.

3. Antioxidant result of protocatechuic acid

Alpinia protocatechuic acid reduces hydrogen peroxide-induced cell survival in vitro, avoids oxidative damages, and also relieves oxidative stress and anxiety in vivo. Its protective impact may be through anti-peroxidase task and also restraint of Generated by complimentary radicals. Hibiscus protocatechuic acid is protective against t-BHP-induced oxidative damage in hepatocytes. Protocatechuic acid has a safety impact on dioxin-induced oxidative damages and also histopathological damages of rat heart tissue, by enhancing the thiobarbituric acid active substance in rat heart cells induced by dioxin, Lower the web content of glutathione, catalase, glutathione peroxidase and superoxide dismutase, consequently reducing death and also hemorrhage of heart tissue.

4. Anti-inflammatory result of protocatechuic acid

Protocatechuic acid has an anti-inflammatory result, and also it has a safety impact on LPS-induced RAW-264.7 cells and the air bag version of computer mice generated by carrageenan, and also can minimize pro-inflammatory elements TNF-α, IL-1β, as well as advertise swelling Conciliators NO, PGE2, INOS, COX-2, NF-κB and MAPK. On top of that, protocatechuic acid is a crucial energetic ingredient in Sijiqing tablet computers, which are made use of as anti-bacterial and also anti-inflammatory drugs.

5. Anti-hyperglycemic effect of protocatechuic acid

In diabetic person mice, protocatechuic acid significantly decreased blood glucose degrees as well as enhanced insulin levels. The reason might be that protocatechuic acid reduces the complications of diabetes, reduces triglycerides, as well as exerts its antioxidant as well as anti-inflammatory effects. Protocatechuic acid is beneficial to avoid or reduce the associated glycation of diabetic person problems.

6. Various other results of protocatechuic acid

In 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine-induced computer mice, protocatechuic acid minimized the levels of dopamine and also dopamine metabolites in the striatum, Hindering this neurotoxicity, improving the pathology of the substantia nigra, decreasing the expression of tyrosine hydroxylase in the substantia nigra, thus playing a certain role in neuroprotection, it might be utilized as a treatment for Parkinson’s illness scientifically. Protocatechuic acid has potential anti-infectious bursal infection. Protocatechuic acid can effectively lower atherosclerosis in apolipoprotein E-deficient mice. Protocatechuic acid stops dioxin-induced reproductive poisoning in male rats.

Extraction Method of Protocatechuic Acid

1. Extraction of protocatechuic acid from lilac leaves

(1) Pretreatment of samples. The original medicinal material of lilac leaves is dried and selected to remove seeds and various sundries, and pulverized by a pulverizer to obtain a fine powder.

(2) Protocatechuic acid extraction. Weigh 2.0 g of lilac leaf powder passed through a 40-mesh sieve, and extract with absolute ethanol as the extractant under the set process conditions. The extract was filtered, the dregs were discarded, and the ethanol was recovered to obtain a crude extract. Add 50ml of ether and 3ml of 0.1mol/L hydrochloric acid to the crude extract, shake to dissolve it, transfer it to a separatory funnel, and extract 3 times with 0.1mol/L sodium hydroxide solution, 50ml each time, and the hydrogen after each extraction After washing the sodium oxide solution with 10ml of ether, add 3ml of 1mol/L hydrochloric acid, combine the sodium hydroxide solution, and then extract with 50ml of ether for 3 times, wash the ether layer extracted three times with 10ml of water, combine the ether solution, and adjust the pH to 6.0 , and evaporated to dryness, almost white powdery crystals were obtained.

2. Extraction of protocatechuic acid from thistle

Add 100g of thistle crude drug (dry basis) to 1000ml of water and decoct for 0.5h, combine the water decoction twice, filter with suction, discard the filter residue, the filtrate is brown, heat and concentrate in an electric furnace to 800ml, extract with ethyl acetate after cooling, and retain the ester Layer and water layer (I) are used to extract flavonoids and caffeic acid. Concentrate the ester layer to dryness under reduced pressure, extract with hot water, filter with suction, discard the water-insoluble matter, acidify the aqueous solution and extract with ether, discard the water layer, and keep the ether layer. The ether layer was extracted with 5% sodium bicarbonate solution, the ether layer was discarded, and the sodium bicarbonate extract was retained. Acidify the sodium bicarbonate extract, extract with ether, adjust the pH value to 6, concentrate and crystallize, and recrystallize with CHCl3 to obtain colorless needle-shaped protocatechuic acid crystals. The melting point of the product is 198-200°C.

The Preparation Method of Protocatechuic Acid

Utilizing piperonal as basic material, initial oxidizing with potassium permanganate to create piperic acid, and after that hydrolyzing with light weight aluminum trichloride to prepare protocatechuic acid, the synthetic route is as complies with:


Synthetic course map of protocatechuic acid
Fig. 1 is the synthetic course layout of protocatechuic acid

1. Prep work of piperic acid
Include 12 g of piperonal and 300 ml of water into a 1000 ml three-necked flask, heat to 70-80 ° C, and also include 5% potassium permanganate remedy dropwise under energetic stirring (18 g potassium permanganate plus 360 ml water), the dropwise addition time has to do with 30 min, after the addition is complete, continue to mix up until the purple shade discolors, filter while warm, and also wash the filter cake. The mom liquor was acidified, filtered to get piperic acid, and also 12.4 g of the completed product was naturally dried, with a yield of 94% and a melting point of 228-231 ° C.
2. Preparation of Protocatechuic Acid
Include 4 g of lightweight light weight aluminum trichloride to 150 ml of nitrobenzene, mix to liquify, include 10 g of piperic acid in collections, mix at location temperature level for 3 to 5 h, put the response service right into 100 ml of water, different the water layer, in addition to in addition placed Sulfur dioxide was entered the aqueous solution, along with protocatechuic acid was precipitated. The totally completely dry weight is 7.5 g, the melting point is 200-201 ° C, as well as the return is 81%.

About Products:
If you need Protocatechuic Acid, a cosmetic raw material, please contact our service staff directly, and we will provide you with high-quality products at the best price.
As an experienced Protocatechuic Acid manufacturer and supplier, ZSpharmac will provide Protocatechuic Acid raw materials to customers all over the world for a long time.
Over the years, the company has been adhering to the spirit of “integrity management, strict quality control, customer first”, and has won unanimous praise from domestic and foreign customers.

Company Profile and Corporate Culture

Company Profile:

ZhiShang Chemical is owned by ZhiShang Group is a professional new-type chemicals enterprise combined into research and development, production and sales .

The company’s competitive product is pharmaceutical raw materials and intermediates (especially carbohydrate derivatives Series), In recent years, the company has made a major breakthrough in food and feed additives, plant extraction, industrial chemicals industry .

The company insists on the spirit of “sincere management, strict quality control, customer as god” , get consistent high praise from customers at home and abroad.

Corporate Culture:

Help China Chemicals to benefit the happiness of human life
Become the most trusted chemical supplier in the world
Striver – oriented, enrich employees, customer first, deep service, seek development
Be prepared for danger in times of peace, forge ahead actively, unity and cooperation, and be brave to fight

About Us

The production base is located in Zhangqiu chemical industry park and Tai’an high-tech chemical industry park. laboratory and workshop in strict accordance with the GMP standard and the product fit national ISO9001 and ISO2000 standards.

“Zhishang” products are exported to Europe, North and South America, the Middle East, Asia Pacific and Africa area, so as to establish a long-term and stable cooperation relationship with customer in the world.

Company Info
  • Business Type: Manufacturer
  • Product Range: Cosmetic raw materials
  • Products/Service:Cosmetic raw materials (flavors and fragrances,antioxidant products, moisturizing and hydrating, whitening agents, cosmetic additives)
  • Total Employees: 51~100
  • Capital (Million US $): 10000000RMB
  • Year Established: 2016
Production Capacity
  • No. of Production Lines : 8
  • No. of QC Staff : 5 -10 People
  • OEM Services Provided : yes
  • Factory Size (Sq.meters) : 3,000-5,000 square meters
  • Certificate: ISO9001 , CE , GMP , API , MSDS
  • Factory Location : Diao Town Industry Park, Zhangqiu City, Jinan City, Shandong Province, China.


Pre-Sales Service

* Prompt reply and 24 hours online, professional team to provide best price and high quality product.

* Sample testing support.

* Every batch of products will be tested to ensureits quality.

*The packing also can be according the customers` requirment.

*Any inquiries will be replied within 24 hours.

*we provide Commerical Invoice, Packing List, Bill of loading, COA , Health certificate and Origin certificate. If your markets have any special requirements, let us know.


After-Sales Service

*The fact of logistics information monitoring.

* Any questions about the product can be consulted at any time.

*Product has any problem can return.


Do you accept sample order?

We will make samples before mass production, and after sample approved, we’ll begin mass production. Doing 100% inspection during production, then do random inspection before packing.



You can get free samples for some products,you only need to pay the shipping cost or arrange a courier to us and take the samples. You can send us your product specifications and requests,we will manufacture the products according to your requests.

What’s your MOQ?

Our MOQ is 1kg. But usually we accept less quantity such as 100g on the condition that sample charge is 100% paid.

Do you supply product report?

Yes. We’ll give you product analysis report before shipping.

  Is there a discount?

Different quantity has different discount.


1. ≤50kg, Express delivery recommended, usually called as DDU service;

2. ≤500kg, Air shipping recommended, usually called as FOB, CFR, or CIF service;

3. >500kg, sea shipping recommended, usually called as FOB, CFR, or CIF service;

4. For high value products, please select air shipping and express delivery for safe.

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