Proteose Peptone CAS 73049-73-7

(1) Raw materials Animal and plant cells with high protein content are superb resources for the manufacturing of Proteose Peptone. Most of the domestically produced peptones are made from bones and fascia left over from the production of canned food and also meat items in canneries as well as meat handling plants, and also fish ribs, low-value small garbage fish and fishmeal from the production of fish fillets in aquatic item processing plants. The continuing to be bean cakes (which can produce plant-based peptone) are used as raw materials when oil mills create oil.
When creating Proteose Peptone, the adipose tissue in the raw material should be eliminated to stop it from affecting the subsequent process as well as item top quality.
( 2) Hydrolysis Put 1000kg of fresh small miscellaneous fish into an acid and also antacid resistant reaction pot with a stirrer, warm and simmer for 1 hr. Let it cool to 45 ° C, add ammonia water to readjust the pH to 8-9, add 125kg of minced icy porcine pancreas, keep the temperature level at 45 ° C, continue hydrolysis for 7 hrs, after that raise the temperature to 50 ° C, continue hydrolysis for 2 hours, and the hydrolysis is primarily finished. Stir frequently during hydrolysis.
When inspecting whether the hydrolysis is total, take a little hydrolyzate, add hydrochloric acid to readjust the pH to 0.5, boil as well as filter, take 5ml of the filtrate, include 20ml of saturated zinc sulfate option, if there is a small amount of floc precipitated, it implies that the hydrolysis has been completed; if there is a huge quantity of floc If there is a form, it indicates that the hydrolysis is not total as well as needs to continue hydrolysis.
( 3) After the ammonia removal, neutralization and hydrolysis are completed, seal the response pot, heat it to 110-115 ° C, open the air launch shutoff, and also release the air to remove the ammonia for 2-2.5 hrs. After the ammonia elimination is finished, gradually include antarctic acetic acid while preserving a mild boiling state, and change the pH of the hydrolyzate to concerning 5.5. Warmth as well as boil for 15 mins, great and also let stand for 12 hours. Siphon the supernatant, filter it with a nylon bag, and gather the filtrate. Include water twice the quantity of the deposit to the filteringed system residue, warm and boil for 15 mins, and filter. The 2nd filtrates were combined.
( 4) Eliminate tyrosine and contaminations. Pump those filtrate into the focus pot. When the vacuum level is not less than 90kPa as well as the temperature is not greater than 80 ° C, when it is focused to a viscous state, put it into a barrel and also send it to below 0 ° C. Location it in the fridge for 2 days to separate out the tyrosine and fat in the concentrate. Eliminate the drifting fat layer from the cold-treated focused solution, add water to thin down to 12 ° Bé, and after that make use of a centrifuge to get rid of tyrosine, and then filter the centrifuged service to information by vacuum cleaner filtering.
( 5) Elimination of fundamental protein Pump the filtrate into a pressure cooker, change the pH to 9.2 with ammonia water, warm to 120 ° C for 10 minutes, cool down to 10 ° C with water, and filter under lowered pressure to eliminate standard healthy protein. The filtrate after elimination of standard healthy proteins have to be cleared up.
( 6) Concentrate and completely dry. Heat the filtrate to bring it to a slight boil up until it is concentrated to 15 ° Bé. The focused service is dried with a call vacuum cleaner stove or a spray clothes dryer. The benefit of using a vacuum cleaner drying out oven for drying out is that the drying out is performed at reduced temperature, which can prevent the product from overheating and is much less contaminated by air. Its disadvantage is that it can not be generated constantly, the labor intensity is high, and the product color is dark. The benefits of spray drying out are: quick drying out speed, constant automated production, reduced labor intensity, and light item shade. Its drawbacks are: large equipment investment and high energy intake. When spray drying, the feed fluid have to be pre-heated before spray drying out to release the air in the feed liquid to make the tissue compact as well as heavy powder, otherwise the quantity of the product will certainly raise and the output will certainly be influenced.
( 7) Product Packaging Vacuum-dried Proteose Peptone is in the form of a block, which is ground by a ball mill prior to packaging; spray-dried peptone remains in powder kind and also can be straight packaged in brown glass or plastic containers.