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Polysorbate 20 CAS 9005-64-5

Molecular Formula: C26H50O10

Formula Weight: 522.6692

ZSpharmac: Polysorbate 20 Supplier

Product Name: Polysorbate 20
CAS No: 9005-64-5
Purity: 99%

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Basic Info

Product Name:Polysorbate 20
Other Names:Tween 20
Place of Origin:Shandong, China
Brand Name:ZSpharmac
Type:Cosmetic Raw Materials
Appearance:Amber Viscous Liquid
EINECS No.:500-018-3
Storage:Store Below +30°C.
Provide:Polysorbate 20 MSDS;
Polysorbate 20 COA

What is Polysorbate 20?

Polysorbate 20 (Tween 20 ), also called polysorbate 20 (polysorbate20 ), is a non-ionic surfactant with solid hydrophilicity and also chemical non-dissociation, which can help liquify medications better It is commonly added in pharmaceutical preparations to boost the solubility of energetic components; on the other hand, Polysorbate 20 can prevent the automatic polymerization of macromolecular protein medications and also enhance the security of drugs. As a brand-new sort of nonionic surfactant, polysorbate 20 has high stability, is not quickly affected by strong electrolytes, acids, and also antacid, can be blended with various other types of surfactants, and also has great compatibility. It has excellent solubility in all sort of solvents. As a result, the consumption of polysorbate 20 is likewise raising day by day. During use, drainage having polysorbate 20 certainly gets in the water body, dirt and various other atmospheres. Non-ionic surfactants are poisonous to fish and also aquatic microorganisms, influence the migration and deterioration of various other existing side-by-side raw material in the setting, and also their adsorption on soil/sediments can limit their application in synergistic remediation of organic air pollution such as dirt. And also with the deepening of pharmacological research study over the last few years, people have actually understood that polysorbate surfactants have particular organic as well as pharmacological activities, which can induce degranulation of cells and also trigger the release of sensitive energetic materials. It is likewise extremely simple to oxidize and break down to generate a range of harmful parts, which are related to damaging responses such as dyspnea, nausea, throwing up, and breakouts that show up scientifically in the drug.

Polysorbate 20 Property

Melting point 

98.9 °C (decomp)

Boiling point 

100 °C


1.11 g/mL at 20 °C

vapor pressure 

<1.4 hPa (20 °C)

refractive index 

n20/D 1.468(lit.)


>230 °F

storage temp. 

Store below +30°C.


100 g/L


viscous liquid

Specific Gravity

1.090~1.130 (20/20℃)




6-8 (50g/l, H2O, 20℃)

PH Range


Water Solubility 

100 g/L



Uses of Polysorbate 20

1. Polysorbate 20 is a non-ionic surfactant. It is made use of as an oil-in-water emulsifier, and can be made use of as a solvent, diffusing representative, stabilizer, lubricating substance as well as antistatic representative. Gas chromatography fixed liquid (maximum use temperature 120 ° C) for separation and also evaluation of unpredictable oils, fatty acid esters, alcohols, ketones and halides.

2. Polysorbate 20 has the function of refolding antigen, which can improve the specific recognition capacity. When doing western blot, obstructing the unbound websites on the membrane with inert protein or non-ionic detergent can lower the non-specific binding of antibodies. The obstructing agent ought to block all unbound websites without displacing the target protein on the membrane, not binding to the target protein website, and also not cross-reacting with antibodies or discovery reagents.

3. Polysorbate 20 is utilized as a cleaning agent, and also the focus ought to not surpass 0.3% (0.05% works best). If the securing result of milk is not good, the focus of Tween can be raised.

The Tween in the WB washing remedy is the same as BSA, which provides security to the antigen-antibody protein. At the same time, since it is a surfactant, it can reduce the non-specific effect of the antibody on the antigen, and also is made use of to elute unbound antibodies and decrease non-specificity. combined. Tween 20 is when we use TBST, the dosage of Tween 20 is 0.5 ml/L, without including SDS.

Polysorbate 20 can be prepared by condensing sorbitol and also its mono-anhydrous, double-anhydrous compounds and laurates with around 20 moles of ethylene oxide per mole of sorbitol and its dehydrated compounds under alkaline problems. It is mostly used in solubilization and also diffusion.

Polysorbate 20 Determination Method

The method for spectrophotometric analysis of nonionic surfactant polysorbate 20 is realized by the following technical scheme:

1) Color reaction: Since the aqueous solution of polysorbate 20 is very light in color, it is necessary to select an appropriate reagent to react with it to form a colored compound before measurement. Iodine, as an electron acceptor, can interact with the oxygen atom in the polysorbate 20 molecule to form a yellow complex. Accurately weigh 1g l2 and 2g Kl, add distilled water to dissolve, and dilute to 100mL. A mixture of Kl-l2 is used as a developer. The prepared chromogen should be stored in the dark and at low temperature.

2) Conditions for color development: the optimal pH range of the solution is 4-8, the amount of color developer is 0.25mL, the color development time is 20min, the optimal absorption wavelength is 486nm, and the reference solution is distilled water.

3) Standard curve: the purpose of making the standard curve is to establish the quantitative relationship between the measurement signal of the method and the concentration of the analyte polysorbate 20. Accurately prepare polysorbate 20 standard solutions with different concentrations. Add 10mL of standard solution of known concentration to the test tube, then add 0.25mL of chromogen, shake well, and let it stand for 20min to react. Use a spectrophotometer at 486nm, use a 1cm cuvette, and use distilled water as a reference to measure the absorbance values of different concentrations of polysorbate 20 and the color developer blank. The difference between the two is the difference between the solution at different concentrations and the reagent blank. absorbance value. The standard curve was obtained by plotting the concentration against the absorbance value.

4) Determination of polysorbate 20 content: Add 10 mL of polysorbate 20 solution of unknown concentration into a test tube, add 0.25 mL of chromogen, shake well, and let it stand for 20 minutes to react. With a spectrophotometer at 486nm, use a 1cm cuvette to record the absorbance value of unknown concentration polysorbate 20, and substitute the absorbance value into the established standard curve equation in step (3) to obtain the content of polysorbate 20 .

The Production Method of Polysorbate 20

Put 1 mol of Span 20 into the reaction kettle, add 50% liquid caustic soda as catalyst under stirring, gradually raise the temperature, dehydrate under reduced pressure, replace the air in the kettle with nitrogen after the water is completely removed, drive away the air and then raise the temperature to 120°C to start 20mol ethylene oxide is introduced, the reaction temperature is maintained at 150-170°C, and the pressure is 0.2-0.3MPa. After passing through the ethylene oxide, cool to 80-90°C, put the feed liquid into the neutralization tank and neutralize it with glacial acetic acid, then decolorize it with hydrogen peroxide, finally cool down and decolorize, and finally cool down and discharge the material, and the packaging is Tween 20.

About Products:
If you need Polysorbate 20 as a cosmetic raw material, please contact our service staff directly, we will provide you with high-quality products at the best price.
As an experienced Polysorbate 20 manufacturer and supplier, ZSpharmac will provide Polysorbate 20 raw materials to customers all over the world for a long time.
Over the years, the company has been adhering to the spirit of “integrity management, strict quality control, customer first”, and has won unanimous praise from domestic and foreign customers.

Company Profile and Corporate Culture

Company Profile:

ZhiShang Chemical is owned by ZhiShang Group is a professional new-type chemicals enterprise combined into research and development, production and sales .

The company’s competitive product is pharmaceutical raw materials and intermediates (especially carbohydrate derivatives Series), In recent years, the company has made a major breakthrough in food and feed additives, plant extraction, industrial chemicals industry .

The company insists on the spirit of “sincere management, strict quality control, customer as god” , get consistent high praise from customers at home and abroad.

Corporate Culture:

Help China Chemicals to benefit the happiness of human life
Become the most trusted chemical supplier in the world
Striver – oriented, enrich employees, customer first, deep service, seek development
Be prepared for danger in times of peace, forge ahead actively, unity and cooperation, and be brave to fight

About Us

The production base is located in Zhangqiu chemical industry park and Tai’an high-tech chemical industry park. laboratory and workshop in strict accordance with the GMP standard and the product fit national ISO9001 and ISO2000 standards.

“Zhishang” products are exported to Europe, North and South America, the Middle East, Asia Pacific and Africa area, so as to establish a long-term and stable cooperation relationship with customer in the world.

Company Info
  • Business Type: Manufacturer
  • Product Range: Cosmetic raw materials
  • Products/Service:Cosmetic raw materials (flavors and fragrances,antioxidant products, moisturizing and hydrating, whitening agents, cosmetic additives)
  • Total Employees: 51~100
  • Capital (Million US $): 10000000RMB
  • Year Established: 2016
Production Capacity
  • No. of Production Lines : 8
  • No. of QC Staff : 5 -10 People
  • OEM Services Provided : yes
  • Factory Size (Sq.meters) : 3,000-5,000 square meters
  • Certificate: ISO9001 , CE , GMP , API , MSDS
  • Factory Location : Diao Town Industry Park, Zhangqiu City, Jinan City, Shandong Province, China.


Pre-Sales Service

* Prompt reply and 24 hours online, professional team to provide best price and high quality product.

* Sample testing support.

* Every batch of products will be tested to ensureits quality.

*The packing also can be according the customers` requirment.

*Any inquiries will be replied within 24 hours.

*we provide Commerical Invoice, Packing List, Bill of loading, COA , Health certificate and Origin certificate. If your markets have any special requirements, let us know.


After-Sales Service

*The fact of logistics information monitoring.

* Any questions about the product can be consulted at any time.

*Product has any problem can return.


Do you accept sample order?

We will make samples before mass production, and after sample approved, we’ll begin mass production. Doing 100% inspection during production, then do random inspection before packing.



You can get free samples for some products,you only need to pay the shipping cost or arrange a courier to us and take the samples. You can send us your product specifications and requests,we will manufacture the products according to your requests.

What’s your MOQ?

Our MOQ is 1kg. But usually we accept less quantity such as 100g on the condition that sample charge is 100% paid.

Do you supply product report?

Yes. We’ll give you product analysis report before shipping.

  Is there a discount?

Different quantity has different discount.


1. ≤50kg, Express delivery recommended, usually called as DDU service;

2. ≤500kg, Air shipping recommended, usually called as FOB, CFR, or CIF service;

3. >500kg, sea shipping recommended, usually called as FOB, CFR, or CIF service;

4. For high value products, please select air shipping and express delivery for safe.

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