1. Removal of phlorizin Phloridin is insoluble in water however conveniently soluble in natural solvents. Most of the conventional methods are the application of natural solvent extraction, as well as currently the majority of them are ultrasonic-assisted extraction, microwave-assisted extraction and also various other techniques. Utilizing apple branches as resources, phlorizin was extracted twice with 10 times the quantity of water as solvent, complied with by open macroporous resin chromatography and also gradient elution with ethanol. The crudely extracted phlorizin was incorporated as well as made use of for medium and also low pressure preparative chromatography, ODS was made use of as a filler, as well as methanol and also water (3:7) were used for elution, and also the concentrated items were incorporated for recrystallization to acquire a pure item.
Contact HPLC chromatography, the result reveals that sample pureness gets to greater than 98%. Xu Kai et al. contrasted different techniques such as ultrasonic method as well as microwave approach, as well as lastly figured out the optimum extraction procedure using L16 (43) orthogonal experiment testing: utilize red Fuji apple as basic material, 40% ethanol service as extraction solvent, and the product The liquid proportion was 1:30, as well as the reflux extraction was performed for 1 hour, as well as the best removal result was gotten. In the study results of Feng Jinyu et al., it was located that when ultrasonic-assisted extraction of phlorizin was executed, the extraction temperature was 45 ° C, the extraction solvent was 70% ethanol solution, and also 30 times the quantity of solvent was included for 35 mins to draw out the most effective effect. Due to the fact that the content of removed phlorizin is typically reduced, additional contamination elimination and also separation and filtration processes are needed to acquire phlorizin with higher pureness.
2. Separation and also filtration of phlorizin Some literature mentions that the reason the purity of phloridzin is really low is that there are a lot more flavonoids and flavonoids in the plant of Polyacae, and also its structure is complicated, as well as the by-products are relatively low. Several, Yang Dajian and others have identified 3 main dihydrochalcones in pleasant tea, which are phlorizin, trilobatin, as well as 3-hydroxyphloridin in turn. Presently, in the existing literary works, it can be ended that the splitting up techniques of phlorizin include macroporous material adsorption method, chitosan flocculation method, high performance centrifugal partition chromatography (HPCPC), polyamide column separation, removal approach and more. Li Shenghua and others showed that D101 macroporous material has the very best separation and purification result on phlorizin, and also its purity can get to 95.6%.
In the study of Sun L et al., X-5 and polyamide material were used to divide epicatechin, hyperoside as well as phlorizin successively. The splitting up pureness can get to more than 95%. This technique is not just practical as well as reduced in expense, but also minimizes the danger of migration. kind. Wang Huiying used the chitosan flocculation method to get a mass fraction of phlorizin of 21.7%, which is close to the confirmation examination outcomes. Chitosan does not need heating as well as conserves sources and also is better for massive commercial use. For the first time, Li Rongtao and also others used HPPCC chromatography to divide and also purify the ethanol extract of apple branches, and gotten phlorizin with a purity of 91.2%. High-efficiency centrifugal partition chromatography has the advantages of high performance, rapidity, high healing price and also high loading ability. Normally speaking, various methods can divide and also purify phlorizin with higher pureness, and the approach can be picked according to one’s own scenario to attain the experimental purpose.