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Zhishang Chemical

Peroxidase CAS 9003-99-0

Molecular Formula: NULL

Formula Weight: 0

ZSpharmac: Peroxidase Supplement

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Product Name: Peroxidase
CAS No: 9003-99-0
Purity: 99%

Basic Info

Product Name:Peroxidase
Other Names:Peroxidase From Horseradish
CAS:9003-99-0
Place of Origin:Shandong, China
Brand Name:ZSpharmac
Type:Food Additives
Appearance:Red-Brown Powder
EINECS No.:232-668-6
Storage:-20°C
Provide:Peroxidase MSDS;
Peroxidase COA

What is Peroxidase?

Horseradish Peroxidase (Horseradish Peroxidase, HRP, E.C. 1.11.1.7) is a glycoprotein composed of enzyme healthy protein as well as iron porphyrin. The enzyme is composed of multiple isozymes, with a molecular weight of 40kDa as well as an isoelectric factor. is pH3.0 9.0. The optimum absorption ranges of the prosthetic group and enzyme protein of HRP are at 403nm and 275nm respectively, so people normally use the RZ value (that is, the ratio of OD403nm/OD275nm) to represent the purity of the enzyme. The bigger the RZ value, the greater the pureness.
There are various isoenzymes, some with strong substrate uniqueness as well as some without specificity. Cofactors can additionally be different, such as glutathione peroxidase consisting of selenium; NAD + peroxidase including craze; cytochrome c peroxidase including heme and so on.Red cell also consist of peroxidase, a really small amount of blood, in the presence of peroxides, can oxidize benzidine or guaiac material into colored materials, which is the concept of examining feces as well as pee “occult blood”.

Peroxidase Properties:

torage temp. -20°C
solubility H2O: soluble
form powder
color red-brown
Water Solubility Soluble in water and phosphate buffer.

 

Peroxidase Uses

  1. Peroxidase exists in mammalian mammary, salivary, as well as lacrimal glands and also their respective secretions, ie, milk, saliva, as well as tears. It is among the usual components in bovine and human milk and exists in all mammalian milks that have actually been evaluated to day. Peroxidase is the very first secreted peroxidase discovered as well as plays a crucial function in securing the mammary glands of breast feeding females and the intestinal tract of babies from pathogenic bacteria and becomes part of the body’s defense system. The peroxidases in the mammary, salivary as well as lacrimal glands are chemically and immunologically similar.
  2. Peroxidase is made use of in biochemical research study as well as is a significant enzyme utilized in the enzyme labeling method. It is utilized in medication to establish the web content of sugar and also galactose in organic fluids, and is used as an analysis enzyme.

Peroxidase Detection Method

A rapid quantitative detection method for peroxidase in pork, characterized in that the detection method comprises the following steps:
(1) prepare a series of standard enzyme solutions with horseradish peroxidase as the standard enzyme;
(2) Using carbamide peroxide as the reaction substrate, using 3,3′,5,5′-tetramethylbenzidine as the color developing agent, and using the sodium dihydrogen phosphate buffer as the buffer, test step (1) respectively The standard enzyme solution prepared in the test reaction temperature is 20-30 ℃, the reaction pH value is 4.0-5.0, and the reaction time is 30-90s. After the reaction is completed, add dilute sulfuric acid solution to terminate the reaction to obtain the reaction product solution;
(3) place each reaction product solution in an ultraviolet spectrophotometer, measure its OD value under the absorption wavelength of 450nm respectively, obtain the standard curve of horseradish peroxidase concentration-OD value;
(4) Test the OD value of the reaction product solution after the peroxidase reaction in the pork to be tested, and calculate the peroxidase concentration in the pork according to the standard curve.

Preparation Method of Peroxidase

CN201510367541.2 provides a lactoperoxidase extraction method that can be used in industrial production with high recovery rate and purification multiple and can be used in food industry. The object of the present invention is achieved through the following technical solutions: take cow whey as raw material, take ammonium sulfate salting-out method as extraction means, determine saturation, salting-out time, optimal salting-out condition in ammonium sulfate salting-out method The process conditions of rotational speed and system pH were compared, and the effects of ammonium sulfate fractional precipitation and direct single-use 65% ammonium sulfate saturation on the recovery rate of LP enzyme activity and purification times were compared. Ultrafiltration technology is used for further purification, and finally dialysis desalination and freeze-drying are used to obtain the finished product, namely raw whey → ammonium sulfate salting out method for fractional precipitation and extraction of lactoperoxidase → precipitation and dissolution → solution separation by ultrafiltration → collection of retentate solution → dialysis Desalting → freeze drying → crude lactoperoxidase product. Concretely, a kind of method that adopts ultrafiltration to assist ammonium sulfate salting out to extract lactoperoxidase of the present invention, comprises the following steps:
(1) Fractional extraction of lactoperoxidase by ammonium sulfate salting out method:
After removing the fat and casein in the fresh milk, whey is obtained. At room temperature, add 25-85% ammonium sulfate to the whey, centrifuge, remove the precipitate, and keep stirring on a mixer to make ammonium sulfate. Fully dissolve, adjust the pH of the system to 5.5-8.5, leave it for 2-10h to completely precipitate the lactoperoxidase, and centrifuge at 5000-10000r/min at 4°C for 20min until the precipitation is the lactoperoxidase product;
(2) Ultrafiltration:
The precipitate obtained in step (1) is reconstituted with an appropriate amount of deionized water and subjected to ultrafiltration separation with an ultrafiltration membrane to remove impurity proteins and collect the intercepted solution;
(3) Desalination treatment:
The retentate solution collected in step (2) is loaded into the dialysis bag for desalination;
(4) Drying:
The crude lactoperoxidase product is obtained by freeze-drying the desalted solution in step (3).
The crude product of lactoperoxidase extracted by the present invention is light yellow powder, and the enzyme activity recovery rate and purification multiple of lactoperoxidase reach 79.25-85.21% and 4.08-4.58 times respectively. The lactoperoxidase with higher recovery rate can be directly obtained through the above-mentioned extraction process, the natural antibacterial medium lactoperoxidase product can be directly obtained, and the product can also be directly added to food as a natural preservative.
Through the above extraction method, lactoperoxidase with high recovery rate can be directly obtained, and natural antibacterial medium lactoperoxidase products can be directly prepared, and the products can also be directly added to daily necessities such as food and toothpaste as natural preservatives. middle.
2. Horseradish Peroxidase (Horseradish Peroxidase, HRP, E.C.1.11.1.7) is a glycoprotein composed of enzyme protein and iron porphyrin. The enzyme consists of multiple isozymes with a molecular weight of 40kDa. The isoelectric point is pH3.0‑9.0. The maximum absorption spectra of the prosthetic group and enzyme protein of HRP are at 403nm and 275nm, respectively, so people usually use the RZ value (ie the ratio of OD403nm/OD275nm) to represent the purity of the enzyme. The larger the RZ value, the higher the purity.
HRP is one of the most important medical diagnostic enzymes (J. Chromat. B 2004, 803:237‑241). This enzyme is not only used in many biochemical detection items, but also widely used in immunological kits. In addition, HRP can also be used for decolorization of industrial wastewater (Chem. Technol. Biotechnol. 2009, 84: 126–132), removal of peroxides and treatment of phenolic wastewater (Biotechnol. Bioprocess Eng. 2006, 11: 462–465), etc.
In nature, horseradish is the plant with the highest HRP content, so it has become the most attractive raw material for the production of HRP. At present, the main methods for separating and extracting HRP from horseradish are: two-phase extraction technology (Appl. Biochem. Biotechnol. 1995, 53: 147-154), reverse micelle extraction technology (Enzyme Microb. Technol. 1996, 18: 332-339), electrophoresis (J. Biol. Chem. 1966, 241: 2166-2172) and chromatography (J. Membr. Sci. 2003, 211: 101-111), etc. However, these technologies all have certain defects. Among them, the products obtained by aqueous two-phase extraction technology and reverse micelle extraction technology are of low purity, and are prone to inactivation and denaturation of HRP; electrophoresis technology and chromatography technology are expensive, and Difficult to scale up industrially.

About Us

The production base is located in Zhangqiu chemical industry park and Tai’an high-tech chemical industry park. laboratory and workshop in strict accordance with the GMP standard and the product fit national ISO9001 and ISO2000 standards.

“Zhishang” products are exported to Europe, North and South America, the Middle East, Asia Pacific and Africa area, so as to establish a long-term and stable cooperation relationship with customer in the world.

Company Info
  • Business Type: Manufacturer
  • Product Range: Additive , Chemical Auxiliary & Catalyst , Organic Chemicals
  • Products/Service: Organic Intermediate,Inorganic Chemistry, APIs, Dyestuffs And Pigments, Fragrance And Spices, Food Additives
  • Total Employees: 51~100
  • Capital (Million US $): 10000000RMB
  • Year Established: 2016
Production Capacity
  • No. of Production Lines : 8
  • No. of QC Staff : 5 -10 People
  • OEM Services Provided : yes
  • Factory Size (Sq.meters) : 3,000-5,000 square meters
  • Certificate: ISO9001 , CE , GMP , API , MSDS
  • Factory Location : Diao Town Industry Park, Zhangqiu City, Jinan City, Shandong Province, China.

Service

Pre-Sales Service

* Prompt reply and 24 hours online, professional team to provide best price and high quality product.

* Sample testing support.

* Every batch of products will be tested to ensureits quality.

*The packing also can be according the customers` requirment.

*Any inquiries will be replied within 24 hours.

*we provide Commerical Invoice, Packing List, Bill of loading, COA , Health certificate and Origin certificate. If your markets have any special requirements, let us know.

 

After-Sales Service

*The fact of logistics information monitoring.

* Any questions about the product can be consulted at any time.

*Product has any problem can return.

FAQ

Do you accept sample order?

We will make samples before mass production, and after sample approved, we’ll begin mass production. Doing 100% inspection during production, then do random inspection before packing.

 

HOW TO CONFIRM THE PRODUCT QUALITY BEFORE PLACING ORDERS?

You can get free samples for some products,you only need to pay the shipping cost or arrange a courier to us and take the samples. You can send us your product specifications and requests,we will manufacture the products according to your requests.

What’s your MOQ?

Our MOQ is 1kg. But usually we accept less quantity such as 100g on the condition that sample charge is 100% paid.

Do you supply product report?

Yes. We’ll give you product analysis report before shipping.

  Is there a discount?

Different quantity has different discount.

Shipping

1. ≤50kg, Express delivery recommended, usually called as DDU service;

2. ≤500kg, Air shipping recommended, usually called as FOB, CFR, or CIF service;

3. >500kg, sea shipping recommended, usually called as FOB, CFR, or CIF service;

4. For high value products, please select air shipping and express delivery for safe.

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