Peptonas CAS 73049-73-7

Eliminate fat and also various other sundries from fresh beef bones, reduced 100kg right into small pieces, add 180kg of water, chef in a spherical rotary stove, the vapor pressure is 3kg/cm 2, and the cooking time is 2.5 h. After cooling down and standing still, take the center layer Bone adhesive solution; readjust the temperature level of the middle layer bone adhesive option to 50 ° C, change the pH value to 6.5, include 2kg of compound protease consisting of 5 parts of trypsin, 8 parts of papain as well as 3 parts of neutral protease for enzymolysis for 5 hrs; after completion of the enzymolysis, Include hydrochloric acid to the middle bone adhesive option, readjust the pH worth to 5.2, and heat the slurry to 95 ° C to suspend the enzyme. The enzyme inactivation time is 12 mins, and then cool and precipitate for 1 hour. The middle clear fluid is taken as the first-grade Peptonas liquid.

The main peptone service goes through a plate as well as frame filter to get rid of big bits of pollutants to obtain a secondary peptone remedy; the second peptone option travels through an ultrafiltration maker with an accuracy of 0.15 μm to remove great contaminations such as huge molecular weight bone healthy protein to obtain a Peptonas tertiary remedy; Peptonas quality 3 remedy is concentrated by a three-effect evaporator to get peptone quality 4 remedy; the peptone quality 4 remedy is co-opted by a high-pressure homogenizer to get Peptonas grade 5 option.

The Peptonas fifth-grade fluid was input right into the spray drying tower for drying out, and the air inlet temperature of the drying tower was set at 150 ° C., and the air outlet temperature level was 80 ° C. to get 14.26 kg of peptone powder. Compute according to every 100 kilograms of fresh beef bones containing 15kg of healthy protein, the academic yield of healthy protein in the peptone powder is 95%; after the actual content of healthy protein in the peptone powder is measured, it is established that the content of protein in the Peptonas powder is 98.2%, and also the typical molecular weight is 3150Da, the peptone powder of this plan is industrial type Peptonas.

Get rid of fat and also other sundries from the fresh beef bone, cut 50kg right into little pieces, add 75kg of water, cook in a round rotary stove, the vapor stress is 2kg/cm 2, as well as the cooking time is 5h. After cooling down as well as stalling, take the middle bone adhesive service; change the temperature level of the bone glue solution in the center layer to 52 ° C, change the pH worth to 6.9, include 1.5 kg of compound protease made up of 12 parts of trypsin, 6 parts of papain as well as 5 neutral proteases for enzymolysis for 3 hrs; after completion of the enzymolysis, Add hydrochloric acid to the middle bone adhesive service, change the pH value to 5.0, and also heat the middle layer bone adhesive option to 100 ° C to suspend the enzyme. The enzyme inactivation time is 15 minutes, and after that awesome and also speed up for 1 hour. The center clear fluid is taken as the first-grade Peptonas remedy.

The key peptone option is removed by a plate and framework filter to get rid of big bits of impurities to get a second peptone solution; the additional peptone solution is infiltrated an ultrafiltration machine with a precision of 0.15 μm to eliminate great pollutants such as large molecular weight bone healthy protein to get a third-grade peptone remedy; The third-grade peptone solution is focused by a three-effect evaporator to acquire the fourth-grade peptone remedy; the fourth-grade peptone option is co-opted by a high-pressure homogenizer to acquire the fifth-grade peptone option.

The Peptonas fifth-grade fluid was input into the spray drying tower for drying, and the air inlet temperature of the drying out tower was evaluated 180 ° C., and the air outlet temperature was 60 ° C. to get 14.68 kg of peptone powder. Compute according to every 100 kgs of fresh beef bones having 15kg of protein, the academic yield of protein in the peptone powder is 95%; after the real material of healthy protein in the peptone powder is measured, it is established that the material of protein in the Peptonas powder is 96.3%, as well as the average molecular weight is 2800Da, the Peptonas powder in this scheme is reagent type Peptonas.