Paeonol CAS 552-41-0

( 1) Take about 5 mg of this item, add 1 ml of ethanol to liquify, include 0.5 ml of agarazine sulfonic acid test remedy, shake well, add 1 decrease of sodium bicarbonate examination option, as well as progressively become orange-red.
( 2) Take this product and also liquify it in ethanol to make a service including concerning 5μg per 1ml. According to the spectrophotometry (Appendix IV A of Chinese Pharmacopoeia 2000 Version), the maximum absorption is at the wavelengths of 314nm and also 274nm, and also the There is marginal absorption at the wavelengths of 295 nm and also 244 nm.
( 3) In the chromatogram taped under the web content resolution item, the retention time of the major height of the test substance should be consistent with the retention time of the recommendation material optimal of paeonol.

The clarity as well as color of ethanol service take 1.0 g of this product, include 10ml of ethanol to liquify, the remedy must be clear as well as anemic; if it is turbid, compare it without any. It has to not be thicker; if the shade is developed, it needs to not be darker than the basic colorimetric service of brown-red No. 2 or orange-red No. 2 (Chinese Pharmacopoeia 2000 Version, Part II, Appendix IX A, Technique 1).
For chloride, take 0.5 g of this item, add 30ml of water, warmth to dissolve, cool down, include 10ml of weaken nitric acid, filter, take the filtrate, check according to the law (Chinese Pharmacopoeia 2000 Version Part II Appendix VIII A), and basic sodium chloride solution 5.0 ml Compared to the prepared control option, it needs to not be a lot more concentrated (0.01%).
Take 1g of this item for sulfate, include 25ml of water, shake for 10 mins, filter, take the filtrate, and check according to the regulation (Appendix VIII B, Component II of the Chinese Pharmacopoeia 2000 Version). More focused (0.03%).
Pertinent materials were examined according to high performance fluid chromatography (Appendix V D of Chinese Pharmacopoeia 2000 Edition). Use octadecylsilane adhered silica gel as filler; methanol/FONT >( 50:50) as mobile stage; discovery wavelength is 275nm. The number of academic plates need to not be less than 2000 according to the paeonol height. Take this product, add methanol to make a remedy having 2mg per 1ml, as the test service; precisely measure an ideal amount, add methanol to make a remedy including 0.02 mg per 1ml, as a control service. Take 20 μl of the control remedy and infuse it right into the liquid chromatograph to change the detection sensitivity to ensure that the top elevation of the main part chromatographic height is 10% to 30% of the full scale; To twice the retention time of the major element optimal. If there are impurity tops in the chromatogram of the examination solution, the amount of the areas of the contamination peaks will not be above the main top location of the control service.

loss on drying out
Take this item, put it in a phosphorus pentoxide desiccator and dry it under minimized stress to constant weight, as well as the weight management ought to not exceed 1.0% (Chinese Pharmacopoeia 2000 Version, Part II, Appendix VIII L).

Residue on ignition
Not more than 0.1% (Chinese Pharmacopoeia 2000 edition of the 2nd appendix VII N).

heavy metal
Take the residue left under the product of deposit on ignition, and check it according to the legislation (Appendix VIII H, Second Approach of Chinese Pharmacopoeia, 2000 Edition), and also the heavy metal material must not go beyond 10 components per million.

According to gas chromatography (Chinese Pharmacopoeia 2000 edition of the two appendix VI E) resolution.

Chromatographic conditions and also system viability test Polyethylene glycol (PEG) -20 M was used as the stationary stage, and the layer focus was 10%; the column temperature was 200 ° C. The number of theoretical plates should not be less than 2000 calculated according to the paeonol peak, as well as the resolution in between the paeonol peak and also the internal conventional compound peak must meet the needs.

Decision of modification variable Take a proper quantity of biphenyl, add petroleum ether (60 ～ 90 ℃) to make an option containing 0.5 mg per 1ml, as the internal basic remedy. In addition, regarding 50 mg of paeonol reference compound was taken, accurately evaluated, and also placed in a stoppered conelike flask; 50 ml of interior common service was added exactly, trembled, and 2 μl was infused right into the gas chromatograph, and the adjustment element was determined according to the peak location.

Assay

Take 50 mg of this product, properly evaluate it, and also place it in a stoppered conical flask; properly include 50 ml of the internal typical remedy, tremble well, soak up 2 μl, infuse it right into a gas chromatograph, procedure as well as determine.