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Paeoniflorin Usp CAS 23180-57-6

Molecular Formula: C23H28O11

Formula Weight: 480.47

ZSpharmac: Paeoniflorin Usp Supplement

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Product Name: Paeoniflorin Usp
CAS No: 23180-57-6
Purity: 99%

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Basic Info

Product Name:Paeoniflorin Usp
Other Names:Paeoniflorin Extract
CAS:23180-57-6
Place of Origin:Shandong, China
Brand Name:ZSpharmac
Type:Plant Extracts
Appearance:White Powder
EINECS No.:245-476-2
Storage:2-8°C
Provide:Paeoniflorin Usp MSDS;
Paeoniflorin Usp COA

What is Paeoniflorin Usp?

Paeoniflorin Usp can withstand oxidative anxiety injury of tissue cells, prevent the activation of astrocytes, as well as boost the protection of nerves. It can be utilized to deal with Alzheimer’s disease, Parkinson’s disease, epilepsy and also various other mind diseases. On top of that, Paeoniflorin Usp can also battle autoimmune diseases such as tumor, rheumatoid arthritis and also ankylosing spondylitis. Animal experiments additionally confirmed that paeoniflorin can substantially minimize blood sugar level degrees as well as has an important safety impact on cardiopulmonary cells.

Paeoniflorin Usp Properties:

Melting point approximate 124℃ (dec.)
Boiling point 498.51°C (rough estimate)
density 1.3044 (rough estimate)
refractive index 1.5376 (estimate)
storage temp. 2-8°C
solubility Methanol (Slightly, Sonicated), Pyridine (Slightly)
pka11.52±0.70(Predicted)
form powder
color white

 

Paeoniflorin Usp Uses

  1. A glycoside separated from Paeoniflorin root, Paeoniflorin Usp is a glycoside separated from Paeoniflorin origin. Paeoniflorin Usp has actually been made use of as an anticonvulsant and cannabis chemistry book glycemic result. The substance turns around guanethidine-induced hypotension by triggering adenosine A1R (central adenosine A1-Rs) in the mind.
  2. Paeoniflorin Usp Chinese natural active ingredients originate from the origin of Paeonia suffruticosa. Chinese medication on its antispasmodic residential or commercial properties. Anti-myocardial anemia, anti-coagulation and also restraint of platelet gathering; antispasmodic result; analgesic and sedative results; anti-inflammatory as well as anti-ulcer results;
    Clinical application: for the therapy of coronary heart disease; for aged conditions, to enhance physical and also immune feature, anti-inflammatory as well as relieving cough, expectorant and also alleviating bronchial asthma, etc, especially in the treatment of senior persistent breathing diseases can be made use of as an auxiliary medicine.

Paeoniflorin Usp Preparation

1. Investigation of static adsorption performance of macroporous resin
Weigh 1kg of Baishao decoction pieces, extract twice with 8L 70% ethanol for 2 hours each time, filter, combine the filtrates, and concentrate under reduced pressure until there is no alcohol smell.
Weigh 1.00g of the dry weight of each type of resin, put it in ten 50ml conical flasks with stoppers, add 10ml of Paeonia lactiflora extract (0.5g crude drug/ml), and shake it in a constant temperature shaking box at 35°C for 6 hours. The vibration speed was 180 prm, the adsorbed Paeoniflorin extract was vacuum filtered, the filtrate was appropriately diluted, and the contents of Paeoniflorin Usp and Paeoniflorin were determined by HPLC with reference to the 2010 version of the Pharmacopoeia method. The adsorbed resin was washed 3 times with water, dried in vacuum, added with 10 ml of 95% ethanol, and shaken for 6 hours at 35°C in a constant temperature shaking box for desorption at a vibration speed of 180 prm. The desorbed solution was appropriately diluted and the content of Paeoniflorin Usp and Paeoniflorin was determined by HPLC. The adsorbed resin was washed 3 times with water, dried in vacuum, added with 10 ml of 95% ethanol, and shaken for 6 hours at 35°C in a constant temperature shaking box for desorption at a vibration speed of 180 prm. The desorbed solution was appropriately diluted and the content of Paeoniflorin Usp and Paeoniflorin was determined by HPLC.

2. Preparation of Paeonia lactiflora Extract
Weigh 40kg of Paeonia lactiflora medicinal materials, soak them in 70% ethanol overnight, extract by reflux for 2 times, 2h each time, filter, combine the filtrates, rotary evaporate until there is no alcohol smell, adjust the liquid medicine so that each 1ml liquid medicine is equivalent to 0.5g crude drug, spare. Weigh 120kg (wet weight) LX38 resin, put it into a stainless steel column (300×2300cm), the column volume is 60L, load the sample, after adsorption for 1h, first remove impurities with 300L of water and 300L of 10% ethanol, and then use 360L of 50% ethanol. Paeoniflorin and paeoniflorin were eluted, and finally the resin was regenerated with 95% ethanol, the flow rate was 150-200L/h, and the 50% ethanol fraction was concentrated and evaporated to dryness to obtain 2.52kg, wherein the purity of Paeoniflorin Usp was 24%, and that of paeoniflorin was 16%. %.

3. Preparation of monomer components by medium pressure silica gel column
Take 8 g of the Paeonia suffruticosa extract obtained in Example 2, dissolve it in methanol, add 8 g of 200-300 mesh silica gel and mix the sample, use a medium pressure column system for separation, use silica gel H to pack a column (150 × 35 mm i.d.), and elute with ethyl acetate 1000ml, flow rate 50mL/min, then eluted with ethyl acetate:methanol=95:5, detected by ultraviolet 230nm, collected Paeoniflorin Usp fraction and paeoniflorin fraction in turn, concentrated under reduced pressure, and dried to obtain 2.8g of paeoniflorin, Paeoniflorin 1.2g, the purity is 95.4% and 92.2% respectively.

Research Progress on The Neuroprotective Mechanism of Paeoniflorin Usp

1.1 Reduce the level of oxygen free radicals The activity of superoxide dismutase (SOD) in tissues is often used as the main indicator of the ability to scavenge oxygen free radicals. The level of malonaldehyde (MDA) reflects the generation level of oxygen free radicals in the body. A large amount of MDA can damage the structure and function of biofilms in brain tissue, and cause protein denaturation and inactivation. Glutathione (GSH) is a small-molecule antioxidant that plays important roles in the storage and transport of reduced sulfur, the synthesis of proteins and nucleic acids, the regulation of enzymatic activity, the maintenance of tissue antioxidant properties, and the sensitivity to redox. plays an important role in the regulation of signal transduction. Catalase (CAT) is a class of oxidase widely present in animals, plants and microorganisms, and its function is to catalyze the decomposition of intracellular hydrogen peroxide and prevent oxidation. In recent years, studies have found that in the corticosterone-induced PC12 cell injury model, the expression of SOD was decreased and the expression of MDA was increased. In vivo experiments showed that in Aβ1-42-treated rat brain cholinergic nerve injury model, long-term treatment with PF enhanced its cognitive performance in the Morris water maze test, restored SOD and CAT levels, decreased MDA levels, and improved spatial learning and memory impairment.

1.2 Regulation of nitric oxide (NO) and inducible nitric oxide synthase (iNOS) content NO has various effects in the central nervous system, which affects brain function and the integration of afferent signals. In the central nervous system, excess NO is neurotoxic. High levels of NO are associated with the pathological processes of aging-related diseases. NOS produces NO in vivo and catalyzes the production of a large number of neurotoxic NO molecules, leading to neuronal cell hypoxia, edema, and apoptosis. A single β-amyloid (amyloid β-protein, Aβ) can induce the increase of NOS activity and NO level in rat hippocampus, and PF has a preventive effect on its induced neurotoxicity. NO may damage DNA and irreversibly modify proteins, such as tyrosine nitration or thiol oxidation, which are pathogenic mechanisms of several common neurodegenerative diseases. On the other hand, NO, which is derived from peroxynitrite, may cause irreversible damage to mitochondria, resulting in reduced ATP formation, induction of mitochondrial permeability transition pore opening, release of cytochrome c and activation of Caspase. In a rat acute spinal cord injury model, PF (20 mg/kg) treatment downregulated iNOS expression and increased neuronal survival in an acute spinal cord injury model.

1.3 Activation of adenosine A1 receptors Adenosine is an endogenous neuroprotective factor in the central nervous system, which can relieve cell excitotoxicity and Ca2+ overload by activating presynaptic A1 receptors and inhibiting the release of excitatory amino acids. thereby protecting cells. Zhong et al. found in the oxygen and glucose deprivation experiments of cerebral cortical neurons that the activation of Akt and ERK1/2 by low concentrations of PF (10 nmol/L-1 μmol/L) depends on the transfer of adenosine A1 receptors to activate EGFR (epidermal growth factor). receptor), and then exert its neuroprotective effect against oxygen sugar deficiency. The potential for A1R agonists to treat cerebral ischemia is controversial because A1R agonists can cause several serious cardiovascular side effects. In rat models of transient and permanent focal ischemia (15 min and 6 h of ischemia), the effects of subcutaneous injection of PF (2.5 mg/kg and 5 mg/kg) on infarct volume and nerve damage were observed, and it was found that PF In a dose-dependent manner, the cortex, subcortical nucleus and total infarct size were reduced in the transient cerebral ischemia model, and the neuroprotective effect of the transient occlusion model was better than that of the permanent occlusion model. The neuroprotective effects of PF could be abolished by pretreatment with DPCPX, a selective A1 antagonist, 0.25 mg/kg, suggesting that PF may have neuroprotective effects against cerebral ischemia by activating A1R with less cardiovascular side effects Or not, PF may have potential value as an anti-stroke drug.

Mitochondria are important organelles that generate energy in cells, and the functional changes of mitochondria play an important role in apoptosis. Mitochondrial damage mainly includes changes in Bcl-2 family proteins, endoplasmic reticulum stress and lysosome-induced changes in mitochondrial membrane permeability. The mitochondrial membrane permeability transport pore opens and releases a large number of small molecular chemicals, such as cytochrome c, etc. . Studies have shown that PF can increase the mitochondrial membrane potential level in the mitochondrial dysfunction model of SH-SY5Y cells induced by Aβ25-35, while reducing the ratio of Bax/Bcl-2, so the anti-apoptotic effect of PF may be mediated by mitochondrial pathway. realized. The results of Wang et al. found that PF could significantly attenuate streptozotocin (STZ)-induced cognitive deficits in an in vivo animal model of mitochondrial dysfunction, manifested as significantly increased cytochrome c oxidase activity and ATP synthesis, and was associated with STZ. Mitochondrial membrane potential (MMP) was restored in the hippocampus and cortex compared to groups.

3.1 Effects on MAPK signaling pathway The mitogen-activated protein kinase (MAPK) signaling pathway is an important signal transduction system in the body, mediating various physiological and pathological processes such as cell growth, development, differentiation and apoptosis. The main families are ERK, JNK, p38. In the nervous system, the increased activity of ERK mediates the proliferation and differentiation of most neuronal cells. The activated ERK has anti-apoptotic activity. The activation of JNK and p38 is mainly reflected in astrocytes and microglia. Stress and apoptosis are necessary, and activation has a pro-apoptotic effect, thereby affecting the expression of the lower cascade signaling factors Bcl-2, Bax, Caspase-9, and Caspase-3, mediating apoptosis and inflammatory responses.

Studies have shown that PF intervention can protect against TNF-α-induced apoptosis and neuronal loss, and this protective effect of PF may be achieved by inhibiting MAPK/NF-κB-mediated ischemic injury-induced peripheral and brain inflammatory responses of. Zhou et al. found that PF (20, 40, 60 mg/kg) could significantly reduce the expression of p-p38 and p-JNK protein levels in a neuropathic pain rat model, and at the same time reduce the levels of inflammatory factors IL-1 and TNF-α. This effect may be achieved by inhibiting the expression of upstream signal ASK1 and then inhibiting p38 and JNK. Zhong et al. found that PF (30 mg/kg and 60 mg/kg) could increase the expression of p-ERK protein level in a rat model of chronic unpredictable stress disorder, indicating that PF could play a neuroprotective role by regulating ERK signaling pathway.

3.2 Influence on PI3K/Akt signaling pathway PI3K/Akt signaling pathway mediates cell reproduction, differentiation, apoptosis and other reactions, and it has many downstream effectors, among which Akt is the central link of this signal transduction. Akt activation acts on the downstream Bcl-2 family, Caspase family, NF-κB family, etc. The activation of PI3K/Akt signaling pathway can increase the expression of anti-apoptotic protein Bcl-2, and reduce the expression of pro-apoptotic proteins Caspase and Bax. Studies have shown that PF may upregulate Bcl-2 and downregulate the protein expression levels of Caspase-9, Caspase-3 and Bax by activating the PI3K/Akt pathway, thereby protecting neurons from apoptosis. Pretreatment with PF (50-400 mg/L) can increase the protein levels of p-PI3K and p-Akt-1 in neural progenitor cells with H2O2-induced injury, and this effect of PF can be abolished by the PI3K inhibitor LY294002, indicating that PF is mediated by PF. The neuroprotective effect of H2O2 injury depends on the activation of PI3K/Akt-1 pathway.

3.3 Activation of Nrf2/ARE signaling pathway Oxidative stress is an important factor in the initiation and development of many neurodegenerative diseases, and Nrf2/ARE signaling pathway is a key pathway in cellular oxidative stress response. When oxidative stress occurs, Nrf2 dissociates from Keap1, translocates into the nucleus, and binds to antioxidant response elements (AREs) to promote the gene expression of detoxification enzymes and antioxidant enzymes, playing an important role in cellular defense protection.

Nrf2 plays a crucial role in maintaining normal physiological processes in the brain. Nrf2 knockout in mice results in proteasome dysfunction, neuronal apoptosis, age-related forebrain atrophy, and neurobehavioral deficits. PF (10, 50, 100 μmol/L) can promote the expression of Nrf2 protein in the brain tissue of rats with cerebral ischemia-reperfusion injury, and also promote the transfer of Nrf2 from the cytoplasm to the nucleus in PC12 cells in a dose-dependent manner. Knockdown of Nrf2 by siRNA attenuated the protective effect of PF in PC12 cells, and found that its antioxidant and anti-inflammatory activities were weakened, suggesting that PF may regulate the expression of genes related to antioxidant response elements by promoting the expression and activation of Nrf2, thereby reducing cerebral ischemia and reactivation. Exhibits antioxidant and anti-inflammatory effects after perfusion injury.

Calcium ion is an essential ion for various physiological activities of the body, which can maintain the biopotential on both sides of the cell membrane and maintain normal nerve conduction function. The large influx of calcium ions can lead to an increase in the concentration of Ca2+ in neurons, which in turn disrupts the balance of intracellular and extracellular ions, causing neuronal damage. In a model of N-methyl-D-aspartate-induced excitotoxicity in primary hippocampal neurons, massive influx of Ca2+ leads to neuronal cell damage, PF (100 μmol/L and 200 μmol/L) intervention It can improve cell viability and inhibit intracellular Ca2+ concentration.

The results of Wang et al. suggest that PF can inhibit the intracellular Ca2+ overload and the expression of calmodulin kinase II (CaMKII) in the glutamate-induced PC12 cell injury model, and the addition of CaMKII inhibitor KN93 improves cell injury, indicating that PF mediates The neuroprotective effect is mainly achieved by preventing Ca2+ influx and inhibiting the overexpression of CaMKII.

Autophagy is a common and important life phenomenon in eukaryotic cells. When cells are stimulated by internal factors or external factors, autophagy can remove damaged intracellular abnormal substances and use degradation products to provide energy and rebuild cells structure, maintaining cellular homeostasis and cellular life activities.

In Parkinson’s disease, pathological activation of the acid-sensitive ion channel ASIC leads to degeneration of dopaminergic neurons. Gu et al found that PF can down-regulate the expression of ASIC1a in 6-hydroxydopamine-induced Parkinson’s disease animal model, reduce the level of α-synuclein (α-SYN), a special marker of Parkinson’s disease, reduce dopamine and its metabolites, and improve autonomic phagocytosis, delaying degeneration and loss of dopaminergic neurons. At the same time, it was also proved in cell experiments that PF can enhance the autophagic degradation of α-SYN. Ma et al. showed that PF could exert neuroprotective effects by reducing the number of autophagosomes in SH-SY5Y cells induced by okadaic acid.

PF has anti-inflammatory activity and can fight against neuroinflammation to exert a neuroprotective effect. For example, in neurodegenerative diseases, Aβ oligomers are enriched in the brain, which can accelerate the development of neurodegenerative diseases by stimulating the excessive activation of microglia to produce persistent neuroinflammatory responses.

Some studies have found that PF pretreatment can reduce the production of TNF-α, IL-1β, and IL-6 in AD animal models induced by Aβ1-42, and inhibit the phosphorylation of NF-κB, thereby inhibiting the excessive activation of microglia and inhibiting neuroinflammation. reaction. In the in vitro experiments of neuroinflammation, studies have shown that PF can significantly block LPS-induced hippocampal cell death, while inhibiting the levels of NO, TNF-α and IL-1β, and this blocking effect is also by reducing microglial activation. thereby reducing the release of inflammatory factors.

Company Profile and Corporate Culture

Company Profile:

ZhiShang Chemical is owned by ZhiShang Group is a professional new-type chemicals enterprise combined into research and development, production and sales .

The company’s competitive product is pharmaceutical raw materials and intermediates (especially carbohydrate derivatives Series), In recent years, the company has made a major breakthrough in food and feed additives, plant extraction, industrial chemicals industry .

The company insists on the spirit of “sincere management, strict quality control, customer as god” , get consistent high praise from customers at home and abroad.

Corporate Culture:

OUR MISSION
Help China Chemicals to benefit the happiness of human life
OUR VISSION
Become the most trusted chemical supplier in the world
OUR BUSINESS PHILOSOPHY
Striver – oriented, enrich employees, customer first, deep service, seek development
OUR VALUES
Be prepared for danger in times of peace, forge ahead actively, unity and cooperation, and be brave to fight

About Us

The production base is located in Zhangqiu chemical industry park and Tai’an high-tech chemical industry park. laboratory and workshop in strict accordance with the GMP standard and the product fit national ISO9001 and ISO2000 standards.

“Zhishang” products are exported to Europe, North and South America, the Middle East, Asia Pacific and Africa area, so as to establish a long-term and stable cooperation relationship with customer in the world.

Company Info
  • Business Type: Manufacturer
  • Product Range: Additive , Chemical Auxiliary & Catalyst , Organic Chemicals
  • Products/Service: Organic Intermediate,Inorganic Chemistry, APIs, Dyestuffs And Pigments, Fragrance And Spices, Food Additives
  • Total Employees: 51~100
  • Capital (Million US $): 10000000RMB
  • Year Established: 2016
Production Capacity
  • No. of Production Lines : 8
  • No. of QC Staff : 5 -10 People
  • OEM Services Provided : yes
  • Factory Size (Sq.meters) : 3,000-5,000 square meters
  • Certificate: ISO9001 , CE , GMP , API , MSDS
  • Factory Location : Diao Town Industry Park, Zhangqiu City, Jinan City, Shandong Province, China.

Service

Pre-Sales Service

* Prompt reply and 24 hours online, professional team to provide best price and high quality product.

* Sample testing support.

* Every batch of products will be tested to ensureits quality.

*The packing also can be according the customers` requirment.

*Any inquiries will be replied within 24 hours.

*we provide Commerical Invoice, Packing List, Bill of loading, COA , Health certificate and Origin certificate. If your markets have any special requirements, let us know.

 

After-Sales Service

*The fact of logistics information monitoring.

* Any questions about the product can be consulted at any time.

*Product has any problem can return.

FAQ

Do you accept sample order?

We will make samples before mass production, and after sample approved, we’ll begin mass production. Doing 100% inspection during production, then do random inspection before packing.

 

HOW TO CONFIRM THE PRODUCT QUALITY BEFORE PLACING ORDERS?

You can get free samples for some products,you only need to pay the shipping cost or arrange a courier to us and take the samples. You can send us your product specifications and requests,we will manufacture the products according to your requests.

What’s your MOQ?

Our MOQ is 1kg. But usually we accept less quantity such as 100g on the condition that sample charge is 100% paid.

Do you supply product report?

Yes. We’ll give you product analysis report before shipping.

  Is there a discount?

Different quantity has different discount.

Shipping

1. ≤50kg, Express delivery recommended, usually called as DDU service;

2. ≤500kg, Air shipping recommended, usually called as FOB, CFR, or CIF service;

3. >500kg, sea shipping recommended, usually called as FOB, CFR, or CIF service;

4. For high value products, please select air shipping and express delivery for safe.

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