Report one
An Organic Tea Tree Oil atomized inhalation, 1L of the atomized inhalation includes the following components: tea tree oil 5g, soybean lecithin 5g, sodium deoxycholate 0.65g, ethanol 65g, the balance is water, the atomized inhalation The pH of the agent is 7. Prepare as follows:
First, dissolve 5 g of solubilizer I (soybean lecithin) in 65 g of absolute ethanol to obtain solution A; then dissolve 0.65 g of solubilizer II (sodium deoxycholate) in water, and then add 5 g of tea tree oil until the mixed solution is clear to obtain solution B; finally, add solution B to solution A, stir and dilute to 1L with water.
Report two
A preparation method of tea tree oil microcapsules:
S1. Weigh 30g of urea, 70.9g of formaldehyde and 126g of water in a three-necked flask, adjust the pH of the system to 8 with triethanolamine, heat in an oil bath at 70°C for 1h, and the obtained colorless liquid is urea-formaldehyde resin prepolymer .
S2. Weigh 12g of urea-formaldehyde resin prepolymer, put 0.48g of sodium lauryl sulfate in a beaker adding 20ml of water, stir with a magnetic stirrer for 15min under normal temperature conditions, and the rotating speed is 1000r/min to obtain the first mixture;
S3.1) Dissolve 3g of tea tree oil in 6g of absolute ethanol, and dropwise dropwise into the first mixture, adjust the rotating speed of the magnetic stirrer to 800r/min, and stir for 15min; 2) Then adjust the pH of the system to 5, and continue to stir 15min; 3) Adjust the rotational speed to 300r/min and adjust the pH of the system to 3. After stirring for 15 minutes, continue to adjust the pH of the adjustment system to 2 and stir for 30 minutes; 4) Heat the temperature of the system to 30-35°C and cure for 80 minutes.
S4. Suction filtration with a sand core funnel, put the resulting product into a vacuum drying oven at 40° C., and the white powder obtained after 24 hours of complete drying is tea tree oil microcapsules.
Report three
Weigh 5g of propylene glycol and add 85g of purified water to dissolve, then weigh 5g of sodium carboxymethylcellulose and add to the solution to dissolve, then weigh 5g of Delta emulsifier and add to the above solution to dissolve; finally weigh 11.1g of Organic Tea Tree Oil and add to the wound solution , use emulsification equipment to emulsify, and sterilize the fully emulsified tea tree oil gel package to get an anti-itch antibacterial dressing containing tea tree oil.
Report four
1) Preparation of monolayer tea tree oil nanoliposomes
① Weigh 1g of soybean lecithin, 0.2g of cholesterol and 200mg of tea tree oil, add 50mL of chloroform to dissolve.
② Evaporate in a rotary evaporator until the solvent is evaporated to dryness. The evaporation temperature is 10~30°C, and a smooth film will be formed on the inner wall of the round bottom flask; .
③ Weigh 0.05g of PVP into 50mL acetate buffer, diffuse under ultrasonic conditions, then add the PVP acetate buffer into a round bottom flask for hydration under ultrasonic conditions.
④Put the hydrated mixture in the cell ultrafine pulverizer for 30min at a frequency of 10s with a gap of 5s to further make the particle size of the liposome emulsion more uniform and finer.
⑤ Centrifuge the obtained product at 4000rpm for 15min, and take the upper layer liquid.
⑥ Filter the resulting liquid with a 0.22 μm filter membrane to obtain the filtrate, which is a single-layer tea tree oil nanoliposome.
2) Preparation of bilayer tea tree oil nanoliposomes
① According to the preparation method of the above-mentioned single-layer tea tree oil nano-liposome, prepare the single-layer nano-liposome containing 200mg tea tree oil.
② Disperse the monolayer tea tree oil liposomes in the acetate solution containing 0.2mg/mL chitosan and mix evenly; the volume ratio of the monolayer tea tree oil nanoliposomes to the acetate solution of chitosan is 1:10 .
③ The resulting mixture was pulverized for 30 minutes in a cell ultrafine pulverizer at a frequency of 10 seconds and a gap of 5 seconds.
④ Centrifuge the obtained product at 4000rpm for 15min, and take the upper liquid;
⑤ Filter the resulting liquid with a 0.22 μm filter membrane to obtain the filtrate, which is a double-layer tea tree oil nanoliposome.
3) Preparation of multilayered tea tree oil nanoliposomes
① According to the preparation method of the above-mentioned double-layer Organic Tea Tree Oil nano-liposome, prepare the double-layer nano-liposome containing 200mg tea tree oil.
② Disperse the double-layer tea tree oil nanoliposome in the acetate solution containing 0.4mg/mL gelatin to make it evenly mixed; the volume ratio of the double-layer tea tree oil nanoliposome to the acetate solution of gelatin is 1: 10.
③ Pulverize the obtained mixed solution in the cell ultrafine pulverizer for 30 minutes at a frequency of 10 seconds and a gap of 5 seconds;
④ Centrifuge the obtained product at 4000rpm for 15min, and take the upper liquid;
⑤ Filter the resulting liquid with a 0.22 μm filter membrane to obtain the filtrate, which is multilayered tea tree oil nanoliposomes.
Report five
Weigh the prescribed amount of lecithin, cholesterol, tea tree oil, vitamin E, Tween-80, sodium deoxycholate, etc., add an appropriate amount of absolute ethanol, and sonicate until the suspension is uniform to obtain a lipid suspension. The hydration medium is placed on a constant temperature magnetic stirrer to maintain a specified temperature and stirring speed, and the lipid suspension is slowly dropped into it to obtain a liposome initial suspension with an ethanol concentration of 10%. Place in a water-bath ultrasonic instrument and sonicate until the tea tree oil nanoliposome suspension is translucent, and obtain the Organic Tea Tree Oil nanoliposome suspension.
