A technique for preparing mogroside V utilizing molecular purification, which includes the complying with actions:
1) After squashing the monk fruit, pass it through a 40-60 mesh display to get the monk fruit powder. Weigh 60 g of Luo Han Guo powder into a 1000 mL round-bottomed flask, add 500 mL of ethanol with a quantity concentration of 80% into the round-bottomed flask, put the round-bottomed flask into an ultrasonic cleaner, and also soak for 30 secs under ultrasonic problems at 200 KHz. min;
2) After the ultrasonic soaking is completed, warm the round-bottomed flask with an electrical heating mantle as well as execute reflux extraction for 5 hrs. After the reflux extraction is completed, suction filtrate as well as gather the filtrate. The filtrate was concentrated under reduced stress to eliminate the majority of the solvent to get monk fruit remove (the extract is a viscous fluid paste). The return of monk fruit essence was 30.12%;.
3) The monk fruit essence went through the very first molecular purification under the problems of scrape rate of 140 rad/min, purification temperature level of 40 ° C, purification pressure of 0.1 , as well as feed price of 0.3 mL/min to get light monk fruit essence. Component 1 #, hefty part 2 #, as well as the return of Luo Han Guo hefty component 2 # is 36.51%;.
4) The heavy element 2 # is subjected to the second molecular purification under the conditions of scraper rate of 70 rad/min, purification temperature of 60 ° C, distillation stress of 0.1 Pa, and also feed price of 0.5 mL/min to obtain mogroside-containing The light element 3 # of V (the yield of the light component 3 # of Luo Han Guo remove is 3.63%),.
The light component 3 # having mogroside V was freeze-dried in a freeze clothes dryer for 24 hours to obtain white fine-grained crystals, which is the finished product of mogroside V.
A technique for preparing mogroside V by incorporating subcritical and molecular purification, which includes the complying with actions:.
1) Crush the monk fruit in a mortar as well as pass it with a 40-60 mesh display to obtain monk fruit powder;.
2) Take 200 g of monk fruit powder as well as place it in a subcritical extraction kettle. Add 1200 mL of n-butane as well as dimethyl ether with a quantity proportion of 1:1 right into the subcritical extraction pot as the extraction representative. The removal temperature is 10 ° C and the removal pressure Essence for 3 hrs at 2.0 MPa to acquire Luo Han Guo extract;.
3) Focus the Luo Han Guo extract under minimized pressure to eliminate 60% of the extractant, and afterwards boil down under the conditions of a purification temperature level of 50 ° C, a purification stress of 0.01 , a scraper rate of 50 rad/min, and a feed rate of 0.1 mL/min. Under the problems of molecular distillation, the light part including mogroside V was acquired, and after that freeze-dried by a freeze dryer for 1 day to get white fine-grained crystals, which are the completed products of mogroside V, with a return of 13.65%.
The completed item of mogroside V prepared above underwent high-performance liquid phase discovery, as well as the detection results found that the purity was 96.95%.
