a. Select silymarin seeds with complete grains, laundry and also completely dry them, as well as use a mechanical oil mill to press them utilizing a three-stage pressing approach. 100 catties of silymarin seeds can be ejected to 15 catties of oil.;
B, feed consumption, took into 1.5 lots of silymarin dish after every can of rugged powder, include acetone by the proportion of 1:4, namely include 4 tons of acetone to 1 lots of silymarin dish, accomplish product reflux removal with removal container and also concentration container for 10 hour, material temperature is controlled at 25 levels, and redeems acetone solvent with condenser concurrently;
c, the solvent is filteringed system, concentrated for 2 hours, the temperature is 60 degrees, the vapor stress is 1.5 MPa, as well as the acetone solvent is recovered with a condenser to obtain a paste essence;
d. Placed the essence in a jacketed pot, include 1:5 detoxified water, that is, add 1 kg of extract to 5 kg of water, heat the water to 80 levels, mix for 1 hr, let go the water, repeat 6 times such as this;
e. Placed the silymarin extract right into a vacuum cleaner drying out tank, and also accomplish vacuum cleaner drying at a drying temperature of 120 levels as well as a negative pressure of 0.8 MPa for 4-6 hours continuously to get a dry powder block of silymarin with a water content of concerning 5%. Crush it into 80‑90 mesh;
f. Put the pulverized silymarin into a vacuum tank for a second vacuum, completely dry for 2-4 hours at a temperature of 80 levels, and also an unfavorable pressure of 0.8 MPa to acquire completely dry silymarin powder.
1. Preparation process of compound microbial starter freeze-dried powder
1.1 Activation of the initial pressure
Measure 10ml of 0.9% typical saline into 2 examination tubes, sterilize at 121 ° C for 20 minutes and also great to 28 ° C, put all the freeze-dried bacterial powders in the ampoules of 2 strains right into 0.9% In physical saline, shake to liquify it, and activate it in a 28 ° C incubator for 30 minutes, and also set aside.
1.2 Expanded culture of fermentation bacteria
1.2.1 Preparation of mom starter
Step 200ml of society medium for every of the two stress into 2 500ml Erlenmeyer flasks, sanitize at 121 ° C for 20 minutes and also trendy to 28 ° C, inoculate 10% of the volume of the culture medium with the activated strains in step 1.1, at 28 ° C, The drinking table was cultivated for 1 day under the condition of the shaking table rotating rate of 150 rpm, as the mother starter
1.2.2 Prep work of manufacturing starter.
First prepare skimmed milk emulsion with a mass percentage focus of 10%, and also separate it into 2 500mL Erlenmeyer flasks, each bottle is 200mL, decontaminate at 121 ° C for 20 minutes, cool down to 28 ° C, and inoculate the mom starter at a volume ratio of 2%., 28 ° C, shaker speed 150 rev/min, shaker culture for 48‑52 hrs, discover the variety of feasible germs in the fermentation liquid of both strains, and also the number of practical bacteria in each fermentation liquid is ≥ 109/ml, which is considered as fermentation maturation, if If the number of sensible microorganisms is less than 109/ml, remain to grow till it gets to 109/ml.
1.3 Prep work of powder freeze-dried pressures
Present the fully grown production starter right into a glass ampoule under aseptic conditions, the fluid degree is lower than 1cm, cover the bottle and placed it into a 30 ° C fridge freezer for quick freezing. After freezing, placed the glass ampoule on a tray and put it in the The lyophilizer is used for lyophilization to prepare powder lyophilized strains.
1.4 Preparation of composite microbial starter lyophilized powder
According to the weight of each powder freeze-dried stress, take 3 parts of A microorganisms powder and 2 parts of B bacteria powder, mix well and make composite microbial starter freeze-dried powder.
2. Preparation of Milk Thistle Seed Oil
2.1 Milk thistle seed pretreatment
After the milk thistle seeds are mechanically smashed, the oil is pressed with a screw press to obtain deoiled seed cake powder, as well as water is contributed to adjust the wetness web content of the deoiled seed cake powder to 40% to 45%, according to the weight of the damp deoiled seed cake powder Include sucrose at 4.2% to 4.8%, mix well, sanitize at 121 ° C for 20 minutes and also awesome to 28 ° C to make a fermentation tool for later usage.
2.2 Activation of freeze-dried powder of substance microbial starter
Initially prepare 1 kg of sugar option with a mass percent concentration of 2%, take 5 grams of composite microbial starter freeze-dried powder, as well as completely dissolve it in 1 kg of sugar option at 25 ° C to 28 ° C to make a composite microbial suspension, stall Triggered for half an hour.
2.3 Fermentation
Evenly inoculate 1 kg of the substance microbial suspension turned on in step 2.2 on 10 kg of fermentation medium pretreated in step 2.1, and afte
r that spread the inoculated fermentation tool on a sterile tray, the thickness of the tool is 12cm to 15cm, warm preservation and also fermentation for 60 hours at 25 ° C to
28 ° C. 2.4 drying out
Dry the tool fermented symphonious 2.3 with hot air at 75 ° C to 80 ° C, as well as stir while drying, to make sure that the wetness content of the fermented tool is lower than 5%.
2.5 Extraction Superfinely grate the product processed symphonious 2.4, pass through a 100-mesh filter, take the sieved powder according to the ratio of weight quantity proportion of 1g:15 mL and also include absolute ethanol, at 15 ° C to 20 ° C, and also the vacuum degree is -0.1 MPa After removal for 10 hours, the supernatant was distilled under decreased pressure to recover ethanol after membrane splitting up to acquire a yellow powder, which was dissolved in methanol at a ratio of 1 g: 5 mL. After filtering, the insoluble issue was vacuum-dried to acquire Milk Thistle Seed Oil.
