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Zhishang Chemical

Methylene Blue Trihydrate CAS 7220-79-3

Molecular Formula: C16H24ClN3O3S

Formula Weight: 373.9

ZSpharmac: Methylene Blue Trihydrate Supplier

Product Name: Methylene Blue Trihydrate
CAS No: 7220-79-3
Purity: 99%

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Basic Info

Product Name:Methylene Blue Trihydrate
Other Names:Methylene Blue
Place of Origin:Shandong, China
Brand Name:ZSpharmac
Type:Cosmetic Raw Materials
Appearance:Green Solid
EINECS No.:615-731-6
Storage:No Restrictions.
Provide:Methylene Blue Trihydrate MSDS;
Methylene Blue Trihydrate COA

What is Methylene Blue Trihydrate?

Methylene Blue Trihydrate, also known as alkaline lake blue, methylene blue, methylene blue, molecular formula: C16H18ClN3S•3H2O, is a green, shiny columnar crystal or crystalline powder. Odorless, no change in exposed air, soluble in water and alcohol. Methylene blue is mild in nature and safe to use. It also helps to improve the respiratory function of fish.

Methylene Blue Trihydrate Property

Melting point 190 °C (dec.)(lit.)
density 0.98 g/mL at 25 °C
Fp 14 °C
storage temp. no restrictions.
solubility Soluble in water, chloroform; sparingly soluble in ethanol
form Solid
pka2.6, 11.2(at 25℃)
Colour Index 52015
color Green
Water Solubility 4 g/100 mL


Application of Methylene Blue Trihydrate

Methylene Blue Trihydrate is widely used in chemical indicators, dyes, biological stains and drugs. Especially in the dyeing industry, it is used in the manufacture of ink, etc., and the amount is relatively large. In the following fields, methylene blue is also widely used.
1.Industrial field
Methylene Blue Trihydrate is a dye compound. Industrial-grade methylene blue is often used for dyeing cotton, silk, paper, etc., and can also be used for coloring bamboo and wood and making inks, lakes, etc. In addition, it is also often used as a bacterial stain, indicator, etc.
2.Pharmaceutical field
Methylene Blue Trihydrate has been used in the medical field for a long time. Due to the redox properties of methylene blue, it can be used to relieve cyanide, nitrite, aniline, acetanilide poisoning, or methemoglobinemia caused by drugs such as sulfonamides. In recent years, scholars have also conducted research on the application of methylene blue and its metabolites in various bacterial and viral infections, cancer and central nervous system diseases such as depression, schizophrenia, Alzheimer’s disease and other diseases.
3. Aquaculture field
Methylene Blue Trihydrate can be used as a disinfectant in aquatic product farming. The principle is that the ionic compound formed by Methylene Blue Trihydrate in aqueous solution can compete with the microbial enzyme system for hydrogen ions, inactivate the enzyme, and cause the microorganism to lose its viability; It also has a certain curative effect in the treatment of fish diseases such as small melon worm disease, oblique tube worm disease, red mouth disease, saprolegniasis and Litopenaeus vannamei larval viscera, and can also be used as an antifungal drug to reduce the risk of fish transportation. death rate.
4. areas of skin care
Studies have shown that Methylene Blue Trihydrate also has some potential in skin care. Methylene Blue Trihydrate can scavenge free radicals, stimulate cell proliferation in young and old dermal fibroblasts, and thus have certain effects on improving skin vitality, promoting skin elasticity and collagen synthesis, and protecting the skin matrix through the degradation of matrix metalloproteinases. effect. Therefore, methylene blue can be a promising agent in anti-aging cosmetics.

Preparation of Methylene Blue Trihydrate

Consider 2mol (418g) of p-aminodimethylaniline hydrochloride, placed it right into a reaction kettle with a thermometer, add 6L of water to liquify, include 0.41 mol (40g) of focused sulfuric acid dropwise under mixing to make the service acidic, and also add Zinc chloride option, aluminum sulfate remedy, salt thiosulfate remedy, and also 300mL sodium dichromate service were included, and also the reaction service was heated up to 40 ° C within 10 minutes under stirring, and also the hydrochloric acid solution of N,N-dimethylaniline Put it into the reactor, then instantly add the remaining 600mL salt dichromate option, as well as heat it to 70 ° C within 10 minutes; when the temperature level reaches 70 ° C, placed 2.87 mol (250g) of manganese dioxide right into the reactor, warmth it to 85 ° C, as well as maintain The temperature was reacted for 30 minutes, the temperature was naturally reduced to 50 ° C, as well as 7.14 mol (700g) of focused sulfuric acid was gradually included under stirring; Wash with 600mL liquid sodium chloride solution; move the filter cake to a 20L beaker, add 10L water, stir as well as warm to boiling on the electrical heating mantle, suction filter while it is hot, add 1.84 mol (250g) zinc chloride and also 25.64 mol (1500g) Sodium chloride, stir to dissolve, after that place the remedy at 4 ° C for 24h, suction filter, as well as completely dry at 50 ° C to get 460g of Methylene Blue Trihydrate zinc chloride salt; liquify the obtained zinc chloride salt in 10L of water, add sodium carbonate 0.52 mol (55.2 g), cool to space temperature, filter with suction, clean the filter cake with a small amount of water, include 17.09 mol (1000g) of sodium chloride, stir and liquify, place it at 4 ° C for 12 hrs, filter with suction, and filter the cake at 40-45 After drying out at ℃ for 12 hrs, 320 g of the unrefined product of Methylene Blue Trihydrate was acquired (loss on drying out was about 15%, and also the return was 42.5% based on anhydrous matter). Put the crude methylene blue into a conical flask, include 1280mL of 0.1 mol/L hydrochloric acid (1:4), heat as well as dissolve in a water bathroom at 60 ° C, location formation at space temperature, filter with suction, as well as recrystallize the filter cake when in hydrochloric acid of the same volume and concentration. The exact same quantity of water was recrystallized when, and the filter cake was dried at 40-45 ° C for 12 hrs to acquire 205g of Methylene Blue Trihydrate, with a loss on drying out of 14.6% and also a return of 64.3% based upon anhydrous matter. The complete yield is 27.4% based on the raw material p-aminodimethylaniline hydrochloride.

Main Uses of Methylene Blue Trihydrate

1. New fish quarantine
Methylene Blue Trihydrate can be used in new fish bags or in quarantine tanks for quarantine of new fish before entering the tank. Because of its mild medicinal properties, it is especially suitable for the quarantine of fry and small fish. In addition, it is also commonly used in the postpartum care of broodstock.

2. Control Saprolegnia
When used with salt, it can prevent saprolegniasis and the resulting rot.

3. Prevention and treatment of white spot disease and other external diseases
Spray the Methylene Blue Trihydrate solution in the whole tank, although the effect of the medicine is slow (it usually takes 7 days for a course of treatment, during which half of the water is changed and the medicine is added to maintain the concentration), but the damage to the fish is relatively small. (Recommended by Ito Fish Farm, Japanese players use this method more.)

4. Detoxify nitrite
Because the filter system of the fish tank is too weak or the nitrification system collapses due to other reasons, the nitrite content in the fish tank exceeds the standard and causes fish poisoning. You can use methylene blue medicine bath to treat methaemoglobinemia and rescue sub- Nitrate poisoning phenomenon.

Methylene Blue Trihydrate has a staining effect, the water in the fish tank will turn blue, and it will last for a long time, and it is easy to combine with the filter material, so it is best not to use this medicine in the original tank.

About Products:
If you need Methylene Blue Trihydrate as a cosmetic raw material, please contact our service staff directly, and we will provide you with high-quality products at the best price.
As an experienced manufacturer and supplier of Methylene Blue Trihydrate, ZSpharmac will provide Methylene Blue Trihydrate raw materials to customers all over the world for a long time.
Over the years, the company has been adhering to the spirit of “integrity management, strict quality control, customer first”, and has won unanimous praise from domestic and foreign customers.

Company Profile and Corporate Culture

Company Profile:

ZhiShang Chemical is owned by ZhiShang Group is a professional new-type chemicals enterprise combined into research and development, production and sales .

The company’s competitive product is pharmaceutical raw materials and intermediates (especially carbohydrate derivatives Series), In recent years, the company has made a major breakthrough in food and feed additives, plant extraction, industrial chemicals industry .

The company insists on the spirit of “sincere management, strict quality control, customer as god” , get consistent high praise from customers at home and abroad.

Corporate Culture:

Help China Chemicals to benefit the happiness of human life
Become the most trusted chemical supplier in the world
Striver – oriented, enrich employees, customer first, deep service, seek development
Be prepared for danger in times of peace, forge ahead actively, unity and cooperation, and be brave to fight

About Us

The production base is located in Zhangqiu chemical industry park and Tai’an high-tech chemical industry park. laboratory and workshop in strict accordance with the GMP standard and the product fit national ISO9001 and ISO2000 standards.

“Zhishang” products are exported to Europe, North and South America, the Middle East, Asia Pacific and Africa area, so as to establish a long-term and stable cooperation relationship with customer in the world.

Company Info
  • Business Type: Manufacturer
  • Product Range: Cosmetic raw materials
  • Products/Service:Cosmetic raw materials (flavors and fragrances,antioxidant products, moisturizing and hydrating, whitening agents, cosmetic additives)
  • Total Employees: 51~100
  • Capital (Million US $): 10000000RMB
  • Year Established: 2016
Production Capacity
  • No. of Production Lines : 8
  • No. of QC Staff : 5 -10 People
  • OEM Services Provided : yes
  • Factory Size (Sq.meters) : 3,000-5,000 square meters
  • Certificate: ISO9001 , CE , GMP , API , MSDS
  • Factory Location : Diao Town Industry Park, Zhangqiu City, Jinan City, Shandong Province, China.


Pre-Sales Service

* Prompt reply and 24 hours online, professional team to provide best price and high quality product.

* Sample testing support.

* Every batch of products will be tested to ensureits quality.

*The packing also can be according the customers` requirment.

*Any inquiries will be replied within 24 hours.

*we provide Commerical Invoice, Packing List, Bill of loading, COA , Health certificate and Origin certificate. If your markets have any special requirements, let us know.


After-Sales Service

*The fact of logistics information monitoring.

* Any questions about the product can be consulted at any time.

*Product has any problem can return.


Do you accept sample order?

We will make samples before mass production, and after sample approved, we’ll begin mass production. Doing 100% inspection during production, then do random inspection before packing.



You can get free samples for some products,you only need to pay the shipping cost or arrange a courier to us and take the samples. You can send us your product specifications and requests,we will manufacture the products according to your requests.

What’s your MOQ?

Our MOQ is 1kg. But usually we accept less quantity such as 100g on the condition that sample charge is 100% paid.

Do you supply product report?

Yes. We’ll give you product analysis report before shipping.

  Is there a discount?

Different quantity has different discount.


1. ≤50kg, Express delivery recommended, usually called as DDU service;

2. ≤500kg, Air shipping recommended, usually called as FOB, CFR, or CIF service;

3. >500kg, sea shipping recommended, usually called as FOB, CFR, or CIF service;

4. For high value products, please select air shipping and express delivery for safe.

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