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Methylene Blue Trihydrate CAS 7220-79-3

Molecular Formula: C16H20ClN3OS

Formula Weight: 337.87

ZSpharmac: Methylene Blue Trihydrate Supplier

Product Name: Methylene Blue Trihydrate
CAS No: 7220-79-3
Purity: 99%

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Basic Info

Product Name:Methylene Blue Trihydrate
Other Names:Methylene Blue
Place of Origin:Shandong, China
Brand Name:ZSpharmac
Type:Cosmetic Raw Materials
Appearance:Green Solid
EINECS No.:615-731-6
Storage:No Restrictions.

Methylene Blue Trihydrate

Methylene Blue Trihydrate COA

What is Methylene Blue Trihydrate?

Methylene blue, also known as basic lake blue, methylene blue, methylene blue, molecular formula: C16H18ClN3S•3H2O, is a green, shiny columnar crystal or crystalline powder. Odorless, no change in exposed air, soluble in water and alcohol. Methylene blue is mild in nature and safe to use. It also helps to improve the respiratory function of fish.

Methylene Blue Trihydrate Property

Melting point 190 °C (dec.)(lit.)
density 0.98 g/mL at 25 °C
Fp 14 °C
storage temp. no restrictions.
solubility Soluble in water, chloroform; sparingly soluble in ethanol
form Solid
pka2.6, 11.2(at 25℃)
Colour Index 52015
color Green
Water Solubility 4 g/100 mL


Methylene Blue Trihydrate Uses

Methylene Blue Trihydrate is generally taken advantage of in chemical indicators, dyes, natural discolorations and medications. Specifically in the coloring market, it is made use of in the manufacture of ink, and more, and also the quantity is reasonably big. In the complying with locations, methylene blue is likewise generally used.
1Industrial location
Methylene Blue Trihydrate is a color substance. Industrial-grade methylene blue is generally made use of for coloring cotton, silk, paper, and so forth, as well as can likewise be utilized for tinting bamboo as well as also wood and making inks, lakes, etc. Additionally, it is additionally often used as a microbial discolor, indicator, and so on

2Pharmaceutical field
Methylene Blue Trihydrate has been used in the professional area for a long period of time. As a result of the redox structures of methylene blue, it can be used to soothe cyanide, nitrite, aniline, acetanilide poisoning, or methemoglobinemia caused by drugs such as sulfonamides. In the last few years, scholars have actually in addition executed research on the application of methylene blue as well as likewise its metabolites in various microbial as well as viral infections, cancer and additionally main nervous system disease such as anxiety, schizophrenia, Alzheimer’s problem as well as other disease.

3 Tank farming area
Methylene Blue Trihydrate can be utilized as a disinfectant in water item farming. The idea is that the ionic compound developed by methylene blue in aqueous treatment can take on the microbial enzyme system for hydrogen ions, put on hold the enzyme, as well as likewise develop the germ to shed its stability; It similarly has a details curative impact on the treatment of fish illness such as small melon worm illness, oblique tube worm illness, red mouth condition, saprolegniasis as well as Litopenaeus vannamei larval viscera, along with can furthermore be made use of as an antifungal drug to decrease the death of fish throughout transportation Rate.

4 places of skin therapy
Studies have revealed that Methylene Blue Trihydrate likewise has some feasible in skin treatment. Methylene blue can feed on cost-free radicals as well as promote cell spreading in young and also old dermal fibroblasts, hence increasing skin vitality, advertising and marketing skin flexibility as well as collagen synthesis, and securing the skin matrix through the devastation of matrix metalloproteinases. As a result, methylene blue can be a promising agent in anti-aging cosmetics.

Preparation of Methylene Blue Trihydrate

Consider 2mol (418g) of p-aminodimethylaniline hydrochloride, positioned it right into a response kettle with a thermostat, include 6L of water to dissolve, add 0.41 mol (40g) of focused sulfuric acid dropwise under mixing to make the service acidic, and also consist of Zinc chloride service, light weight light weight aluminum sulfate solution, sodium thiosulfate solution, and also 300mL salt dichromate alternative were included, as well as additionally the response treatment was heated to 40 ° C within 10 mins under mixing, and also the hydrochloric acid alternative of N,N-dimethylaniline Put it right into the reactor, afterwards right away include the remaining 600mL salt dichromate choice, as well as warm it to 70 ° C within 10 mins; when the temperature gets to 70 ° C, put 2.87 mol (250g) of manganese dioxide right into the activator, cozy it to 85 ° C, as well as also maintain The temperature level was reacted for thirty minutes, the temperature level was typically minimized to 50 ° C, as well as also 7.14 mol (700g) of concentrated sulfuric acid was progressively included under stirring; Wash with 600mL liquid sodium chloride solution; move the filter cake to a 20L beaker, include 10L water, mix and additionally warmth to steaming on the electric home heating mantle, suction filter while it is cozy, add 1.84 mol (250g) zinc chloride along with 25.64 mol (1500g) Sodium chloride, mix to liquify, after that placed the option at 4 ° C for 24h, filter with suction, and entirely dry at 50 ° C to obtain 460g of methylene blue zinc chloride salt; liquify the acquired zinc chloride salt in 10L of water, consist of 0.52 mol of sodium carbonate (55.2 g), stylish to location temperature degree, filter with suction, clean the filter cake with a small amount of water, include 17.09 mol (1000g) of sodium chloride, mix and liquify, position it at 4 ° C for 12 hours, filter with suction, along with entirely dry the filter cake at 40-45 ° C After 12 humans resources, 320 g of raw methylene blue was obtained (loss on drying had to do with 15%, as well as the yield was 42.5% based on anhydrous problem). Location the unrefined Methylene Blue Trihydrate right into a conelike flask, include 0.1 mol/L hydrochloric acid 1280mL (1:4), warmth to liquify in a water shower room at 60 ° C, location condensation at space temperature, filter with suction, recrystallize the filter cake when in hydrochloric acid of the similar quantity and also focus, The specific same quantity of water was recrystallized when, along with the filter cake was dried at 40-45 ° C for 12 hrs to obtain 205 g of methylene blue, with a loss on drying of 14.6% and also a return of 64.3% based upon anhydrous concern. The overall return is 27.4% based upon the raw product p-aminodimethylaniline hydrochloride.

Method for Detecting Sulfide in Water Quality With Methylene Blue Trihydrate

1. Establish a standard curve
Take six 100ml colorimetric tubes with stoppers, add 20ml of sodium hydroxide absorption solution to each, measure 0.00ml, 0.50ml, 1.00ml, 2.00ml, 4.00ml, 7.00ml of sulfide standard solution into each colorimetric tube, Add water to about 60ml, slowly add 10ml of N,N-dimethyl-p-phenylenediamine solution along the wall of the colorimetric tube, seal it immediately and turn it over slowly once, pull out the plug and open a small opening, add 1ml ferric ammonium sulfate solution, immediately seal it and fully Shake well, prepare the standard sequence with concentration of 0.00ug/L, 5.0ug/L, 10.0ug/L, 20.0ug/L, 40.0ug/L, 70.0ug/L, after standing for 10min, rinse with deionized water or ultra Dilute to the mark with pure water and shake well. Use a 10mm cuvette and use deionized water or ultrapure water as a reference to measure the absorbance at a wavelength of 665nm. Take the content of sulfide (ug) as the abscissa, and take the absorbance value after deducting the zero concentration point as the ordinate to draw a standard curve for measuring high-concentration samples.

Measure 0.00ml, 0.20ml, 0.50ml, 1.00ml, 1.50ml, 2.00ml of sulfide standard solution respectively, and prepare the concentration as 0.00ug/L, 2.0ug/L, 5.0ug/L, 10.0 ug/L, 15.0ug/L, 20.0ug/L standard sequence, using 30mm cuvette for color comparison, draw a standard curve for the determination of low concentration samples.

2. Water sample analysis
Operate the water sample after acidification and distillation according to the above standard curve establishment steps, then measure its absorbance, and compare the parameters of the blank sample measured in the same step to obtain the accurate content of sulfide in the water sample.

About Products:
If you need Methylene Blue Trihydrate as a cosmetic raw material, please contact our service staff directly, and we will provide you with high-quality products at the best price.
As an experienced manufacturer and supplier of Methylene Blue Trihydrate, ZSpharmac will provide Methylene Blue Trihydrate raw materials to customers all over the world for a long time.
Over the years, the company has been adhering to the spirit of “integrity management, strict quality control, customer first”, and has won unanimous praise from domestic and foreign customers.

Company Profile and Corporate Culture

Company Profile:

ZhiShang Chemical is owned by ZhiShang Group is a professional new-type chemicals enterprise combined into research and development, production and sales .

The company’s competitive product is pharmaceutical raw materials and intermediates (especially carbohydrate derivatives Series), In recent years, the company has made a major breakthrough in food and feed additives, plant extraction, industrial chemicals industry .

The company insists on the spirit of “sincere management, strict quality control, customer as god” , get consistent high praise from customers at home and abroad.

Corporate Culture:

Help China Chemicals to benefit the happiness of human life
Become the most trusted chemical supplier in the world
Striver – oriented, enrich employees, customer first, deep service, seek development
Be prepared for danger in times of peace, forge ahead actively, unity and cooperation, and be brave to fight

About Us

The production base is located in Zhangqiu chemical industry park and Tai’an high-tech chemical industry park. laboratory and workshop in strict accordance with the GMP standard and the product fit national ISO9001 and ISO2000 standards.

“Zhishang” products are exported to Europe, North and South America, the Middle East, Asia Pacific and Africa area, so as to establish a long-term and stable cooperation relationship with customer in the world.

Company Info
  • Business Type: Manufacturer
  • Product Range: Cosmetic raw materials
  • Products/Service:Cosmetic raw materials (flavors and fragrances,antioxidant products, moisturizing and hydrating, whitening agents, cosmetic additives)
  • Total Employees: 51~100
  • Capital (Million US $): 10000000RMB
  • Year Established: 2016
Production Capacity
  • No. of Production Lines : 8
  • No. of QC Staff : 5 -10 People
  • OEM Services Provided : yes
  • Factory Size (Sq.meters) : 3,000-5,000 square meters
  • Certificate: ISO9001 , CE , GMP , API , MSDS
  • Factory Location : Diao Town Industry Park, Zhangqiu City, Jinan City, Shandong Province, China.


Pre-Sales Service

* Prompt reply and 24 hours online, professional team to provide best price and high quality product.

* Sample testing support.

* Every batch of products will be tested to ensureits quality.

*The packing also can be according the customers` requirment.

*Any inquiries will be replied within 24 hours.

*we provide Commerical Invoice, Packing List, Bill of loading, COA , Health certificate and Origin certificate. If your markets have any special requirements, let us know.


After-Sales Service

*The fact of logistics information monitoring.

* Any questions about the product can be consulted at any time.

*Product has any problem can return.


Do you accept sample order?

We will make samples before mass production, and after sample approved, we’ll begin mass production. Doing 100% inspection during production, then do random inspection before packing.



You can get free samples for some products,you only need to pay the shipping cost or arrange a courier to us and take the samples. You can send us your product specifications and requests,we will manufacture the products according to your requests.

What’s your MOQ?

Our MOQ is 1kg. But usually we accept less quantity such as 100g on the condition that sample charge is 100% paid.

Do you supply product report?

Yes. We’ll give you product analysis report before shipping.

  Is there a discount?

Different quantity has different discount.


1. ≤50kg, Express delivery recommended, usually called as DDU service;

2. ≤500kg, Air shipping recommended, usually called as FOB, CFR, or CIF service;

3. >500kg, sea shipping recommended, usually called as FOB, CFR, or CIF service;

4. For high value products, please select air shipping and express delivery for safe.

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