Method 1. Removal approach
Saturating, alkali refining, acidification Put about 2-3 tons of faucet water in the algae washing storage tank, put in 120kg of algae, and also after the algae broaden, carefully clean the Mannitol Pulver on the algae right into the water, and also the washed seaweed is made use of for removing sodium alginate. Clean the second set of seaweed with the washing fluid, and also clean about four batches like this. Include 300g/L (30%) sodium hydroxide option to the above cleaning remedy, Ph10-11, and also allow it stand for 8 hours until the fucoidan service, starch as well as other organic thick materials are fully coagulated and also sped up. Siphon the supernatant, acidify it with sulfuric acid (1:1), readjust it to pH 6-7, better get rid of the jelly, and obtain a neutral supernatant.
Seaweed or kelp [faucet water] → lotion [NaOH] → [pH10-11, 8h] supernatant [H2SO4] → [pH6-7] neutral clear liquid focused, alcohol clean the above neutral clear liquid
Warmth it with direct fire or heavy steam up until it steams as well as evaporates, the temperature is 110-115 ° C, a huge amount of sodium chloride speeds up, and also the salt and also adhesive dirt are continuously removed till it becomes a concentrated solution, take a little sample and pour it on the ground, as well as it should strengthen after a little air conditioning The feeding material contains greater than 30% Mannitol Pulver and also regarding 10% moisture. Trendy the concentrated remedy to 60-70 ° C, include 95% ethanol (2:1) while it is hot, keep mixing, and also progressively trendy to room temperature, after that centrifuge to completely dry to remove periodontal, and also acquire off-white loosened matter.
Neutral clear liquid [110-115 ° C] → concentrate [ethanol] → [60-70 ° C] loosened issue extraction Consider the loosened issue, put it into a removal pot furnished with a reflux condenser, add 94% of 8 times the quantity Ethanol, stirred, gradually heated up, steamed as well as refluxed for 30 minutes to discharge, cooled in running water for 8 hrs, left for a day and night, centrifuged and also dried out to get white loosened crude mannitol, containing 70% -80% mannitol. The same procedure as above, ethanol recrystallization once, to obtain Mannitol Pulver for commercial usage, the content is more than 90%, and the Cl- web content is <0.5%.
Loose matter [94% ethanol] → [reflux for 30 minutes, cooling for 8 hours] refine unrefined mannitol, include industrial mannitol and also pure water to liquify, after that add 1/10 -1/ 8 medicinal triggered carbon, keep stirring, maintain warm at 80 ℃ for half hours, filter while warm, clean the turned on carbon twice with a little water, integrate the cleaned filtrate, as well as concentrate up until the mannitol gets to 70%, if there is turbidity, re-filter. Great to room temperature level under stirring, take shape, filter with suction, clean the crystals, as well as filter with suction to obtain crystalline mannitol.
Unrefined mannitol [pure water, triggered carbon] → [80 ° C, 30min] focus the filtrate → crystallize mannitol as well as dry the above crystallized mannitol, after the Cl- is qualified (Cl-< 0.007%), completely dry it with vapor at 105-110 ° C, transformed frequently, and also obtained in 4 hours, it is the completed item of medical mannitol. Web content 98% -102%, melting point 166-168 ° C, [α] 20D +23 °- +24 °. Crystalline mannitol [105-110 ° C, 4h] → Preparation of ended up medicinal mannitol injection The ended up mannitol is dissolved in water for injection according to the dose to create an option, and also mannitol injection is acquired after sanitation. Mannitol completed item.
[Water for Shot] → Mannitol Injection.
Method 2, straight fermentation method.