In view of this, the object of the present invention is to provide a process for extracting and purifying mangiferin using a biologically active enzyme method, which not only improves the yield of the product, but also improves the purity of the product, while greatly reducing the production cost. The process is simple and feasible, and meets the conditions of large-scale production.
In order to solve the above technical problems, the technical solution provided by the invention is: a preparation method of mangiferin, the preparation method comprising the following steps:
(1), get raw material mango leaf, through drying, pulverizing, soak 0. Ihr～1. Ohr with the enzyme solution of 6～10 times raw material weight, at pH4.0～7.0 and 40 ℃～55 ° C lower water bath enzymolysis 1hr～4hr, and fully stir;
(2), filter, get medicinal dregs and carry out degreasing process 2～3 times with the sherwood oil of 6～10 times of weight respectively;
(3), step (2) the dregs after the degreasing treatment are extracted 2 to 3 times with 60% to 80% ethanol aqueous solution equivalent to 6 to 10 times of raw material weight, and the combined extracts reclaim ethanol;
(4), the extract of step C3) is spray-dried to obtain a dry powder, and then the dry powder is dissolved with 10%~30% ethanol aqueous solution, and centrifugally filtered;
(5), get the filtrate after the centrifugal filtration of step (4), concentrate and dry under reduced pressure in a water bath to obtain mangiferin.
Preferably, the mangiferin that step (5) obtains is recrystallized twice with 20%～50% ethanol solution to obtain the pure product of mangiferin.
Preferably, the enzyme solution in step (1) is selected from one or more of cellulase solution, pectinase solution and papain solution.
Preferably, the enzymolysis temperature in step (1) is 50 DEG C.
Preferably, the enzyme solution pH value in step (1) is 4.0～5.0.
Preferably, the pH value of the enzyme solution is 4.5.
Preferably, the concentration of the aqueous ethanol solution in step (3) is 75%.
Preferably, the concentration of the aqueous ethanol solution in the step is 20%.
Preferably, the concentration of the ethanol solution is 40%.
Preferably, the amount of enzyme added per gram of raw material is respectively 40 μ g to 140 μ g of cellulase, 20 μ g to 120 μ g of pectinase, and 1.5% to 7.5% of papain.
Compared with the prior art, the present invention adopts enzymatic method to extract mangiferin from mango leaves for waste utilization, greatly reduces production cost, not only improves the yield of the product, but also improves the purity of the product, and the whole production process has mild conditions, Simple and easy.
In the present invention, the mango leaf is first adjusted to the pH value, and then the active enzyme is added thereto, and the pH value is not adjusted by adding the enzyme first, which can effectively prevent the solution of adding the enzyme preparation from dripping a strengthened acidic solution, causing the solution to be localized. The acidity is too strong, so that the actual enzyme content in the solution is reduced.