1. Crush Centella asiatica, extract with 60-70v% ethanol option, filter the remove, recuperate the solvent from the filtrate, include 40-50C water to dissolve, filter, as well as liquify the filter residue in 50-60v% ethanol option to get Crude remove.
2. Place the crude extract on XAD-9 manhole material column, elute with acid remedy with pH value of 2-4, and combine the flow remedy and also acid clean remedy.
3. Change the pH worth of the combined remedy to nonpartisanship, filter, and also make use of the filtrate once again to EXAD-3 macroporous resin column, elute with ethanol with a volume focus of 30-40%, follow up discovery by thin-layer chromatography, as well as collect the parts having asiatic acid. ethanol eluent; re-blade volume concentration, 55-65% ethanol elution, thin layer, chromatographic tracking discovery, accumulate the ethanol eluent having Madecassic acid, recoup the Madecassoside eluent to ethanol, dry, to get Madecassoside.
Symphonious 1, ethanol with a quantity focus of 60-70% can totally liquify diterpenoids in Centella asiatica, consisting of triterpenoids and triterpenoids. Due to the fact that the particles of Centella asiatica are commonly combined with many sugar molecules, there are numerous hydroxyl groups for a few days, which can reveal a specific level of hydrophilicity, yet the foot hydrophilicity is not solid, as well as it is somewhat soluble in water, while diterpene acids are polar Tiny and also insoluble in water. Consequently, using 40-50 ℃ warm water can assist the dissolution of Centella asiatica total saponins, as well as the asiaticotritan acids are insoluble in hot water. Filter the filtrate, and also take the filter deposit as well as dissolve it in ethanol, so regarding achieve the everyday efficiency of removing Centella asiatica total saponins.
XAD-9 resin generally adsorbs fat-soluble elements and also substances with solid macromolecular polarity. These compounds have more powerful adsorption to resins. Macroporous resins preferentially adsorb these substances as well as promptly get to saturation. XAD-9 is a polar macroporous material, which is rather different in polarity from triterpene acids and also can not efficiently adsorb triterpenoids. The typical pore dimension of XAD-9 resin is larger than that of triterpenoids as well as can not be It plays the duty of physically keeping triterpene acids, so the triterpenoids will certainly flow out with the effluent. The acid option with pH value of 2-4 can even more elute a percentage of triterpenoid acids adsorbed in the material, as well as prevent the adsorption loss of triterpenoid acids.
In step 2, the column rate of the unrefined extract is 3-5BV/ h. The elution price of the acid option is 1-2 BV/h.
In step 3, the XAD-3 macroporous resin is a non-polar material with a typical pore dimension of 0.44 and a specific surface area of 256m2/g. The resin has very good adsorption efficiency for triterpenoid acids, and also the filling speed of the filtrate is 0.5-1BV/ h, which can entirely adsorb the diterpenoids. Due to the distinction in polarity between asiatic acid and Madecassoside, 30-40% ethanol by quantity was used to elute asiatic acid preferentially. Then it is eluted with 55-65% ethanol by quantity, and also the continuing to be Madecassoside can be eluted.
Compared to the prior art, the approach performs adsorption and also desorption for the target component, the return of the extracted Madecassoside is as high as 90% or more, as well as the purity is as high as 95.0% or more.