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Zhishang Chemical

Madecassoside CAS 18449-41-7

Molecular Formula: C30H48O6

Formula Weight: 504.71

ZSpharmac: Madecassoside Supplement

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Product Name: Madecassoside
CAS No: 18449-41-7
Purity: 99%

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Basic Info

Product Name:Madecassoside
Other Names:Madecassic Acid, 98%, From Centella Asiatica (L.) Urb.
CAS:18449-41-7
Place of Origin:Shandong, China
Brand Name:ZSpharmac
Type:Cosmetic Raw Materials
Appearance:White to Pale Red Solid
EINECS No.:0
Storage:2-8°C
Provide:Madecassoside MSDS;
Madecassoside COA

What is Madecassoside?

Madecassoside (madecassoside, MC) is the main active component of triterpenoid saponins in Centella asiatica Urb. of Umbelliferae. Studies have shown that Madecassic acid has a wide range of in vitro and in vivo pharmacological activities, which can reduce collagen-induced arthritis inflammation and promote in vitro Proliferation of cultured human fibroblasts, treatment or prevention of hypertrophic scars and keloids, and protection of myocardial ischemia-reperfusion injury. In addition, Madecassoside has antidepressant effect, and the mechanism may be related to reducing the activity of brain monoamine oxidase. At the same time, Madecassoside also has protective effect on neurons in rats with acute spinal cord injury and dementia in mice caused by chronic aluminum poisoning.

Madecassic Acid Properties:

Melting point 270°C (dec.)
Boiling point 641.7±55.0 °C(Predicted)
density 1.23±0.1 g/cm3(Predicted)
storage temp. 2-8°C
solubility DMSO (Sparingly), Methanol (Slightly, Heated)
pka4.63±0.70(Predicted)
form Solid
color White to Pale Red

 

Effects of Madecassoside

The wound area of the mice in the high-dose group of Madecassoside was significantly reduced, and the content of hydroxyproline in the skin tissue was significantly increased; H2O2 (500 μmol/L) caused a decrease in the activity of HUVEC, an increase in the leakage of LDH, and an increase in the content of intracellular MDA. , GSH content decreased, TUNEL test found that the apoptosis rate of H2O2 treatment group increased significantly. Madecassic acid (5, 10, 20μmol/L) can concentration-dependently inhibit lipid peroxidation and up-regulate cell viability; significantly improve H2O2-induced endothelial cell apoptosis and reduce intracellular Caspase-3 activity. Conclusion: Madecassoside has a significant promoting effect on burn healing, and is one of the main active components of Centella asiatica in the treatment of burns and scalds. Its mechanism of action is related to inhibiting lipid peroxidation, promoting collagen synthesis and inhibiting apoptosis.

Madecassoside can inhibit Aβ-induced apoptosis of hippocampal neurons; can inhibit myeloperoxidase (MPO) and reduce the production of malondi-aldehyde (MDA), thereby inhibiting inflammation and reducing oxygen free radical damage . LPS can significantly induce the proliferation and release of inflammatory factors of microglia in vitro. Compared with the LPS group, madecassoside significantly inhibited LPS-induced microglia proliferation in a dose-dependent manner. The IC50 of madecassoside treated microglia for 48h was 10.97nmol/L. At the same time, Madecassoside significantly decreased the release of TNF-α and IL-6 in microglia (P<0.05); Madecassic acid increased the rate of cell apoptosis in G2 phase of microglia and decreased TLR4 in microglia. and NF-κB expression.

COX-2 plays an important role in the process of inflammation, and its expression can be induced by endotoxin, mitogen, cytokine and so on. In acute liver injury caused by CCl4, the inflammatory response is activated, and various inflammatory factors including COX-2 are produced in large quantities, resulting in further necrosis of liver cells and aggravating the degree of liver injury. And it has been reported that COX-2 selective inhibitor can significantly reduce CCl4-induced liver injury Madecassoside can significantly inhibit the expression of COX-2 in liver tissue. This is one of the main mechanisms of liver protection.

The absorption of Madecassoside in the small intestine of rats is stronger than that of the large intestine, and its absorption rate is ileum>jejunum>duodenum>colon. However, there was no significant difference in the ileal absorption rate and absorption rate constant of Madecassoside solution with different concentrations. Among the three different surfactants, polysorbate-80 had a significant promoting effect on the ileal absorption of Madecassoside. The intestinal absorption of Madecassoside is mainly concentrated in the middle of the small intestine, and the absorption mechanism is speculated to be passive diffusion. The surfactant polysorbate-80 can promote its ileal absorption.

Extraction of Madecassoside

1. Crush Centella asiatica, extract with 60-70v% ethanol option, filter the remove, recuperate the solvent from the filtrate, include 40-50C water to dissolve, filter, as well as liquify the filter residue in 50-60v% ethanol option to get Crude remove.

2. Place the crude extract on XAD-9 manhole material column, elute with acid remedy with pH value of 2-4, and combine the flow remedy and also acid clean remedy.

3. Change the pH worth of the combined remedy to nonpartisanship, filter, and also make use of the filtrate once again to EXAD-3 macroporous resin column, elute with ethanol with a volume focus of 30-40%, follow up discovery by thin-layer chromatography, as well as collect the parts having asiatic acid. ethanol eluent; re-blade volume concentration, 55-65% ethanol elution, thin layer, chromatographic tracking discovery, accumulate the ethanol eluent having Madecassic acid, recoup the Madecassoside eluent to ethanol, dry, to get Madecassoside.

Symphonious 1, ethanol with a quantity focus of 60-70% can totally liquify diterpenoids in Centella asiatica, consisting of triterpenoids and triterpenoids. Due to the fact that the particles of Centella asiatica are commonly combined with many sugar molecules, there are numerous hydroxyl groups for a few days, which can reveal a specific level of hydrophilicity, yet the foot hydrophilicity is not solid, as well as it is somewhat soluble in water, while diterpene acids are polar Tiny and also insoluble in water. Consequently, using 40-50 ℃ warm water can assist the dissolution of Centella asiatica total saponins, as well as the asiaticotritan acids are insoluble in hot water. Filter the filtrate, and also take the filter deposit as well as dissolve it in ethanol, so regarding achieve the everyday efficiency of removing Centella asiatica total saponins.

XAD-9 resin generally adsorbs fat-soluble elements and also substances with solid macromolecular polarity. These compounds have more powerful adsorption to resins. Macroporous resins preferentially adsorb these substances as well as promptly get to saturation. XAD-9 is a polar macroporous material, which is rather different in polarity from triterpene acids and also can not efficiently adsorb triterpenoids. The typical pore dimension of XAD-9 resin is larger than that of triterpenoids as well as can not be It plays the duty of physically keeping triterpene acids, so the triterpenoids will certainly flow out with the effluent. The acid option with pH value of 2-4 can even more elute a percentage of triterpenoid acids adsorbed in the material, as well as prevent the adsorption loss of triterpenoid acids.

In step 2, the column rate of the unrefined extract is 3-5BV/ h. The elution price of the acid option is 1-2 BV/h.

In step 3, the XAD-3 macroporous resin is a non-polar material with a typical pore dimension of 0.44 and a specific surface area of 256m2/g. The resin has very good adsorption efficiency for triterpenoid acids, and also the filling speed of the filtrate is 0.5-1BV/ h, which can entirely adsorb the diterpenoids. Due to the distinction in polarity between asiatic acid and Madecassoside, 30-40% ethanol by quantity was used to elute asiatic acid preferentially. Then it is eluted with 55-65% ethanol by quantity, and also the continuing to be Madecassoside can be eluted.

Compared to the prior art, the approach performs adsorption and also desorption for the target component, the return of the extracted Madecassoside is as high as 90% or more, as well as the purity is as high as 95.0% or more.

Separation and Determination of Madecassoside

When the mobile phase is methanol-water (volume ratio of 65:35) and pH is 4, the separation degree of isomers increases with the increase of the concentration of β-cyclodextrin; Within the range of 5.0g/L, the peak area and concentration showed a linear relationship (r2=0.9989), indicating that the method is suitable for the content detection of Madecassoside and the quality control of related drugs. Madecassoside and its isomer termi-nolicacid were separated by adding β-cyclodextrin to methanol-water mobile phase, and the content of Madecassoside in 50% of total aglycones of Centella asiatica was determined. The specificity of this method is good, and the concentration of Madecassoside has a good linear relationship with the peak area when the concentration of Madecassoside is 0.1-5.0g/L. It can be used for the content detection of Madecassoside and the quality control of related drugs. Chromatographic conditions: Column: AgilentZorbaxSB-C18 column (150mm×4.6mm, 5μm); Guard column: ShimadzuShim-packODS; Mobile phase: methanol-water (volume ratio of 65:35) mixed solution (containing 4mmol/Lβ-CD), The pH value was adjusted to 4 with acetic acid; the flow rate was 0.4 mL/min; the detection wavelength was 204 nm; the column temperature was 25 °C; the injection volume was 10 μL.

Company Profile and Corporate Culture

Company Profile:

ZhiShang Chemical is owned by ZhiShang Group is a professional new-type chemicals enterprise combined into research and development, production and sales .

The company’s competitive product is pharmaceutical raw materials and intermediates (especially carbohydrate derivatives Series), In recent years, the company has made a major breakthrough in food and feed additives, plant extraction, industrial chemicals industry .

The company insists on the spirit of “sincere management, strict quality control, customer as god” , get consistent high praise from customers at home and abroad.

Corporate Culture:

OUR MISSION
Help China Chemicals to benefit the happiness of human life
OUR VISSION
Become the most trusted chemical supplier in the world
OUR BUSINESS PHILOSOPHY
Striver – oriented, enrich employees, customer first, deep service, seek development
OUR VALUES
Be prepared for danger in times of peace, forge ahead actively, unity and cooperation, and be brave to fight

About Us

The production base is located in Zhangqiu chemical industry park and Tai’an high-tech chemical industry park. laboratory and workshop in strict accordance with the GMP standard and the product fit national ISO9001 and ISO2000 standards.

“Zhishang” products are exported to Europe, North and South America, the Middle East, Asia Pacific and Africa area, so as to establish a long-term and stable cooperation relationship with customer in the world.

Company Info
  • Business Type: Manufacturer
  • Product Range: Additive , Chemical Auxiliary & Catalyst , Organic Chemicals
  • Products/Service: Organic Intermediate,Inorganic Chemistry, APIs, Dyestuffs And Pigments, Fragrance And Spices, Food Additives
  • Total Employees: 51~100
  • Capital (Million US $): 10000000RMB
  • Year Established: 2016
Production Capacity
  • No. of Production Lines : 8
  • No. of QC Staff : 5 -10 People
  • OEM Services Provided : yes
  • Factory Size (Sq.meters) : 3,000-5,000 square meters
  • Certificate: ISO9001 , CE , GMP , API , MSDS
  • Factory Location : Diao Town Industry Park, Zhangqiu City, Jinan City, Shandong Province, China.

Service

Pre-Sales Service

* Prompt reply and 24 hours online, professional team to provide best price and high quality product.

* Sample testing support.

* Every batch of products will be tested to ensureits quality.

*The packing also can be according the customers` requirment.

*Any inquiries will be replied within 24 hours.

*we provide Commerical Invoice, Packing List, Bill of loading, COA , Health certificate and Origin certificate. If your markets have any special requirements, let us know.

 

After-Sales Service

*The fact of logistics information monitoring.

* Any questions about the product can be consulted at any time.

*Product has any problem can return.

FAQ

Do you accept sample order?

We will make samples before mass production, and after sample approved, we’ll begin mass production. Doing 100% inspection during production, then do random inspection before packing.

 

HOW TO CONFIRM THE PRODUCT QUALITY BEFORE PLACING ORDERS?

You can get free samples for some products,you only need to pay the shipping cost or arrange a courier to us and take the samples. You can send us your product specifications and requests,we will manufacture the products according to your requests.

What’s your MOQ?

Our MOQ is 1kg. But usually we accept less quantity such as 100g on the condition that sample charge is 100% paid.

Do you supply product report?

Yes. We’ll give you product analysis report before shipping.

  Is there a discount?

Different quantity has different discount.

Shipping

1. ≤50kg, Express delivery recommended, usually called as DDU service;

2. ≤500kg, Air shipping recommended, usually called as FOB, CFR, or CIF service;

3. >500kg, sea shipping recommended, usually called as FOB, CFR, or CIF service;

4. For high value products, please select air shipping and express delivery for safe.

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