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Madecassic Acid CAS 18449-41-7

Molecular Formula: C30H48O6

Formula Weight: 504.71

ZSpharmac: Madecassic Acid Supplier

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Product Name: Madecassic Acid
CAS No: 18449-41-7
Purity: 99%

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Basic Info

Product Name:Madecassic Acid
Other Names:Madecassicacid
CAS:18449-41-7
Place of Origin:Shandong, China
Brand Name:ZSpharmac
Type:Cosmetic Raw Materials
Appearance:White to Pale Red Solid
EINECS No.:N/A
Storage:2-8°C
Provide:Madecassic Acid MSDS;
Madecassic Acid COA

What is Madecassic Acid?

Madecassic Acid is the main active ingredient of triterpenoid saponins in Centella asiatica Urb., and studies have shown that MC has a wide range of in vivo and in vitro pharmacological activities, which can reduce collagen-induced arthritis inflammatory responses and promote human fibroblasts cultured in vitro. Proliferation, treatment or prevention of hypertrophic scars and keloids, and protection of myocardial ischemia-reperfusion injury, etc. In addition, MC has an antidepressant effect, and the mechanism may be related to reducing the activity of brain monoamine oxidase; at the same time, MC also has a protective effect on neurons in rats with acute spinal cord injury and dementia in mice caused by chronic aluminum poisoning.

Madecassic Acid Property

Melting point 270°C (dec.)
Boiling point 641.7±55.0 °C(Predicted)
density 1.23±0.1 g/cm3(Predicted)
storage temp. 2-8°C
solubility DMSO (Sparingly), Methanol (Slightly, Heated)
form Solid
pka4.63±0.70(Predicted)
color White to Pale Red

 

Madecassic Acid Uses

Skin Damages Repair
The wound area of the computer mice in the Madecassic Acid high-dose group was significantly lowered, and the content of hydroxyproline in the skin tissue was dramatically raised; H2O2 (500 μmol/ L) triggered a reduction in HUVEC activity, enhanced LDH leak, raised intracellular MDA web content, GSH TUNEL assay discovered that the apoptosis price of H2O2 treatment group boosted significantly. Madecassic acid (5, 10, 20 μmol/ L) can inhibit lipid peroxidation in a concentration-dependent manner, up-regulate cell practicality; significantly boost H2O2-induced apoptosis of endothelial cells, as well as lower intracellular Caspase-3 task. Conclusion: Madecassic acid can substantially promote burn healing, and it is among the primary energetic components of Centella asiatica in treating burns and also scalds. Its system of action is related to inhibiting lipid peroxidation, promoting collagen synthesis as well as hindering apoptosis.

Liver security
COX-2 plays a crucial duty in the inflammatory process as well as can be induced by endotoxin, mitogens, cytokines and so on. In the acute liver injury caused by CCl4, the inflammatory action is activated, and different inflammatory variables including COX-2 are created in huge quantities, resulting in additional death of liver cells and also worsening the degree of liver injury. And also it has actually been reported that COX-2 discerning preventions can considerably lower the liver injury brought on by CCl4. Madecassic Acid can significantly inhibit the expression of COX-2 in liver cells. This is among the major mechanisms of hepatoprotective effect.

Intestinal absorption mechanism
The absorption rate of Madecassic Acid in the small intestine of rats is stronger than that in the huge intestine, as well as the absorption price is ileum > jejunum ≥ duodenum > colon. There was no considerable difference in the ileal absorption price and also absorption rate constant of various focus of madecassoside options. Among the 3 different surfactants, polysorbate-80 can dramatically advertise the ileal absorption of madecassoside. The digestive tract absorption of madecassoside is primarily focused in the middle of the small intestine, and also the absorption mechanism is assumed to be easy diffusion, and the surfactant polysorbate-80 can promote its ileal absorption.

Extraction of Madecassic Acid

1. Crush Centella asiatica, extract with 60-70v% ethanol solution in a stream, filter the extract, recover the solvent from the filtrate, add 40-50C water to dissolve, filter, and dissolve the filter residue in 50-60v% ethanol solution to obtain crude extract.

2. Put the crude extract on XAD-9 manhole resin column, elute with acid solution with a pH value of 2-4, and combine flow solution and acid wash solution.

3. Adjust the pH value of the combined solution to neutral, filter it, and refill the filtrate with EXAD-3 macroporous resin column, elute with ethanol with a volume concentration of 30-40%, track and detect by thin-layer chromatography, and collect components containing asiatic acid ethanol eluate; then volume concentration, 55-65% ethanol elution, thin layer, chromatography tracking detection, collection of ethanol eluate containing madecassic acid, the madecassic acid eluate Recover ethanol and dry to obtain madecassic acid.

In step 1, ethanol with a volume concentration of 60-70% can fully dissolve the diterpenoids in Centella asiatica, including triterpene saponins and triterpene acids. Because the molecules of centella asiatica soap are often combined with many sugar molecules, and the number of hydroxyl groups is large, it can show a certain degree of hydrophilicity, but the hydrophilicity is not strong enough, and it is slightly soluble in water, while diterpene acids are polar. Small and insoluble in water. Therefore, the use of warm water at 40-50°C can help the total saponins of Centella asiatica to dissolve, and the acid substances of centella asiatica are insoluble in hot water. Filter the filtrate, take the filter residue and dissolve it in ethanol to achieve the daily goal of removing total saponins of Centella asiatica. .

XAD-9 resin mainly adsorbs fat-soluble components and macromolecular substances with strong polarity. These substances have stronger adsorption to the resin, and the macroporous resin preferentially adsorbs these substances and quickly reaches saturation. XAD-9 is a polar macroporous resin, which has a large difference in polarity from triterpene acids, so it cannot effectively adsorb triterpene acids. It plays the role of physically intercepting triterpenic acid compounds, so the triterpene acid compounds will flow out with the effluent. The elution with acid solution with a pH value of 2-4 can further elute a small amount of triterpene acid compounds adsorbed in the resin to avoid the adsorption loss of triterpene acid compounds.

In step 2, the column speed of the crude extract is 3-5BV/h. The elution rate of acid solution is 1-2BV/h.

In step 3, the XAD-3 model macroporous resin is a non-polar resin with an average pore diameter of 0.44um and a specific surface area of 256m2/g. The resin has very good adsorption properties for triterpenic acid compounds, and the column loading speed of the filtrate is 0.5-1BV/h, which can completely adsorb diterpenic acid compounds. Due to the difference in polarity between Asiatic acid and Madecassic Acid, Asiatic acid can be preferentially eluted by ethanol with a volume concentration of 30-40%. Then elute with ethanol with a volume concentration of 55-65%, and the remaining Madecassic Acid can be eluted.

Compared with the prior art, this method performs adsorption and desorption on target components, and the yield of extracted Madecassic Acid is as high as 90%, and the purity is as high as 95.0%.

The Specific Implementation of Madecassic Acid Extraction

1. Crush Centella asiatica, extract with reflux of 60v% ethanol option, the dose of 60v% ethanol is 5 times of the weight of Centella asiatica, reflux remove at 40 ° C as soon as, each time for 0.5 h, filter the remove, Filtrate recovery solvent, include 40 ℃ of water once again to liquify, filter, as well as filter deposit is liquified in 50v% ethanol option, acquires crude remove.

2. Put the unrefined extract on the XAD-9 macroporous material column, the unrefined remove is filled on the column at a speed of 3BV/h, and is eluted with an acid option with a pH worth of 2, and the elution rate of the acid solution is 1BV/h, and the mixed circulation liquid as well as pickling solution.

3. Change the pH value of the combined remedy to neutral, filter, placed the filtrate on the XAD-3 macroporous resin column once more, the column speed of the filtrate is 0.5 BV/h, elute with ethanol with a quantity focus of 30%, and also laundry with ethanol The desorption degree is 2BV/h, complied with by TLC detection, and the ethanol eluate having Asiatic acid is gathered. Then elute with ethanol with a volume focus of 55%, and the ethanol elution rate is 2BV/h thin-layer chromatography tracking discovery, accumulate the ethanol eluate having madecassic acid, and also recover the ethanol from the madecassic acid eluate, Dry to get madecassic acid.

By HLPC evaluation, the recovery rate of Madecassic Acid was above 90.08%, as well as the pureness was as high as 95.27%.

About Products:
If you need Madecassic Acid, a cosmetic raw material, please contact our service staff directly, and we will provide you with high-quality products at the best price.
As an experienced manufacturer and supplier of Madecassic Acid, our company, ZSpharmac will provide Madecassic Acid raw materials for customers all over the world for a long time.
Over the years, the company has been adhering to the spirit of “integrity management, strict quality control, customer first”, and has won unanimous praise from domestic and foreign customers.

Company Profile and Corporate Culture

Company Profile:

ZhiShang Chemical is owned by ZhiShang Group is a professional new-type chemicals enterprise combined into research and development, production and sales .

The company’s competitive product is pharmaceutical raw materials and intermediates (especially carbohydrate derivatives Series), In recent years, the company has made a major breakthrough in food and feed additives, plant extraction, industrial chemicals industry .

The company insists on the spirit of “sincere management, strict quality control, customer as god” , get consistent high praise from customers at home and abroad.

Corporate Culture:

OUR MISSION
Help China Chemicals to benefit the happiness of human life
OUR VISSION
Become the most trusted chemical supplier in the world
OUR BUSINESS PHILOSOPHY
Striver – oriented, enrich employees, customer first, deep service, seek development
OUR VALUES
Be prepared for danger in times of peace, forge ahead actively, unity and cooperation, and be brave to fight

About Us

The production base is located in Zhangqiu chemical industry park and Tai’an high-tech chemical industry park. laboratory and workshop in strict accordance with the GMP standard and the product fit national ISO9001 and ISO2000 standards.

“Zhishang” products are exported to Europe, North and South America, the Middle East, Asia Pacific and Africa area, so as to establish a long-term and stable cooperation relationship with customer in the world.

Company Info
  • Business Type: Manufacturer
  • Product Range: Cosmetic raw materials
  • Products/Service:Cosmetic raw materials (flavors and fragrances,antioxidant products, moisturizing and hydrating, whitening agents, cosmetic additives)
  • Total Employees: 51~100
  • Capital (Million US $): 10000000RMB
  • Year Established: 2016
Production Capacity
  • No. of Production Lines : 8
  • No. of QC Staff : 5 -10 People
  • OEM Services Provided : yes
  • Factory Size (Sq.meters) : 3,000-5,000 square meters
  • Certificate: ISO9001 , CE , GMP , API , MSDS
  • Factory Location : Diao Town Industry Park, Zhangqiu City, Jinan City, Shandong Province, China.

Service

Pre-Sales Service

* Prompt reply and 24 hours online, professional team to provide best price and high quality product.

* Sample testing support.

* Every batch of products will be tested to ensureits quality.

*The packing also can be according the customers` requirment.

*Any inquiries will be replied within 24 hours.

*we provide Commerical Invoice, Packing List, Bill of loading, COA , Health certificate and Origin certificate. If your markets have any special requirements, let us know.

 

After-Sales Service

*The fact of logistics information monitoring.

* Any questions about the product can be consulted at any time.

*Product has any problem can return.

FAQ

Do you accept sample order?

We will make samples before mass production, and after sample approved, we’ll begin mass production. Doing 100% inspection during production, then do random inspection before packing.

 

HOW TO CONFIRM THE PRODUCT QUALITY BEFORE PLACING ORDERS?

You can get free samples for some products,you only need to pay the shipping cost or arrange a courier to us and take the samples. You can send us your product specifications and requests,we will manufacture the products according to your requests.

What’s your MOQ?

Our MOQ is 1kg. But usually we accept less quantity such as 100g on the condition that sample charge is 100% paid.

Do you supply product report?

Yes. We’ll give you product analysis report before shipping.

  Is there a discount?

Different quantity has different discount.

Shipping

1. ≤50kg, Express delivery recommended, usually called as DDU service;

2. ≤500kg, Air shipping recommended, usually called as FOB, CFR, or CIF service;

3. >500kg, sea shipping recommended, usually called as FOB, CFR, or CIF service;

4. For high value products, please select air shipping and express delivery for safe.

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