CN00128226.3 reported a method for industrial production of a high-concentration lycopene crystalline product with a Lycopin Red content of >10%.
1. Raw materials: There are two kinds of raw materials used in the production of Lycopin Red in the present invention, one of which is tomato paste, and the concentration ratio is 3 to 8:1 (referring to obtain 1 part of tomato paste from 3 to 8 parts of fresh tomatoes), and its lycopene content It is 40-70mg/100g, and is stored in large barrels and used for annual industrial production; the second is the leftovers separated from the beater in the production process of tomato paste-tomato peel residue, which is mainly composed of tomato peel and tomato peel. The content of lycopene in tomato seeds and tomato skin is about 35mg/100g.
②Removal of water-soluble components in tomato sauce: Add 1 to 5 times of water to the concentrated tomato sauce in 1 to 3 times, stir, and heat to maintain the temperature at 20 to 70 °C, so that the soluble components in the tomato sauce can be dissolved as much as possible In water, centrifuge to remove water and soluble substances dissolved in it such as sugar, organic acids, inorganic salts, pectin, etc. The removed juice is called tomato juice, and tomato juice can be processed for downstream products, such as Concentrating and preparing tomato beverages, etc.; the obtained tomato pulp residue is wet tomato paste residue, the components contained therein are mainly cellulose, oils, carotenoid pigments and other components, and the water content is 80%-90%;
If tomato peel residue is used as raw material, the moisture content of wet tomato peel residue discharged from the beater of the tomato paste production line is 80-93%, which is extremely perishable and deteriorated. The method of separating the peel and seeds after drying can be used; The method of separating the skin and seeds first, and then drying is adopted; in the present invention, the method of separating the skin and seeds first, and then drying is preferred. The specific operation is to add 2-5 times of water to the wet tomato skin residue, mix and stir evenly, pump Into the hydrocyclone, the tomato skin and seeds are separated by the difference in the sedimentation speed. Finally, the tomato skin is discharged from the upper part of the hydrocyclone, and the tomato seeds are discharged from the bottom of the hydrocyclone; the tomato skin passes through the centrifuge. Remove the attached moisture, enter the drying equipment to dry, preferably use the belt drying equipment to dry, the drying temperature is 50～80℃, the drying time is 0.3～1h, and the tomato skin is dried to the moisture content of 1.0～8.0%.
③Degreasing treatment of ketchup residue: add 1 to 3 times the amount of 0.5 to 5% (w/v%) lye to the ketchup residue. The alkali used is sodium hydroxide, sodium carbonate or other alkaline substances. Keep the temperature at 20-70°C for 5-30 minutes to dissolve the lipids and other components in the ketchup residue, centrifuge to remove the lye, rinse with clean water repeatedly, and centrifuge for 2-3 times. In the present invention, degreasing may not be used, but the oleoresin content in the lycopene extract is relatively high, which affects the crystallization and precipitation process of lycopene. In the present invention, it is preferable to treat tomato paste residue with alkali.
④Drying of tomato paste residue: Various conventional drying equipment can be used to dry tomato paste residue or tomato skin. It is preferable to use paddle drying equipment to dry the wet tomato residue to a moisture content of 60%-75%, and then use a belt drying method. Drying equipment, drying temperature is 50-80 ℃, drying time is 0.5-3h, and the tomato paste residue is dried to a moisture content of 3.0-12.0%, preferably a moisture content of 3-5%, and a lycopene content of 150-360mg/ 100g; cooled to room temperature, and stored in bags at a low temperature below 20°C, preferably at a temperature of 0-5°C, protected from light and oxygen; the dried tomato paste residue can be used as lycopene for a certain period of time. Raw material storage; wet tomato paste residue can also be directly used for lycopene extraction without drying.
⑤Pulverization: On the day of leaching, pulverize the dried tomato paste residue and sieve it so that the particle size is 20-60 mesh.
6. lycopene leaching: the present invention adopts organic solvents such as alcohol, solvent gasoline, ethyl acetate, ether, petroleum ether, hexane, acetone, etc. to leaching Lycopin Red, and the solvent to raw material ratio is 1.2～5.0: 1. The extraction temperature is 30 to 75°C, and 2 to 7 extractions are generally performed. The single extraction time is 20 to 60 minutes, and the stirring speed is 1 to 20 rpm; The leaching solvent is sucked into the tank by means of the vacuum degree in the tank; in the whole leaching process, inert gas, such as nitrogen, carbon dioxide, etc., is used for protection to prevent the loss of effective components;
⑦ Filter out the Lycopin Red extract and concentrate and crystallize: After the extraction is completed, filter out the extract containing lycopene from the extraction tank, and filter the extract with a high-density filter cloth larger than 200 mesh. The lycopene extract is filtered, sent to the flash tank, and concentrated by flash evaporation under the pressure of 200-2000Pa to remove > 2/3 of the organic solvent in the extract, and the extract is concentrated to make the lycopene in it The content of lycopene reaches more than 150mg/100ml, and at the same time, the temperature of the concentrated extract is rapidly reduced to -25℃～-10℃. At this temperature, the saturated solubility of lycopene is 40～60mg/100ml, which is in a supersaturated state, so The lycopene crystals are precipitated and sent to the crystallization tank to complete the crystallization;
Lycopin Red crystallization: in the low temperature crystallization tank of -5～25 ℃, the lycopene crystallization of > 60% contained in the concentrated extract is precipitated; the crystallization mother liquor is filtered to obtain lycopene crystals, and the crystals are at <200Pa) and drying to obtain a lycopene crystal product with a lycopene content of not less than 10%;
⑧ The obtained Lycopin Red crystalline product is stored in a sealed tank filled with inert gas (preferably nitrogen, carbon dioxide); or diluted and dissolved to prepare lycopene products of various types and required concentrations.