Method 1. Extract cholesterol and cephalin from the mind stem. The mind stem is soaked in 3 times of acetone for 20-24 hrs, mixed continuously, filtered, as well as the filtrate is used to prepare cholesterol. The filter cake is vacuum-dried, 2-3 times the quantity of ethanol is added, extracted 4-5 times, filtered, and also the precipitate is vacuum-dried to prepare cephalin, as well as the filtrate is vacuum-concentrated to acquire a concentrate.
Preparation of Lecithine Final product concentrate, include half the amount of ether, allow mean 2 hours, precipitate and filter to get a clear liquid, add acetone under fast mixing, precipitate, filter, accumulate the lotion, wash two times with acetone, vacuum completely dry, Obtained lecithin ended up product
Concentrate [diethyl ether] → [2h] made clear service [acetone] → lecithin ended up product.
Technique 2, utilizing sheep mind as basic material
Cholesterol was drawn out from fresh lamb mind, minced, immersed in acetone (3 times, 2.5 times, 1.5 times) for 3 times, 24 hr each time, mixed, filteringed system and pushed, the filtrate was made right into cholesterol, as well as the filter cake was dried out.
Essence, dissolve, focus the filter cake and include ether (3 times the quantity, 2.5 times the quantity, 1.5 times the amount) to remove 3 times, each 24h, stir, filter to obtain the filtrate, concentrate to acquire the concentrate Add a percentage of ethanol, heat to dissolve, speed up in a cold room for 24 hours, accumulate the precipitate, soak in ethanol for 3-4 times in a cool place, 3 times the quantity each time, heat to dissolve, cool down, speed up for 24 hours, and also pour out the supernatant. The supernatant was focused in vacuo to acquire a concentrate.
Filter cake [diethyl ether] → filtrate [concentration] → concentrate [ethanol] → supernatant [focus] → concentrate.
Prep Work of Lecithine End product Concentrate was included with half the quantity of ether under continuous mixing, represented 2h, filtered to obtain ether clear liquid, included ether clear liquid to acetone under fast stirring, sped up out, filteringed system, as well as cleaned the precipitate with acetone twice, vacuum cleaner drying to get lecithin ended up item
Concentrate [ether] → clear liquid [acetone] → lecithin completed product.
Method 3. Using yeast as basic material
Preparation of paste: Pass completely dry yeast powder via a 60-80 mesh filter, include 3 times the quantity of 82% -84% ethanol to remove for 18-24 hrs, maintain 70 ° C for 3 hrs, maintain stirring, allow cool down to below 30 ° C and filter, filter deposit After that essence twice, shake off ethanol, integrate the removes, concentrate in 70 ℃ vacuum cleaner, the concentration time is not greater than 24h, get paste. The filter deposit is used to extract ribonucleic acid as well as zymosan.
Dry yeast or Geotrichum candidum [ethanol, initially 70 ℃, after that 30 ℃] → extract [70 ℃] → paste.
Preparation of concentrate: Include 5% -10% water to the paste, include 3-5 times the amount of ether, mix vigorously for 2-3h, let represent 16-20h, throw out the center as well as reduced layers, and also save the top ether at -5 ° C Area in the fridge for 20-24h, the ergosterol crystallizes out, filter, boil down the filtrate, recover about 1/2 ether, then position it in the -5 ° C fridge for 18-22h, include anhydrous salt sulfate, filter to get ether filtrate, and distill Add regarding 2/3 ether to get a concentrate.
Paste [WATER, ether] → [Na2SO4] filtrate [purification] → concentrate.
Prep work of ethanol supernatant concentrate Add 3-5 times the amount of acetone, stir while adding, leave for some time after including, pour out the mixed remedy of ether as well as acetone, clean with anhydrous acetone 3-4 times to acquire a precipitate, include 2 times the amount of anhydrous ethanol, heated to 70 ℃, stirred for 1-2 hrs to dissolve, and entrusted to stand in the cold store to pass the fluid. The ethanolic supernatant was decanted and also precipitated as crude coagulation.
Prep Work of Lecithine End Product Distill the ethanol supernatant, recuperate the ethanol, obtain the precipitate, include anhydrous ether, stir, liquify, allow stand for 7 days, take in the supernatant, add 1.5 times the amount of acetone to speed up the precipitate, pour out the acetone, clean Precipitate 3-4 times, add ethanol to liquify at 70 ° C, as well as dry to get the ended up item of lecithin.
Ethanol supernatant [distillation] → precipitate [diethyl ether] → supernatant [acetone, ethanol] → lecithin ended up product.
Technique 4. Using the mind and spine as raw materials.
Extraction of cholesterol and also Lecithine Take fresh or frozen mind or spine, remove membranes and also blood veins and other impurities, mince them, impregnate them with 1.2 times the quantity of acetone for 4.5 hrs, mix continually, filter, and also repeat for 5 times, and the filtrate is utilized to prepare cholesterol. The filter cake was vacuum-dried, 2-3 times the amount of ethanol was added, extracted at 35-40 ° C. for 12 h, filtered, and also removed once again, the filter cake was vacuum-dried to prepare cephalin, and the filtrate was made use of to prepare lecithin.
Preparation of Precipitate Concentrate the filtrate to 1/3 quantity in vacuo to obtain a concentrated service, placed it in a chilly space overnight, filter, add a saturated service of cadmium chloride to the filtrate, speed up, filter, gather the precipitate, add 2 times the quantity of ether, shake, The ether was gotten rid of by centrifugation and also this was duplicated 8-10 times to gather the precipitate.
Filtrate [concentrated] → focused remedy [cadmium chloride, ether] → precipitate.
Prep work of concentrate Include 4 times the quantity of chloroform to the precipitate, tremble to acquire a remedy (turbid), include 25% ammonia methanol solution, precipitate, centrifuge, and focus the centrifuge in vacuum to dry skin to get a concentrate.
Precipitate [Chloroform] → Liquifying solution [Ammonia methanol service] → Concentrate.
Prep Work of Lecithine Finished product Concentrate is included with anhydrous ether, concentrated in vacuo, repeated two times to remove water to obtain a concentrate, add a small amount of ether, put it right into acetone, stand, filter, accumulate the precipitate, vacuum cleaner completely dry to obtain lecithin ended up item.
Concentrate [anhydrous ether] → concentrate [diethyl ether, acetone] → Lecithine ended up product.
