Preparation of Submicron Emulsions with Improved Efficacy:
A: According to the mass ratio of 5~10:3~5:5~10:1~3:0.3~0.5:10~20, weigh white pond flower seed oil, behenic acid, ceramide III synthesized from sphingosine and oleic acid , sugars, catalysts, deionized water, as the reaction bottom material into the stainless steel reactor;
B: Fill the stainless steel reaction kettle with nitrogen for protection. The temperature in the stainless steel reaction kettle is controlled at 120°C~140°C, the pressure is 1.2~1.8kgf/cm2, and the reaction time is 2~4 hours; the speed of scraping at 15rpm is kept during the reaction process. while stirring;
C: Take a small amount of the reactant product and analyze it by chromatographic analysis. The mass fraction of ultra-long carbon chain ceramide containing behenic acid group in the reaction product is higher than 2%, and the mass fraction of glycosylceramide is higher than 1%. That is to say, the reaction is considered complete; then the temperature in the stainless steel reactor is lowered to 75~90°C, and the pressure is reduced to normal pressure;
D: Add hydrogenated lysolecithin with a mass ratio of 1.0-3.0:100 to the reaction product, cholesterol with a mass ratio of 3.0-5.0:100, and deionized water with a mass ratio of 5.0-10.0:100 to the stainless steel reactor. The temperature in the stainless steel reaction kettle is 75~90℃, and the stirrer is scraped and stirred at 15rpm for 0.5~1 hour to form a uniform emulsion;
E: Use a high-pressure homogenizer to homogenize the emulsion obtained in step D three times at 75-90°C under a pressure of 1500-2500 bar, and cool to room temperature to obtain a submicron emulsion that can improve atopic dermatitis.
The submicron emulsion prepared by this method has the effect of improving atopic dermatitis, contains unreacted ceramide III synthesized from sphingosine and oleic acid, sugar components and behenic acid, and white pond flower seed oil, and contains reactive The product, that is, the ultra-long-chain ceramide containing behenic acid group generated by transesterification, and the glycosylceramide component generated by esterification, finally added cholesterol, hydrogenated lysolecithin as emulsifier, and deionized Water forms an emulsion, and after being homogenized by a high-pressure homogenizer, a nano-lipid particle emulsion encapsulated by cholesterol and hydrogenated lysolecithin is formed, and the average particle size of the nano-lipid particle in the obtained nano-lipid particle emulsion is 100-350nm.