Zhishang Chemical

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Zhishang Chemical

L-tert-Leucine CAS 20859-02-3

Molecular Formula: C6H13NO2

Formula Weight: 131.17

ZSpharmac: L-tert-Leucine Supplement

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Product Name: L-tert-Leucine
CAS No: 20859-02-3
Purity: 99%

Basic Info

Product Name:L-tert-Leucine
Other Names:L-tert.Leucine
CAS:20859-02-3
Place of Origin:Shandong, China
Brand Name:ZSpharmac
Type:Organic Intermediate
Appearance:White to Almost White Powder
EINECS No.:200-522-0
Storage:Keep in Dark Place,Sealed in Dry,Room Temperature
Provide:L-tert-Leucine MSDS;
SigmaAldrich COA

What is L-tert-Leucine?

L-tert-leucine is a non-polar amino acid whose tert-butyl team has significant hydrophobicity as well as steric hindrance, which can well manage the molecular conformation in chain reactions and also develop chiral substances. For that reason, L-tert-leucine is commonly made use of as an inducer or design template in uneven synthesis.Additionally, L-tert-leucine is an important additive in the food and also cosmetic markets.

In the early studies, chemical preparation of L-tert-leucine has problems such as low stereoselectivity, large pollution, complicated process and low yield. At present, the enzymatic method has gradually replaced the chemical method. Several previous studies on the production of L-tert-leucine using hydrolases, acyltransferases and amino acid dehydrogenases] have been reported. Among them, the use of NADH-dependent L-leucine dehydrogenase (LeuDH, EC 1.4.1.9) to prepare L-tert-leucine has the most industrial potential. NAD+-dependent formate dehydrogenase (FDH, EC 1.2.1.2) converts formate to CO2 while producing NADH. Therefore, FDH is usually used in coenzyme regeneration systems. Among all FDHs from different sources, FDH from Candida boidinii (Cb FDH) has been widely used for regeneration of NADH and has been demonstrated on a technical scale. At present, the coupling of LeuDH and Cb FDH to form a coenzyme regeneration system to prepare L-tert-leucine is the focus of research. This method enables continuous regeneration of NAD+ and reduces costs, and Degussa has successfully applied the LeuDH and FDH coupling system to produce L-tert-leucine on a large scale.
In a previous study, we identified a novel LeuDH from the halophilic thermophile Laceyella sacchari (Ls LeuDH). Ls LeuDH showed good thermal stability and great potential to synthesize L-tert-leucine in preliminary studies. Therefore, in this paper, we constructed a recombinant E. coli strain for co-expression of Ls LeuDH and Cb FDH, forming a two-enzyme system for the conversion of TMP to L-tert-leucine.

L-tert-Leucine Properties:

Melting point ≥300 °C (lit.)
alpha 6.3 º (c=4, 6 N HCl 200 ºC)
Boiling point 217.7±23.0 °C(Predicted)
density 1.1720 (estimate)
refractive index -9 ° (C=3, H2O)
storage temp. Keep in dark place,Sealed in dry,Room Temperature
solubility 1 M HCl: 50 mg/mL
pka2.39±0.12(Predicted)
form powder
color White to almost white
optical activity[α]20/D 9.5°, c = 3 in H2O
Water Solubility 125.5 g/L (20 ºC)

 

L-tert-Leucine Uses

  1. L-tert-leucine can be used as a catalyst for the enantioselective oxidative coupling and cyclization of hydroquinones to oxa[9]helixene.
  2. L-tert-leucine is made use of as a nutritional fortifier, pet feed additive, and also used in the synthesis of medicines.
  3. L-tert-leucine utilized as an amino acid is the basic element of healthy protein, and also among its main physiological functions is as a resources for protein synthesis. Occurs in the totally free or bound state in vivo. Proteins in the body are broken down to generate the following amino acids: Alanine, Arginine, Aspartic Acid, Asparagine, Cysteine, Lysine, Methionine, Phenylalanine, Serine, Threonine, Chromine Amino acid, tyrosine, valine.

Production Method of L-tert-leucine​

Unnatural amino acids and their derivatives are important chemical intermediates. Among them, L-tert-leucine is an important unnatural amino acid. It can be used for synthesizing pharmaceutical intermediates, as a chiral inducer for asymmetric synthesis and a resolving reagent for asymmetric resolution, and is a chiral precursor for synthesizing a variety of anti-AIDS drugs and hepatitis virus inhibitors. With the development of my country’s social economy and the enhancement of environmental protection awareness, the pharmaceutical and chemical industry not only needs to improve the production capacity of L-tert-leucine, but also needs to create a green and pollution-free production process. Therefore, the use of enzymatic biotransformation to produce L-tert-leucine is being paid more and more attention by relevant institutions. Divided from the production route, the methods for producing L-tert-leucine mainly include chemical reagent separation method, chiral source synthesis method, chemical synthesis method and biotransformation method. Among them, the resolution method is limited by the yield, the chiral source method is limited by the production capacity of natural products, and the cost of chemical synthesis method is high, so other synthetic methods have no successful large-scale application examples. The main method of leucine. In biotransformation method, utilize lipase to split racemate DL-tertiary leucine relevant technological research is more, such as Chinese patent CN201010210770.0 etc., but this route has the same defect as chemical splitting method, namely receive. rate cannot exceed 50%. Therefore, the main research directions of biological methods are currently focused on the route of reducing trimethylpyruvate to L-tert-leucine using L-leucine dehydrogenase, and using formate dehydrogenase to regenerate coenzyme NADH in situ, Ammonium formate is used to provide ammonia molecules and hydrogen atoms. As early as the 1990s, Germany’s Degussa Company used this route to combine with an ultrafiltration membrane reactor to produce L-tertiary leucine. Several patent applications have been disclosed for this route, such as Chinese patents CN201110116253.1, CN201110277030.3, etc. The main problems of its existence are as follows: 1. In fact, the L-tert-leucine dehydrogenase recognized by the academic community will be significantly inhibited by the substrate when the substrate concentration is too high, and the reaction rate will drop sharply (Enzyme Catalysis in Organic Synthesis EditionI, Wiley, 1186). ( J. Mol. Catal: B 1998, 5: 1-11). It can be seen that the above patent The method is inefficient or difficult to repeat. 2. Enzyme feeding amount is too much. Obviously, this is an inevitable consequence of problem I. For example, in the patent CN201110116253.1, the expensive coenzyme NAD feeding amount reaches 1% (w/w) of the substrate, and the economy is poor, and the concentration that the whole cell catalyst accounts for the reaction system is 80% (4g/5 mL) in the patent CN201110277030.3 3. There is no clear post-extraction process. Also due to the existence of problem I, its yield and yield cannot meet the needs of industrialized production. Based on the above three points, there is no report on the large-scale production of L-tert-leucine using this method. In order to solve the above issues, the item of the present creation is to offer a technique for enzymatically manufacturing L-tert-leucine. The technique has light preparation problems, is eco-friendly, has high return and also affordable, and also appropriates for commercial production. In order to attain the above purpose, the technical plan provided by the innovation is: a production approach of L-tertiary leucine, the technique makes use of L-leucine dehydrogenase as well as formate dehydrogenase to produce L-tertiary leucine, Its preparation response formula is:

a production approach of L-tertiary leucine, the technique makes use of L-leucine dehydrogenase as well as formate dehydrogenase to produce L-tertiary leucine, Its preparation response formula is: Wherein, LeuDH is leucine dehydrogenase; NADH is minimized nicotinamide adenine dinucleotide, particularly reductase coenzyme; NAD is oxidized nicotinamide adenine dinucleotide, particularly dehydrogenase coenzyme; FDH is formate dehydrogenase.

Company Profile and Culture

ZhiShang Chemical is owned by ZhiShang Group is a professional new-type chemicals enterprise combined into research and development, production and sales .

The company’s competitive product is pharmaceutical raw materials and intermediates (especially carbohydrate derivatives Series), In recent years, the company has made a major breakthrough in food and feed additives, plant extraction, industrial chemicals industry .

The company insists on the spirit of “sincere management, strict quality control, customer as god” , get consistent high praise from customers at home and abroad.

OUR MISSION

Help China Chemicals to benefit the happiness of human life

OUR VISION

Become the most trusted chemical supplier in the world

OUR BUSINESS PHILOSOPHY

Striver – oriented, enrich employees, customer first, deep service, seek development

OUR VALUES

Be prepared for danger in times of peace, forge ahead actively, unity and cooperation, and be brave to fight

About Us

The production base is located in Zhangqiu chemical industry park and Tai’an high-tech chemical industry park. laboratory and workshop in strict accordance with the GMP standard and the product fit national ISO9001 and ISO2000 standards.

“Zhishang” products are exported to Europe, North and South America, the Middle East, Asia Pacific and Africa area, so as to establish a long-term and stable cooperation relationship with customer in the world.

Company Info
  • Business Type: Manufacturer
  • Product Range: Additive , Chemical Auxiliary & Catalyst , Organic Chemicals
  • Products/Service: Organic Intermediate,Inorganic Chemistry, APIs, Dyestuffs And Pigments, Fragrance And Spices, Food Additives
  • Total Employees: 51~100
  • Capital (Million US $): 10000000RMB
  • Year Established: 2016
Production Capacity
  • No. of Production Lines : 8
  • No. of QC Staff : 5 -10 People
  • OEM Services Provided : yes
  • Factory Size (Sq.meters) : 3,000-5,000 square meters
  • Certificate: ISO9001 , CE , GMP , API , MSDS
  • Factory Location : Diao Town Industry Park, Zhangqiu City, Jinan City, Shandong Province, China.

Service

Pre-Sales Service

* Prompt reply and 24 hours online, professional team to provide best price and high quality product.

* Sample testing support.

* Every batch of products will be tested to ensureits quality.

*The packing also can be according the customers` requirment.

*Any inquiries will be replied within 24 hours.

*we provide Commerical Invoice, Packing List, Bill of loading, COA , Health certificate and Origin certificate. If your markets have any special requirements, let us know.

 

After-Sales Service

*The fact of logistics information monitoring.

* Any questions about the product can be consulted at any time.

*Product has any problem can return.

FAQ

Do you accept sample order?

We will make samples before mass production, and after sample approved, we’ll begin mass production. Doing 100% inspection during production, then do random inspection before packing.

 

HOW TO CONFIRM THE PRODUCT QUALITY BEFORE PLACING ORDERS?

You can get free samples for some products,you only need to pay the shipping cost or arrange a courier to us and take the samples. You can send us your product specifications and requests,we will manufacture the products according to your requests.

What’s your MOQ?

Our MOQ is 1kg. But usually we accept less quantity such as 100g on the condition that sample charge is 100% paid.

Do you supply product report?

Yes. We’ll give you product analysis report before shipping.

  Is there a discount?

Different quantity has different discount.

Shipping

1. ≤50kg, Express delivery recommended, usually called as DDU service;

2. ≤500kg, Air shipping recommended, usually called as FOB, CFR, or CIF service;

3. >500kg, sea shipping recommended, usually called as FOB, CFR, or CIF service;

4. For high value products, please select air shipping and express delivery for safe.

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