L-Serine Supplement CAS 56-45-1

Chemical synthesis
The chemical synthesis process route of L-Serine Supplement mainly includes copper glycine method, α-isoacetaldehyde amine method, acrylonitrile method and dichloropropionitrile method, etc. The raw materials used in chemical synthesis are relatively cheap, the process route is relatively simple, and it is easy to produce on a large scale, but the product synthesized by this method is DL-serine, so further resolution is required to obtain L-serine, which will greatly improve The cost of producing L-serine by chemical synthesis affects large-scale industrial production. The following is a brief introduction to the chemical synthesis process of L-serine by the copper glycine method:

Proteolysis
Some proteins in nature (such as the content of L-serine in silk reaches 13.64%) are rich in a large amount of L-Serine Supplement, so through proteolysis, separation and purification can obtain relatively pure L-serine. This method was widely used in the early production of L-serine, but the method is complicated to operate, produces a large amount of polluted water, and there are many types of amino acids in the product, and the cost of separation and purification is relatively high, so it is gradually favored by other L-serine production methods replace.

Enzyme method
Compared with the chemical method, the enzymatic method has a simpler reaction process, the product is easy to extract, and there are fewer by-products. The specific reaction is:

At present, the key to the enzymatic production is whether the serine hydroxymethyltransferase (SHMT) with excellent characteristics can be obtained. Serine hydroxymethyltransferase (SHMT) with high enzymatic activity was screened from nature, and its enzymatic activity, substrate tolerance and enzyme stability were improved through exogenous expression, site-directed mutation and other techniques. This is also the main trend of bio-enzymatic production of L-serine. However, subject to the catalytic ability of the enzyme, the production of L-serine by this method is still in the stage of laboratory research.

Microbial Fermentation
The use of cheap carbon sources for microbial fermentation to produce L-Serine Supplement has the advantages of low cost and low environmental pollution, which has attracted the attention of researchers at home and abroad. However, this method has not yet been industrialized due to problems such as low yield and high production cost. This is partly because L-serine is an intermediate metabolite, which cannot be effectively accumulated in the cell, and the synthesized L-serine can be effectively degraded into other products; in addition, the flow of sugar metabolism into L-serine synthesis is relatively small . With the development of genetic engineering technology, the use of metabolic engineering to modify microorganisms to efficiently produce target products has become the mainstream. With the help of newly developed microbial technology, there are more and more reports on the production of L-serine by microbial fermentation, and the advantages of L-serine produced by fermentation are becoming more and more prominent.

The breeding of L-Serine Supplement high-yielding strains by system metabolic engineering has gradually become the focus of breeding L-Serine high-yielding strains in the past two years. In recent years, there have been many successful cases of using systematic metabolic engineering to construct L-serine production strains: