Zhishang Chemical

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Zhishang Chemical

L-Serine CAS 56-45-1

Molecular Formula: C3H7NO3

Formula Weight: 105.09

ZSpharmac: L-Serine Supplier

Product Name: L-Serine
CAS No: 56-45-1
Purity: 99%

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Basic Info

Product Name:L-Serine
Other Names:L-Silk Amino Acids
Place of Origin:Shandong, China
Brand Name:ZSpharmac
Type:Cosmetic Raw Materials
Appearance:White Powder
EINECS No.:200-274-3
Provide:L-Serine MSDS;
L-Serine COA

What is L-Serine?

L-serine is an amino acid essential for the synthesis of phosphatidylserine, which belongs of the membrane layer layers of mind cells (i.e. nerve cells). It can be created in the body, including the mind, yet an outside supply in the diet is vital to preserve the required levels. Although preclinical researches have disclosed that L-serine can reduce brain swelling, L-serine degrees in human beings do not seem connected with mental deterioration or cognitive decrease. Thinking about that L-serine is a normally happening amino acid, supplementing in percentages is most likely safe.

L-Serine Property

Melting point 222 °C (dec.) (lit.)
alpha 15.2 º (c=10, 2N HCl)
Boiling point 197.09°C (rough estimate)
density 1.6
refractive index 1.4368 (estimate)
Fp 150°C
storage temp. 2-8°C
solubility H2O: 50 mg/mL
form powder
pka2.19(at 25℃)
color White
PH5-6 (100g/l, H2O, 20℃)
optical activity[α]20/D +13.5±0.5°, c = 5% in 5 M HCl
Water Solubility 250 g/L (20 ºC)


L-Serine Uses

  1. L-serine is made use of as a biochemical reagent and artificial additive.
  2. L-serine is made use of in biochemical research, preparation of cells culture medium, and also as amino acid dietary medication in medication.

L-Serine Production Method

Method 1. Hydrolysis method
Acid hydrolysis was carried out with silkworm cocoon coat as raw material, and then ion-exchanged water was used for separation and purification.
Process: Cocoon coat [acid hydrolysis]→[HCl, 110℃, 24h] hydrolyzate [deacidification, decolorization]→[732 resin, ammonia water, pH3.5-8] eluent [fractionation]→[717 resin] ] Collection solution [concentration, crystallization, purification] → [thin film evaporation] → L-serine
For acid hydrolysis, put 30kg of cocoon coat in the hydrolysis tank, add 150L of HCl with a concentration of 6mol/L, stir and heat at 110℃ for 24h, cool down to below 60℃, filter again, and wash the filter residue with pure water with five times the amount of filtrate. , the washings were combined into the filtrate to obtain an aqueous solution of about 800L.
Deacidification and deliquoring The hydrolyzate 100L is processed into H+ type 732 cation exchange resin column (150mm×2000mm polyvinyl chloride, two each with 25L of internal resin) from top to bottom with the flow rate of 100-120ml/min, and then use Pure water washes away the pigment to a clear solution without Cl-. Next, ammonia water with a concentration of 0.3 mol/L is eluted through the column from top to bottom at a flow rate of 80-100 ml/min until the amino acid flows out, and the eluate in the range of pH 3.5-8 is collected. Finally, the tyrosine was washed with 1 mol/L ammonia water, and the tyrosine was obtained by de-ammonia concentration and crystallization.
The above-mentioned eluent is obtained by fractionation and conventionally treated as 4 OH-type 717 anion exchange resin columns:
The first one is 150mm×2000mm, with 24L resin (polyvinyl chloride) inside
The second one is 150mm×1800mm, with 22L resin (polyvinyl chloride) inside
The third one is 150mm×1600mm, with 20L resin (polyvinyl chloride) inside
The fourth one is 150mm×1400mm, with 10L resin (polyvinyl chloride) inside
First, adjust the eluent to pH 7-8 with 1 mol/L NaOH solution, go to the first column at a flow rate of 120-150 ml/min, wash with pure water until the resin is saturated, and then connect the first and second columns in series , eluted with 0.1mol/L HCl, the flow rate is about 80-100ml/min, when the effluent has amino acids, it starts to collect, a total of 25 bottles (1000ml/bottle) are collected, and then the third column is connected in series, and the collection is performed when amino acids flow out. 50L, discard the 1st column. Connect the fourth column in series, and continue to elute until the effluent contains amino acids and collect until pH 2-3, about 50 bottles. Paper chromatography was performed on the pools of each group, and the pools containing serine were combined.
Concentration, crystallization and purification The above-mentioned combined solution is concentrated by thin-film evaporation until crystals appear. After cooling, about 2 times the amount of absolute ethanol is added and placed in a refrigerator overnight. The crystals are separated out, filtered, and dried to obtain L-serine. Calculated by cocoon coat, the yield is about 4%.

Method 2. Fermentation method with the addition of precursors
The metabolism of L-serine in the body is extremely fast, and it is difficult to produce it by direct fermentation. Generally, the fermentation method with the addition of precursors is generally used. The added precursors are mainly glycine, glycine trimethylidene or glyceric acid, and the industrialization of glycine as the precursor is carried out. Producers can be divided into two categories: heterooxotrophic and methylotrophic bacteria.
Heterotype strain, glycine produces L-serine
Glycine [Glycinophilic acid Corynebacterium or Nocardia butanes or Sarcinus albicans] → [fermentation] L-serine methylotrophic strain, glycine produces L-serine
Glycine [Pseudomonas or Mycobacterium or methanol-assimilating bacteria Arthrobacter sphaeroides] → [fermentation] L-serine

Method 3. Chemical synthesis method DL-serine can be synthesized by using hydroxyacetaldehyde as a raw material, and then split to obtain L-serine.
Synthetic method using glycolaldehyde as raw material
Synthetic method using diethyl bromomalonate as raw material
Synthetic method using vinyl compounds as raw materials

Method four, enzymatic method
Using the intermediate DL-2-oxooxazolidine-4-carboxylic acid (DL-OOC) in the chemical synthesis of DL-serine as raw material, using L-OOC hydrolase produced by Pseudomonas testosteroni or OOC produced from Bacillus subtilis Racemase action to produce L-serine. DL-2-oxooxazolidine-4-carboxylic acid (DL-OOC) [L-OOC hydrolase or racemase] → L-serine.

L-Serine Regulates Microglial Polarization

Microglia are immune cells in the central nervous system that function likewise to macrophages. After neurological conditions, the activation of glial cells plays a vital role in the inflammatory feedback (. Microglia are major players in the inflammatory response, as well as numerous signals in the microenvironment of the damaged location polarize macrophages in a time-dependent manner. cells/microglia; that is, after macrophages/microglia transiently polarize to the M2 phenotype, they change to the much more consistent M1 phenotype. M1 microglia produce numerous pro-inflammatory factors, reactive oxygen types generate cell death and also cell particles accumulation, thereby raising neuronal cell death; however, M2 microglia produce anti-inflammatory aspects as well as phagocytose cell debris for neuronal cell repair service. In the beginning of mind injury, microglia are in a state of hyperactivation, even more M1 microglia secrete a range of pro-inflammatory elements, rise nerve cell fatality, which is not conducive to the healing of motor cognitive feature after neurological conditions. As a result, if the number of M2 microglia significantly goes beyond that of M1 microglia The variety of cells can minimize neurotoxicity.

Genome-wide microarray evaluation of differentially shared genes complying with microglial inflammatory stimulation and L-serine therapy showed that L-serine particularly enhances peroxisome proliferator-activated receptor gamma (PPAR-γ) genetics expression. PPAR-γ is a vital transcription variable controling the polarization of macrophages and microglia. After brain injury, activation of PPAR-γ induces polarization of M1 microglia toward the M2 phenotype, raising the proportion of M2 microglia at the onset of injury. For that reason, hindering the release of inflammatory aspects and boosting the impact of anti-inflammatory variables can alleviate extreme inflammatory feedbacks, rescue harmed afferent neuron, as well as minimize neurotoxicity. Inhibition of Akt also affects genetics expression in M2 microglia. Lots of neuroprotective medications (neuroprotectants, complimentary extreme scavengers, and also growth variables) promote the repair of cognitive function in the brain by activating the Akt signaling pathway. L-serine can additionally turn on the PI3K path, a crucial action in the polarization of microglia towards the M2 phenotype in response to healthy protein A or IL-4. At the same time, L-serine might also activate PPAR-γ with the P13K/Akt/mTOR path, generate the polarization of microglia from the damaging M1 phenotype to the helpful M2 phenotype, and also hinder the cascade result of inflammatory conciliators. Rises phagocytosis of mobile particles as well as hinders neurotoxicity, advertising recovery from neurological conditions. In the co-culture of microglia and also oligodendrocytes in a white matter demyelination version established artificial insemination, we found that L-serine inhibited the secretion of inflammatory factors (TNF-α as well as IL-1β) and promoted the repair work of neural feature. It has actually also been shown that L-serine therapy advertises the spreading of oligodendrocyte forerunner cells at the body level, suggesting that L-serine decreases the level of white issue damages by managing the polarization of microglia after CNS injury.

About Products:
If you need L-Serine, a cosmetic raw material, please contact our service staff directly, we will provide you with high-quality products at the best price.
As an experienced L-Serine manufacturer and supplier, ZSpharmac will provide L-Serine raw materials to customers all over the world for a long time.
Over the years, the company has been adhering to the spirit of “integrity management, strict quality control, and customer first”, and has won unanimous praise from domestic and foreign customers.

Company Profile and Corporate Culture

Company Profile:

ZhiShang Chemical is owned by ZhiShang Group is a professional new-type chemicals enterprise combined into research and development, production and sales .

The company’s competitive product is pharmaceutical raw materials and intermediates (especially carbohydrate derivatives Series), In recent years, the company has made a major breakthrough in food and feed additives, plant extraction, industrial chemicals industry .

The company insists on the spirit of “sincere management, strict quality control, customer as god” , get consistent high praise from customers at home and abroad.

Corporate Culture:

Help China Chemicals to benefit the happiness of human life
Become the most trusted chemical supplier in the world
Striver – oriented, enrich employees, customer first, deep service, seek development
Be prepared for danger in times of peace, forge ahead actively, unity and cooperation, and be brave to fight

About Us

The production base is located in Zhangqiu chemical industry park and Tai’an high-tech chemical industry park. laboratory and workshop in strict accordance with the GMP standard and the product fit national ISO9001 and ISO2000 standards.

“Zhishang” products are exported to Europe, North and South America, the Middle East, Asia Pacific and Africa area, so as to establish a long-term and stable cooperation relationship with customer in the world.

Company Info
  • Business Type: Manufacturer
  • Product Range: Cosmetic raw materials
  • Products/Service:Cosmetic raw materials (flavors and fragrances,antioxidant products, moisturizing and hydrating, whitening agents, cosmetic additives)
  • Total Employees: 51~100
  • Capital (Million US $): 10000000RMB
  • Year Established: 2016
Production Capacity
  • No. of Production Lines : 8
  • No. of QC Staff : 5 -10 People
  • OEM Services Provided : yes
  • Factory Size (Sq.meters) : 3,000-5,000 square meters
  • Certificate: ISO9001 , CE , GMP , API , MSDS
  • Factory Location : Diao Town Industry Park, Zhangqiu City, Jinan City, Shandong Province, China.


Pre-Sales Service

* Prompt reply and 24 hours online, professional team to provide best price and high quality product.

* Sample testing support.

* Every batch of products will be tested to ensureits quality.

*The packing also can be according the customers` requirment.

*Any inquiries will be replied within 24 hours.

*we provide Commerical Invoice, Packing List, Bill of loading, COA , Health certificate and Origin certificate. If your markets have any special requirements, let us know.


After-Sales Service

*The fact of logistics information monitoring.

* Any questions about the product can be consulted at any time.

*Product has any problem can return.


Do you accept sample order?

We will make samples before mass production, and after sample approved, we’ll begin mass production. Doing 100% inspection during production, then do random inspection before packing.



You can get free samples for some products,you only need to pay the shipping cost or arrange a courier to us and take the samples. You can send us your product specifications and requests,we will manufacture the products according to your requests.

What’s your MOQ?

Our MOQ is 1kg. But usually we accept less quantity such as 100g on the condition that sample charge is 100% paid.

Do you supply product report?

Yes. We’ll give you product analysis report before shipping.

  Is there a discount?

Different quantity has different discount.


1. ≤50kg, Express delivery recommended, usually called as DDU service;

2. ≤500kg, Air shipping recommended, usually called as FOB, CFR, or CIF service;

3. >500kg, sea shipping recommended, usually called as FOB, CFR, or CIF service;

4. For high value products, please select air shipping and express delivery for safe.

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