L-Alanine, also known as L-α-alanine, is an important natural amino acid and is the most abundant amino acid in the blood. Amino body, with important physiological functions. It is a nutritional supplement, the main component of a variety of compound amino acid infusions, can also be used as a pharmaceutical intermediate, the main raw material of vitamin B6, and can also be used as a food additive to improve the nutritional value of food, improve artificial sweeteners The taste of the flavoring agent.
14.5 º (c=10,6N HCl,dry sub.)
H2O: 100 mg/mL
White to almost white
5.5-6.5 (100g/l, H2O, 20℃)
[α]20/D +14.0±1°, c = 5% in 5 M HCl
166.5 g/L (25 ºC)
L-alanine is made use of as a preservative in food and also beverage, flavor spices and also amino acid low-alcohol wine, etc. In medication, it is used for manufacturing amino acid mixture.
L-alanine can be made use of as a flavor enhancer to increase the flavor result of chemical spices; it can also be utilized as a sour taste corrector to enhance the sour preference of organic acids.
L-alanine is made use of for biochemical research study, and also it is made use of as amino acid nutritional medicine in medicine.
L-Alanine is taken advantage of in nutritional supplements. Flavoring enhancer. For unfermented items, artificial objective, substance sugar, and more, it has recognizable working together impact, and the dose is usually 0.01% to 0.03%. House heating action with sugar (amino-carbonyl reaction) can produce unique aroma materials.
L-Alanine is the raw material for the manufacture of vitamin B6, the synthesis of calcium pantothenate and additionally other natural substances. Including it to food can enhance the flavor impact of chemical spices, enhance the taste of sweeteners in addition to the sour preference of organic acids, boost the premium of alcohols, avoid oil oxidation, as well as enhance the flavor of dipped foods.
L-Alanine improves hydration by balancing the skin’s moisture levels. For this reason, alanine is commonly made use of in cosmetic and also moisturizing products such as foundations, creams, creams, as well as serums.
Preparation of L-Alanine
At present, the preparation methods of L-alanine include enzymatic method and fermentation method. Enzymatic method limits large-scale production due to high energy consumption, high cost, many by-products, low purity, difficult process control, and high environmental pressure. The current production of L-alanine by fermentation has attracted worldwide attention. Compared with the traditional enzymatic method, the fermentation method L-alanine has the advantages of renewable raw material source, mild reaction conditions, good selectivity, less by-products, and low environmental pollution.
CN201810731711.4 provides a method for separating and purifying L-alanine from L-alanine fermentation broth. In the present invention, polyaluminum chloride and sodium polyacrylate are sequentially added to the L-alanine fermentation broth, as shown in Figure 1, the fermentation broth is extracted, and inorganic impurities and biological impurities in the fermentation broth can be fully removed, and the Product purity and high recovery rate can effectively reduce environmental pollution. Include the following steps:
(1) Sterilize the L-alanine fermentation broth prepared by the fermentation method for 0.5h at a temperature of 100℃～120℃;
(2) Add polyaluminum chloride powder to the sterilized fermentation broth at a temperature of 30°C to 60°C, the weight ratio of the fermentation broth to the polyaluminum chloride powder is 100:1 to 2, and fully stir for 10 to 20 minutes. , then add a sodium polyacrylate aqueous solution with a mass concentration of 0.1%, keep the temperature and continue to stir for 20-50min, the addition amount of the sodium polyacrylate aqueous solution with a mass concentration of 0.1% accounts for 1% to 3% of the weight of the fermentation broth, and filter impurities after stirring;
(3) using activated carbon to decolorize the filtered fermentation broth;
(4) the decolorized fermentation broth is distilled under reduced pressure to obtain a concentrated solution, the concentrated solution is allowed to stand at room temperature for 10 to 24 hours and then fully crystallized, and the L-alanine crude product is obtained after filtration;
(5) Dissolving the crude L-alanine product with deionized water to obtain an L-alanine solution with a concentration of 15% to 18%, the L-alanine solution is subjected to a chromatographic column filled with a cation exchange resin. separation and purification, and 50% to 95% ethanol solution is used as the eluent, the working temperature of the chromatographic column is 25 ° C to 70 ° C, and the separated and purified product is dried to obtain L-alanine product with a purity greater than 99%, And the recovery rate of L-alanine product is greater than 75%.
The Purification Method of L-Alanine
200L L-alanine fermentation broth was subjected to ultrafiltration through a ceramic membrane with a molecular weight cut-off of 100,000 Da to obtain L-alanine ultrafiltrate. The specific process conditions are as follows: the ultrafiltration filtration operating pressure is 0.15-0.80 MPa, preferably 0.30-0.60 MPa, and the operating temperature is 10-60°C, preferably 30-60°C. The initial pH of the L-alanine fermentation serum was 6.88, the conductivity was 18643 μS/cm, the L-alanine content was 13.48%, the ammonium salt content was 4029 mg/L, the phosphate content was 2166 mg/L, and the calcium salt content was 1342 mg /L, the magnesium salt content is 1581mg/L.
Take 20L L-alanine ultrafiltrate as desalinated solution and inject it into the fresh water chamber. The initial solution in the concentrated water chamber is 15L 0.05% Na2SO4 solution, and the initial solution in the extremely water chamber is 10L 2% Na2SO4 solution. Start the concentrated water pump, the fresh water pump and the polar water pump, adjust the concentrated and fresh water flow to 1300L/h and the extreme water flow to 1500 L/h, and keep the pressure balance of the concentrated water chamber, the fresh water chamber and the polar water chamber; put the ultrafiltration dialysate in the current Under the condition of density of 120A/m2, electrodialysis treatment until the membrane stack voltage is stable at 35V, and desalination treatment until the conductivity is 3000-4000μS/cm; the membrane stack used in electrodialysis consists of 46 pairs of homogeneous anion exchange membranes and homogeneous cation exchange membranes Composition, the effective membrane area of a single pair of membranes is 0.051m2.
Analysis of the treatment solution obtained by L-alanine electrodialysis: pH was 6.42, conductivity was 3280 μS/cm, which was 17.77% of the initial conductivity, L-alanine content was 12.32%, alanine yield was 91.39%, The content of ammonium salt is 545mg/L, the content of phosphate is 332mg/L, the content of calcium salt is 262mg/L, the content of magnesium salt is 284mg/L, and the desalination rate is 84.39%.
About Products: If you need L-Alanine, a cosmetic raw material, please contact our service staff directly, we will provide you with high-quality products at the best price. As an experienced L-Alanine manufacturer and supplier, ZSpharmac will provide L-Alanine raw materials to customers all over the world for a long time. Over the years, the company has been adhering to the spirit of “integrity management, strict quality control, and customer first”, and has won unanimous praise from domestic and foreign customers.
Company Profile and Corporate Culture
ZhiShang Chemical is owned by ZhiShang Group is a professional new-type chemicals enterprise combined into research and development, production and sales .
The company’s competitive product is pharmaceutical raw materials and intermediates (especially carbohydrate derivatives Series), In recent years, the company has made a major breakthrough in food and feed additives, plant extraction, industrial chemicals industry .
The company insists on the spirit of “sincere management, strict quality control, customer as god” , get consistent high praise from customers at home and abroad.
OUR MISSION Help China Chemicals to benefit the happiness of human life OUR VISSION Become the most trusted chemical supplier in the world OUR BUSINESS PHILOSOPHY Striver – oriented, enrich employees, customer first, deep service, seek development OUR VALUES Be prepared for danger in times of peace, forge ahead actively, unity and cooperation, and be brave to fight
The production base is located in Zhangqiu chemical industry park and Tai’an high-tech chemical industry park. laboratory and workshop in strict accordance with the GMP standard and the product fit national ISO9001 and ISO2000 standards.
“Zhishang” products are exported to Europe, North and South America, the Middle East, Asia Pacific and Africa area, so as to establish a long-term and stable cooperation relationship with customer in the world.
Business Type: Manufacturer
Product Range: Cosmetic raw materials
Products/Service:Cosmetic raw materials (flavors and fragrances,antioxidant products, moisturizing and hydrating, whitening agents, cosmetic additives)
Factory Location : Diao Town Industry Park, Zhangqiu City, Jinan City, Shandong Province, China.
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