Zhishang Chemical

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Zhishang Chemical

Indigo Carmine CAS 860-22-0

Molecular Formula: C16H11N2NaO8S2

Formula Weight: 446.38

ZSpharmac: Indigo Carmine Supplier

Product Name: Indigo Carmine
CAS No: 860-22-0
Purity: 99%

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Basic Info

Product Name:Indigo Carmine
Other Names:Food Blue No. 2
Place of Origin:Shandong, China
Brand Name:ZSpharmac
Type:Cosmetic Raw Materials
Appearance:Very Dark Blue to Purple Powder
EINECS No.:212-728-8
Storage:Room Temp
Provide:Indigo Carmine MSDS;
Indigo Carmine COA

What is Indigo Carmine?

Indigo Carmine is a dark blue powder with a copper metal shine. The liquid solution is blue or blue-purple. 1g is soluble in 100ml of water (25 ° C), somewhat soluble in alcohol, insoluble in a lot of natural solvents. Sensitive to oxidants, the shade will certainly change under the action of nitric acid, as well as the shade of the liquid service will fade after a very long time. It is acquired by sulfonating indigo with oleum. Acid blue 74 can be used as a color, and also can additionally be used to determine kidney feature, test nitrate as well as hydrochloride.

Indigo Carmine Property

Melting point >300°C
density 1.01 g/mL at 20 °C
refractive index n20/D 1.335
storage temp. room temp
solubility 1g/l
form Powder/Solid
Colour Index 73015
color Very dark blue to purple
PH7 (10g/l, H2O, 20℃)
PH Range11.5(blue)-14.0(yellow)
OdorLight odour
Water Solubility 1 g/100 mL (25 ºC)


Indigo Carmine Uses

  1. Indigo Carmine is made use of in the coloring of food, medication and daily cosmetics. Edible indigo is an artificial food coloring. Food coloring is a course of additives utilized for food coloring, including artificial pigments and also all-natural pigments, with a total amount of greater than 60 kinds.
  2. Indigo Carmine is utilized as an oxidant reduction sign and also organic tarnish, and is additionally used for the determination of renal feature.
  3. Indigo Carmine is utilized for food blue coloring. Generally used for tinting. Due to its instability, low fastness and also weak dyeability, it is most suitable for dark tones. Can be readjusted into chocolate color, eco-friendly, red bean shade, tea shade, coffee color. For cakes, cool beverages, rejuvenating drinks, the dosage is 5-100mg/ kg.
  4. Indigo Carmine is utilized as a food coloring agent, which can be made use of in red and also green silk according to my country’s guidelines, and also the maximum use is 0.20 g/kg; it can be made use of in fruit juice (flavored) drinks, carbonated beverages, ready a glass of wine, candies, pastries, and also colored tinned cherries (For design), in environment-friendly plums, the maximum usage quantity is 0.10 g/kg; in dipped side meals, the optimum use amount is 0.01 g/kg.
  5. Indigo Carmine was utilized as a redox indicator. Reagent for screening milk for nitrate and also chlorate. Biological stains, such as the staining of Nicki bodies as well as centers. Determination of renal feature, etc.

Indigo Carmine App

1. Determination of iodine in kelp by Indigo Carmine oxidation fading spectrophotometry. Iodine is known as the “intellectual element” and is one of the essential trace elements for the human body. The iodine content in healthy adults is about 30 mg. Its main function is to promote energy metabolism and heat production in the human body, and promote physical and brain development. Iodine deficiency in early childhood will cause brain development to lag behind in varying degrees, thereby affecting intelligence. , and this effect is irreversible. Excessive eating can also cause goiter and hyperthyroidism. Experts and scholars have found that the IQ of students in high-iodine areas is significantly lower than that in iodine-appropriate areas. Adults should not take more than 2,000 μg of iodine per day, otherwise potentially dangerous goiter will occur. The food sources of iodine are mainly salt, seafood, vegetables and dairy products, etc. It is very meaningful to study and determine trace iodine in kelp.

2 Indigo Carmine Catalytic Fading Spectrophotometric Determination of Cobalt. The use of cobalt to catalyze the oxidative fading reaction of dye-based chromogenic agents has been reported in the determination of trace cobalt in environmental testing, life sciences, food, and medicine. Cobalt was determined using the fading reaction of oxidized acid blue 74. Acid blue 74 (5,5′-indigo sodium sulfonate) reagent, a dye-based chromogenic agent, is a reversible redox reaction system in aqueous solution. In a solution with a pH value < 8.0, its redox potential can be expressed by the following formula :

The pH value of the experimental system is 7.8. Under this condition, the redox pair of the transition metal ion as the catalyst is intermediate between the redox pair of the reactant, that is, XHO-2/OH- > XCo(OH)3/Co( OH )2> XIo /Ir , indicating that Co2+ is the catalyst of this reaction system, and the reaction mechanism is as follows:

The linear range of this method is 0~1.0μg/25 mL, the detection limit is 9.92×10- 7 g/L, and the apparent activation energy of the reaction is 8.66 k J/mol l. Analysis, the result is satisfactory.

3. Determination of protein by Indigo Carmine resonance Rayleigh scattering method. Protein is the material basis of life, and its determination is of great significance in medicine, pharmacy, life science and food nutrition. The photochemical analysis methods for protein determination mainly include absorptiometry and fluorescence analysis. In recent years, Resonance Rayleigh Scattering (RRS), as a new analytical technique, has been widely used in the analysis of biological macromolecules such as proteins and nucleic acids. Based on the aggregation of dye chromophores on biomacromolecules, resulting in strong resonant light scattering.

Studies have shown that the enhancement of RRS signal is related to the molecular weight and electrical properties of proteins. In systems where electrostatic interactions dominate, proteins are positively charged when the pH of the solution is less than the protein’s isopoint. Anionic dyes and proteins combine to form complexes through electrostatic interaction, that is, dyes accumulate on proteins to form larger particles, which generate strong RRS enhancement signals. Using the resonance light scattering technique, the resonance light scattering signal at 393 nm is significantly enhanced through the interaction between protein and acid blue 74 in the medium of pH 2.34, and the enhancement degree is proportional to the protein content. method.

In B-R buffer solution at pH 2.34, the resonance Rayleigh scattering (RRS) of Indigo Carmine and protein are very weak. But when the two are combined, the formed complex can sharply enhance the RRS signal, and the maximum scattering wavelength is 393 nm. The linear ranges of human serum albumin, bovine serum albumin, γ-human globulin and ovalbumin are 0.1~3.7, 0.08~3.0, 0.05~2.0, 0.1~2.4 mg/L respectively; the corresponding detection limits are respectively 24.6, 20.7, 20.4, 25.7 μg/L. This method is used for the determination of total protein in human serum, milk, soybean milk, and urine, and the results are consistent with the classic Coomassie brilliant blue method.

Indigo Carmine's Depigmentation Method

CotA laccase can catalyze the decolorization of Indigo Carmine. The main factors affecting the decolorization of acid blue 74 catalyzed by CotA were temperature, pH and dye mass concentration. Some studies have optimized the decolorization conditions of CotA laccase for the degradation of indigo carmine, and established a ternary quadratic mathematical model. The model fits the relationship between the decolorization rate of Indigo Carmine and each factor well. The three factors of pH, temperature, and dye mass concentration have an interactive effect on the decolorization rate, and the significance of their influence on the decolorization rate is from large to small: pH, temperature, and dye mass concentration. According to the actual situation of the experiment, the optimal degradation conditions were pH = 8.6, temperature 60 ℃, and dye mass concentration 41.5 mg/L. Under these conditions, the decolorization rate of acid blue 74 could reach 91.4%, which was close to the model prediction value. CotA laccase can catalyze the degradation of Indigo Carmine to generate a small molecular compound with a mass-to-charge ratio of 216.07 [M-Na]-, and Indigo Carmine is degraded.

About Products:
If you need Indigo Carmine, the cosmetic raw material, please contact our service staff directly, we will provide you with high-quality products at the best price.
As an experienced Indigo Carmine manufacturer and supplier, ZSpharmac will provide Indigo Carmine raw materials to customers all over the world for a long time.
Over the years, the company has been adhering to the spirit of “integrity management, strict quality control, customer first”, and has won unanimous praise from domestic and foreign customers.

Company Profile and Corporate Culture

Company Profile:

ZhiShang Chemical is owned by ZhiShang Group is a professional new-type chemicals enterprise combined into research and development, production and sales .

The company’s competitive product is pharmaceutical raw materials and intermediates (especially carbohydrate derivatives Series), In recent years, the company has made a major breakthrough in food and feed additives, plant extraction, industrial chemicals industry .

The company insists on the spirit of “sincere management, strict quality control, customer as god” , get consistent high praise from customers at home and abroad.

Corporate Culture:

Help China Chemicals to benefit the happiness of human life
Become the most trusted chemical supplier in the world
Striver – oriented, enrich employees, customer first, deep service, seek development
Be prepared for danger in times of peace, forge ahead actively, unity and cooperation, and be brave to fight

About Us

The production base is located in Zhangqiu chemical industry park and Tai’an high-tech chemical industry park. laboratory and workshop in strict accordance with the GMP standard and the product fit national ISO9001 and ISO2000 standards.

“Zhishang” products are exported to Europe, North and South America, the Middle East, Asia Pacific and Africa area, so as to establish a long-term and stable cooperation relationship with customer in the world.

Company Info
  • Business Type: Manufacturer
  • Product Range: Cosmetic raw materials
  • Products/Service:Cosmetic raw materials (flavors and fragrances,antioxidant products, moisturizing and hydrating, whitening agents, cosmetic additives)
  • Total Employees: 51~100
  • Capital (Million US $): 10000000RMB
  • Year Established: 2016
Production Capacity
  • No. of Production Lines : 8
  • No. of QC Staff : 5 -10 People
  • OEM Services Provided : yes
  • Factory Size (Sq.meters) : 3,000-5,000 square meters
  • Certificate: ISO9001 , CE , GMP , API , MSDS
  • Factory Location : Diao Town Industry Park, Zhangqiu City, Jinan City, Shandong Province, China.


Pre-Sales Service

* Prompt reply and 24 hours online, professional team to provide best price and high quality product.

* Sample testing support.

* Every batch of products will be tested to ensureits quality.

*The packing also can be according the customers` requirment.

*Any inquiries will be replied within 24 hours.

*we provide Commerical Invoice, Packing List, Bill of loading, COA , Health certificate and Origin certificate. If your markets have any special requirements, let us know.


After-Sales Service

*The fact of logistics information monitoring.

* Any questions about the product can be consulted at any time.

*Product has any problem can return.


Do you accept sample order?

We will make samples before mass production, and after sample approved, we’ll begin mass production. Doing 100% inspection during production, then do random inspection before packing.



You can get free samples for some products,you only need to pay the shipping cost or arrange a courier to us and take the samples. You can send us your product specifications and requests,we will manufacture the products according to your requests.

What’s your MOQ?

Our MOQ is 1kg. But usually we accept less quantity such as 100g on the condition that sample charge is 100% paid.

Do you supply product report?

Yes. We’ll give you product analysis report before shipping.

  Is there a discount?

Different quantity has different discount.


1. ≤50kg, Express delivery recommended, usually called as DDU service;

2. ≤500kg, Air shipping recommended, usually called as FOB, CFR, or CIF service;

3. >500kg, sea shipping recommended, usually called as FOB, CFR, or CIF service;

4. For high value products, please select air shipping and express delivery for safe.

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