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Zhishang Chemical

Imperatorin CAS 482-44-0

Molecular Formula: C16H14O4

Formula Weight: 270.28

ZSpharmac: Imperatorin Supplement

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Product Name: Imperatorin
CAS No: 482-44-0
Purity: 99%

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Basic Info

Product Name:Imperatorin
Other Names:Imperatorin, 98%
CAS:482-44-0
Place of Origin:Shandong, China
Brand Name:ZSpharmac
Type:Cosmetic Raw Materials
Appearance:Off-White to Light Brown powder
EINECS No.:207-581-1
Storage:-20°C
Provide:Imperatorin MSDS;
Imperatorin COA

What is Imperatorin?

Imperatorin comes from 6.7-goranocoumarins and also is extensively distributed in the plant kingdom, particularly the frequently utilized conventional Chinese medications of Umbelliferae, such as Angelica dahurica, Duhuo, Qianhu, Qianghuo, Beisha ginseng, and so on are rich and also abundant, and it is the main pharmacological effect. Component.

Imperatorins are plant second metabolites coming from the course of coumarins, especially the furanocoumarins. Imperatorin boosts GABA-induced chloride ion present (IGABA) through α1β2γ2S receptors. Imperatorin enhanced IGABA by 50.5 ± 16.3% at 100 μmol and 109.8 ± 37.7% at 300 μmol. Imperatorin as well as Phellopterin, located in resveratrol root, prevented the binding of diazepam to the benzodiazepine website of rat mind GABAA receptors artificial insemination, with an IC50 of 12.3 μmol for imperatorin and an IC50 was 400 nmol.

Imperatorin, at focus ranging from 3.5 to 14 mmol, significantly and irreversibly inhibits GABA-T in a time- as well as concentration-dependent manner by irreversibly binding to the energetic site of GABA-T. Keratin is a relatively easy to fix acetylcholinesterase (AChE) prevention and also acts in a dose-dependent way. The pain and also BChE inhibitory tasks of imperatorin as well as unrefined removes from Angelica fruit were evaluated spectrophotometrically at concentrations of 12.5, 25, 50 as well as 100 μg/ mL. The inhibition rate of imperatorin on pains was low (13.75-46.11%), while the repressive effect on BChE was significant (37.46-83.98%). Imperatorin is selective for BChE over AChE with an IC50 of 31.4 μmol for BChE. Imperatorin together with (+)-Byakangelicol was discovered to be one of the most powerful BACE-1 prevention with IC50 of 91.8 as well as 104.9 μmol, respectively.

Imperatorin Properties:

Melting point 98-100°C
Boiling point 333.4°C (rough estimate)
density 1.1311 (rough estimate)
refractive index 1.4350 (estimate)
storage temp. -20°C
solubility DMSO: ≥5mg/mL
form powder
color off-white to light brown

 

Imperatorin Uses

  1. Imperatorin is used for content determination/identification/pharmacological experiments, etc. Pharmacological effects: Imperatorin has antibacterial, antiasthmatic and antiallergic effects.

Applications of Imperatorin

Study on the effect of imperatorin on the process of liver fibrosis in the regulation of HNF-4α
Thioacetamide (TAA) was used to induce liver fibrosis in mice, to explore the effect of imperatorin on HNF-4α on the process of liver fibrosis, and to provide certain insights for imperatorin to become a potential drug for liver fibrosis. Theoretical basis.

Methods: (1) C57BL/6 mice were selected for in vivo experiments and randomly divided into six groups: normal group, model group, IM low-dose administration group (20 mg/kg), IM high-dose administration group (50 mg/kg) ), curcumin positive control group (20 mg/kg), IM single administration group (50 mg/kg). Liver fibrosis model was established by intraperitoneal injection of TAA (100 mg/kg or 200 mg/kg) for 5 weeks. Serum was collected to detect the level of liver transaminase in mice; H&E staining and Sirius red staining were used to observe the pathological changes of liver; western blot and RT-PCR were used to detect liver fibrosis indicators (α-SMA, Collagen-I, TIMP-1, MMP) -13), inflammatory cytokines (F4/80, MPO, caspase-1, IL1R1, IL-1β); Western blot, RT-PCR, immunofluorescence and immunohistochemistry were used to detect HNF-4α in total protein, plasma protein and regulation in nucleoproteins.

(2) In vitro experiments, rat hepatic stellate cells (HSCs) were activated with TGF-β (10 ng/mL) for 1 h, and different concentrations of IM (3.125, 6.25, 12.5 μM) were administered and incubated for 6 hours. h, Western blot and RT-PCR were used to detect the protein and mRNA expression levels of HNF-4α, liver fibrosis indicators and inflammatory cytokines; as well as the different expressions of HNF-4α in nucleoprotein and plasma protein.

(3) The si HNF-4α experiment was performed on human hepatic stellate cells (LX-2), and the liver fibrosis indicators (α-SMA, Collagen-I, TIMP-1, MMP) were detected under the condition of reduced HNF-4α expression. -13) and the expression changes of inflammatory cytokines (caspase-1, IL1R1, IL-1β). Results: In the experiment of liver fibrosis induced by TAA, the levels of ALT and AST in serum increased, and IM administration could reduce the levels of ALT and AST. H&E staining showed that IM alleviated the pathological damage of mouse liver tissue.

Sirius red staining showed that collagen deposition in mice was improved after IM administration. Western blot and RT-PCR results showed that IM significantly inhibited the expression levels of liver fibrosis indicators α-SMA, Collagen-I, TIMP-1, MMP-13, decreased MPO, F4/80, IL1R1, caspase-1, IL The release of inflammatory cytokines such as -1β reduces liver inflammation. In addition, IM can up-regulate the expression of HNF-4α in total and plasma proteins, inhibit its expression in the nucleus, and improve liver fibrosis through the activation of HNF-4α.

In cell experiments, IM can reduce the protein and mRNA levels of α-SMA, Collagen-I, and TIMP-1 in activated HSCs, down-regulate the ratio of TIMP-1/MMP-13, and inhibit IL1R1, caspase-1, IL- The release of inflammatory cytokines such as 1β. And IM can inhibit the activation of hepatic stellate cells by up-regulating the expression level of HNF-4α in total cell protein and plasma protein. After silencing HNF-4α siRNA, the expression of HNF-4α decreased, and HNF-4α was activated after IM administration. However, the inhibitory effect of IM on inflammatory cytokines and liver fibrosis markers was weakened under the condition of HNF-4α inhibition.

Conclusion: Imperatorin reduces ECM deposition and inhibits activation of hepatic stellate cells by up-regulating the expression of HNF-4α; imperatorin inhibits the release of inflammatory cytokines such as F4/80, IL1R1, and caspase-1, and reduces hepatic inflammatory response. Imperatorin ameliorated TAA-induced liver fibrosis by activating the expression of HNF-4α in the liver, reducing hepatic collagen deposition and secretion of inflammatory cytokines.

Extraction Method of Imperatorin

A method for extracting imperatorin, characterized in that the method comprises the following steps:

1) Alcohol extraction: take the coarse powder of Cnidium chinensis, extract 2-6 times with 3-8 times the amount of 10-90% ethanol, combine medicinal liquids, filter, recover ethanol under reduced pressure and concentrate to extract, which is divided into oil layer and Water layer, take the oil layer;

2) Extraction of crystallization: the oil layer is extracted with petroleum ether and left to stand to separate out crystals, filter, and after the mother liquor is placed to solidify, ethanol is added to heat to dissolve, and crystals are precipitated after being placed, and recrystallized with absolute ethanol to obtain the imperatorin product.

The specific implementation method is:

Take 100 g of Cnidium chinensis coarse powder, soak it with 5 times the amount of 80% ethanol for 3 times, combine the medicinal liquid, filter, recover the ethanol under reduced pressure, and concentrate it to an extract shape, which is divided into an oil layer and a water layer. Set, separate out crystallization, filter, after the mother liquor is placed and solidified, add ethanol heating and dissolving, separate out crystallization after placing, with absolute ethanol recrystallization, obtain 3.69g pale yellow bulk crystal-imperatorin, HPLC detection purity is 99.3 %.

Research on Imperatorin

Wang Mengyue et al. used the hot plate method and the acetic acid writhing method to study the analgesia of the four main components of the Radix Angelica sinensis, including imperatorin, isoimperatorin, hydrated oxidized imperatorin, and white angelica. Activity. It was found that these four coumarins can significantly reduce the number of writhing in mice induced by acetic acid. Imperatorin and iso-imperatorin can also significantly delay the appearance of writhing in mice; and imperatorin And iso-imperatorin have significant analgesic effect, can obviously inhibit the pain of mice caused by hot plate method, the effect of imperatorin is obviously stronger than isoimperatorin.

M.J.Abad et al. believed that imperatorin was a dual inhibitor of cyclooxygenase and lipoxygenase, so it could be developed as a new type of anti-inflammatory agent. H.S.Ba et al. found that imperatorin could inhibit the prostatic activity of rat peritoneal macrophages It also inhibits the production of cyclooxygenase-2 and prostaglandin E synthases (mPEEs). It has anti-vaccine effect against a variety of bacteria, such as Escherichia coli, Shigella solani, Shigella freundii, Proteus, Typhoid Bacillus, Paratyphoid Bacillus, Pseudomonas aeruginosa, Bacillus cholera, Gram-positive bacteria, human Mycobacterium tuberculosis, Staphylococcus aureus, etc. have different degrees of inhibition. In addition, imperatorin family has significant antiviral activity. E.S. Rosselli et al. found that imperatorin has strong inhibitory activity against both apple orchid-positive bacteria and gram-negative bacteria (minimum inhibitory concentration 32-128mg.ml). Y.S.Kwon et al. studied the antibacterial components in Angelica dahurica root, and obtained a new component, 5,8-bis(2,3-bis(2,3-dihydroxy-3-methylbutanilide) psoralen, which is similar to ferulic acid Six coumarin components such as acid and iso-imperatorin have antibacterial effects. In addition, imperatorin can also inhibit the pseudotype of vesicular stomatitis virus or the human immunodeficiency virus type 1 (HIV -1 Infection of T cells and HeLa cells.

Imperatorin and isoimperatorin are isomers and have certain similarities in pharmacological effects. However, the efficacy of the drug is different; it has a certain inhibitory effect on drug metabolizing enzymes. Therefore, when combined with other drugs, attention should be paid to the interaction between drugs to achieve the purpose of rational drug use. At the same time, with the deepening of research and the improvement of pharmacokinetics, it is of great significance for the rational clinical application of traditional Chinese medicines and Chinese patent medicines with imperatorin water and isoimperatorin as the main components.

Company Profile and Corporate Culture

Company Profile:

ZhiShang Chemical is owned by ZhiShang Group is a professional new-type chemicals enterprise combined into research and development, production and sales .

The company’s competitive product is pharmaceutical raw materials and intermediates (especially carbohydrate derivatives Series), In recent years, the company has made a major breakthrough in food and feed additives, plant extraction, industrial chemicals industry .

The company insists on the spirit of “sincere management, strict quality control, customer as god” , get consistent high praise from customers at home and abroad.

Corporate Culture:

OUR MISSION
Help China Chemicals to benefit the happiness of human life
OUR VISSION
Become the most trusted chemical supplier in the world
OUR BUSINESS PHILOSOPHY
Striver – oriented, enrich employees, customer first, deep service, seek development
OUR VALUES
Be prepared for danger in times of peace, forge ahead actively, unity and cooperation, and be brave to fight

About Us

The production base is located in Zhangqiu chemical industry park and Tai’an high-tech chemical industry park. laboratory and workshop in strict accordance with the GMP standard and the product fit national ISO9001 and ISO2000 standards.

“Zhishang” products are exported to Europe, North and South America, the Middle East, Asia Pacific and Africa area, so as to establish a long-term and stable cooperation relationship with customer in the world.

Company Info
  • Business Type: Manufacturer
  • Product Range: Additive , Chemical Auxiliary & Catalyst , Organic Chemicals
  • Products/Service: Organic Intermediate,Inorganic Chemistry, APIs, Dyestuffs And Pigments, Fragrance And Spices, Food Additives
  • Total Employees: 51~100
  • Capital (Million US $): 10000000RMB
  • Year Established: 2016
Production Capacity
  • No. of Production Lines : 8
  • No. of QC Staff : 5 -10 People
  • OEM Services Provided : yes
  • Factory Size (Sq.meters) : 3,000-5,000 square meters
  • Certificate: ISO9001 , CE , GMP , API , MSDS
  • Factory Location : Diao Town Industry Park, Zhangqiu City, Jinan City, Shandong Province, China.

Service

Pre-Sales Service

* Prompt reply and 24 hours online, professional team to provide best price and high quality product.

* Sample testing support.

* Every batch of products will be tested to ensureits quality.

*The packing also can be according the customers` requirment.

*Any inquiries will be replied within 24 hours.

*we provide Commerical Invoice, Packing List, Bill of loading, COA , Health certificate and Origin certificate. If your markets have any special requirements, let us know.

 

After-Sales Service

*The fact of logistics information monitoring.

* Any questions about the product can be consulted at any time.

*Product has any problem can return.

FAQ

Do you accept sample order?

We will make samples before mass production, and after sample approved, we’ll begin mass production. Doing 100% inspection during production, then do random inspection before packing.

 

HOW TO CONFIRM THE PRODUCT QUALITY BEFORE PLACING ORDERS?

You can get free samples for some products,you only need to pay the shipping cost or arrange a courier to us and take the samples. You can send us your product specifications and requests,we will manufacture the products according to your requests.

What’s your MOQ?

Our MOQ is 1kg. But usually we accept less quantity such as 100g on the condition that sample charge is 100% paid.

Do you supply product report?

Yes. We’ll give you product analysis report before shipping.

  Is there a discount?

Different quantity has different discount.

Shipping

1. ≤50kg, Express delivery recommended, usually called as DDU service;

2. ≤500kg, Air shipping recommended, usually called as FOB, CFR, or CIF service;

3. >500kg, sea shipping recommended, usually called as FOB, CFR, or CIF service;

4. For high value products, please select air shipping and express delivery for safe.

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