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Imperatorin CAS 482-44-0

Molecular Formula: C16H14O4

Formula Weight: 270.28

ZSpharmac: Imperatorin Supplier

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Product Name: Imperatorin
CAS No: 482-44-0
Purity: 99%

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Basic Info

Product Name:Imperatorin
Other Names:Imperatorin, 98%
CAS:482-44-0
Place of Origin:Shandong, China
Brand Name:ZSpharmac
Type:Cosmetic Raw Materials
Appearance:Off-White to Light Brown Powder
EINECS No.:207-581-1
Storage:-20°C
Provide:Imperatorin MSDS;
Imperatorin COA

What is Imperatorin?

Imperatorin and iso-impemtorin belong to 6.7-thynocoumarins, which are widely distributed in the plant kingdom, especially the traditional Chinese medicines commonly used in Umbelliferae such as Angelica dahurica, Duhuo, Qianhu, Notopterygium, North Radix Ginseng etc. contain and are abundant, and it is its main pharmacological effect component.

Imperatorin Property

Melting point 98-100°C
Boiling point 333.4°C (rough estimate)
density 1.1311 (rough estimate)
refractive index 1.4350 (estimate)
storage temp. -20°C
solubility DMSO: ≥5mg/mL
form powder
color off-white to light brown

 

Application of Imperatorin

It is used in the study of the influence of imperatorin on HNF-4α regulating the process of liver fibrosis
Using Thioacetamide (Thioacetamide, TAA) to induce liver fibrosis in mice, explore the effect of imperatorin on HNF-4α on the process of liver fibrosis, and provide certain evidence for imperatorin as a potential drug for the treatment of liver fibrosis Theoretical basis.

Methods: (1) C57BL/6 mice were selected for in vivo experiments and randomly divided into six groups: normal group, model group, IM low-dose administration group (20 mg/kg), IM high-dose administration group (50 mg/kg ), curcumin positive control group (20 mg/kg), IM single administration group (50 mg/kg). Liver fibrosis model was established by intraperitoneal injection of TAA (100 mg/kg or 200 mg/kg) for 5 weeks. Serum was collected to detect liver transaminase levels in mice; liver histopathological changes were observed by H&E staining and Sirius red staining; liver fibrosis indicators (α-SMA, Collagen-I, TIMP-1, MMP -13), inflammatory cytokines (F4/80, MPO, caspase-1, IL1R1, IL-1β); use western blot, RT-PCR, immunofluorescence and immunohistochemistry to detect HNF-4α in total protein, plasma protein and regulatory roles in nucleoproteins.

(2) For in vitro experiments, rat hepatic stellate cells (HSCs) were activated with TGF-β (10 ng/m L) for 1 h, and incubated with different concentrations of IM (3.125, 6.25, 12.5 μM) for 6 h, Western blot and RT-PCR were used to detect the protein and mRNA expression levels of HNF-4α, liver fibrosis indicators and inflammatory cytokines; and the different expression of HNF-4α in nucleoprotein and plasma protein.

(3) The siHNF-4α experiment was performed on human hepatic stellate cells (LX-2). Under the condition that the expression of HNF-4α was reduced, the indicators of liver fibrosis (α-SMA, Collagen-I, TIMP-1, MMP -13) and the expression changes of inflammatory cytokines (caspase-1, IL1R1, IL-1β). Results: In TAA-induced liver fibrosis experiments, the levels of ALT and AST in serum increased, and the levels of ALT and AST could be reduced after IM administration. H&E staining showed that IM alleviated the pathological damage of mouse liver tissue.

Sirius red staining showed that collagen deposition in mice was improved after IM administration. The results of Western blot and RT-PCR showed that IM significantly inhibited the expression levels of liver fibrosis indicators α-SMA, Collagen-I, TIMP-1, and MMP-13, and decreased the expression levels of MPO, F4/80, IL1R1, caspase-1, and IL The release of inflammatory cytokines such as -1β can reduce liver inflammation. In addition, IM can up-regulate the expression of HNF-4α in total protein and plasma protein, inhibit its expression in nucleus, and improve liver fibrosis through the activation of HNF-4α.

In cell experiments, IM can reduce the protein and mRNA levels of α-SMA, Collagen-I, and TIMP-1 in activated HSCs, down-regulate the ratio of TIMP-1/MMP-13, and inhibit IL1R1, caspase-1, and IL-1. Release of inflammatory cytokines such as 1β. And IM can inhibit the activation of hepatic stellate cells by up-regulating the expression level of HNF-4α in total cell protein and plasma protein. After silencing HNF-4α siRNA, the expression of HNF-4α decreased, HNF-4α was activated after IM administration, and the activation of HNF-4α inhibited the expression of α-SMA, Collagen-I, caspase-1, IL-1β, etc. However, the inhibitory effect of IM on inflammatory cytokines and liver fibrosis markers was weakened under the condition of HNF-4α inhibition.

Conclusion: Imeratorin reduces ECM deposition and inhibits the activation of hepatic stellate cells by up-regulating the expression of HNF-4α; imperatorin inhibits the release of inflammatory cytokines such as F4/80, IL1R1, caspase-1, and reduces liver inflammatory response. Imimperatorin improved TAA-induced liver fibrosis by activating the expression of HNF-4α in the liver, reducing hepatic collagen deposition and secretion of inflammatory cytokines.

Extraction Method of Imperatorin

A method for extracting imperatorin, characterized in that said method comprises the following steps:

1) Alcohol extraction: Take the coarse powder of Fructus Cnidii, extract 2-6 times with 3-8 times the amount of 10-90% ethanol, combine the liquid medicine, filter, recover the ethanol under reduced pressure and concentrate to extract, and divide into oil layer and Water layer, take oil layer;

2) Extraction and crystallization: the oil layer is extracted with petroleum ether and left to stand, crystals are precipitated, filtered, and the mother liquor is left to solidify, then ethanol is added to heat and dissolve, and crystals are precipitated after standing, and recrystallized with absolute ethanol to obtain the imperatorin product.

The specific implementation method is:

Take 100g of cnidium seed powder, soak it with 5 times the amount of 80% ethanol for 3 times, combine the medicinal solution, filter, recover the ethanol under reduced pressure and concentrate it into an extract, and divide it into an oil layer and a water layer. The oil layer is extracted with petroleum ether and then statically Place, crystallize out, filter, after the mother liquor is placed and solidified, add ethanol to heat and dissolve, and place to precipitate crystals, recrystallize with absolute ethanol to obtain 3.69g light yellow block crystals-imperatorin, and the HPLC detection purity is 99.3 %.

Related Research on Imperatorin

Wang Mengyue et al used the hot plate method, acetic acid twisting method, etc. to study the analgesic effects of the four main linear components of imperatorin, isoimperatorin, hydrated oxidized imperatorin, and white angelica. activity. It was found that these four coumarins could significantly reduce the number of writhing in mice induced by acetic acid. Imimperatorin and isoimperatorin could also significantly delay the appearance of writhing in mice; and imperatorin Both imperatorin and isoimperatorin have significant analgesic effects, and can significantly inhibit the pain of mice caused by the hot plate method, and the effect of imperatorin is significantly stronger than that of isoimperatorin.

M.J.Abad et al. believed that imperatorin is a dual inhibitor of cyclooxygenase and lipoxygenase, so it can be developed into a new type of anti-inflammatory agent. H.S.Ba et al. found that imperatorin can inhibit prostate cancer in rat peritoneal macrophages It can also inhibit the production of cyclooxygenase-2 and prostaglandin E synthase (mPEEs). It has an anti-vaccine effect and is effective against a variety of bacteria, such as Escherichia coli, Shigella sonnei, Shigella flexneri, Proteus, typhoid bacillus, paratyphoid bacillus, Pseudomonas aeruginosa, cholerae bacillus, Gram-positive bacteria, human Type Mycobacterium tuberculosis, Staphylococcus aureus, etc. have varying degrees of inhibition. In addition, imperatorin has significant antiviral activity E.S.Rosselli and other studies found that imperatorin has strong inhibitory activity against apple-positive bacteria and Gram-negative bacteria (minimum inhibitory concentration 32-128mg.ml). Y.S.Kwon et al. have studied the antibacterial components in the root of Angelica dahurica, and obtained a new component of 5,8-bis(2,3-dihydrogen-3-methylbutadrinyl psoralen). The 6 kinds of coumarin components such as acid and isoimperatorin all have antibacterial effects. In addition, imperatorin can also inhibit the human immunodeficiency virus type 1 (HIV -1 Infection of T cells and HeLa cells.

Imimperatorin and isoimperatorin are isomers, and have certain similarities in pharmacological effects. However, the strength of the drug is different; it has a certain inhibitory effect on drug-metabolizing enzymes. Therefore, when it is used in combination with other drugs, attention should be paid to the interaction between drugs in order to achieve the purpose of rational drug use. At the same time, with the deepening of research and the improvement of pharmacokinetics, it is of great significance for the rational clinical application of traditional Chinese medicine and Chinese patent medicine with imperatorin water and isoimperatorin as the main components.

About Products:
If you need Imperatorin as a cosmetic raw material, please contact our service staff directly, we will provide you with high-quality products at the best price.
As an experienced Imperatorin manufacturer and supplier, ZSpharmac will provide Imperatorin raw materials to customers all over the world for a long time.
Over the years, the company has been adhering to the spirit of “integrity management, strict quality control, customer first”, and has won unanimous praise from domestic and foreign customers.

Company Profile and Corporate Culture

Company Profile:

ZhiShang Chemical is owned by ZhiShang Group is a professional new-type chemicals enterprise combined into research and development, production and sales .

The company’s competitive product is pharmaceutical raw materials and intermediates (especially carbohydrate derivatives Series), In recent years, the company has made a major breakthrough in food and feed additives, plant extraction, industrial chemicals industry .

The company insists on the spirit of “sincere management, strict quality control, customer as god” , get consistent high praise from customers at home and abroad.

Corporate Culture:

OUR MISSION
Help China Chemicals to benefit the happiness of human life
OUR VISSION
Become the most trusted chemical supplier in the world
OUR BUSINESS PHILOSOPHY
Striver – oriented, enrich employees, customer first, deep service, seek development
OUR VALUES
Be prepared for danger in times of peace, forge ahead actively, unity and cooperation, and be brave to fight

About Us

The production base is located in Zhangqiu chemical industry park and Tai’an high-tech chemical industry park. laboratory and workshop in strict accordance with the GMP standard and the product fit national ISO9001 and ISO2000 standards.

“Zhishang” products are exported to Europe, North and South America, the Middle East, Asia Pacific and Africa area, so as to establish a long-term and stable cooperation relationship with customer in the world.

Company Info
  • Business Type: Manufacturer
  • Product Range: Cosmetic raw materials
  • Products/Service:Cosmetic raw materials (flavors and fragrances,antioxidant products, moisturizing and hydrating, whitening agents, cosmetic additives)
  • Total Employees: 51~100
  • Capital (Million US $): 10000000RMB
  • Year Established: 2016
Production Capacity
  • No. of Production Lines : 8
  • No. of QC Staff : 5 -10 People
  • OEM Services Provided : yes
  • Factory Size (Sq.meters) : 3,000-5,000 square meters
  • Certificate: ISO9001 , CE , GMP , API , MSDS
  • Factory Location : Diao Town Industry Park, Zhangqiu City, Jinan City, Shandong Province, China.

Service

Pre-Sales Service

* Prompt reply and 24 hours online, professional team to provide best price and high quality product.

* Sample testing support.

* Every batch of products will be tested to ensureits quality.

*The packing also can be according the customers` requirment.

*Any inquiries will be replied within 24 hours.

*we provide Commerical Invoice, Packing List, Bill of loading, COA , Health certificate and Origin certificate. If your markets have any special requirements, let us know.

 

After-Sales Service

*The fact of logistics information monitoring.

* Any questions about the product can be consulted at any time.

*Product has any problem can return.

FAQ

Do you accept sample order?

We will make samples before mass production, and after sample approved, we’ll begin mass production. Doing 100% inspection during production, then do random inspection before packing.

 

HOW TO CONFIRM THE PRODUCT QUALITY BEFORE PLACING ORDERS?

You can get free samples for some products,you only need to pay the shipping cost or arrange a courier to us and take the samples. You can send us your product specifications and requests,we will manufacture the products according to your requests.

What’s your MOQ?

Our MOQ is 1kg. But usually we accept less quantity such as 100g on the condition that sample charge is 100% paid.

Do you supply product report?

Yes. We’ll give you product analysis report before shipping.

  Is there a discount?

Different quantity has different discount.

Shipping

1. ≤50kg, Express delivery recommended, usually called as DDU service;

2. ≤500kg, Air shipping recommended, usually called as FOB, CFR, or CIF service;

3. >500kg, sea shipping recommended, usually called as FOB, CFR, or CIF service;

4. For high value products, please select air shipping and express delivery for safe.

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