Icariin Powder CAS 489-32-7

1. Thin layer identification method
Take 0.5 g of epimedium medicinal powder or an ideal amount of extract, include 10ml of ethanol, take in warm temperature for half an hour, filter, vaporize the filtrate to dryness, include 1ml of ethanol to the residue to dissolve, which is the examination remedy. Weigh the icariin referral substance, dissolve it in methanol and dilute it to 0.1 mg/ml, which is the recommendation material remedy. Take 10 μl each of the test remedy and the recommendation solution, and put them on the very same silica gel H thin-layer plate with salt carboxymethylcellulose as the adhesive, and utilize ethyl acetate-butanone-formic acid-water (10:1: 1:1) is the creating agent, unravel it, take it out, completely dry it, and examine it under a UV lamp (365nm). In the chromatogram of the examination item, the same dark red places show up at the settings corresponding to the chromatogram of the recommendation material; spray with trichloride Aluminum examination option, and then placed under UV light (365nm) for inspection, the exact same orange-red fluorescent areas were disclosed.
2. Decision of complete flavonoid material in Epimedium and its extracts by spectrophotometry
Precisely evaluate the reference substance icariin and prepare a typical service of 10ug/ml with methanol. Precisely weigh a suitable amount of the powder of this item (equivalent to around 1 mg of icariin), ultrasonicate with 25 ml of methanol for thirty minutes, weaken to volume in a 50 ml gauging bottle, and filter with a 0.45 μm microporous membrane layer to prepare for screening. product solution. Take the examination option and the recommendation solution respectively, make use of the reagent as the blank, gauge the absorbance at a wavelength of 270nm, and determine it.
3. HPLC approach to identify the content of icariin in Epimedium and its essences
Chromatographic conditions and system suitability examination: ODS25uC18 (4mm × 150mm) chromatographic column; mobile stage: acetonitrile-water (30:70); discovery wavelength: 270nm; flow price: 0.8 ml/min. The variety of academic plates based upon icariin is not less than 1500.
Preparation of the reference substance solution: Exactly consider the recommendation material icariin and prepare a 0.1 mg/ml remedy with methanol.
Preparation of the examination option: Precisely evaluate an ideal quantity of this item powder (comparable to around 1 mg of icariin), sonicate with 25 ml of methanol for half an hour, water down to quantity in a 50 ml determining container, filter with a 0.45 μm micropore Membrane layer filtering is the test service.
Decision method: Inject 10 μl of each example examination remedy and recommendation service right into the HPLC instrument, and compute the location utilizing the external criterion approach.