Hydrolyzed Animal Protein CAS 92113-31-0

A rapid method for establishing the molecular weight of polypeptides in Hydrolyzed Animal Protein oral fluid, consisting of the complying with steps:
( 1) Prepare the examination example: Take 10ml of readily available hydrolyzed collagen oral solution. The mass focus of Hydrolyzed Animal Healthy Protein is 105g/L. Include 200μL of deionized water and also mix equally. Then add 5ml of 0.1 mol/L Tris-HCL buffer., 2 ml of 2,5-dihydroxybenzoic acid was mixed and centrifuged, and then the filter membrane was utilized for the initial rugged purification to acquire the filtrate;

( 2) Pass the obtained filtrate through a special gel chromatography column, and also pass it via the gel chromatography column under the very same conditions as a standard with a known molecular weight to compute the molecular weight of the sample to be checked. The computation formula is: Ve=k-klogMr, where Ve is the elution quantity, k and also k are constant coefficients, and also Mr is the molecular weight.

Amongst them, the continuous coefficients k and k are computed with the elution quantity and molecular weight of the typical substance, and afterwards incorporated with the elution volume of the example to be tested, they are applied to the formula to calculate its molecular weight; the Tris-HCL barrier likewise includes mass LHAC with a focus of 0.02 mol/L; pepsin can be contributed to the filtrate in (2) prior to passing through an unique gel chromatography column. The amount of enzyme added is 3% of the filtrate. Stir equally at 5 ° C and also let stand. Leave it for 8 hrs, suspend the enzyme and after that centrifuge for 30 minutes. Take the supernatant to further detoxify the collagen peptides in the dental remedy as well as enhance the precision of the examination results; the requirement is bovine serum albumin; the special coagulant The gel chromatography column is made by blending cross-linked dextran, bio-glucan-P and bagasse at a mass ratio of 1:2:0.015 and after that swelling it in no seawater for 20 hrs. Initially, stir continuously for 2 hrs with a mixing rod to totally blend the three., and then stir intermittently every two hours. The stirring pole is a warmed mixing pole, which can speed up the swelling of the gel. After it is totally puffy, it can be packed right into a column after vacuum cleaner filtering as well as degassing; the bagasse has been treated with ultrafiberization, which can enhance The pore stability and also distribution harmony of the gel can additionally ensure that it has enough mechanical stamina.