1. Stone sulfur method
Take 1 kg of honeysuckle, add water to decoct twice, 2 hours each time, combine the decoction liquid, filter, add lime milk to the filtrate to adjust the pH value to 10-12, let it stand, filter out the precipitate, add appropriate amount of water, and adjust the pH value to 6-6 with sulfuric acid 7. Stir well, filter, concentrate the filtrate to a thick paste, and spray dry to obtain the honeysuckle extract.
2. Improved stone sulfur method
Take 1kg of honeysuckle, add (15, 10) times the amount of water, extract twice at 80-90°C for 2 hours each time, filter, combine the filtrate, concentrate the filtrate to 2/5 of the original volume, add 20% milk of lime Adjust the pH value to 10-12, let it stand for 12 hours, filter out the precipitate, add 2 times the amount of 95% ethanol to suspend, add 50% sulfuric acid to adjust the pH value to 3-4, mix well, filter, and adjust the pH value of the filtrate with 40% NaOH 6-7, filter, concentrate to a thick paste, and spray dry to obtain the honeysuckle extract.
3. Ultrafiltration treatment
Take 1 kg of honeysuckle, add (15, 10) times the amount of water, decoct twice, 2 hours each time, filter, combine the filtrate, concentrate the filtrate to 2/5 of the original volume, let it stand, filter, ultra-filter, The ultrafiltrate was collected, concentrated to the crude drug amount, allowed to stand, filtered, concentrated under reduced pressure, and vacuum-dried to obtain the honeysuckle extract.
4. Ethanol reflux extraction
Weigh 1 kg of honeysuckle, put it in a round bottom flask, add (10, 8) times the amount of 70% ethanol, heat and reflux for extraction twice, each time for 2 hours, filter, combine the filtrates, concentrate under reduced pressure, and vacuum dry to obtain the honeysuckle extract.
5. Macroporous adsorption resin treatment
Take 1 kg of honeysuckle, add (15, 10) times the amount of water, extract twice at 90°C for 2 hours each time, filter, combine the filtrate, add the filtrate to the pretreated macroporous adsorption resin, add water to elute, collect and wash The liquid was removed, concentrated under reduced pressure, and dried in vacuum to obtain the honeysuckle extract.