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Hesperetin CAS 520-33-2

Molecular Formula: C16H14O6

Formula Weight: 302.28

ZSpharmac: Hesperetin Supplier

Product Name: Hesperetin
CAS No: 520-33-2
Purity: 99%

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Basic Info

Product Name:Hesperetin
Other Names:Hesperetin, 98%
Place of Origin:Shandong, China
Brand Name:ZSpharmac
Type:Cosmetic Raw Materials
Appearance:White to Pale Yellow Solid
EINECS No.:208-290-2
Storage:Inert Atmosphere,Room Temperature
Provide:Hesperetin MSDS;
Hesperetin COA

What is Hesperetin?

Hesperetin (Hesperetin) is a kind of natural flavonoids, widely present in fruits, flowers, foods and other plant-derived substances. It is mainly derived from the hydrolysis of hesperidin (Hesperidin), has biological and pharmacological activities, and is widely used in many fields such as organic chemistry, medicine, agronomy, and food.

Hesperetin Property

Melting point 230-232°C
Boiling point 363.32°C (rough estimate)
density 1.2514 (rough estimate)
refractive index -4 ° (C=1.8, EtOH)
storage temp. Inert atmosphere,Room Temperature
solubility DMSO (Slightly), Methanol (Slightly)
form Solid
color White to Pale Yellow
Water Solubility Soluble in water (partly), dilute alkalis, and ethanol (50 mg/ml).


Pharmacological Action of Hesperetin

1. Results on central nerves associated conditions
Parkinson’s illness (PD) is a neurodegenerative condition caused by the loss of dopaminergic afferent neuron in the brain. PD eventually leads to electric motor as well as cognitive impairment in individuals. The protective result of hesperetin on striatal 6-hydroxydopamine-injured rats was evaluated, and its possible system of action in apoptosis, swelling and also oxidative anxiety was explored.

2. Hypoglycemic impact
Diabetes mellitus is a team of metabolic diseases that has become a significant public health trouble, enhancing the social and also economic worry. Hyperglycemia and hyperlipidemia prevail features of diabetic issues, leading to alterations in sugar as well as lipid metabolism, and degrees of liver enzymes. Persistent hyperglycemia, defined by consistent and abnormally high postprandial blood sugar levels, has actually become a key factor in the beginning of diabetes mellitus. Rat research studies have actually shown that hesperetin can efficiently protect the normal histological morphology of STZ-induced rat liver, kidney as well as insulin-positive β-cells.

3. Antitoxin hemagglutinin effect
Poisonous snakebites are an international, neglected public health trouble. The World Health Company reported in 2017 that concerning 5.5 million individuals are attacked by venomous snakes yearly, about 400,000 individuals pass away from specific kinds of sequelae, such as amputation, as well as 20,000 to 125,000 individuals die from deadly injuries. Two snake venom serine proteases 1 and 2 (SVSP1, SVSP2) were isolated from the venom of Simes rotus by gel filtration as well as reverse chromatography (RP-HPLC), as well as their loved one molecular masses were established to be concerning 31300 and also 24600. Sequencing of SVSP1 and SVSP2 using tandem mass spectrometry (Q-TOF) as well as in silico docking using a serine protease structure (PDB access: 4e7n) known to be homologous to the two target proteins revealed that hesperetin is the Protein-targeted preventions. The kinetic criteria of SVSPs were acquired with in vitro experiments on hesperetin. The results showed that hesperetin may be a non-competitive inhibitor of SVSP1 and also SVSP2, and it can be made use of as an inexpensive prevention for the research study of snake venom proteases.

4. Anti-fibrosis effect
Cardiac makeover is a significant determinant of heart failure (cardiac arrest), identified by cardiac hypertrophy, fibrosis, oxidative tension, as well as myocyte apoptosis. Utilizing the computer mouse design of cardiac arrest induced by thoracic aortic banding (AB), through the monitoring of heart mass/body mass, lung mass/body mass, heart mass/tibia length, echocardiogram as well as hemodynamic criteria, as well as histology As well as data such as gene expression of hypertrophic and fibrotic markers were assessed, it was discovered that hesperetin avoided AB-induced cardiac hypertrophy, fibrosis as well as cardiac disorder in computer mice, and also attenuated oxidative tension and also cardiomyocyte apoptosis. Further studies found that hesperetin hindered heart improvement by obstructing PKCα/ βII-Akt, JNK and TGF-β1-Smad signaling paths. The study found that a brand-new hesperetin derivative 7 [8-( N-methylpiperazinyl) hesperetin] can inhibit the induction of platelet-derived development element BB (PDGF-BB) via the Wnt/ β-catenin pathway. Activation and also expansion of hepatic stellate cells.

5. Anti-tumor effect
In human lung cancer H522 cells, hesperetin caused a cell signaling pathway independent of the p53 path, as well as hesperetin up-regulated the expression of Fas, Fas-associated death domain name healthy protein (FADD) as well as caspase-8 proteins in H522 cells levels, and decreased the expression degrees of caspase-3, caspase-9, p53 and Bax healthy proteins. The results showed that hesperetin not only stimulated apoptosis through the path of p53 and Bax, however additionally set off the FADD/caspase -8 dependent apoptosis path composed of fatality receptors. To study the result of hesperetin on 7,12-dimethylphenylpropanthracene (DMBA)-induced buccal pouch squamous cell cancer in hamsters, via the results of lipid peroxidation (LPO) byproducts, enzyme standing, non-enzyme resistance The examination of oxidant, antidote as well as various other elements assessed its chemopreventive effect, and the results revealed that hesperetin could prevent DMBA-induced hamster squamous cell cancer.

Preparation of Hesperetin

A method for extracting high-purity hesperetin from Fructus Fructus Aurantii, is characterized in that, the method comprises the following steps:

Step 1, after crushing Fructus Fructus Fructus Fructus Aurantii, use alcohol solvent reflux to extract three times, the amount of alcohol solvent used for the first reflux extraction is 5 to 20 times of the quality of Fructus Fructus Fructus Fructus Fructus, the time of reflux extraction is 1 hour to 2 hours, and the amount of alcohol solvent extracted by reflux for the second time The amount of alcohol solvent is 5-20 times of the quality of Citrus aurantium, and the reflux extraction time is 1 hour to 2 hours. After filtering, the filtrates were combined three times, and the combined filtrates were concentrated under reduced pressure to a concentrated solution with a specific gravity of 1.0 to 1.06;

Step 2. Add mineral acid to the concentrated solution in step 1 to obtain a mixed solution, hydrolyze the mixed solution at a temperature of 90°C to 120°C for 3 hours to 10 hours, naturally cool to room temperature, and filter to obtain The filter residue with a temperature of 80°C to 100°C is washed with soft water and then centrifuged to remove the supernatant, repeated washing and centrifugation for 1 to 3 times to obtain crude hesperetin; the mass concentration of the inorganic acid in the mixed solution is 3% to 5% %;

Step 3, dissolving the hesperetin crude product described in step 2 with ethanol with a mass concentration of more than 85%, adding activated carbon to the dissolved solution for reflux decolorization for 1 hour to 2 hours, and then decolorizing the solution at a temperature of 50 Filtrate under the condition of ℃~80℃, concentrate the filtered filtrate to 1/4 of the volume of the filtrate, then place the concentrated filtrate at room temperature to crystallize and then filter, dry the filtered solid matter to obtain a mass purity of not less than 96% hesperetin crystalline.

Industrial Production Method of Hesperetin

(1) Water phase technique GrohmannK et al. made use of the water stage as the reaction tool, dilute sulfuric acid as the catalyst, and also the reaction temperature level varied from 25 ° C to 180 ° C. The study located that when the temperature in the water stage was 120 ° C or greater, orange peel Glycoside hydrolysis price was significantly accelerated. This is because the solubility of hesperidin in aqueous medium is very little at space temperature level, and enhancing the reaction temperature can significantly increase the solubility of hesperidin in liquid phase. Hesperidin in the aqueous stage was mostly hydrolyzed to produce hesperetin-7-β-D-glucoside, as well as part of hesperetin-7-β-D-glucoside could be more hydrolyzed to produce hesperetin (Number 1). Hesperetin-7-β-D-glucoside is also an important natural intermediate, which can be utilized in the synthesis of sweetener (dihydrochalcone).

( 2) Methanol technique Thomas Seitz et al. chose methanol as the medium for the hydrolysis of hesperidin, focused sulfuric acid as the stimulant, warmed and also refluxed for 7.5 hrs, as well as the reaction ended. After including ethyl acetate option, washing with water, drying as well as concentrating, the crude item was gotten. The crude item was dissolved in acetone, added dropwise to water down acetic acid remedy, filtered, focused and also dried to get pure item (99% return). The methanol approach has a basic synthesis procedure as well as the highest product return, but the splitting up as well as filtration of the item is troublesome as well as calls for toxic methanol as a response tool, so it does not meet the manufacturing requirements of environment-friendly environmental management. Wingard et alia improved the methanol method reported by Thomas Seitz. When the hydrolysis response is over, first of all get rid of the excess methanol under decreased pressure, and after that directly add the response option to the water stage under the protection of nitrogen, warmth and reflux for 2 hrs, trendy, and also an unrefined product is precipitated (yield 85%), and the unrefined product can be washed with water. Hesperetin with a purity of 97% can be acquired.

About Products:
If you need Hesperetin, a cosmetic raw material, please contact our service staff directly, and we will provide you with high-quality products at the best price.
As an experienced manufacturer and supplier of Hesperetin, ZSpharmac will provide Hesperetin raw materials to customers all over the world for a long time.
Over the years, the company has been adhering to the spirit of “integrity management, strict quality control, customer first”, and has won unanimous praise from domestic and foreign customers.

Company Profile and Corporate Culture

Company Profile:

ZhiShang Chemical is owned by ZhiShang Group is a professional new-type chemicals enterprise combined into research and development, production and sales .

The company’s competitive product is pharmaceutical raw materials and intermediates (especially carbohydrate derivatives Series), In recent years, the company has made a major breakthrough in food and feed additives, plant extraction, industrial chemicals industry .

The company insists on the spirit of “sincere management, strict quality control, customer as god” , get consistent high praise from customers at home and abroad.

Corporate Culture:

Help China Chemicals to benefit the happiness of human life
Become the most trusted chemical supplier in the world
Striver – oriented, enrich employees, customer first, deep service, seek development
Be prepared for danger in times of peace, forge ahead actively, unity and cooperation, and be brave to fight

About Us

The production base is located in Zhangqiu chemical industry park and Tai’an high-tech chemical industry park. laboratory and workshop in strict accordance with the GMP standard and the product fit national ISO9001 and ISO2000 standards.

“Zhishang” products are exported to Europe, North and South America, the Middle East, Asia Pacific and Africa area, so as to establish a long-term and stable cooperation relationship with customer in the world.

Company Info
  • Business Type: Manufacturer
  • Product Range: Cosmetic raw materials
  • Products/Service:Cosmetic raw materials (flavors and fragrances,antioxidant products, moisturizing and hydrating, whitening agents, cosmetic additives)
  • Total Employees: 51~100
  • Capital (Million US $): 10000000RMB
  • Year Established: 2016
Production Capacity
  • No. of Production Lines : 8
  • No. of QC Staff : 5 -10 People
  • OEM Services Provided : yes
  • Factory Size (Sq.meters) : 3,000-5,000 square meters
  • Certificate: ISO9001 , CE , GMP , API , MSDS
  • Factory Location : Diao Town Industry Park, Zhangqiu City, Jinan City, Shandong Province, China.


Pre-Sales Service

* Prompt reply and 24 hours online, professional team to provide best price and high quality product.

* Sample testing support.

* Every batch of products will be tested to ensureits quality.

*The packing also can be according the customers` requirment.

*Any inquiries will be replied within 24 hours.

*we provide Commerical Invoice, Packing List, Bill of loading, COA , Health certificate and Origin certificate. If your markets have any special requirements, let us know.


After-Sales Service

*The fact of logistics information monitoring.

* Any questions about the product can be consulted at any time.

*Product has any problem can return.


Do you accept sample order?

We will make samples before mass production, and after sample approved, we’ll begin mass production. Doing 100% inspection during production, then do random inspection before packing.



You can get free samples for some products,you only need to pay the shipping cost or arrange a courier to us and take the samples. You can send us your product specifications and requests,we will manufacture the products according to your requests.

What’s your MOQ?

Our MOQ is 1kg. But usually we accept less quantity such as 100g on the condition that sample charge is 100% paid.

Do you supply product report?

Yes. We’ll give you product analysis report before shipping.

  Is there a discount?

Different quantity has different discount.


1. ≤50kg, Express delivery recommended, usually called as DDU service;

2. ≤500kg, Air shipping recommended, usually called as FOB, CFR, or CIF service;

3. >500kg, sea shipping recommended, usually called as FOB, CFR, or CIF service;

4. For high value products, please select air shipping and express delivery for safe.

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