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Hematoxylin CAS 517-28-2

Molecular Formula: C16H14O6

Formula Weight: 302.28

ZSpharmac: Hematoxylin Raw Material Supplier

Product Name: Hematoxylin
CAS No: 517-28-2
Purity: 99%

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Basic Info

Product Name:Hematoxylin
Place of Origin:Shandong, China
Brand Name:ZSpharmac
Type:Cosmetic Raw Materials
Appearance:Yellow to Brown Powder
EINECS No.:208-237-3
Storage:Room Temperature
Provide:Hematoxylin MSDS;
Hematoxylin COA

What is Hematoxylin?

Hematoxylin, also known as hematoxylin, is a natural phenolic compound extracted from Haematoxylon campechianum or other similar plants. It usually exists in the form of trihydrate and has a chemical formula of C16H14O6. It is a dark blue to purple crystal that is widely used in cytology and other biological fields, especially as an important stain.

Hematoxylin itself is insoluble in water, but soluble in alcohol, ether and alkaline solutions. In the process of cell staining, hematoxylin is often used to stain cell nuclei and certain cell components because it can bind to acidic substances in cells, such as nucleic acids (DNA and RNA), thus making the nucleus appear dark blue to blue purple. In the classic hematoxylin and eosin (H&E staining) staining method, hematoxylin is used to stain the nucleus, while eosin is used to stain the cytoplasm and other intracytoplasmic components.

Hematoxylin Property

Melting point 200 °C (dec.)(lit.)
Boiling point 363.32°C (rough estimate)
density 1.2514 (rough estimate)
vapor pressure 0-0Pa at 20-25℃
refractive index 1.4600 (estimate)
storage temp. Keep in dark place,Inert atmosphere,Room temperature
solubility Soluble in 95% ethanol(1 mg/mL).
Colour Index 75290
pka6.7(at 25℃)
form Powder/Solid
color Yellow to Brown
PH RangeRed (0.0) to yellow (1.0);Pale yellow (5.0) to violet (6.0)


Hematoxylin Uses

Biomedical and Life Sciences:
Cytological and histological staining: Hematoxylin is one of the most commonly used dyes, primarily used to stain cell nuclei in tissue sections. In the classic hematoxylin and eosin (H&E) staining method, hematoxylin is responsible for staining the nucleus, showing dark blue, while eosin stains the cytoplasm and other structures, thereby revealing the details of the internal structure of the cell. It is widely used Pathological diagnosis and scientific research work.
Cell biology research: Hematoxylin is also used in the pre-staining step of fluorescent staining. For example, hematoxylin staining is performed before fluorescent antibody staining to subsequently observe the position and morphology of the cell nucleus.

Scientific research reagents:
Biochemical experiments: Hematoxylin can form complexes with certain metal ions, so under specific conditions, it can be used as an indicator or determination reagent for metal ions.
Genetic research: In DNA and RNA staining, hematoxylin is sometimes used for preliminary localization or rough observation of nucleic acid distribution due to its affinity with nucleic acids.

Other areas:
Ink Manufacturing: Hematoxylin has been used in the past to make blue ink, but this use is less common due to the advent of modern synthetic dyes.
Industrial uses: Derivatives of hematoxylin or related compounds may have a role in certain industrial applications, such as textile dyeing, wood preservation, or the paper industry, but these uses are relatively rare and usually require specific chemical modifications.

Application of Hematoxylin in Cosmetics

Extraction of natural ingredients: Hematoxylin is extracted from natural plants. Today, the cosmetics industry is increasingly focusing on the use of ingredients from natural sources. Theoretically, if sufficient safety and effectiveness evaluation is carried out, the extract or its derivatives may be used in certain Used as natural pigments or additional beneficial ingredients in some cosmetics.

Antioxidant: Because hematoxylin contains phenolic hydroxyl groups, which are similar to certain phenolic compounds with antioxidant properties, it has potential antioxidant effects.

Hair dye: Hematoxylin has dyeing ability, and some literature mentions it as a natural dye, but this mainly refers to some hematoxylin extracts that are not chemically modified and can be dyed directly under certain conditions.

Advantages of Hematoxylin

  1. Efficient dyeing performance:
    Hematoxylin is an excellent nuclear dye that has a high affinity for nucleic acids (especially DNA) and can effectively stain cell nuclei into dark blue. This property is particularly prominent in histological and cytological research. The nucleus and cytoplasm can be clearly distinguished by Hematoxylin-Eosin (H&E) staining.
  2. Moderate stability:
    Although hematoxylin itself is unstable, its stability in solution can be significantly improved after appropriate oxidation and metal ion treatment (such as forming hematoxylin-iron complex with iron ions), making it easier to store and use for a long time.
  3. Natural sources:
    Hematoxylin is mainly extracted from plants (such as hematoxylin plants). It has a certain degree of naturalness and environmental protection, and meets the needs of some industries for green and sustainable chemical raw materials.
  4. Versatility:
    Under certain conditions, hematoxylin can form complexes with other metal ions, so it can be used as a chromogen or indicator for metal ions in some experiments and detections.
  5. safety:
    As a traditional dye with a long history, hematoxylin is relatively less irritating to human skin and tissue under appropriate concentrations and conditions of use. It has been widely used in the fields of medicine and scientific research, indicating that it has a certain degree of safety.


Langenbacher RE, Horoszko CP, Kim M, Heller DA. Hematoxylin Nuclear Stain Reports Oxidative Stress via Near-Infrared Emission. ACS Chem Biol. 2023 May 19;18(5):1237-1245. doi: 10.1021/acschembio.3c00156. Epub 2023 Apr 18. PMID: 37070948; PMCID: PMC10289833.

As a company specializing in the production and supply of Hematoxylin raw materials, we are committed to providing customers with high-quality products and preferential prices. We have rich experience and technology to ensure product quality and stable supply. No matter where you are, we can provide you with the Hematoxylin raw materials you need to support your production and research and development.

We always adhere to the principle of honest operation, strictly control product quality, and serve every customer with a customer-first attitude. Over the years, we have established good cooperative relationships with customers at home and abroad and won their trust and praise.

If you have needs or are interested in our products, please contact our service staff directly, we will wholeheartedly provide you with the best quality services and products. Looking forward to cooperating with you and developing together!

Company Profile and Corporate Culture

Company Profile:

ZhiShang Chemical is owned by ZhiShang Group is a professional new-type chemicals enterprise combined into research and development, production and sales .

The company’s competitive product is pharmaceutical raw materials and intermediates (especially carbohydrate derivatives Series), In recent years, the company has made a major breakthrough in food and feed additives, plant extraction, industrial chemicals industry .

The company insists on the spirit of “sincere management, strict quality control, customer as god” , get consistent high praise from customers at home and abroad.

Corporate Culture:

Help China Chemicals to benefit the happiness of human life
Become the most trusted chemical supplier in the world
Striver – oriented, enrich employees, customer first, deep service, seek development
Be prepared for danger in times of peace, forge ahead actively, unity and cooperation, and be brave to fight

About Us

The production base is located in Zhangqiu chemical industry park and Tai’an high-tech chemical industry park. laboratory and workshop in strict accordance with the GMP standard and the product fit national ISO9001 and ISO2000 standards.

“Zhishang” products are exported to Europe, North and South America, the Middle East, Asia Pacific and Africa area, so as to establish a long-term and stable cooperation relationship with customer in the world.

Company Info
  • Business Type: Manufacturer
  • Product Range: Cosmetic raw materials
  • Products/Service:Cosmetic raw materials (flavors and fragrances,antioxidant products, moisturizing and hydrating, whitening agents, cosmetic additives)
  • Total Employees: 51~100
  • Capital (Million US $): 10000000RMB
  • Year Established: 2016
Production Capacity
  • No. of Production Lines : 8
  • No. of QC Staff : 5 -10 People
  • OEM Services Provided : yes
  • Factory Size (Sq.meters) : 3,000-5,000 square meters
  • Certificate: ISO9001 , CE , GMP , API , MSDS
  • Factory Location : Diao Town Industry Park, Zhangqiu City, Jinan City, Shandong Province, China.


Pre-Sales Service

* Prompt reply and 24 hours online, professional team to provide best price and high quality product.

* Sample testing support.

* Every batch of products will be tested to ensureits quality.

*The packing also can be according the customers` requirment.

*Any inquiries will be replied within 24 hours.

*we provide Commerical Invoice, Packing List, Bill of loading, COA , Health certificate and Origin certificate. If your markets have any special requirements, let us know.


After-Sales Service

*The fact of logistics information monitoring.

* Any questions about the product can be consulted at any time.

*Product has any problem can return.


Do you accept sample order?

We will make samples before mass production, and after sample approved, we’ll begin mass production. Doing 100% inspection during production, then do random inspection before packing.



You can get free samples for some products,you only need to pay the shipping cost or arrange a courier to us and take the samples. You can send us your product specifications and requests,we will manufacture the products according to your requests.

What’s your MOQ?

Our MOQ is 1kg. But usually we accept less quantity such as 100g on the condition that sample charge is 100% paid.

Do you supply product report?

Yes. We’ll give you product analysis report before shipping.

  Is there a discount?

Different quantity has different discount.


1. ≤50kg, Express delivery recommended, usually called as DDU service;

2. ≤500kg, Air shipping recommended, usually called as FOB, CFR, or CIF service;

3. >500kg, sea shipping recommended, usually called as FOB, CFR, or CIF service;

4. For high value products, please select air shipping and express delivery for safe.

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