The preparation techniques of Hematoporphyrin consist of base matter-organic solvent approach, antarctic acetic acid technique, acetone approach, methanol or methanol-ethanol technique, ion exchange cellulose approach
Technique 1. Basic substance-organic solvent approach.
Include 60L of methanol as well as 1kg of diethylamine right into the response storage tank, mix evenly, include 5kg of fresh bovine blood powder, mix as well as extract at 45 ° C for 1 hour, cool, filter, concentrate the filtrate to 5L, include 10L of antarctic acetic acid, 200g of strontium chloride, and warmth as well as boil down Get rid of recurring methanol, warm up to 100-102 ° C for 1 h, cool, filter, and also accumulate hemin crystals. Hematoporphyrin crystals were cleaned together with glacial acetic acid, water and acetone, and dried out to get 85 g of Hematoporphyrin.
Bovine blood dish [methanol, diethylamine] → [45 ℃, 1h] [filter] → filtrate [ice Hac, SrCl2] → [100-102 ℃,1 h] [filter, laundry, completely dry] → hemin.
Take 60L of methanol and also 5kg of spray-dried bovine erythrocytes into the response tank, mix evenly, essence at 40 ° C for half an hour, trendy, filter, concentrate the filtrate to 5L, include 10L of antarctic acetic acid, 100g of sodium chloride, add 2L of water to dissolve, heat and distill Get rid of recurring methanol, warm up to 100 ° C for 1 h, cool, filter, as well as collect Hematoporphyrin crystals. The hemin crystals were washed successively with glacial acetic acid as well as water, and dried out to acquire 65 g of hemin.
Bovine erythrocytes [methanol] → [40 ℃, 30min] [filter] → filtrate [ice Hac, NaCl, H2O] → [100 ℃,1 h] [filter, clean, completely dry] → hemin.
Prep work of heme Take 300kg of fresh blood meal, include methanol 1200L, add 30kg of dichloroacetic acid, stir well, stir and remove at 45 ° C for 1h, cool, filter, and focus the filtrate to dryness to obtain 45kg of Hematoporphyrin.
New blood meal [methanol, dichloroacetic acid] → [45 ° C, 1h] [filteringed system, concentrated] → heme.
Preparation of Hematoporphyrin Take 45kg of Hematoporphyrin, add 100L of glacial acetic acid having 2kg of NaCl, boil and also reflux for extraction for 1 hr, cool and also filter to obtain hemin, clean with glacial acetic acid and also water in turn, and dry to get 4.8 kg of hemin.
Heme [blended solvent of NaCl and also antarctic acetic acid] → [steaming for 1h] [washing as well as drying] → hemin.
Method 2, methanol-ethanol mixed solvent removal.
Preparation of acidic blood dish: Take 100 kg of blood dish, include 100 kg of 0.6 mol/L HCl option, mix for hemolysis, and spray completely dry to get 40 kg of acidic blood dish.
Blood dish [HCl] → acidic blood dish.
Preparation of Hematoporphyrin Take 40kg of acidic blood dish, remove 3 times with a mixed solvent made up of 90% ethanol, 6% methanol and also 4% water, the combined solvent dose is 800L, 300L as well as 200L respectively, integrate the supernatant, distill as well as concentrate to prepare Hematoporphyrin. Acid blood dish [mixed solvent] → [essence 3 times] supernatant [concentrate] → Hematoporphyrin.