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Guanidine Thiocyanate CAS 593-84-0

Molecular Formula: CH5N3.CHNS

Formula Weight: 118.16

ZSpharmac: Guanidine Thiocyanate Supplier

Product Name: Guanidine Thiocyanate
CAS No: 593-84-0
Purity: 99%

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Basic Info

Product Name:Guanidine Thiocyanate
Other Names:GITC
Place of Origin:Shandong, China
Brand Name:ZSpharmac
Type:Cosmetic Raw Materials
Appearance:White or Colorless Crystals Powder
EINECS No.:209-812-1
Provide:Guanidine Thiocyanate MSDS;
Guanidine Thiocyanate COA

What is Guanidine Thiocyanate?

Guanidine isothiocyanate remains in the form of white crystals with a melting point of 116-121 ° C. At 20 ° C, the pH value of 6mol/L in water is 4.5-6, and also the solubility is totally dissolved. It is a type of uncoupling agent, which is a sort of solid protein denaturant, which can liquify healthy protein, make the secondary framework of protein vanish, deteriorate cell structure, as well as swiftly separate nucleoprotein from nucleic acid. For denaturing as well as lysing cells; drawing out rna and dna, no rnase and also dnase activity.

Guanidine Thiocyanate Property

Melting point 120-122 °C(lit.)
density 1.103 g/mL at 20 °C
vapor pressure 0Pa at 20℃
refractive index n20/D 1.482
storage temp. 20-25°C
solubility H2O: 6 M at 20 °C, clear, colorless
form Crystals or Crystalline Powder
pka12.5[at 20 ℃]
color White or colorless
Specific Gravity1.152
PH4.8-6.0 (1420g/l, H2O, 20℃)(saturated solution)
PH Range4.8 – 6.0
Water Solubility Soluble in water and ethanol


Application of Guanidine Isothiocyanate in the Field of Chemical Depilatories

Chemical depilatory is a kind of depilatory that can make the hair soft and also the strength of the hair lower, and also the hair can be wiped or rinsed within 2 to 6 minutes. Considering that the structure of the hair shaft resembles that of the skin, chemical depilatory agents will unavoidably harm the skin. Consequently, depilatory agents are noted as special-purpose cosmetics.
Thioglycolic acid depilatory representative is the major depilatory representative nowadays, it progressively replaces sulfide depilatory representative. The depilatory actives used in such depilatory representatives are thioglycolic acid and its salts, mercaptopropionic acid and its salts, mercaptolactic acid and its salts. Thinking about efficiency as well as price, thioglycolic acid and also its salts are mostly utilized as depilatory active compounds.
The application of some swelling representatives (Swellingagents) aids to quicken the depilatory effect of the mercapto substance depilatory agent, thus lowering the inflammation to the skin. Such swelling representatives consist of melamine, dicyandiamide or a mixture of both (w=0.5% to 2%), thiourea, potassium thiocyanate, guanidine thiocyanate and also PVP copolymer.

Application Examples of Guanidine Thiocyanate

Application one,
Hou Xiaofeng from the Department of Biochemistry of PLA General Hospital conducted methodological evaluation and performance verification on the detection of lactate dehydrogenase isoenzyme 1 (LDH1) by guanidine thiocyanate inhibition method. Methods: The imprecision, linear analysis, recovery experiment and normal reference range of LDH1 detection by guanidine thiocyanate inhibition method were evaluated. Results: The intra-assay imprecision of high, medium and low value samples were 4.25%, 1.57% and 0.97%, respectively, and the inter-assay imprecision were 4.57%, 2.55% and 1.20%, respectively; the dilution robustness of the linear analysis was good, R2 =0.998; the average recovery rate was 101.56%; there was no significant difference in LDH1 level between genders; the normal reference range was 33.11±5.76U/L. Conclusion: The guanidinium thiocyanate inhibition method for detecting LDH1 is reliable, has good performance, meets the laboratory requirements, and can be widely used in clinical practice.

Application two,
CN201110146840.5 discloses a method for preparing total RNA without genomic DNA, using sodium dodecyl sulfate as a lysis reagent, and using potassium acetate and sodium dodecyl sulfate to react to generate potassium dodecyl sulfate (PDS) after lysing cells Precipitates, co-precipitates proteins as well as most of the genomic DNA. Then, utilizing the property that nucleic acid can be adsorbed by the phenol extract when guanidinium thiocyanate exists, and the adsorbed nucleic acid can be dissociated by a certain concentration of potassium acetate according to the molecular weight, a certain concentration of guanidinium thiocyanate and acidic acetic acid are added to the nucleic acid. Potassium, total RNA and residual genomic DNA were isolated by phenol extraction. Finally, total RNA samples were obtained by ethanol precipitation. Compared with the prior art, this method has the least residual genomic DNA in the obtained RNA sample, which is 103 copies per microgram (about 4 picograms).

Application three.
CN201711222569.2 provides a lysate for extracting nucleic acid from exfoliated cells in human feces, including: 1mol/L~8mol/L guanidinium salt, 1mM~20mM metal ion chelating agent and 100mM-Tris-HCl buffer solution with pH7.5~8.0, Wherein, the guanidine salt is selected from at least one of guanidine hydrochloride, guanidine isothiocyanate and guanidine thiocyanate, and the metal ion chelating agent is selected from at least one of ethylenediaminetetraacetic acid and water-soluble salts of ethylenediaminetetraacetic acid. kind. Preferably, it also includes 1% volume to 20% volume of surfactant, the surfactant is a non-ionic detergent, and the non-ionic detergent is selected from Tween-20, Tween-80, Brij-35, NP40 and At least one of Triton-100. Related preparation methods and applications are also provided. The lysate of the present invention can rapidly and efficiently lyse cells, fully release nucleic acid, meet the amount of subsequent nucleic acid detection, and is suitable for large-scale popularization and application.

The Preparation Method in Guanidinium Thiocyanate

report one
A kind of preparation method of guanidinium thiocyanate, has following technological process: a, put guanidinium carbonate and ammonium thiocyanate that weight ratio is 2: 1 into reactor, react at 50~90 ℃ for 2 hours, get thiocyanate Guanidine acid crude product. B, dissolving, at normal temperature, the guanidine thiocyanate crude product is dissolved in water in a ratio of 1:1; c, filtering, removing impurities such as raw materials and reaction by-products by filtering; d, dehydrating, filtering the The mother liquor is dehydrated at high temperature; e, crystallization, the supersaturated solution is cooled, concentrated and crystallized, and high-purity guanidine thiocyanate is obtained.

report two,
A kind of preparation method of guanidinium thiocyanate, it is characterized in that having following technical process: (1) put into reaction kettle that the weight ratio is 2: guanidine carbonate and ammonium sulfate of 1, react 1.5 hours at 45-95 ℃, The crude guanidine thiocyanate was obtained. (2) dissolving, at normal temperature, the guanidine thiocyanate crude product is dissolved in water in the ratio of 0.5: 1; (3) filtering, removing impurities such as raw materials and reaction by-products by filtering; (4) dehydrating, filtering The final mother liquor is dehydrated at high temperature; (5) crystallization, the solution is cooled, concentrated and crystallized, and high-purity guanidine thiocyanate is obtained.

About Products:
If you need Guanidine thiocyanate as a cosmetic raw material, please contact our service staff directly, we will provide you with high-quality products at the best price.
As an experienced Guanidine thiocyanate manufacturer and supplier, ZSpharmac will provide Guanidine thiocyanate raw materials for customers all over the world for a long time.
Over the years, the company has been adhering to the spirit of “integrity management, strict quality control, customer first”, and has won unanimous praise from domestic and foreign customers.

Company Profile and Corporate Culture

Company Profile:

ZhiShang Chemical is owned by ZhiShang Group is a professional new-type chemicals enterprise combined into research and development, production and sales .

The company’s competitive product is pharmaceutical raw materials and intermediates (especially carbohydrate derivatives Series), In recent years, the company has made a major breakthrough in food and feed additives, plant extraction, industrial chemicals industry .

The company insists on the spirit of “sincere management, strict quality control, customer as god” , get consistent high praise from customers at home and abroad.

Corporate Culture:

Help China Chemicals to benefit the happiness of human life
Become the most trusted chemical supplier in the world
Striver – oriented, enrich employees, customer first, deep service, seek development
Be prepared for danger in times of peace, forge ahead actively, unity and cooperation, and be brave to fight

About Us

The production base is located in Zhangqiu chemical industry park and Tai’an high-tech chemical industry park. laboratory and workshop in strict accordance with the GMP standard and the product fit national ISO9001 and ISO2000 standards.

“Zhishang” products are exported to Europe, North and South America, the Middle East, Asia Pacific and Africa area, so as to establish a long-term and stable cooperation relationship with customer in the world.

Company Info
  • Business Type: Manufacturer
  • Product Range: Cosmetic raw materials
  • Products/Service:Cosmetic raw materials (flavors and fragrances,antioxidant products, moisturizing and hydrating, whitening agents, cosmetic additives)
  • Total Employees: 51~100
  • Capital (Million US $): 10000000RMB
  • Year Established: 2016
Production Capacity
  • No. of Production Lines : 8
  • No. of QC Staff : 5 -10 People
  • OEM Services Provided : yes
  • Factory Size (Sq.meters) : 3,000-5,000 square meters
  • Certificate: ISO9001 , CE , GMP , API , MSDS
  • Factory Location : Diao Town Industry Park, Zhangqiu City, Jinan City, Shandong Province, China.


Pre-Sales Service

* Prompt reply and 24 hours online, professional team to provide best price and high quality product.

* Sample testing support.

* Every batch of products will be tested to ensureits quality.

*The packing also can be according the customers` requirment.

*Any inquiries will be replied within 24 hours.

*we provide Commerical Invoice, Packing List, Bill of loading, COA , Health certificate and Origin certificate. If your markets have any special requirements, let us know.


After-Sales Service

*The fact of logistics information monitoring.

* Any questions about the product can be consulted at any time.

*Product has any problem can return.


Do you accept sample order?

We will make samples before mass production, and after sample approved, we’ll begin mass production. Doing 100% inspection during production, then do random inspection before packing.



You can get free samples for some products,you only need to pay the shipping cost or arrange a courier to us and take the samples. You can send us your product specifications and requests,we will manufacture the products according to your requests.

What’s your MOQ?

Our MOQ is 1kg. But usually we accept less quantity such as 100g on the condition that sample charge is 100% paid.

Do you supply product report?

Yes. We’ll give you product analysis report before shipping.

  Is there a discount?

Different quantity has different discount.


1. ≤50kg, Express delivery recommended, usually called as DDU service;

2. ≤500kg, Air shipping recommended, usually called as FOB, CFR, or CIF service;

3. >500kg, sea shipping recommended, usually called as FOB, CFR, or CIF service;

4. For high value products, please select air shipping and express delivery for safe.

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