A kind of production method of high-purity Glutathion is characterized in that the method comprises the following steps:
(1) centrifugation of the fermented liquid: centrifuging after the glutathione fermented liquid that the fermentation level reaches at least 1650mg/L is put into the tank, the centrifugal speed is 3000~5000rpm, and the time is 5~10min to obtain the wet yeast cell body;
(2) hot water wall breaking: 1 part of wet yeast cell body is added in the extraction tank of 6~10 parts of boiling water, stir, stirring speed is 300~350rpm, when extracting liquid temperature reaches 85~95 ℃ After being incubated for 10 to 15 minutes, stop the extraction, pour out the extract, and cool the extract to room temperature in a water bath;
(3) Centrifugation of the extract: the centrifugation of the extract, the centrifugal speed is 3000~5000rpm, and the time is 5~10min, the yeast residue is removed, and the extract containing GSH is obtained;
(4) adjust pH: add acid in the extract after centrifugation, make feed liquid system be in the acidic environment of pH 2.5~3.5, can precipitate some impurity, and make GSH be in steady state, prevent oxidation ;
(5) Microfiltration: Utilize the microfiltration equipment of 0.1~1 μ m aperture to filter the extract after pH adjustment, to remove suspended small particle impurities, obtain clear and transparent filtrate;
(6) Cationic resin separation: the feed liquid after microfiltration is adsorbed on the sulfonic acid group cationic resin in an acidic environment, GSH is adsorbed, and then analyzed with sodium chloride or hydrochloric acid solution to obtain a liquid rich in GSH;
(7) Adsorption resin separation: the GSH-rich liquid separated and purified by cation exchange resin is adsorbed again on styrene-based weakly polar macroporous adsorption resin, and then eluted with pure water to obtain GSH with high purity liquid;
(8) concentrating under reduced pressure: under the vacuum degree of-0.07~-0.098Mpa and 55~65 DEG C of temperature, concentrate the GSH solution under reduced pressure, make to reach GSH content more than 400mg/ml;
(9) Crystallization: add isopropanol or ethanol to the Glutathion concentrated solution to make the organic solvent content reach 60%~80%, adjust the pH value to 2.90~3.0, and carry out crystallization at 0~4°C environment;
(10) crystal washing: the crystals are washed with the acetone solution of 70% to 90% by volume percent concentration;
(11) Vacuum drying: dry under the vacuum degree of-0.07~-0.098Mpa, obtain the GSH of more than 98% purity.