Genistein Supplement CAS 529-59-9

Genistein Supplement compounds include a variety of members with highly diverse chemical structures and biological functions, such as glycitein, mangiferin, daidzin, and genistin. The main ingredient of soy isoflavones extracted from soybeans is Genistein Supplement. Genistin is one of the active ingredients of soybean isoflavones, which belongs to phytoestrogens structurally. Studies have found that genistein cannot be directly absorbed by the body, but is absorbed in the form of genistein after being hydrolyzed by enzymes in intestinal bacteria to exert biological activity. Genistein is more active than daidzein in many aspects.

CN201711287750.1 provides a method for detecting genistin in honey vinegar black beans. The method adopts methanol ultrasonic-assisted extraction method to extract genistin in honey vinegar black beans, and uses ultra-high performance liquid chromatography to detect its content, through the preparation of honey vinegar black soybean extract, standard solution preparation, and sample solution preparation and ultra-high performance liquid chromatography detection steps to obtain the content of genistin in honey vinegar black beans. Include the following specific steps:

S1. Preparation of honey vinegar black soybean extract: crush the dried honey vinegar black soybeans, sieve to obtain honey vinegar black soybean powder, add methanol solution A for ultrasonication, take the supernatant after centrifugation, concentrate the supernatant, and use methanol Solution B was made to a constant volume to obtain a genistin extract;

S2. Preparation of genistein standard solution: the genistein standard product was constant volume with methanol solution C, and ultrasonically treated to make Genistein Supplement standard mother liquor, and the genistein standard mother liquor was constant volume with methanol solution C to make genistein standard solution;

S3. Preparation of the sample solution: the genistin extract obtained in step S1 is made into a dilution with methanol solution C, passed through the filter membrane, and stored for later use;

S4. Detection by ultra-high performance liquid chromatography: the content of the sample obtained in step S3 is detected, and the mobile phase is pure methanol, glacial acetic acid and water to detect the content of genistin in the honey vinegar black soybean product.

Figure 1 is the UHPLC detection spectrum of the standard solution of Genistein Supplement 12.50 μg/L.