Zhishang Chemical

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Zhishang Chemical

Gastric Lipase CAS 9001-62-1

Molecular Formula: C11H9N3NaO2+

Formula Weight: 238.19783

ZSpharmac: Gastric Lipase Supplement

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Product Name: Gastric Lipase
CAS No: 9001-62-1
Purity: 99%

Basic Info

Product Name:Gastric Lipase
Other Names:Lipase
CAS:9001-62-1
Place of Origin:Shandong, China
Brand Name:ZSpharmac
Type:Food Additives
Appearance:Yellow-Brown Solution
EINECS No.:232-619-9
Storage:2-8°C
Provide:Gastric Lipase MSDS;
Gastric Lipase COA

What is Gastric Lipase?

Gastric Lipase (Lipase, glyceride hydrolase) belongs to the class of carboxyl ester hydrolase, which can progressively hydrolyze triglycerides into glycerol and fatty acids. Stomach Lipase exists in fat-containing pet, plant as well as microbial (such as mold and mildew, germs, etc) tissues. Consisting of phosphatases, sterolases and carboxylesterases. Fatty acids are extensively made use of in food, medication, leather, day-to-day chemicals, and so on. Gastric Lipase are widely located in pets, plants and also microorganisms. The various activities of Gastric Lipase depend on the characteristics of the response system, such as advertising ester hydrolysis at the oil-water interface, as well as enzymatic synthesis as well as transesterification in the organic stage.

Gastric Lipase Properties:

density 1.2
storage temp. 2-8°C
solubility H2O: 2 mg/mL, hazy with insoluble particles, faintly yellow
form solution
color yellow-brown
Water Solubility It is soluble in water.

 

Gastric Lipase Uses

  1. Gastric Lipase is commonly used in diagnostic enzymes. Quantitative analysis of serum triglycerides, prostaglandin esters, fat analysis, biochemical reagents.
  2. Gastric Lipase enzyme preparation. Mainly used in lipid modification, lipid hydrolysis and cheese manufacture, it can prevent and control the rancidity of oil and fat in chocolate and dairy products. The maximum usage amount of lecithin hydrolysis is 10000LENU/kg crude lecithin, others are used in moderation according to production needs.
  3. Gastric Lipase enzyme preparation. Mainly used in cheese manufacturing (defatting and making products with special aroma, the maximum dosage is 100mg/kg), lipid modification, lipid hydrolysis, in order to prevent the fat loss in some dairy products and chocolate.It is added to egg whites to break down fats that may be mixed in, thereby increasing its foaming power.
  4. Gastric Lipase for the food industry: has good emulsifying properties. Enhance the stability and fermentation resistance of the dough, improve the cooking quality and strength, and increase the product volume. It has whitening effect and improves the skin quality and internal color of steamed bread products. It can improve the internal structure of steamed bread, improve the appearance and texture, and improve the taste. Extend the shelf life of the product, and the re-steaming effect is good. The recommended amount is 1.0-3.0g/100kg flour (10-30ppm). b) Food flavor enhancement: By decomposing the fatty acid in the raw material, it is converted into a substance with a special aroma to increase the flavor of the product. Such as liquor brewing, dairy flavor enhancement. c) It is used to hydrolyze or degrade oils and fats in biological fermentation and animal extract processes. 2.5/1000 d) in fish fillet processing: due to the application of alkaline lipase, the large

Gastric Lipase preparation

A method for extracting and purifying Gastric Lipase by using immobilized horse antibody, comprising the steps of: S1: placing a single colony of lipase-producing Pseudomonas ATCC21808 in a 250-mL shake flask containing 25 mL of shake-flask culture solution, and at 28° C. 200rpm for 20h, then transfer into the fermentation medium at 10% of the inoculum, and cultivate for 68h at 28°C and 200rpm to obtain a fermentation broth; wherein, each liter of the shake flask broth includes: 5g of yeast extract , peptone 5g, vegetable oil 10g, the balance is water; pH value is 7.0; each liter of fermentation broth includes: yeast extract 10g, peptone 5g, NaCl 0.5g, vegetable oil 10g, the balance is water; pH value is 7.0; S2: centrifuge the fermentation broth, collect the supernatant, and obtain the crude lipase enzyme liquid; wherein, the centrifugation adopts a high-speed refrigerated centrifuge, centrifuged at 4°C and 10800g for 15 minutes; S3: immobilize the crude Gastric Lipase enzyme liquid on the Horse antibody column, during the column loading, the Gastric Lipase activity of the effluent was detected until there was Gastric Lipase activity, and the loading was stopped; then the column was washed with a 0.1M disodium hydrogen phosphate-potassium dihydrogen phosphate buffer with a pH value of 7.2 , and then eluted with 0.1M disodium hydrogen phosphate-citric acid buffer with a pH value of 3, and collected the eluate to obtain the Gastric Lipase after purification of Chemicalbook; Disodium hydrogen phosphate-potassium dihydrogen phosphate buffer is used to wash the column to neutrality, and the solution is also used to adjust the eluent to a pH value of 7; wherein, the preparation method of the immobilized horse antibody column comprises the steps of: selecting 4 Mongolian horses ( The weight and corresponding age are: 240kg and age 3 years, 290kg and 5 years old, 320kg and 7 years old, 360kg and 10 years old), each Immunization once every two weeks for a total of 12 weeks of immunization, 2 mL each time, with a dose of 15 mg Gastric Lipase plus the same amount of Freund’s complete adjuvant, subcutaneous immunization at multiple points, and the antiserum was collected after the antibody titer was more than 1:10000 detected by ELISA. ; Centrifuge the antiserum to remove cellular components and other precipitates, collect the supernatant, and purify the polyclonal antibody using a proteinA or proteinG affinity chromatography column; connect the polyclonal antibody to the activated Sepharose2B to obtain an immobilized horse antibody column; S4: adding polyethylene glycol to the purified lipase to precipitate the lipase; then centrifuging to collect the precipitate; lyophilizing the precipitate after dialysis to obtain Gastric Lipase, the enzyme activity is determined to reach 150,000-250,000 units/g ; Among them, centrifugation is centrifuged at 4°C and 000g for 8min.

Physiological Role of Gastric Lipase

At the cellular level, Gastric Lipase are confined to small cells in lysosomes. Here, it helps break down fats to aid in cell repair and regeneration. Other fat-digesting enzymes are secreted extracellularly and transported to other parts of the body as dietary catalysts. Another Gastric Lipase is also used in the hydrolysis process, helping

In fact, the human body does not only rely on fiber to break down dietary fat, the digestion process also relies heavily on the help of digestive enzymes, especially the role of Gastric Lipase in this process cannot be ignored. To be precise, without Gastric Lipase, the body would have difficulty digesting the fat content of food.

At the cellular level, Gastric Lipase are confined to small cells in lysosomes. Here, it helps break down fats to aid in cell repair and regeneration. Other fat-digesting enzymes are secreted extracellularly and transported to other parts of the body as dietary catalysts.

Another type of lipase, also known as pancreatic Gastric Lipase, is also used in the hydrolysis process to help break down fat molecules in the pancreas. When these digestive enzymes successfully break down fat, minerals from ingested food are more easily absorbed as they pass through the small intestine and eventually reach the large intestine. When pancreatic Gastric Lipase is insufficient, it can lead to digestive disorders and pancreatic disease. But when the amount is sufficient, it can speed up the rate of fat breakdown and make it easier to remove excess fat.

Because lipase is consumed by the body when it does its job, insufficient amounts are often present. One of the ways to ensure sufficient lipase in the body is to take nutritional supplements. It is best to drink some nutritional supplements before each greasy meal. They help the body produce extra lipase for a long time.

Company Profile and Corporate Culture

Company Profile:

ZhiShang Chemical is owned by ZhiShang Group is a professional new-type chemicals enterprise combined into research and development, production and sales .

The company’s competitive product is pharmaceutical raw materials and intermediates (especially carbohydrate derivatives Series), In recent years, the company has made a major breakthrough in food and feed additives, plant extraction, industrial chemicals industry .

The company insists on the spirit of “sincere management, strict quality control, customer as god” , get consistent high praise from customers at home and abroad.

Corporate Culture:

Our Mission
Help China Chemicals to benefit the happiness of human life
Our Vission
Become the most trusted chemical supplier in the world
Our Business Philosophy
Striver – oriented, enrich employees, customer first, deep service, seek development
Our Values
Be prepared for danger in times of peace, forge ahead actively, unity and cooperation, and be brave to fight

About Us

The production base is located in Zhangqiu chemical industry park and Tai’an high-tech chemical industry park. laboratory and workshop in strict accordance with the GMP standard and the product fit national ISO9001 and ISO2000 standards.

“Zhishang” products are exported to Europe, North and South America, the Middle East, Asia Pacific and Africa area, so as to establish a long-term and stable cooperation relationship with customer in the world.

Company Info
  • Business Type: Manufacturer
  • Product Range: Additive , Chemical Auxiliary & Catalyst , Organic Chemicals
  • Products/Service: Organic Intermediate,Inorganic Chemistry, APIs, Dyestuffs And Pigments, Fragrance And Spices, Food Additives
  • Total Employees: 51~100
  • Capital (Million US $): 10000000RMB
  • Year Established: 2016
Production Capacity
  • No. of Production Lines : 8
  • No. of QC Staff : 5 -10 People
  • OEM Services Provided : yes
  • Factory Size (Sq.meters) : 3,000-5,000 square meters
  • Certificate: ISO9001 , CE , GMP , API , MSDS
  • Factory Location : Diao Town Industry Park, Zhangqiu City, Jinan City, Shandong Province, China.

Service

Pre-Sales Service

* Prompt reply and 24 hours online, professional team to provide best price and high quality product.

* Sample testing support.

* Every batch of products will be tested to ensureits quality.

*The packing also can be according the customers` requirment.

*Any inquiries will be replied within 24 hours.

*we provide Commerical Invoice, Packing List, Bill of loading, COA , Health certificate and Origin certificate. If your markets have any special requirements, let us know.

 

After-Sales Service

*The fact of logistics information monitoring.

* Any questions about the product can be consulted at any time.

*Product has any problem can return.

FAQ

Do you accept sample order?

We will make samples before mass production, and after sample approved, we’ll begin mass production. Doing 100% inspection during production, then do random inspection before packing.

 

HOW TO CONFIRM THE PRODUCT QUALITY BEFORE PLACING ORDERS?

You can get free samples for some products,you only need to pay the shipping cost or arrange a courier to us and take the samples. You can send us your product specifications and requests,we will manufacture the products according to your requests.

What’s your MOQ?

Our MOQ is 1kg. But usually we accept less quantity such as 100g on the condition that sample charge is 100% paid.

Do you supply product report?

Yes. We’ll give you product analysis report before shipping.

  Is there a discount?

Different quantity has different discount.

Shipping

1. ≤50kg, Express delivery recommended, usually called as DDU service;

2. ≤500kg, Air shipping recommended, usually called as FOB, CFR, or CIF service;

3. >500kg, sea shipping recommended, usually called as FOB, CFR, or CIF service;

4. For high value products, please select air shipping and express delivery for safe.

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